Streptozotocin-induced Diabetic Male Rats Research Articles

Lead Nitrate Induced Testicular Toxicity in Diabetic and Non-Diabetic Rats: Protective Role of Sodium Selenite

Among heavy met als, lead is one of the common pollutants found in the environment and biological system. In the present study, streptozotocin-induced diabetic and normal non-diabetic male Wistar rats were given sodium selenite (1.0 mg/kg bw), lead nitrate (22.5 mg/kg bw) and sodium selenite plus lead nitrate (1.0 mg/kg+22.5 mg/kg bw, respectively) through gavage. At the end of 4th week, malondialdehyde (MDA) levels, antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST)], and histopathological changes of testes were investigated compared to the control group. No significant differences were observed between the control and sodium selenite treated groups. However, lead nitrate increased the levels of MDA, SOD, CAT, GPx and GST activities compared with the control group in diabetic and non-diabetic rats. Light microscopic analyses revealed that lead nitrate induced numerous histopathological changes in testis tissues of diabetic and non-diabetic rats. In the diabetic and non-diabetic sodium selenite plus lead nitrate treated groups, there were statistically significantly decreased MDA levels and antioxidant enzymes activities and mild pathological changes. As a result, sodium selenite significantly reduced lead nitrate induced testicular toxicity for both diabetic and non-diabetic rats.

Modulation of antioxidant status in streptozotocin-induced diabetic male wistar rats following intake of red palm oil and/or rooibos

ObjectiveTo investigate the role of red palm oil (RPO), rooibos tea extract (RTE) and their combined treatment (RPO + RTE) on antioxidant status in streptozotocin (STZ)-induced diabetic rats. MethodsDiabetes mellitus was induced by a single administration of streptozotocin (50 mg/kg) and the rats were treated for 7 weeks. Antioxidant enzymes [catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)], antioxidant capacity [trolox equivalence antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC)] as well as total protein, albumin, globulin, total glutathione, conjugated diene and thiobarbituric acid reactive substances (TBARS) were investigated. ResultsTreatment with RPO, RTE and RPO + RTE significantly (p>0.05) improved liver SOD and plasma ORAC in the diabetic rats. Similarly, diabetic rats treated with RTE and RPO + RTE enhanced liver GPx. A significant (P<0.05) increase in the plasma TBARS in the diabetic control group was observed when compared with the normal control group. Treatment of diabetic rats with RTE and RPO + RTE reduced plasma TBARS to a level not significantly different at P<0.05 from the normal control group. ConclusionsThe results revealed the anti-oxidative potentials of red palm oil, rooibos and their combination in diabetic conditions and hence, they could be useful in the management of diabetes and its complications.

Activation of Angiotensin Type 2 Receptors Partially Ameliorates Streptozotocin-Induced Diabetes in Male Rats by Islet Protection

We have previously demonstrated that rat islets express a high density of angiotensin type 2 receptors and that activation of this receptor evokes insulinotropic effect. In this study, we evaluated the protective effects of Compound 21 (C21), a nonpeptide angiotensin type 2 receptor agonist, on islets in streptozotocin (STZ)-induced diabetes. Rats were assigned to five groups: normal, STZ, and STZ plus C21 (0.24, 0.48, and 0.96 mg/kg·d). C21 was continually infused by a sc implanted osmotic minipump for 14 days, and STZ was bolus injected on day 7. Body weight, water intake, urine excretion, and blood glucose were monitored daily. On the last day, the rats received an oral glucose tolerance test, and the pancreata were saved to examine islet morphology and biochemical parameters of oxidative stress and apoptosis. We found that, compared with control STZ rats, C21-treated STZ rats displayed less water intake and urine excretion, lower blood glucose, higher serum insulin concentration, and improved glucose tolerance. These rats had more islets, larger islet mass, and up-regulated insulin protein and proinsulin 2 mRNA expressions in the pancreas. Their islets displayed lower superoxide, decreased gp91 expression, and increased superoxide dismutase 1 expression as well as less apoptosis and down-regulated caspase-3 expression. In the epididymal adipose tissue of these rats, we found a decreased adipocyte size and up-regulated adipocyte protein 2 expression. The protective effects of C21 on β-cells against the toxic effects of STZ were also confirmed in cultured INS-1E cells. These data suggest that C21 ameliorates STZ-induced diabetes by protecting pancreatic islets via antioxidative and antiapoptotic effects.

