Radiation Stress Research Articles

Unlocking Multifunctional Advantages with Nanocomposites Coatings for Solar Cells: A Comprehensive Review

Abstract: Nanocomposite coatings have garnered considerable attention as a versatile and innovative solution for addressing the challenges faced by solar cell technologies. This review article provides a comprehensive overview of the multifunctional advantages that nanocomposite coatings offer in the realm of solar cell technology. Furthermore, it delves into the myriad benefits that nanocomposite coatings bring to the table, including enhanced light absorption, improved charge carrier dynamics, and augmented protection against environmental factors such as moisture, UV radiation, and thermal stress. The review also discusses the diverse materials and fabrication methods employed in the development of nanocomposite coatings, highlighting their unique properties and practical applications with multifunctional benefits. Moreover, this comprehensive review explores recent advances in the field, encompassing the integration of novel nanomaterials, smart coatings, and multifunctional strategies that enable solar cells to simultaneously exhibit multiple desirable traits. Besides, the review offers insights into future prospects and challenges, presenting a roadmap for harnessing the full potential of nanocomposite coatings in solar cell technology. By unlocking the multifunctional advantages of nanocomposite coatings, this review aims to catalyze further research and innovation, ultimately advancing the prospects of sustainable and efficient solar energy generation.

CGAS Mediates the Inflammatory Responses of Human Microglial Cells to Genotoxic DNA Damage.

Genomic instability is a key driving force for the development and progression of many age-related neurodegenerative diseases and central nervous system (CNS) cancers. Recently, the cytosolic DNA sensor, cyclic GMP-AMP synthase (cGAS), has been shown to detect and respond to self-DNA accumulation resulting from DNA damaging insults in peripheral cell types. cGAS has been shown to be important in the responses of microglia to DNA viruses and amyloid beta, and we have reported that it underlies the responses of human microglia to exogenous DNA. However, the role of this cytosolic sensor in the detection of self-DNA by glia is poorly understood and its ability to mediate the cellular responses of human microglia to genotoxic DNA damage has not been established. Here, we describe the ability of ionizing radiation and oxidative stress to elicit genomic DNA damage in human microglial cells and to stimulate the production of key inflammatory mediators by these cells in an NF-kB dependent manner. Importantly, we have utilized CRISPR/Cas9 and siRNA-mediated knockdown approaches and a pharmacological inhibitor of the cGAS adaptor protein stimulator of interferon genes (STING) to demonstrate that the cGAS-STING pathway plays a critical role in the generation of these microglial immune responses to such genotoxic insults. Together, these studies support the notion that cGAS mediates the detection of cytosolic self-DNA by microglia, providing a potential mechanism linking genomic instability to the development of CNS cancers and neurodegenerative disorders.

Solution ALD Processing of Halide Perovskite Thin Films Yields Superior Functional Quality and Stability Than Classical Processing

The range of materials accessible by classical ALD is limited by the volatility requirement applied to the precursors. However, solution ALD (sALD) transfers the self-limiting surface reaction principle of ALD into the solution processing realm and allows for the same level of control in the deposition of ionic or molecular solids, such as polymers, metal-organic frameworks, and halide perovskites, which cannot be delivered from the gas phase.We have developed an sALD procedure for the direct deposition of the prototypical halide perovskite compound, methylammonium triiodoplumbate(IV), (H3CNH3)(PbI3), also referred to as methylammonium lead iodide or MAPI. The process saturates upon dosage variation to a self-limiting growth rate. The material is obtained in crystalline and highly pure, stoichiometric form at room temperature. All analytical techniques confer to indicate the absence of any lead iodide trace. The MAPI layers generated by sALD can be integrated into planar heterojunction stacks and yield functional solar cells.The direct comparison of films of several thickness values prepared in pairs by sALD and by a state-of-the-art spin-coating method shows that the sALD films always overperform their spin-coated counterparts both in terms of charge carrier lifetimes and in terms of stability towards decomposition under thermal, radiative or vacuum stress.Thus, experimental atomic-level control of solution-processed semiconductors is accessible for the first time. It can now be applied to the accurate investigation of halide perovskite photophysics and photochemistry fundamentals as they pertain to transport and interface phenomena.

