The potential honey supply available in the Republic of Uzbekistan can have up to 2 million bee families. For the development of the beekeeping industry in Uzbekistan, it is necessary to create a purebred population of Apis mellifera carpatica, based on the reproduction of early purebred queen bees. In addition to protein fi llers and mineral complexes, probiotics were used as part of stimulating feed additives for autumn and spring stimulation of egg production of queen bees. However, in our opinion it is better to add prebiotics to the composition of feed additives, which are available in the composition of infant formula used for baby food. It has been established by studies that stimulating feed additives with protein fi llers, combined with cobalt sulfate and a mixture of “Nanny 2 with prebiotic” positively infl uence on the growth, development and economically useful traits of maternal, paternal and queen-rearing colonies in preparation for winter. It has been proved that a high rate of growth of the brood being grown is carried out when giving honey satiety in the composite form with CoSO4 + “Nanny 2 with prebiotic” mixture. In comparison with the background level, this parameter reaches its maximum by the third accounting, increasing in maternal families by 2,41 times, in paternal families – 2,61 times, in queen-rearing colonies – 2,61 times. It has been found that the Carpathian queen bees showed their high genetic capabilities for egg production during the autumn increase in the strength of families with the 3rd and 4th variants of feed additives until the end of the experiment. The maximum rate of egg laying females recorded in the 4th group, exceeded the same level of the 1st group of the parent families by 1,44 times, 2nd group – 1,24 times 3rd group by 1,14 times. An adequate pattern has been registered for paternal and queen-rearing colonies. Stimulating feed additives in particular, the use in group 4 of honey additive in a composite form with CoSO4 + mixture of “Nanny 2 with prebiotic” has a prolonging eff ect, both on the egg-laying ability of queen bees, and on brood feeding, which increases the strength of bee families. By the beginning of winter, this force was 15,4 and 16,0 seams of bees between two combs in the maternal families, 15,5 and 15,8 seams of bees between two combs in the paternal families, and 15,8 and 16,0 seam of bees between two combs in the queen-rearing colonies, respectively. This indicates that a complete generation of autumn bees will be obtained, which will not only successfully overwinter, but will also be actively used in the breeding of extra early queen bees in the new season.