Effects of transdermally delivered insulin on some selected metabolic parameters of streptozotocin-induced diabetic male Sprague-Dawley rats

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

A Phytooxysterol, 28-Homobrassinolide Modulates Rat Testicular Steroidogenesis in Normal and Diabetic Rats

Steroidogenesis in testicular cells depends upon the availability of cholesterol within testicular mitochondria besides the activities of 3β-hydroxysteroid dehydrogenase (3β-HSD, 17β-hydroxysteroid dehydrogenase [17b-HSD]), and the tissue levels of steroidogenic acute regulatory protein (StAR), androgen-binding protein (ABP), and testosterone (T). Cellular cholesterol biosynthesis is regulated by endogenous oxycholesterols acting through nuclear hormone receptors. Plant oxysterols, such as 28-homobrassinolide (28-HB), available to human through diet, was shown to exhibit antihyperglycemic effect in diabetic male rat. Its role in rat testicular steroidogenesis and lipid peroxidation (LPO) was therefore assessed using normal and streptozotocin-induced diabetic male rats. Administration of 28-HB (333 µg/kg body weight) by oral gavage for 15 consecutive days to experimental rats diminished LPO, increased antioxidant enzyme, 3β-HSD and 17β-HSD activities, and elevated StAR and ABP expression and T level in rat testis. We report that 28-HB induced steroidogenesis in normal and diabetic rat testis.

Corrective role ofEugenia jambolanaon testicular impairment in streptozotocin-induced diabetic male albino rat: An approach through genomic and proteomic study

The present study was conducted to explore the effect of ethyl acetate fraction of hydro-methanolic (40 : 60) extract of seed of Eugenia jambolana on testicular impairment in diabetic rats. In this respect, biomarkers of oxidative stress, genomics and proteomics in testicular tissue were assessed. Side by side, glycated haemoglobin, serum testosterone, activities of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in serum, epididymal sperm count including reproductive organosomatic indices were evaluated. Results indicate that a significant recovery (P < 0.05) in the levels of these parameters in fraction-treated diabetic group in comparison with diabetic control. A significant recovery was noted (P < 0.05) in the expression of Bax and Bcl-2 gene towards the control after the treatment of said fraction. Histological study also focused a significant recovery (P < 0.05) in the number of different generation of germ cells at stage VII of spermatogenesis in fraction-treated diabetic group. The said fraction treatment to diabetic rat can recover the activities of serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase significantly towards the control (P < 0.05). Finally, it may be concluded that ethyl acetate fraction of seed of E. jambolana has a promiseable remedial effect on diabetes-induced testicular dysfunctions in male rat without inducing any metabolic toxicity.

Effects of Aqueous Extract of Berberis integerrima Root on Some Physiological Parameters in Streptozotocin-Induced Diabetic Rats.

Diabetes mellitus is a common endocrine disorder. Anti-diabetic agents from natural and synthetic sources are available for the treatment of this disease. Berberis integerrima is a medicinal shrub used in conventional therapy for a number of diseases. The aim of the present study was to investigate the effects of aqueous extract of Berberis integerrima root (AEBI) on some physiological parameters in normal and streptozotocin-induced (STZ-induced) diabetic male Wistar rats. STZ-induced diabetic rats showed significant increases in the levels of blood glucose, triglycerides (TG), total cholesterol (TC), low density lipoprotein LDL-cholesterol (LDL-C), creatinine (Cr), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin while body weight, high density lipoprotein HDL-cholesterol (HDL-C) and total protein levels were significantly decreased compared to normal rats. Treatment of diabetic rats with different doses of aqueous extract of Berberis integerrima root (250 and 500 mg/Kg bw) resulted in a significant decrease in blood glucose, triglycerides, cholesterol, LDL-cholesterol, ALT, AST, ALP, total bilirubin, creatinine and urea while HDL-cholesterol and total protein levels were markedly increased after six weeks compared to untreated diabetic rats. The effects of the AEBI at dose of 500 mg/Kg in all parameters except blood glucose (similar) is more than to the standard drug, glibenclamide (0.6 mg/Kg, p.o.). The results of this study indicate that the tested aqueous extract of Berberis integerrima root possesses hypoglycemic, hypolipidemic and antioxidant effects in STZ-induced diabetic rats.

