Growth Of Streptococcus Mutans Research Articles

Effectiveness of Butterfly Pea ( Clitoria Ternatea ) Extract Against Streptococcus Mutans Bacterial Growth In Vitro

Dental caries is still a major problem in dentistry and is the most common infectious disease in children. Caries is a microbiological infectious disease that can damage the hard tissue of the teeth. This study aims to determine the effectiveness of butterfly pea flower extract ( Clitoria ternatea ) on the growth of Streptococcus mutans in vitro. This type of research is laboratory experimental research using a post test only control group design approach The samples used were butterfly pea flower extract ( Clitoria ternatea ) diluted with DMSO to a concentration of 100%, 50%, 25%, 12.5%, 3.125%, 6.125% and Streptococcus mutans ATCC 25175. This research was conducted using the MIC test dilution method and KBM to see the effectiveness of the antibacterial extract against the test bacteria. Analysis of research data using the one way ANOVA test (p <0.05) to see significant differences between study groups with the number of test bacteria, followed by a double comparison test with the Post Hoc Least Significance Different (LSD) method to determine the average difference between treatment groups. Antibacterial effectiveness test results showed that butterfly pea flower extract ( Clitoria ternatea ) was effective in inhibiting Streptococcus mutans bacteria (p<0.05), at a concentration of 25% was the minimum inhibitory level (MIC) and 50% concentration was the minimum killing rate (MBC). ). The conclusion of this study is that the extract of butterfly pea flower ( Clitoria ternatea ) is effective in inhibiting and killing Streptococcus mutans bacteria.

Effect of Katuk Leaves Ethanol Extract Gel as an Antibacterial and Antifungal in Orthodontic Treatment

Fixed orthodontic treatment is one option for treating malocclusion. The intricate design makes it difficult for patients to clean their teeth and can cause the emergence of microorganisms in the oral cavity. Katuk plant (Sauropus androgynus (L.) Merr.) is commonly used as herbal medicine. Flavonoids in katuk leaves have antimicrobial properties. The purpose of this study was to determine the effect of katuk leaf ethanol extract gel as an antimicrobial by inhibiting the growth of bacteria and fungi involved in orthodontic treatment. The study was an experimental study with a post-test-only control group using katuk leaves ethanol extract gel formulation as treatment, aquadest gel as a negative control, ketoconazole as a positive control against Candida albicans, and amoxicillin against Streptococcus mutans. Data were analyzed using the Kruskall– Wallis and Mann–Whitney tests (P < 0.05). The results show that katuk leaves have inhibition on Streptococcus mutans (P = 0.000) growth but have no inhibition on Candida albicans (P = 1.00) growth. The conclusion of this study is that the gel of katuk leaves ethanol extract (S. androgynus (L.) merr.) has antibacterial activity against S. mutans in the oral cavity but no antifungal effect on C. albicans. Keywords: Candida albicans, fixed orthodontics, katuk leaves, Streptococcus mutans

Metabolit Sekunder dan Aktivitas Antibakteri Ekstrak Metanol Daun Kamboja Putih (Plumeria acuminata Ait) Terhadap Beberapa Bakteri Uji

Kamboja (Plumeria acuminata Ait) plant is traditional medicine used as medication for ulcer and abscess. Steroid and tanin is component of chemical compound in kamboja leaves that supposed to have antibacterial activity. This study aims to determine the content of secondary metabolites and effective concentration of methanol extracts of kamboja leaves as antibacterial. Identification contains of secondary metabolites by used reagent Mayer, Dragendorf, Liebermann-Burchard, FeCl, Mg powder and HCl. Analysis of secondary metabolites characterized by the presence of sediment or froth. The antibacterial activity test carried out by agar diffusion method. Inhibition zone is analyzed by Anava and Duncan test. Secondary metabolite the compound containing steroid and tanin. The effective concentration of 40% were significantly different with a value of p = 0.00 (p<0.05) on the growth of Streptococcus mutans and Staphylococcus aureus bacteria and a concentration of 30% is very significantly different from the value of p = 0.00 (p<0.05) on the growth of Escherichia coli bacteria in vitro.

