Stream Invertebrates Research Articles

Disproportional vulnerability of mountain aquatic invertebrates to climate change effects

ABSTRACT Mountain freshwater communities are generally considered sensible to accelerated climatic changes, though their vulnerabilities have not been well evaluated. Individual species or species groups are expected to respond differently depending on their adaptations, traits, or distributions, but this has not yet been distinguished. This work used available climate change vulnerability scores (ccvs) of European Ephemeroptera, Plecoptera, and Trichoptera (EPT) species (n = 1,402) to (1) compare the vulnerability between species pools of different ecoregions including alpine species and alpine endemics, (2) contrast the vulnerability between the different insect orders, and (3) assess the altitude–vulnerability relationship within the European Alps. We revealed fifty alpine Plecoptera and Trichoptera species that are categorized highly vulnerable to climate change effects (= 31 percent of all highly vulnerable European species) with the highest proportions in species inventories of alpine endemics and high-altitude waters (51 percent of high-altitude species are classified as highly vulnerable). The ccvs analysis specifically for mountain waters shows that a disproportionately high number of alpine species, and particularly alpine endemics, will be affected by climate change and suggests that Ephemeroptera may be better prepared than Plecoptera and Trichoptera. Thus, this trait-based evaluation suggests that mountain stream invertebrate communities are undergoing disproportionate restructurings in response to climate change effects more than lowland communities are.

Taxonomic identity, biodiversity, and antecedent disturbances shape the dimensional stability of stream invertebrates

AbstractThe “dimensional stability” approach measures different components of ecological stability to investigate how they are related. Yet, most empirical work has used small‐scale and short‐term experimental manipulations. Here, we apply this framework to a long‐term observational dataset of stream macroinvertebrates sampled between the winter flooding and summer monsoon seasons. We test hypotheses that relate variation among stability metrics across different taxa, the magnitude of antecedent (monsoon) and immediate (winter) floods to stability metrics, and the relative importance of disturbance magnitude and taxonomic richness on community dimensional stability. Cluster analysis revealed four distinct stability types, and we found that the magnitude of floods during the prior monsoon was more important in influencing stability than the winter flood itself. For dimensional stability at the community level, taxonomic richness was more important than disturbance magnitude. This work demonstrates that abiotic and biotic factors determine dimensional stability in a natural ecosystem.

Bucketloads of aquatic invertebrates in a dry intermittent stream.

Bucketloads of aquatic invertebrates in a dry intermittent stream.

Biomonitoring using stream invertebrates in Kenya: A review of status, challenges and future prospects

Biomonitoring using stream invertebrates in Kenya: A review of status, challenges and future prospects

Plasticity of upper thermal limits of Australian Paratya spp. (Decapoda, Atyidae) and considerations of climate-change adaptation

Context The ability of ectothermic stream invertebrates to adapt to the predicted increases in mean and extreme stream temperatures is crucial to ensuring they continue to exist. Aims To examine the plasticity of thermal limits of Australian Paratya spp. (Decapoda, Atyidae) from streams in eastern New South Wales (NSW). We hypothesised that the upper lethal temperature (ULT, as indicated by the median lethal temperature, LT50) would be higher for warm water-acclimated shrimp individuals than for winter-acclimatised shrimp individuals because of the importance of acclimatisation temperature. Methods Controlled experiments were undertaken to determine the ULT by using ramping assays for winter field-acclimatised and warm water laboratory-acclimated Paratya spp. Key results Warm water-acclimated shrimp individuals demonstrated a significantly higher LT50 of 36.1°C than did winter-acclimatised shrimp individuals at 34.6°C. Paratya spp. exhibited a limited plasticity for acclimation to warmer temperatures. Conclusions Results demonstrated the potential vulnerability of ectothermic stream invertebrates to climate change if stream temperatures increase as predicted and thermal thresholds are exceeded. Implications Understanding the ULT of stream invertebrates helps predict their ability to respond to temperature variability and response to climate change. Increasing resilience through target management of resorting riparian vegetation for shade and securing environmental flows may reduce the impacts of stream warming.

Tertiary wastewater treatment combined with high dilution rates fails to eliminate impacts on receiving stream invertebrate assemblages

The amount of wastewater processed in treatment plants is increasing following more strict environmental regulations. Treatment facilities are implementing upgrades to abate the concentrations of nutrients and contaminants and, thus, reduce their effects on receiving systems. Although many studies characterized the chemical composition and ecotoxicological effects of treated wastewater, its environmental effects are still poorly known, as receiving water bodies are often subjected to other stressors. We performed a field manipulative experiment to measure the response of invertebrate assemblages to one year of tertiary-treated wastewater discharges. We poured treated wastewater from an urban wastewater treatment plant into the lower-most 100-m of a previously unpolluted stream (3.6 % daily flow on average) while using another upstream reach as control. The positive correlation between effect sizes of abundance changes and IBMWP scores suggested assemblage modifications were following taxa tolerance to ecological impairment. The treatment increased the temporal variability of SPEARorganic, EPT relative abundance, and invertebrate functional redundancy. Our results show that even in this best-case scenario of tertiary-treated and highly diluted wastewater, the abundance of the most sensitive taxa in the aquatic assemblages is reduced. Further improvements in wastewater treatments seem necessary to ensure these effluents do not modify receiving water ecosystems.