Photobiomodulation effects on fibroblasts and keratinocytes after ionizing radiation and bacterial stimulus

ObjectivePhotobiomodulation therapy (PBMT) has proven to reduce inflammation and pain and increase wound healing. Thus, the aim of this study was to analyze the effects of PBMT parameters on migration, proliferation, and gene expression after ionizing radiation and bacterial-induced stress in an in vitro study. DesignKeratinocytes (HaCaT) and Fibroblasts (HGFs) were grown in DMEM with 10 % fetal bovine serum until stressful condition induction with lipopolysaccharide (LPS) of Escherichia coli (1 µg/mL), Porphyromonas gingivalis protein extract (5 µg/mL) and ionizing radiation (8 Gy). Low-laser irradiation (660 nm, 30 mW) was carried out in four sessions, with 6 h intervals, and energy density of 2, 3, 4, and 5 J/cm². Scratch assays, immunofluorescence, and RT-qPCR were performed. ResultsTreated fibroblasts and keratinocytes showed significant response in proliferation and migration after scratch assays (p < 0.05). Higher expressions of α-SMA in fibroblasts and F-actin in keratinocytes were observed in cells subjected to 3 J/cm². PI3K-pathway genes expression tended to enhance in fibroblasts, presenting a higher relative expression when compared to keratinocytes. In keratinocytes, PBMT groups demonstrated deregulated expression for all inflammatory cytokines’ genes tested while fibroblasts presented a tendency to enhance those genes expression in a dose dependent way. ConclusionsThe present study showed that delivering 660 nm, 30 mW was effective to stimulate cell migration, proliferation and to accelerate wound healing. PBMT can modulate cytokines and pathways involved in wound repair. The different energy densities delivering distinct responses in vitro highlights that understanding laser parameters is fundamental to improve treatment strategies.

Potential of UV-B radiation in drought stress resilience: A multidimensional approach to plant adaptation and future implications.

The escalating impact of climate change and ultraviolet (UV) radiation is subjecting plants to unique combinations of UV-B and drought stress. These combined stressors could have additive, synergistic, or antagonistic effects, but the precise nature of these impacts remains uncertain, hampering our ability to predict plant adaptations approach towards stressors. Our analysis of various studies shows that UV-B or drought conditions detrimentally influence plant growth and health metrics by the enhanced generation of reactive oxygen speciescausing damage to lipids, proteins, carbohydrates and DNA. Further reducing biomass accumulation, plant height, photosynthetic efficiency, leaf area, and water transpiration, while enhancing stress-related symptoms. In response to UV-B radiation and drought stress, plants exhibit a notable up-regulation of specific acclimation-associated metabolites, including proline, flavonoids, anthocyanins, unsaturated fatty acids, and antioxidants. These metabolites play a pivotal role in conferring protection against environmental stresses. Their biosynthesis and functional roles are potentially modulated by signalling molecules such as hydrogen peroxide, abscisic acid, jasmonic acid, salicylic acid, and ethylene, all of which have associated genetic markers that further elucidate their involvement in stress response pathways. In comparison to single stress, the combination of UV-B and drought induces the plant defence responses and growth retardation which are less-than-additive. This sub-additive response, consistent across different study environments, suggests the possibility of a cross-resistance mechanism. Our outlines imply that the adverse effects of increased drought and UV-B could potentially be mitigated by cross-talk between UV-B and drought regimes utilizing a multidimensional approach. This crucial insight could contribute significantly to refining our understanding of stress tolerance in the face of ongoing global climate change.