Onion peel extracts ameliorate oxidative stress in streptozotocin-induced diabetic rats

Background: Onion peel extracts contain quercetin derivatives, flavonoids that have been shown to significantly improve diabetic status and to exhibit antioxidant properties in animal models. Vitamin E is an important antioxidant that is present within the cell membrane and acts as a lipid-soluble scavenger. This study aimed to compare the efficacy of an onion peel extract and vitamin E to alleviate the altered redox balance of diabetic rats. Methods: Streptozotocin-induced diabetic male Wistar rats (n = 34) were randomly divided into three treatment groups. For 45 days, the first group was fed a normal diet (diabetic control group), the second group was fed a normal diet plus 20 mg/kg body weight vitamin E and the third group was fed a normal diet plus 1% OPE . Results: The formation of malondialdehyde and protein carbonyls was significantly suppressed and the activity of superoxide dismutase was increased in different areas of the brain upon onion peel extract administration (P < 0.001) compared to the diabetic control group. Furthermore, vitamin E did not significantly decrease the level of oxidative stress or the blood glucose concentration in these rats. Conclusion: OPE is better able to ameliorate oxidative stress and hyperglycaemia than vitamin E in a diabetic rat model.

Antihyperglycemic and antioxidative effect of hydro - methanolic (2:3) extract of the seed of Swietenia mahagoni (L.) Jacq. in streptozotocin-induced diabetic male albino rat: An approach through pancreas

The goal of this study was to investigate the effects of the hydro - methanolic (2:3) extract of the seed of Swietenia mahagoni (L.) Jacq. for the management of streptozotocin (STZ)-induced diabetes. Wistar rats were divided equally into four groups (n = 6): normal control, diabetes control, diabetes + extract treated, and diabetes + metformin treated. The extract (25 mg/100 g by weight) and metformin (2.5 mg/100 g by weight) were administered once a day, orally by gavages for 21 days at fasting condition. Hexokinase, glucose-6-phosphate dehydrogenase, and glucose-6-phosphatase activities were measured in hepatic tissue. Activities of catalase (CAT), peroxidase (Px), and superoxide dismutase (SOD), along with the quantity of thiobarbituric acid reactive substances (TBARS) and conjugated diene (CD) in hepatic and renal tissues, were assessed. Histoarchitecture of the pancreas and serum insulin level were also evaluated. Significant diminution in the activities of hepatic hexokinase and glucose-6-phosphate dehydrogenase along with elevation in glucose-6-phosphatase were noted in STZ-induced animals with diabetes in respect to control animals. Level of fasting blood glucose (FBG) was elevated in animals with diabetes. Activities of CAT, Px, and SOD were diminished significantly along with the elevation in TBARS and CD levels in animals with diabetes. Diameter of pancreatic islets, count of islets, and degeneration in pancreatic acini were also noted in animals with diabetes. Treatment of these animals with extract or metformin resulted in substantial recovery in the aforementioned biosensors toward the control level. This recovery is not equal to metformin because the plant extract is not the pure form of effective ingredient(s) but provides insight to the pharmaceutical industries that the extract has a protective therapeutic effect against diabetes through ß-cell regeneration capacity.

Antihyperglycemic and antihyperlipidemic effects of guar gum on streptozotocin-induced diabetes in male rats.

Background:Herbal medicine is widely used in the treatment of diseases like diabetes mellitus. We investigated the effects of guar gum in diabetic rats for the reduction of the risk of diabetes and cardiovascular disease. Dietary pattern emphasizing foods high in complex carbohydrates and fiber are associated with low blood glucose and cholesterol levels.Materials and Methods:Diet containing 0%, 5%, 10% and 20% (w/w) guar gum was fed to diabetic rats for 28 days. Blood serum glucose, triglycerides, cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol levels, atherogenic index levels, body weights and food intake were monitored at 0, 7.14 and 28 days after induction of diabetes.Results:In spite of the fact that diabetes elevated blood lipids in all rats after 14 days, the guar gum diet significantly decreased the serum concentration of cholesterol, triacylglicerols and LDL-C and atherogenic index. The most significant result in this study was the reduction of blood glucose in diabetic rats treated with the guar gum diet after 28 days versus non- and glibenclamide-treated rats. The gum promoted a general improvement in the condition of the diabetic rats in body weight and food intake in comparison with nontreated rats.Conclusion:The results of this research suggest that guar gum was significantly effective in comparison with glibenclamide in the treatment of hyperlipidemia and hyperglycemia in diabetes rats. Therefore, it may be suggested as a reliable fiber in diabetic regimes in diabetic patients.

