A new method for dynamic calibration of the bar instrumentation, i.e. the strain measurement, of a split Hopkinson pressure bar is proposed. To this end, the displacements at the bar/specimen interfaces are measured optically and compared with the calculated displacements from the measured strain signals of the bar instrumentation. The bar signals at different positions and the displacements are analyzed by experimental and theoretical approaches. It was found that the additional measurement of the striker velocity and the assumption of momentum conservation can be omitted by applying the new method. Furthermore, there is no need for the conventional two-step calibration, i.e. ‘bars apart’ and ‘bars together’. The proposed one-step dynamic calibration of the instrumentation with respect to strains and displacements can even be performed during an actual test with a specimen. The optical extensometer is not only used for dynamic calibration purposes but also for measuring the interface displacements of the specimen during a test. Consequently, the strains can be calculated from the very beginning of the test.