Hematopoietic Stem/Progenitor Cell Intrinsic SAM Synthesis Is Required to Prevent p53 Pathway Activation By Maintaining DNA Stability

S-adenosylmethionine (SAM) is a principle methyl group donor to maintain epigenetic status. SAM is synthesized from methionine and ATP via the enzymatic activity of Mat2a (Methionine adenosyltransferase 2a). It has been reported that SAM reduction by methionine restriction or Mat2a inhibition suppresses the proliferation of leukemia cells to attract attentions as a potential therapeutic target for leukemia. To develop the therapy targeting methionine metabolism in leukemia, it is essential to elucidate the significance of methionine metabolism in non-leukemic hematopoiesis. However, still little is known about the importance of SAM synthesis in hematopoiesis, including hematopoietic stem/progenitor cells (HSPCs), in vivo. By using Mx1-Cre mediated Mat2a gene deletion in mice ( Mat2a fl/fl;Mx1-Cre mice), we investigated the roles of Mat2a in hematopoiesis in vivo. Reduction of Mat2a activity in Mat2a knockout (KO) mice in bone marrow (BM) was confirmed by ultra-high performance liquid chromatography tandem mass spectrometry analysis. Mat2a KO mice displayed severe pancytopenia in peripheral blood (PB). Mature cell fractions (Myeloid cells, B cells and T cells) and HSPCs (HSCs, MPP2s, MPP3s and MPP4s) in BM were also significantly decreased in Mat2a KO mice, suggesting that Mat2a is essential for the maintenance of hematopoiesis from HSPCs in vivo. To exclude the possible contributions of the effects induced by Mat2a KO in non-hematopoietic cells, we performed BM transplantation analyses in which hematopoietic cell specific KO or non-hematopoietic cell specific KO were achieved. These analyses clearly confirmed that HSPC intrinsic SAM synthesis is inevitable for the maintenance of hematopoiesis. To reveal the mechanistic insights of the regulatory roles for Mat2a in HSPCs, we performed RNA sequencing analysis ( Mat2a WT vs KO in HSPCs). GSEA (Gene Set Enrichment Analysis) using the Hallmark gene sets showed that p53 pathway was most highly enriched in Mat2a KO. In depth, genes downstream of p53 ( Bax, Apaf1, Bbc3, Casp3, Casp8, Fas, Cdkn1a etc.) were up-regulated by Mat2a KO, causing cell cycle arrest and apoptosis in HSPCs. On the other hand, gene expressions of the upstream of p53 ( Atr, Atm, Chek1, Chek2 etc.) were unchanged, suggesting that p53 activity was not increased at mRNA expression levels. In line with this, mRNA expression levels of Trp53 was unchanged, but total protein amount of p53 was increased in Mat2a KO HSPCs. Since DNA stability is known as a critical regulator of p53 pathway, we analyzed gH2AX levels as DNA damage marker and found that gH2AX levels were increased in Mat2a KO HSPCs. To be noted, expressions of genes involved in Fanc pathway ( Fanca, Fancc, Fancd2, Fance, Fancg, Fancl, Fanci etc), which is responsible for the DNA maintenance, were broadly down-regulated in Mat2a KO HSPCs at transcriptomic levels. Therefore, the Fanc pathway repression by SAM synthesis inhibition could cause DNA damage and p53 pathway dependent loss of maintenance in Mat2a KO HSPCs. To further understand the importance of SAM synthesis under stress hematopoiesis. We analyzed Mat2a expression changes in HSPCs by irradiation (5.5 Gy/mouse). Intriguingly, Mat2a expression was up-regulated by irradiation. We also found that dietary methionine restriction (MR) in mice prevents its hematopoietic recovery from irradiation stress. To be noted, long term MR (24 weeks) did not show any particular compared to control diet. Therefore, methionine metabolism possesses significant roles at least in the situation where acute hematopoietic recovery is required. Collectively, in this study, we found that cell intrinsic SAM synthesis is required for the maintenance (proliferation and survival) of HSPCs in vivo. SAM might be required for the prevention of DNA damage and p53 pathway activation by supporting Fanc pathway gene expressions. Methionine and/or SAM availability might possess critical roles for the regeneration of non-leukemic hematopoiesis. Further investigation about the significance of SAM synthesis in non-leukemic hematopoiesis is required for the proper establishment of SAM synthesis targeting therapy in cancers.