Use of Aquatic Insects to Assess the Biological Status of a Perennial Pond in Assam, Northeast India

An investigation was carried out on “Hazara Pukhuri”, a perennial pond in Sonitpur district, North East India, between July 2019 and June 2020. To assess the health of the waterbody, various biotic and diversity indices were applied, with aquatic and semi-aquatic hemipteran populations functioning as bioindicators. The pond’s hemipterans include 17 species from 13 genera and 8 families, including Gerridae, Corixidae, Pleidae, Notonectidae, Nepidae, Belostomatidae, Hydrometridae, and Mesoveliidae. The biotic indices Average Score per Taxon (ASPT), Stream Invertebrate Grade Number-Average Level (SIGNAL-2), and The Biological Monitoring Working Party (BMWP) Score, as well as other diversity indices, were assessed to indicate that the waterbody was unpolluted/ slightly polluted. The presence or lack of littoral vegetation and flooding and drying of nearby shallow water pools and swampy areas were important drivers of the distribution, abundance, and community composition of aquatic and semi-aquatic hemipterans in the studied water body.

Effects of riparian forest and agricultural land use on stream fish diet and trophic position

AbstractTo examine agricultural impacts on stream food webs, we used gut content analysis (GCA) and stable isotope analysis (SIA) to investigate the influence of riparian forest buffers on the diet and trophic position of five stream fish species in a row‐crop agricultural watershed. Six study streams were divided into two categories (forested and agricultural) based on percentages of riparian forest and agriculture in the watershed. Results suggest alterations in riparian vegetation (forested vs. cropland) had strong impacts on instream characteristics and basal resources that affected prey abundances and fish trophic structure. Although differences varied among species, GCA revealed fish in forested streams had a more diverse diet that included greater numbers of terrestrial invertebrates and aquatic invertebrate predators, whereas fish in agricultural streams consumed more aquatic herbivores and decapods. Diet shifts of the omnivorous creek chub followed differences in basal resource biomass, with greater proportions of algae consumed in agricultural sites contrasted by greater detritus consumed in forested sites. SIA Bayesian mixing‐model results supported diet differences shown by GCA, including higher contributions of terrestrial invertebrates to fish diets in forested streams. However, the isotopic overlap of aquatic invertebrates in agricultural streams limited diet contribution comparisons. SIA indicated fish trophic position was higher in forested than agricultural streams for all species, but GCA was required to identify that greater consumption of predatory prey likely caused this widespread change in fish trophic structure. Results highlight the benefits of integrating SIA and GCA and the importance of riparian forests to stream fishes in agricultural watersheds.

The Wild and Wonderful World of Stream Bugs

Under the surface of most streams a strange world exists, made up of hundreds of small critters called aquatic invertebrates. “Aquatic” means they live in the water and “invertebrate” means they have no backbones or even skeletons. Some have body parts that look like they came from an alien planet. Some have slippery bodies adapted to rushing stream water. These organisms play many important roles. Some live for up to 7 years and others live for as little as 2 weeks. Some eat slime growing on stream rocks and others eat leaves. Some, like the golden stonefly, even stalk and eat other invertebrates, like a tiger in a jungle. In national parks and around the world, scientists use these insects, worms, leeches, and mites to tell if the water is healthy. We can do this because certain stream invertebrates disappear if water is polluted, or if the stream habitat is degraded.

On the delay between water temperature and invertebrate community response to warming climate.