Antibacterial activity of coriander seeds extract (Coriandrum sativum) against the growth of Streptococcus mutans

Background: Streptococcus mutans is the main cause of dental caries, previously known as part of the normal flora in the oral cavity which plays a role in the process of fermenting carbohydrates to produce acid, and causes tooth demineralization. Chemical-based medicinal products such as toothpaste and mouthwash. Continuous use of chemicals can cause side effects for users such as hypersensitivity, therefore an alternative treatment from natural ingredients such as coriander seeds which can inhibit bacterial growth is needed. Objective: This research aims to determine the lowest concentration of coriander seed extract in 0,75%, 1.5%, 3% inhibiting the growth of Streptococcus mutans. Methods: This study was conducted by soaking the test discs in coriander seed extract concentrations of 0.75%, 1.5% and 3%. each disc was inserted into the bacterial culture to be cultured, incubated and seen whether there was a clear zone or not. Result: Test results showed a clear zone at a concentration 1.5% with an average diameter of 1.5 mm, at concentration of 3% an average diameter of 3.7 mm and at concentration of 0.75% did not show clear zone. Conclusion: Based on the results of the study, the lowest concentration of coriander seed extract was obtained which was able to inhibit the growth of Streptococcus mutans bacteria, is at concentration of 1.5%, the diameter of the inhibition zone of coriander seed extract had weak antibacterial activity.

Determining the Phytochemical Properties and Antibiogram of Different Chewing Stick Plants on Selected Streptococcal Species Isolated from the Oral Cavity

Chewing sticks are small twigs obtained from plant stems, measuring 12-25cm long and tied in bundles of 5-10. These twigs have been used for oral hygiene for many years, even before the invention of toothpaste, mouthwash, and mouth sprays. This study aims to determine the phytochemical properties and antibiogram of different chewing stick plants on two streptococcal species isolated from the oral cavity. Streptococcus pyogenes and Streptococcus mutans were isolated from oral swabs of patients at Rivers State University Teaching Hospital and identified to the species level. The pure isolates were further tested against the antimicrobial effects of the aqueous and ethanolic extracts of four chewing stick plants: Vernonia amygdalina, Jatropha curcas, Massularia acuminate, and Phyllanthus mullerianus. Qualitative phytochemical screening and quantitative analysis were carried out on the plant stems to determine the presence of antimicrobials. The percentage of antimicrobials present in the plant stems ranged from 33.3% to 66.7%, with Jatropha curcas, Massularia acuminate, and Vernonia amygdalina exhibiting the highest percentages, and Phyllanthus mullerianus the lowest. The antibiogram of the isolates to conventional gram-positive antibiotics was determined by the disc diffusion method. Streptococcus pyogenes showed 75% sensitivity and 25% resistance to the antibiotics, while Streptococcus mutans displayed 50% sensitivity, 16.7% intermediate, and 33.3% resistance to the antibiotics. The investigation found that the ethanolic extract of Massularia acuminate has the highest zone of inhibition of 15.5 ±0.71mm at 100mg/ml, while Phyllanthus mullerianus exhibited the least inhibition at 8 ±0mm. The ethanolic extract of Jatropha curcas and Vernonia amygdalina showed little effect on the test isolates, with a range of 0-9.5±0.71mm. The aqueous extract ranged from 8±5.7 - 9.5±0.71mm at 100mg/ml, with Massulara acuminate exhibiting the highest and the lowest zones of inhibition. This finding indicates that Massularia accuminate has the ability to suppress the growth of dental plaque-forming Streptococcus mutans and Streptococcus pyogenes. Chewing sticks are more affordable and easily accessible, so they could be recommended in community oral health programs.