Antidiabetic and Antioxidant Effects of Newly Synthesized Pyrimido[1,6-A] Pyrimidine Derivatives in Neonatal Streptozotocin-Induced Diabetic Rats

Synthesis of some novel pyrimido[1,6-a]pyrimidine derivatives 4, 8 and 10 was described through the respective reactions of sodium salts of formyl ketones 1, 7 and 9 with 6-aminothiouracil. The characterization of the reaction products was confirmed by using the available elemental analysis and spectral data. One of these derivatives ( 4b), 1-thioxo-1,2,7,8,9,10-hexahydro-3H-pyrimido[1,6-a]quinazolin-3-one, was tested using sublethal dose level (10 mg/kg b. w./day for 3 weeks) and was found to have potent antihyperglycemic, antihyperlipidemic and antioxidant properties in neonatal streptozotocin-induced (n-STZ) diabetic male and female albino rats.

Ameliorative effect of astaxanthin on endothelial dysfunction in streptozotocin-induced diabetes in male rats

The present study was designed to examine whether astaxanthin (ASX, 3,3-dihydroxybeta, beta-carotene-4,4-dione, CAS 472-61-7), a dietary antioxidant carotenoid that is naturally present in algae, crustaceans, and fish, has a protective effect on endothelial dysfunction of aortas in diabetic rats and the possible molecular mechanism involved. Male Wistar rats were randomly divided into four groups: control rats, diabetic rats, diabetic rats treated with ASX (10 mg/kg/d), and control rats treated with ASX. Type 1 diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ; 60 mg/ kg). STZ-induced diabetes in rats was complicated with excessive oxidative stress and endothelial dysfunction, increased serum oxidized low-density lipoprotein (ox-LDL) and aortic malondialdehyde (MDA) levels, inhibited endothelium-dependent vasorelaxation to acetylcholine (ACh) and unaffected endothelium-dependent vasorelaxation to sodium nitroprusside (SNP). Simultaneously, lectin-like oxLDL receptor-i (LOX-1) expression was enhanced and endothelial nitric oxide (NO) synthase (eNOS) expression was reduced in the aortas of diabetic rats. ASX treatment could significantly decrease serum oxLDL and aortic MDA levels, attenuate blunted endothelium-dependent vasodilator responses to ACh, upregulate eNOS expression, and decrease LOX-1 expression. These results indicated that ASX could ameliorate diabetic endothelial dysfunction by inhibiting the ox-LDLLOX-1-eNOS pathway. Treatment with ASX might be clinically useful for diabetic complications associated with endothelial dysfunction.

Hypoglycemic and insulin mimetic impact of catechin isolated from Cassia fistula: a substantiate in silico approach through docking analysis

A study of ancient literature indicates that diabetes was fairly well known and well conceived as an entity in India. The nature has provided abundant plant wealth for all the living creatures, which possess medicinal virtues. Therefore, there is a necessity to explore their uses and to conduct pharmacognostic and pharmacological studies to ascertain their therapeutic properties. In fact, nowadays diabetes is a global problem. Hence, this study aims to open new avenues for the improvement of medicinal uses of catechin isolated from the methanol extract of Cassia fistula stem bark for the selected area of diabetes. Oral administration of catechin (20 mg/kg b.wt) for 60 days produce a better glucose tolerance pattern in Streptozotocin-induced diabetic male albino Wistar rats. Another important objective of the study is to bring the implication of computer-aided drug development. In biomedical arena, computer-aided or in silico design is being utilized to expedite and facilitate hit identification, hit-to-lead selection, optimize the absorption, distribution, metabolism, excretion and toxicity profile, and avoid safety issues. Commonly used computational approaches include ligand-based drug design (pharmacophore, a 3-D spatial arrangement of chemical features essential for biological activity), structure-based drug design (drug target docking), and quantitative structure–activity and quantitative structure–property relationships. The 3D structure of the catechin was docked with the receptors (PPARγ and insulin receptor) by Discovery Studio 2.1 version (An Accelery’s product). The results show that catechin possesses a potential agonist characteristic that is capable of activating Insulin receptor and Peroxisome proliferator-activated receptor gamma. These results confirm the hypoglycemic effect of catechin. We can conclude that catechin could be developed into potential oral hypoglycemic drug.