Haberlea rhodopensis Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression.

Ionizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise the risk of metabolic dysfunction, accumulation of harmful mutations, genome instability, cancer, accelerated cellular senescence, and even death. Here, we present an investigation of HeLa cancer cells' early response to gamma IR (γ-IR) and oxidative stress after preincubation of the cells with natural extracts of the resurrection plant Haberlea rhodopensis. In light of the superior protection offered by plant extracts against radiation and oxidative stress, we investigated the cellular defence mechanisms involved in such protection. Specifically, we sought to evaluate the molecular effects of H. rhodopensis extract (HRE) on cells subjected to genotoxic stress by examining the components of the redox pathway and quantifying the transcription levels of several critical genes associated with DNA repair, cell cycle regulation, and apoptosis. The influence of HRE on genome integrity and the cell cycle was also studied via comet assay and flow cytometry. Our findings demonstrate that HREs can effectively modulate the cellular response to genotoxic and oxidative stress within the first two hours following exposure, thereby reducing the severity of such stress. Furthermore, we observed the specificity of genoprotective HRE doses depending on the source of the applied genotoxic stress.

Role of the NLRP1 inflammasome in skin cancer and inflammatory skin diseases.

Inflammasomes are cytoplasmic protein complexes that play a crucial role in protecting the host against pathogenic and sterile stressors by initiating inflammation. Upon activation, these complexes directly regulate the proteolytic processing and activation of proinflammatory cytokines interleukin (IL)-1β and IL-18 to induce a potent inflammatory response, and induce a programmed form of cell death called pyroptosis to expose intracellular pathogens to the surveillance of the immune system, thus perpetuating inflammation. There are various types of inflammasome complexes, with the NLRP1 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-1) inflammasome being the first one identified and currently recognized as the predominant inflammasome sensor protein in human keratinocytes. Human NLRP1 exhibits a unique domain structure, containing both an N-terminal pyrin (PYD) domain and an effector C-terminal caspase recruitment domain (CARD). It can be activated by diverse stimuli, such as viruses, ultraviolet B radiation and ribotoxic stress responses. Specific mutations in NLRP1 or related genes have been associated with rare monogenic skin disorders, such as multiple self-healing palmoplantar carcinoma; familial keratosis lichenoides chronica; autoinflammation with arthritis and dyskeratosis; and dipeptidyl peptidase 9 deficiency. Recent research breakthroughs have also highlighted the involvement of dysfunctions in the NLRP1 pathway in a handful of seemingly unrelated dermatological conditions. These range from monogenic autoinflammatory diseases to polygenic autoimmune diseases such as vitiligo, psoriasis, atopic dermatitis and skin cancer, including squamous cell carcinoma, melanoma and Kaposi sarcoma. Additionally, emerging evidence implicates NLRP1 in systemic lupus erythematosus, pemphigus vulgaris, Addison disease, Papillon-Lefèvre syndrome and leprosy. The aim of this review is to shed light on the implications of pathological dysregulation of the NLRP1 inflammasome in skin diseases and investigate the potential rationale for targeting this pathway as a future therapeutic approach.