UTILIZATION OF SOYBEAN POWDER AS AN ALTERNATIVE MEDIA FOR THE GROWTH OF ANAEROBIC BACTERIA

Background:Culture media or microbial growth media is a material consisting of a mixture of nutrients used by microorganisms for growth and reproduction. Anaerobic bacteria can thrive by requiring little or no oxygen for growth and metabolism. Purpose: The purpose to determine the potential of soybean powder as an alternative nitrogen source in Streptococcus mutans bacterial growth media and determine the optimum concentration of soybean powder in alternative media for Streptococcus mutans bacterial growth. Methods: The research method is a laboratory experiment to test soybean powder as an alternative medium for the growth of Streptococcus mutans bacteria using the Candle jar method. The population used is soybean powder with the brand soy flour, while the sample used is Streptococcus mutans bacteria. Results: The results obtained can be known that the average value of colony growth of Streptococcus mutans bacteria in alternative media of soybean powder with a concentration variation of 2 grams was 30 colonies, a concentration variation of 3 grams was 54 colonies, a concentration variation of 4 grams was 40 colonies, and a concentration variation of 5 grams was 72 colonies. From the average value, it can be seen that the growth of Streptococcus mutans on alternative media of soybean powder can grow well, but the most optimum growth is in the concentration variation of 5 grams. Conclusion:Based on the observations obtained, it shows that soybean powder (Glycine max (L.) Merr) can be used as an alternative medium for the growth of Streptococcus mutans bacteria.

Application of Prebiotics and Probiotics to Improve Oral and Dental Health by Inhibiting Streptococcus mutans

Background: The most common method to combat microorganisms that cause tooth decay is the use of antibiotics. However, despite the benefits of antibiotics, their use has significant disadvantages, such as the increased resistance of microorganisms to these chemicals. Objectives: A promising and safer alternative is the use of probiotics and prebiotics, which can reduce or inhibit the growth of disease-causing oral microorganisms. Streptococcus salivarius is one such probiotic, while inulin and nisin are the prebiotics commonly recommended for this purpose. Methods: Several assays, including agar bilayer interference, agar well penetration, and microdilution, were used to assess the antibacterial activities of S. salivarius K12 and S. salivarius M18 probiotics, as well as those of the prebiotics nisin and inulin. Additionally, bile salt and low pH tolerance assessments were conducted for the probiotics, and the synthesis of bacteriocins by the probiotics was verified using proteolytic and enzymatic activity assays. Results: The minimum inhibitory concentration (MIC) assay revealed that Streptococcus mutans growth was inhibited by S. salivarius K12 and S. salivarius M18 at a concentration of 75%, and by nisin and inulin at 200 IU/mL. In the well penetration assay, S. salivarius K12 exhibited significantly better results compared to S. salivarius M18. Nisin had significantly greater inhibitory effects on S. mutans compared to inulin. A combination of all four components (S. salivarius K12, S. salivarius M18, nisin, and inulin) resulted in maximum antibacterial activity, indicating a synergistic effect. Based on the agar bilayer assay and the growth inhibition zone diameters, nisin showed the highest antibacterial activity against S. mutans, followed by S. salivarius K12, inulin, and S. salivarius M18. Enzymatic digestion and proteolytic activity assays confirmed the synthesis of bacteriocins by S. salivarius K12 and S. salivarius M18. Both probiotics demonstrated bile salt tolerance at a concentration of 0.3% and surviving rates of 36% and 33%, respectively, at pH 2. Conclusions: The most important finding of the current study is the synergistic effect between the highlighted prebiotics and probiotics, which may offer a novel and effective combination for preventing tooth decay.

Assessing the antibacterial efficacy of nano propolis concentrations on Streptococcus mutans

Background: Dental caries is a common disease among the Indonesian population, with a prevalence of dental caries in Indonesia reaching 88.8%. Streptococcus mutans is one of the types of bacteria found in caries lesions. Nano propolis has antibacterial properties that can help inhibit the growth of Streptococcus mutans bacteria, reducing the risk of dental caries. Objective: This study aims to determine the effective concentration of nano propolis ranging from 2.5% to 0.625%. Methods: The research evaluates various concentrations of nano propolis, and the minimum concentration that inhibits the growth of Streptococcus mutans is observed using the colony count method. Results: The growth of Streptococcus mutans at a concentration of 2.5% nano propolis was 0, at 1.8% it was 6, at 1.25% it was 11.4, and at 0.625% it was 42. The positive control group containing BHIB media and Streptococcus mutans bacteria showed an average colony growth of 161. Conclusion: Nano propolis at a concentration of 1.25% is effective in inhibiting the growth of Streptococcus mutans.

