Abstract
Two key contributors to heart disease in large cities are diesel particulate matter (DEP) pollution and diabetes mellitus (DM). The aim of the present study was to assess contractile function, calcium signaling and reactive oxygen species (ROS) formation in ventricular myocytes from control and diabetic adult rats exposed to DEP in vitro. Ventricular myocytes were isolated from both control and streptozotocin-induced diabetic adult male Sprague-Dawley rats. Sarcomeric contractile properties and calcium flux were evaluated using an IonOptix Field-Stimulator system, and intracellular ROS formation was assessed using a DCF assay. We found that several measurements of contractile function and calcium flux were reduced with DEP treatment in cells from both the control and diabetic rats, but with a greater degree in the diabetic rats. Further, our studies indicated that co-culture with antioxidants alleviated dysfunction in DEP-treated myocytes. Our final data confirmed that the effects of DEP on the function of the myocyte are exacerbated in cells from the diabetic heart.
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