Continuous Electrical Stimulation of Cardiomyocytes Prevents Glucose‐Induced Contractile Dysfunction

Diabetes mellitus (DM) is a disease with a high mortality rate in patients with heart problems, and is one of the main risk factors for developing cardiovascular disease. Culturing cardiomyocytes in high glucose (HG) recapitulates the contractile dysfunction seen in myocytes isolated from type 1 diabetic animals. However, no studies have assessed the effects of continually stimulated myocytes in HG cultures. The purpose of the present study was to compare in vitro contractile function and calcium signaling in continually stimulated and quiescent ventricular myocytes cultured from control and diabetic rats. Ventricular myocytes were isolated from both control and streptozotocin-induced diabetic (55 mg/kg, i.p.) adult male Sprague-Dawley rats and stimulated overnight (0.5 and 1.0 Hz) in control or HG (25.5mM glucose) media. Sarcomeric contractile properties and calcium fluxes were evaluated using an IonOptix Field-Stimulator system, and continual electrical pacing of myocytes was performed using an IonOptix C-Pace EP Culture Pacer. Measurement of contractile function included both sarcomere and cell edge contraction at multiple frequencies. We found that maintaining contractile activity overnight in HG media did not produce contractile dysfunction present in short-term, quiescent HG culture, implicating mechanisms related to glucose metabolism.

Diesel particulate matter exposure exacerbates ROS formation and contractile dysfunction in diabetic cardiomyocytes

Two key contributors to heart disease in large cities are diesel particulate matter (DEP) pollution and diabetes mellitus (DM). The aim of the present study was to assess contractile function, calcium signaling and reactive oxygen species (ROS) formation in ventricular myocytes from control and diabetic adult rats exposed to DEP in vitro. Ventricular myocytes were isolated from both control and streptozotocin-induced diabetic adult male Sprague-Dawley rats. Sarcomeric contractile properties and calcium flux were evaluated using an IonOptix Field-Stimulator system, and intracellular ROS formation was assessed using a DCF assay. We found that several measurements of contractile function and calcium flux were reduced with DEP treatment in cells from both the control and diabetic rats, but with a greater degree in the diabetic rats. Further, our studies indicated that co-culture with antioxidants alleviated dysfunction in DEP-treated myocytes. Our final data confirmed that the effects of DEP on the function of the myocyte are exacerbated in cells from the diabetic heart.

Evaluation of antidiabetic, antihyperlipidemic and antioxidant effects of Boehmeria nivea (L.) Gaudich., Urticaceae, root extract in streptozotocin-induced diabetic rats

The potential role of 80% methanolic extract of Boehmeria nivea (L.) Gaudich., Urticaceae, root in the treatment of diabetes, along with its antihyperlipidemic and antioxidant effects, was studied in streptozotocin-induced diabetic male Wistar rats. Preliminary screening of the extract revealed the presence of polyphenolics and flavonoids. The animal study was conducted with variable doses of 125, 250 and 500 mg/kg of extract for 21 days in diabetic rats. A significant effect was observed at a dose of 500 mg/kg, which was comparable to the standard drug, glibenclamide. Administration of the extract at a 500 mg/kg dose resulted in a significant reduction of fasting blood glucose, total cholesterol, triglycerides, blood urea, alanine aminotransferase, aspartate aminotransferase, urine sugar and urine ketone levels in diabetic rats in comparison with the diabetic control group. Additionally, this dose significantly increased body weight, hemoglobin, plasma total protein, high density lipoprotein cholesterol, liver glycogen content, superoxide dismutase, reduced glutathione and catalase levels in diabetic rats at the end of 21 days of treatment. Therefore, dietary supplementation with Boehmeria nivea root extract could be beneficial for correcting hyperglycemia, hyperlipidemia and enhancing the antioxidant defense system.