Improving storm surge simulations by considering wave-steepness-dependent drag coefficient in the northern East China Sea

The northern East China Sea (NECS) is a semienclosed basin that is frequently attacked by tropical cyclones (TCs), and the accompanied storm surges are huge threats to coastal regions. In this study, numerical simulations of storm surges in the NECS are performed in the impacts of wave-induced surface drag (wave drag) and wave radiation stress (wave setup). The wave drag is estimated using a wave-steepness-dependent roughness parameterization. Comparisons with the observations reveal that the numerical simulations can be significantly improved by considering enhanced wave drag. The wave drag accounts for 15–22 % increase in the peak storm surge in the southern coastal regions of the Bohai Sea, which is much more significant than the wave setup (accounting for less than 10 % increase). Analysis with the wind and wave conditions shows that the significant impact of wave drag is attributed to the shoaling of young fetch-limited waves, which is accumulative toward the shoreline. The impact of different roughness parameterizations, such as wave-age-dependent scaling, is discussed. The results highlight the necessity of considering the influence of wave-enhanced surface drag on surge level in fetch-limited shoaling waters.

Radiation impacts gene redundancy and biofilm regulation of cryoconite microbiomes in Northern Hemisphere glaciers

BackgroundGlaciers harbor diverse microorganisms adapted to extreme conditions with high radiation, fluctuating temperature, and low nutrient availability. In glacial ecosystems, cryoconite granules are hotspots of microbial metabolic activity and could influences the biogeochemical cycle on glacier surface. Climate change could influence glacier dynamics by changing regional meteorological factors (e.g., radiation, precipitation, temperature, wind, and evaporation). Moreover, meteorological factors not only influence glacier dynamics but also directly or indirectly influence cryoconite microbiomes. However, the relationship of the meteorological factors and cryoconite microbiome are poorly understood.ResultsHere, we collected 88 metagenomes from 26 glaciers distributed in the Northern Hemisphere with corresponding public meteorological data to reveal the relationship between meteorological factors and variation of cryoconite microbiome. Our results showed significant differences in taxonomic and genomic characteristics between cryoconite generalists and specialists. Additionally, we found that the biogeography of both generalists and specialists was influenced by solar radiation. Specialists with smaller genome size and lower gene redundancy were more abundant under high radiation stress, implying that streamlined genomes are more adapted to high radiation conditions. Network analysis revealed that biofilm regulation is a ubiquitous function in response to radiation stress, and hub genes were associated with the formation and dispersion of biofilms.ConclusionThese findings enhance our understanding of glacier cryoconite microbiome variation on a hemispheric scale and indicate the response mechanisms to radiation stress, which will support forecasts of the ecological consequences of future climate change.3hvtG_8RFcL8puNgr6T9zaVideo

Wave–Tide Interaction by Typhoon Ampil on Wave and Storm Surge in the Changjiang River Estuary and Its Adjacent Coastal Areas

The study used the SCHISM ocean model combined with the WWM III wind wave model to quantify the interaction between wind waves and tides in the coastal zone of the Changjiang River Estuary and its adjacent areas. The wave and storm surge during Typhoon Ampil, which made landfall in July 2018 in Shanghai, were simulated by using the Climate Forecast System Version 2 (CFSv2) and Medium-Range Weather Forecasts (ECMWF) latest reanalysis (ERA5) wind dataset from 1 July to 31 July. Model results with CFSv2 forcing show better performance in terms of significant wave height and storm surge than those with ERA5 forcing. To investigate the interactions between waves, water levels, currents, and their combined effects on significant wave and surge variations, six numerical sensitivity experiments were designed according to the different coupling methods between SCHISM and WWMIII. The research shows that in coastal areas with water depths of less than 10 m, waves are affected by water levels and currents. The differences in the effect on significant wave height between wave-tide coupling and one-way coupling with water levels and currents are negligible. Wave setup is an important physical term which cannot be ignored during the variations of storm surge caused by Typhoon Ampil. The contributions of wave set up were concentrated in coastal areas with water depths less than 10 m. The peak wave setup occurred in the Changjiang River Estuary, reaching 0.15 m. In Xiangshan Bay and Sanmen Bay, wave radiation stress makes the proportion of wave setup to the total surge reached more than 30%. The consideration of wave-tide interaction can effectively improve the accuracy of numerical wave and storm surge simulations, which can provide more accurate hindcasts of wave and storm surge variations in the Changjiang River Estuary and its adjacent coastal areas.