The Test of Antibacterial Activity of Bangle Rhizome (Zingiber montanum) against the Growth of Streptococcus mutans, Porphyromonas gingivalis, and Enterococcus faecalis bacteria

The Test of Antibacterial Activity of Bangle Rhizome (Zingiber montanum) against the Growth of Streptococcus mutans, Porphyromonas gingivalis, and Enterococcus faecalis bacteria

The Antibacterial Effect of Lerak Fruit Decoction (Sapindus rarak DC) on the Growth of Streptococcus mutans as an Alternative Cavity Cleanser Material (In Vitro)

The Antibacterial Effect of Lerak Fruit Decoction (Sapindus rarak DC) on the Growth of Streptococcus mutans as an Alternative Cavity Cleanser Material (In Vitro)

Antibacterial assessment of Ziziphus mauritiana Lam on inhibition of the growth and biofilm of Streptococcus mutans

Antibacterial assessment of Ziziphus mauritiana Lam on inhibition of the growth and biofilm of Streptococcus mutans

FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF RAMBUTAN HONEY TOOTHPASTE AGAINST Streptococcus mutans

Dental caries and periodontal diseases are the most common diseases affecting the oral cavity. This can be prevented by maintaining oral hygiene. Toothpaste plays an important role in the reduction of plaque bacteria and inflammatory conditions. Rambutan honey has potential as a natural ingredient to make toothpaste with antibacterial and antioxidant properties owing to the presence of hydrogen peroxide and catalase enzymes, pH, osmolarity, and flavonoid content. The purpose of this study was to obtain a rambutan honey toothpaste formulation that effectively inhibits Streptococcus mutans growth. The laboratory experimental research method was used to combine CaCO3, glycerin, Na CMC, SLS, sodium benzoate, saccharin, menthol, and aquadest as the toothpaste base, which served as the control group. Subsequently, natural ingredients of rambutan honey at concentrations of 20%, 40%, and 60% were added to the toothpaste base to create rambutan honey toothpaste. The formulation of the rambutan honey toothpaste was followed by an antibacterial activity test using the Kirby Bauer method of disc diffusion, where the inhibition zones formed on Mueller Hinton Agar (MHA) medium were calculated. Data were analyzed using one-way and post hoc Tukey’s tests (p<0.05). The results showed that the formulation of rambutan honey toothpaste with a concentration of 60% had the greatest inhibition with 13.88±0.13 mm with a significant difference in each group (p = 0.000), so it can be concluded that rambutan honey toothpaste can inhibit the formation of Streptococcus mutans. Keywords: antibacterial, formulation, rambutan honey, toothpaste, Streptococcus mutans

Investigation of mechanical properties, remineralization, antibacterial effect, and cellular toxicity of composite orthodontic adhesive combined with silver-containing nanostructured bioactive glass

