The scratching-induced tunable perpendicular anisotropy in amorphous FeSiB ribbon surface has been directly observed in room temperature and air atmosphere. The magnetic stripe domain width and contrast strength can be easily tuned according to the scratch size, and the magnetic signature is erasable in nano-scale by indentation, which greatly facilitates the direct magnetic patterning. The result presents a simple way of fabricating the large-area magnetic film with controllable perpendicular anisotropy, remarkable intrinsic flexibility and high strength directly from amorphous FeSiB ribbon under ambient conditions, which may simulate much scientific and engineering interest, leading to the novel applications of metallic glasses.