Beneficial effects of quercetin on sperm parameters in streptozotocin‐induced diabetic male rats

Quercetin (QR) is a strong antioxidant and has been shown to reduce oxidative stress in the long-term treatment of streptozotocin (STZ)-induced diabetes in animal models. Antioxidants have significant effects on spermatogenesis, sperm biology and oxidative stress, and changes in antioxidant capacity are considered to be involved in the pathogenesis of chronic diabetes mellitus. The present study aims to examine the influence of QR on spermatogenesis in STZ-induced diabetes in male Wistar rats. Animals (n = 50) were allocated into five groups: Group 1: Control rats given 0.5 ml of 20% glycerol in 0.9% normal saline. Group 2: Control rats given buffer (pH4.0).Group 3: diabetic controls. Group 4: rats given QR 15 mg/kg/day (i.p.). Group 5: STZ + QR rats. Animals were kept in standard conditions. At the end of the experiment (28th day), blood samples were taken for determination of testosterone, total antioxidant capacity, and levels of malondialdehyde and oxidized low-density lipoprotein. All rats were euthanized, testes were dissected out and spermatozoa were collected from the epididymis for analysis. Sperm numbers, percentages of sperm viability and motility, and total serum testosterone increased significantly in QR-treated diabetic rats (P < 0.05) compared with control groups. In histopathology, degeneration and inflammation in testes cells associated with diabetes were improved and testes weights in the QR-treated diabetic group decreased significantly in comparison with controls (P < 0.05). We conclude that QR has significant beneficial effects on the sperm viability, motility, and serum total testosterone and could be effective for maintaining healthy sperm parameters and male reproductive function in diabetic rats.

3-Aryl-2-{4-[4-(2,4-dioxothiazolidin-5-ylmethyl)phenoxy]-phenyl}-acrylic acid alkyl ester: synthesis and antihyperglycemic evaluation

A number of 2,4-thiazolidinedione derivatives of aryl-substituted cinnamic acid were synthesized and studied for their antihyperglycemic activity in neonatal streptozotocin-induced diabetic Wister male rats. Substitution of the aryl ring resulted in higher activity. 3-(2,4-Dimethoxyphenyl)-2-{4-[4-(2,4-dioxothiazolidin-5-ylmethyl)phenoxy]-phenyl}-acrylic acid methyl ester (Thz7), was found to be most active compound in this series which showed that disubstitutions on aryl ring by electron releasing groups were found suitable for promising antihyperglycemic activity. Increase in the ester carbon chain length decreased the activity.

Cloves protect the heart, liver and lens of diabetic rats

Clove oil was previously reported to modulate physiological responses in streptozotocin-induced diabetic rats. This study attempts to evaluate the in vivo organ and tissue protective effects of dietary cloves ( Eugenia aromaticum) in chronic hyperglycaemia. The cloves (equivalent to 100 mg total eugenol + eugenyl acetate per kg body weight/day) were administered orally to streptozotocin-induced diabetic male Sprague-Dawley rats. Fasting blood glucose levels, as well as organ tissue physical and biochemical markers, were monitored. Dietary supplementation with cloves reduced tissue injuries, especially in the lens and cardiac muscles, and to a lesser extent in the liver but not the kidneys. Additionally, the cloves treatment significantly reduced blood sugar increases and lipid peroxidation in streptozotocin-induced diabetic rats by restoring the antioxidant enzyme levels. Cloves inhibited hyperglycaemia-induced oxidative tissue damage and cataract formation in the eye lens. This study demonstrates the in vivo antioxidative organ protective effects of clove in diabetics.

Effect of Diashis, a polyherbal formulation, in streptozotocin-induced diabetic male albino rats.

This study focuses on the effect of ‘Diashis’, a polyherbal formulation composed of eight medicinal plants for the management of streptozotocin (STZ)-induced diabetes in rats. As oxidative stress is one of the consequences of diabetes, the activities of hepatic antioxidant enzymes and metabolic enzymes were evaluated. Treatment with ‘Diashis’ in STZ-induced diabetic rats resulted in a significant (P < 0.01) recovery in the activities of hepatic hexokinase, glucose-6-phosphate dehydrogenase, and glucose-6-phosphatase along with correction in the levels of fasting blood glucose, glycated hemoglobin, and liver and skeletal muscle glycogen. The oxidative stress status in the liver was corrected by ‘Diashis’ which was highlighted by the recovery in the activities of catalase, peroxidase, and glutathione-S-transferase along with the correction in the quantity of thiobarbituric acid-reactive substances and conjugated diene. ‘Diashis’ was not found to have any metabolic toxicity. The antidiabetic effects of ‘Diashis’ were compared with those of the antidiabetic drug, ‘Glibenclamide’.