BackgroundThe formation of white spots, which represent early carious lesions, is a major issue with fixed orthodontics. The addition of remineralizing agents to orthodontic adhesives may prevent the formation of white spots. The aim of this study was to produce a composite orthodontic adhesive combined with nano-bioactive glass-silver (nBG@Ag) for bracket bonding to enamel and to investigate its cytotoxicity, antimicrobial activity, remineralization capability, and bond strength.MethodsnBG@Ag was synthesized using the sol-gel method, and characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy with an attenuated total reflectance attachment (ATR-FTIR). The cytotoxicity test (MTT) and antimicrobial activity of adhesives containing 1%, 3%, and 5% (wt/wt) nBG@Ag were evaluated, and the shear bond strength of the adhesives was measured using a universal testing machine. Remineralization was assessed through microhardness testing with a Vickers microhardness tester and scanning electron microscopy (SEM). Statistical analyses were conducted using the Shapiro-Wilk test, Levene test, one-way ANOVA, Robust-Welch test, Tukey HSD method, and two-way ANOVA.ResultsThe biocompatibility of the adhesives was found to be high, as confirmed by the lack of significant differences in the cytotoxicity between the sample and control groups. Discs made from composites containing nBG@Ag exhibited a significant reduction in the growth of Streptococcus mutans (p < 0.05), and the antibacterial activity increased with higher percentages of nBG@Ag. The shear bond strength of the adhesives decreased significantly (p < 0.001) after the addition of nanoparticles, but it remained above the recommended value. The addition of nBG@Ag showed improvement in the microhardness of the teeth, although the differences in microhardness between the study groups were not statistically significant. The formation of hydroxyapatite deposits on the tooth surface was confirmed through SEM and energy-dispersive X-ray spectroscopy (EDX).ConclusionAdding nBG@Ag to orthodontic adhesives can be an effective approach to enhance antimicrobial activity and reduce enamel demineralization around the orthodontic brackets, without compromising biocompatibility and bond strength.

THE EFFICACY OF COCOA BEAN EXTRACT MOUTHWASH CONCENTRATION IN REDUCING PLAQUE INDEX IN THE ORAL CAVITY AMONG PRIMARY SCHOOL STUDENTS AT SDN KAYEE LEU, INGIN JAYA SUBDISTRICT, ACEH BESAR

Oral and dental diseases have a high prevalence in Indonesia, with 88.8% of the population experiencing dental caries and periodontitis. Dental plaque is a soft deposit that tightly adheres to tooth surfaces and is composed of microorganisms that contribute to the development of dental caries. Preventing caries and controlling plaque can be achieved through the use of mouthwash, which is more effective when herbal mouthwash containing antioxidants and antibacterial agents is used. Cocoa bean extract (Theobroma Cacao L.) contains secondary metabolites such as alkaloids, flavonoids, tannins, steroids, and exhibits antibacterial and anti-inflammatory properties, as well as strong inhibitory effects against S. mutans bacteria. The objective of this study is to determine the effectiveness of cocoa bean extract mouthwash concentration in reducing the plaque index in the oral cavity. The research methods included laboratory experiments and quasi-experiments with a post-test only control group design. The research was conducted at the Faculty of Veterinary Medicine laboratory, University of Syiah Kuala, and SDN Kayee Leu, Aceh Besar, involving 30 students. Data analysis was performed using ANOVA in the SPSS program. The results of the study indicate that the phytochemical analysis of cocoa bean extract contains alkaloids, steroids, terpenoids, saponins, flavonoids, phenols, and tannins. The Minimum Inhibitory Concentration (MIC) of cocoa bean extract mouthwash on the growth of Streptococcus mutans was observed at a concentration of 1%, and no Minimum Bactericidal Concentration (MBC) was found in this study. The 1% and 2% concentrations of the mouthwash effectively removed plaque compared to the 0% concentration. In conclusion, the use of cocoa bean extract mouthwash is effective in reducing the plaque index. Further research should consider clinical trials to explore its potential as an herbal remedy.

TEST OF RESISTANCE OF ALPUKAT (Persea americana mill) FRUIT EXTRACT ON THE GROWTH OF THE Streptococcus mutans

Introduction: Streptococcus mutans is one of the normal flora living in the oral cavity, but in excessive amounts is the main causative agent of dental caries. The active compounds of alkaloids, saponins and quinones in avocado pulp extract is an antibacterial alternative that can inhibit the growth of Streptococcus mutans in the oral cavity. The aim of this research was to determine the inhibitory power that contain in avocado pulp against Streptococcus mutans . Materials and Methods: This study used 4 concentrations namely 25%, 50%, 75% and 100% with each repetition 4 times. Avocado pulp extraction was carried out by maceration method using 96% ethanol solvent. Inhibition zone testing was carried out using the Kirby baurer method. Results and Discussions: The zone of inhibition was determined using the Kruskal wallis test, which showed that there were significant differences in various concentrations against the growth of Streptococcus mutans bacteria. Avocado fruit pulp extract is able to inhibit the growth of Streptococcus mutans bacteria because avocado fruit pulp extract has active compounds that cause antibacterial activity. Active compounds of avocado fruit flesh that are efficacious as antibacterials are alkaloids, saponins and quinones. Conclusion: Avocado pulp extract has been proven to have an antibacterial effect against Streptococcus mutans with an effective concentration level of 100%. Increasing the concentration of avocado pulp extract was also shown to affect the diameter of Streptococcus mutans inhibition zone