Pre- and post-melatonin mitigates the effect of ionizing radiation-induced damage in wheat by modulating the antioxidant machinery

As an indolamine, melatonin (C13H16N2O2) performs essential roles in the regulation of plant growth and development and ameliorates the harmful effects of abiotic stresses. This study examined two types of melatonin application, pre-sowing (prMel) and application during growth (ptMel), in wheat (Triticum aestivum L.) seedlings exposed to four different doses (100, 200, 300, and 400 Gy) of radioactive cobalt (60Co) gamma rays as dry seeds to investigate their ameliorative effects on ionizing radiation (IR) stress. Peroxidase, catalase, superoxide dismutase, ascorbate peroxidase, glutathione reductase, mono- and dihydroxyperoxidase, and phenylalanine ammonia-lyase activities, and levels of lipid peroxidation, H2O2, and total glutathione (GSH), and phenolic acids (PHAs) in soluble free, ester, glycoside and ester-bound forms were examined in the seedlings. Both melatonin applications were found to increase lipid peroxidation, H2O2, and GSH contents previously reduced by gamma irradiation. The IR treatment-induced increases in enzyme activities were significantly reduced by melatonin applications. The study findings indicated that high doses of IR resulted in significant decreases in the activity and levels of the measured traits. The predominant PHAs in the tissues were vanillic, ferulic, and p-coumaric acids. In addition, ptMel application combined with IR stress lowered the total phenolic acid contents in the soluble forms while increasing those in the cell wall-bound form. In conclusion, the antioxidant system in the seedlings exposed to the different gamma ray doses was regulated by prMel and ptMel applications in such a manner as to alleviate IR stress-induced oxidatives damages in the wheat.

Deformation of nucleated cells driven by ultrasonic standing waves

The deformation of nucleated cells driven by ultrasonic standing waves in fluid environment is investigated by theoretical analysis and numerical simulation. The nucleated cells are considered to consist of cell membrane, cytoplasm and nucleus The cell membrane is assumed to have in-plane deformation and bending resistances, in which the in-plane deformation is modeled by the generalized Hooke's law, and the bending resistance is modeled by the Helfrich bending energy formula. Due to the acoustic mismatch between the media inside and outside the cell, the generated acoustic radiation stress on the cell membrane causes the nucleated cell to deform. Considering the acoustic scattering of the nucleus, the acoustic radiation stress is formulated by applying the acoustic radiation stress tensor theory. Based on the proposed theoretical model, a finite element model is established to solve the coupling problem of ultrasonic propagation and cell deformation. The analytical solution for the acoustic induced deformation of nucleated cells in the limits of long wavelength and small deformation is given and successfully verifies the finite element model. The results show that the deformation of nucleated cells is much larger than that of enucleated cells due to the effect of the nucleus on wave propagation. The deformation of nucleated cells is significantly affected by nuclear size and nuclear density. This study is helpful to accurately extract the mechanical properties of nucleated cells and further promote the detection of cell-related diseases by acoustic deformation technology.

Study on Numerical Modeling for Pollutants Movement Based on the Wave Parabolic Mild Slope Equation in Curvilinear Coordinates

Nearshore waves and currents induced by breaking of obliquely incident waves are important dynamic factors that significantly affect pollutants movement at offshore zones. A combined numerical model in orthogonal curvilinear coordinates is developed to simulate pollutants movement in surf zones, including a wave transformation model based on the parabolic mild slope equation, a wave-induced current model, and a pollutant transport model driven by waves and currents. This combined model has been applied to pollutants movement laboratory cases, and comparison of the simulations with experimental measurements shows good agreement. The model has also been applied to simulation of the Gourlay experiment and it shows consistent results with the distribution of waves and offshore currents. This coupled numerical model has preliminarily improved the situation of rare numerical research on the nearshore pollutant transport in coastal wave and wave-induced current based on wave radiation stress theory in orthogonal curvilinear coordinates.