Antibacterial Efficacy of Herbal and 0.2% Chlorhexidine Mouth Rinse Against Oral Pathogens – An In vitro Study

ABSTRACT Background: Nature has given us an enormous number of medicines for every disease that affects people. Aim: To compare the antimicrobial efficacy of an herbal and 0.2% chlorhexidine gluconate mouthrinse against Streptococcus mutans, Staphylococcus aureus, and Enterococcus faecalis. Materials and Methods: The antimicrobial effectiveness (zone of inhibition) of a herbal mouthrinse and 0.2% chlorhexidine mouthrinse were determined by agar well-diffusion method. Results: At 50% concentration, the experimental mouthrinse inhibits the growth of Streptococcus mutans, Staphylococcus aureus, and Enterococcus faecalis. Conclusion: Against the three strains, chlorhexidine mouthrinse (0.2%) performs better in terms of antimicrobial effectiveness.

DAYA HAMBAT DARI EKSTRAK SARANG BURUNG WALET PADA PERTUMBUHAN BAKTERI STREPTOCOCCUS MUTANS DAN PORPHYROMONAS GINGIVALIS

Type 2 diabetes (T2DM) is one of the fastest growing global diseases of the 21st century, with its prevalence more than tripling in the last 20 years and affecting 10.5% of the world's population aged 20-79 years. In Indonesia, diabetes mellitus among 20-79 year olds is 10.6%. The most widely cultivated type of swallow nest is Collocatia fuciphaga. Collocatia fuciphaga is a species of bird that produces nests from its white saliva. Swallow's nest contains sialic acid which is only produced from saliva, calcium, glycoproteins and Epidermal Growth Factor (EGF), one of the benefits of which is to accelerate wound healing. The study aimed to determine the inhibition of swallow's nest extract on the growth of Streptococcus mutans and Porphyromonas gingivalis bacteria. The swallow's nest extract test used an experimental design approach, namely conducting laboratory tests. The initial stage was to make swallow's nest extract by maceration technique using 96% ethanol solvent (the aim is to get anti-bacterial active substances, namely D-Galactose and Sialic acid contained in swallow's nest), the result of the extraction was a dry extract weighing 1.8195 grams which was then dissolved again using DMSO solution with concentrations of 10%, 20%, and 30%. The second stage is the swallow's nest extract test with the two test bacteria. The results of the swallow's nest extract test at concentrations of 10%, 20%, 30% against Streptococcus mutans and Porphyromonas gingivalis bacteria showed no inhibition. Future research could be further developed toward wound healing

ANTIBACTERIAL EFFECTIVENESS OF RAMBAI (Sonneratia Caseolaris) LEAVES EXTRACT AGAINST STREPTOCOCCUS MUTANS

Background: The 2018 Riskesdas data stated that dental caries prevalence in Indonesia was 88.8%. Dental caries is a multifactorial disease characterized by hard tissue damage to the enamel, dentin, and cementum of the teeth. One of the causes of caries is the presence of a microorganism called Streptococcus mutans. Rambai leaf extract (Sonneratia caseolaris) is a typical plant of South Kalimantan with content flavonoids, tripernoids, tannins, phenols, steroids that have antibacterial properties against the growth of Streptococcus mutans which can prevent caries. Purpose: To determine the difference in the effectiveness of the antibacterial extract of rambai leaves (Sonneratia caseolaris) against bacteria Streptococcus mutans in vitro. Methods: This research was a true experimental with a post-test-only design and a control group, using six treatment groups, namely 20%, 40%, 60%, 80%, positive control (povidone iodine 1%), and negative control (aquadest) respectively. Each treatment got 3 repetitions. The antibacterial test method used in this study was the liquid dilution method to determine the Minimun Inhibitory Concentration (MIC) by using Spectrophotometer and solid dilution to determine the Minimum Bactericidal Concentration (MBC) by using tools Colony Counter. Results: Based on the results and data analysis, it can be seen that the rambai leaf extract has a MIC of 20% and a MBC of 60%. Conclusion: Rambai leaf extract (Sonneratia caseolaris) can inhibit and have antibacterial activity against the growth of Streptococcus mutans. Keywords: Antibacterial, Rambai leaf extract, Streptococcus mutans

Potential of 10% propolis-based toothpaste on the inhibition of biofilm-forming bacteria growth in vitro

Aim of the study: To investigate the potential of 10% propolis-based toothpaste on inhibiting biofilm-forming bacteria growth in vitro. Material and method: Organoleptic properties are evaluated, considering color, odor, and taste. Antibacterial tests use a disc diffusion method against Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria, while cytotoxicity is assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay on fibroblast cells. Statistical analysis involves mean ± standard deviation. The data were then tested using a one-way analysis of variance and Kruskal-Wallis, followed by post-hoc test (p &amp;lt; 0.05). Results: The organoleptic evaluation of 10% propolis toothpaste reveals a visually clear appearance, consistent orange flavor, and aroma lasting 30 days. Based on the antibacterial results, a 10% level of propolis toothpaste sample inhibited the growth of Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria. The post-hoc test showed that toothpaste demonstrated significant inhibition on S. mutans and S. aureus compared to the negative control (p &amp;lt; 0.05). The toothpaste showed a larger inhibitory zone towards P. gingivalis compared to the adverse control; however, no significant differences were observed (p &amp;gt; 0.05). Cytotoxicity assessment on fibroblast cells shows a high percentage (85.31%) of viable cells. The findings highlight the 10% of propolis toothpaste’s potential and non-toxic as oral care product. Conclusions: 10% propolis toothpaste inhibits S. mutans, S. aureus, P. gingivalis growth, and not toxic on fibroblast.

Antibacterial Activity and Shear Bond Strength of Orthodontic Adhesive Containing Various Sizes of Chitosan Nanoparticles: An In Vitro Study.

White spot lesions are common after orthodontic treatment. Chitosan nanoparticles (CS-NPs) have emerged as promising antibacterial agents that inhibit the growth of Streptococcus mutans. The aim of the study was to investigate the nano-effect of adhesives containing CS-NPs on S. mutansand their effects on shear bond strength. The inhibitory effects of two sizes of CS-NPs were assessed using the disc agar diffusion method. Four wells were created in the petri dishes, and each was inoculated with broth (negative control), chlorhexidine (positive control), CS-NPs (20 nm), or CS-NPs (131 nm). An Instron machine was used to evaluate shear bond strength by allocating 24 teeth into three groups, and all measurements were recorded in megapascals. Caries progression was assessed using the International Caries Detection and Assessment System and surface profilometry. Statistical analysis was performed using IBM SPSS Statistics for Windows, Version 27.0 (Released 2020; IBMCorp., Armonk, New York, United States) for a one-way ANOVA followed by Tukey's multiple comparison test. Disc agar diffusion showed a reduction in S. mutans in the CS-NPgroup compared to the control (p < 0.001), with no statistical significance between the sizes of 20 and 131 nm (p = 0.95). Regarding shear bond strength, no differences were recorded when adhesive-containing CS-NPs and the control were compared (p = 0.44). Additionally, no differences were found within the CS-NP groups (p = 0.91). Caries assessments showed excellent agreement, as indicated by a weighted kappa. Profilometry readings showed higher surface roughness in the control than in the CS-NP groups (p < 0.001), with no statistically significant difference between the CS-NP groups (p = 0.72). CS-NPs of both sizes tested had similar antibacterial effects. In addition, the incorporation of CS-NPs did not affect shear bond strength.