Encapsulation of α-Lipoic Acid in Halloysite Nanotubes

A nanocomposite material based on halloysite and α-lipoic acid was studied. The kinetics of the degradation process of α-lipoic acid under the influence of ultraviolet radiation and thermal stress in its native state and in the composition of a halloysite-based nanocomposite were studied. The concentration of undegraded α-lipoic acid and the effect of the nanocomposite composition were determined by the HPLC method. It has been shown that adding α-lipoic acid to halloysite using a vacuum method allows for an increase in its resistance toward UV light of 84.4%, and the thermal resistance was also significantly improved. The composite based on halloysite and α-lipoic acid can be used to improve the resistance to photodegradation of pharmaceutical drugs or sunscreen cosmetics because this strategy preserves the biological active properties and shelf life of the α-lipoic acid.

MODELING INFRA-GRAVITY WAVES USING SCHISM-WWMIII BASED ON IMPROVED FORMULAS AND COUPLING APPROACH

The two-way coupled phase-resolved long wave (LW) model and phase-averaged spectrum wave (SW) model is often employed to simulate the coastal flooding due to superimposed astronomical high tides and storm surges. This study suggests an alternative approach by proposing a new formula for the radiation stresses (RS) term to consider the IG waves. The new formula becomes space-time dependent, and it can reduce to the original form of RS and that corresponding to the ‘surf-beats’ (Longuet-Higgins and Stewart, 1964). The new formula is programmed and implemented in the model suite SCHISM-WWMIII (Zhang, et al., 2016), which is used to simulate short wave groups induced bound and free long waves on variable water depth. The good agreement between numerical results and theoretical predictions (Liu, 1989) indicates that the new formula and coupling approach can be accurately used to describe the scattering of the IG waves in coastal waters.

Molecular Mechanisms of Resistance to Ionizing Radiation in S. cerevisiae and Its Relationship with Aging, Oxidative Stress, and Antioxidant Activity.

The repair of the damage produced to the genome and proteome by the action of ionizing radiation, oxidizing agents, and during aging is important to maintain cellular homeostasis. Many of the metabolic pathways influence multiple processes. In this way, this work aims to study the relationship between resistance/response to ionizing radiation, cellular aging, and the response mechanisms to oxidative stress, free radicals, reactive oxygen species (ROS), and antioxidant activity in the yeast S. cerevisiae. Systems biology allows us to use tools that reveal the molecular mechanisms common to different cellular response phenomena. The results found indicate that homologous recombination, non-homologous end joining, and base excision repair pathways are the most important common processes necessary to maintain cellular homeostasis. The metabolic routes of longevity regulation are those that jointly contribute to the three phenomena studied. This study proposes eleven common biomarkers for response/resistance to ionizing radiation and aging (EXO1, MEC1, MRE11, RAD27, RAD50, RAD51, RAD52, RAD55, RAD9, SGS1, YKU70) and two biomarkers for response/resistance to radiation and oxidative stress, free radicals, ROS, and antioxidant activity (NTG1, OGG1). In addition, it is important to highlight that the HSP104 protein could be a good biomarker common to the three phenomena studied.

Nonlinear analytical solution for radiation stress of higher-order Stokes waves on a flat bottom

The radiation stress of water surface waves plays an important role in many aspects of marine hydrodynamics. The nonlinear effects of highly nonlinear waves on the radiation stress were investigated on a flat bottom under finite water depth and deep water conditions. Analytical solutions for radiation stress were derived based on different order nonlinear Stokes wave theory, respectively. The results indicate that the nonlinear radiation stresses are significantly greater than those based on the linear wave, and they increase as the nonlinearity strengthens. Through an inter-comparison of the expressions of nonlinear radiation stress of various orders of Stokes waves, it turned out that the results based on the second-order Stokes wave overestimated the radiation stress, while the expressions based on Fenton's fifth-order Stokes wave were more reasonable in reflecting the nonlinear effect of waves. In the case of extreme wave steepness, the fifth-order Stokes wave radiation stresses Sxx5 was approximately 17% larger than that of the linear wave. Finally, using the expressions of the fifth-order Stokes wave radiation stress, bound infragravity waves excited by the bichromatic wave groups formed by the superposition of two fifth-order Stokes waves were obtained.

Resilience and Mitigation Strategies of Cyanobacteria under Ultraviolet Radiation Stress.

Ultraviolet radiation (UVR) tends to damage key cellular machinery. Cells may adapt by developing several defence mechanisms as a response to such damage; otherwise, their destiny is cell death. Since cyanobacteria are primary biotic components and also important biomass producers, any drastic effects caused by UVR may imbalance the entire ecosystem. Cyanobacteria are exposed to UVR in their natural habitats. This exposure can cause oxidative stress which affects cellular morphology and vital processes such as cell growth and differentiation, pigmentation, photosynthesis, nitrogen metabolism, and enzyme activity, as well as alterations in the native structure of biomolecules such as proteins and DNA. The high resilience and several mitigation strategies adopted by a cyanobacterial community in the face of UV stress are attributed to the activation of several photo/dark repair mechanisms, avoidance, scavenging, screening, antioxidant systems, and the biosynthesis of UV photoprotectants, such as mycosporine-like amino acids (MAAs), scytonemin (Scy), carotenoids, and polyamines. This knowledge can be used to develop new strategies for protecting other organisms from the harmful effects of UVR. The review critically reports the latest updates on various resilience and defence mechanisms employed by cyanobacteria to withstand UV-stressed environments. In addition, recent developments in the field of the molecular biology of UV-absorbing compounds such as mycosporine-like amino acids and scytonemin and the possible role of programmed cell death, signal perception, and transduction under UVR stress are discussed.

Unified analytical solution for group-induced infragravity waves based on Green's function

Short-wave group forcing is a major driving mechanism of infragravity waves. The subharmonic response to wave group forcing approaches resonance in shallow water where the group velocity is equal to the shallow-water wave-propagating speed. Currently, there is a lack of understanding of the connection between the free and bound components of group-induced infragravity waves and the consistency among existing solutions for off- and near-resonance conditions in intermediate and shallow water. Here, a unified solution of group-induced subharmonics is derived based on Green's function for the first time. The new solution is valid for any resonance intensity and is able to describe group-induced subharmonic behaviour at all water depths consistently from a new angle. The proposed solution reduces to existing solutions for intermediate depth (Longuet-Higgins &amp; Stewart, J. Fluid Mech., vol. 13, 1962, pp. 481–504; Zou, Phys. Oceanogr., vol. 41, 2011, pp. 1842–1859), shallow water and/or over a plane sloping beach (Van Leeuwen, PhD thesis, TU Delft, 1992; Schäffer, J. Fluid Mech., vol. 247, 1993, pp. 551–588; Janssen et al., J. Geophys. Res., vol. 108, 2003, p. 3252; Contardo et al., J. Phys. Oceanogr., vol. 51, 2021, pp. 1465–1487; Liao et al., J. Phys. Oceanogr., vol. 51, 2021, pp. 2749–2765). Unlike previous solutions, the Green's function-based solution describes all subharmonics as free subharmonics continuously radiated away from each point source in the group-induced forcing field determined by wave radiation stress gradients. The superposition of all these free subharmonics yields so-called bound subharmonics by previous studies due to group-modulated emission of each free subharmonic through the source field bound to the wave group. Therefore, this solution provides theoretical evidence that the group-induced subharmonic at any observation point is dependent on the surrounding radiation stress field and topography. Under full-resonance conditions in shallow water, downwave-propagating subharmonics excited at all source locations interfere with each other constructively; therefore, their superposed amplitude is proportional to the travel distance of wave groups. Combined with the conventional moving-breakpoint forcing model, the predicted amplitude of the subharmonic in the surf zone by the present solution is in good agreement with laboratory observations.