We evaluated the effect of global warming on invertebrate communities at high altitudes using data from the Careser system. We procured data on air temperature, which was obtained over 50 years at altitudes above 2600 m a.s.l., and data on water temperature, which was available for approximately 30 years. We sampled thrice in the past 20 years (2001, 2014, 2018) at three sampling sites (CR0-metakryal, CR1-hypokryal, CR2-glacio-rhithral) of the Careser glacier-fed stream and its main non-glacial tributary (CR1bis-krenal). Warmer climates were observed in the last decade compared to the 1980s, with a mean maximum summer air temperature (mTmax) increase of 1.7 °C at 2642 m a.s.l. and 1.8 °C at 2858 m a.s.l. Compared to air temperatures, the rise in water temperature was delayed by approximately 20 years; water mTmax started to increase in 2003, reaching 8.1 °C at 2642 m a.s.l. and 2.4 °C at 2858 m a.s.l in the year 2020. The invertebrate community exhibited a delayed response approximately 13 years from the water warming; there was a sequential increase in the number of taxa, Shannon diversity, and after 17 years, functional diversity. In the kryal sites, taxonomical and functional diversity changed more consistently than in the glacio-rhithral site in the same period, due to the arrival of taxa that were previously absent upstream and bearers of entirely new traits. Progressive taxonomical homogenisation was evident with decreasing glacial influence, mainly between glacio-rhithral and krenal sites. The numbers of Diamesa steinboecki, an insect that was adapted to the cold, declined in summer (water mTmax >6 °C and air mTmax >12 °C). This study highlights the mode and time of response of stream invertebrate communities to global warming in alpine streams and provides guidelines for analysing changes in the stream invertebrate communities of other glacial systems in alpine regions.

Assessment of the stream invertebrate -diversity along an elevation gradient using a bidimensional null model analysis.

β‐Diversity, commonly defined as the compositional variation among localities that links local diversity (α‐diversity) and regional diversity (γ‐diversity), can arise from two different ecological phenomena, namely the spatial species turnover (i.e., species replacement) and the nestedness of assemblages (i.e., species loss). However, any assessment that does not account for stochasticity in community assembly could be biased and misinform conservation management. In this study, we aimed to provide a better understanding of the overall ecological phenomena underlying stream β‐diversity along elevation gradients and to contribute to the rich debate on null model approaches to identify nonrandom patterns in the distribution of taxa. Based on presence‐absence data of 78 stream invertebrate families from 309 sites located in the Swiss Alpine region, we analyzed the effect size of nonrandom spatial distribution of stream invertebrates on the β‐diversity and its two components (i.e., turnover and nestedness). We used a modeling framework that allows exploring the complete range of existing algorithms used in null model analysis and assessing how distribution patterns vary according to an array of possible ecological assumptions. Overall, the turnover of stream invertebrates and the nestedness of assemblages were significantly lower and higher, respectively, than the ones expected by chance. This pattern increased with elevation, and the consistent trend observed along the altitudinal gradient, even in the most conservative analysis, strengthened our findings. Our study suggests that deterministic distribution of stream invertebrates in the Swiss Alpine region is significantly driven by differential dispersal capacity and environmental stress gradients. As long as the ecological assumptions for constructing the null models and their implications are acknowledged, we believe that they still represent useful tools to measure the effect size of nonrandom spatial distribution of taxa on β‐diversity.

Structure and functional composition of macroinvertebrate communities in coastal plain streams across a precipitation gradient

Abstract Climate change is expected to alter rainfall and temperature regimes across the world. The hydrology and riparian zone vegetation of lotic ecosystems are tightly linked to rainfall and a mechanistic understanding of the effects of rainfall on lotic ecosystems is needed to forecast the ecological impacts of climate change. However, it is difficult to isolate rainfall effects from other environmental variables that covary across climates. To address this, we leveraged a unique steep rainfall gradient with few covarying changes in elevation, temperature, and geology to evaluate the effects of rainfall on stream invertebrate communities. We surveyed nine streams in the Texas Gulf Coast Prairie distributed along a 550–1,350 mm/year rainfall gradient. Four sites were classified as drier semi‐arid streams (<750 mm annual rainfall) and five sites were classified as wetter sub‐humid streams (>750 mm annual rainfall). A suite of characteristics including benthic invertebrate community metrics, flow conditions, and water quality variables were assessed monthly for 14 months at each site to relate precipitation regime to stream structure and function. Precipitation regime was observed to be a master explanatory variable. As annual rainfall increased, the flow environment became more stable within seasons and predictable across seasons, influencing spatial structure and temporal variability of invertebrate community composition. Wetter streams were dominated by slower growing taxa without adaptions for desiccation resistance and strong dispersal. Wetter sites displayed seasonal variation in community composition and species richness, whereas temporal variation in communities in drier streams was controlled by stochastic variation in flow conditions. These observations show that differences in local annual rainfall correlated with major changes to community structure and functional composition. We hypothesise that this association is related to the connection of rainfall to hydrological stability, particularly the frequency of low flow disturbances, and the subsequent effects on riparian vegetation and temporally available niches to stream invertebrates. Our work adds to evidence that alterations in precipitation patterns associated with climate change have sweeping impacts on lotic fauna.

Rethinking biodiversity patterns and processes in stream ecosystems

AbstractA major goal of community ecology is understanding the processes responsible for generating biodiversity patterns along spatial and environmental gradients. In stream ecosystems, system‐specific conceptual frameworks have dominated research describing biodiversity change along longitudinal gradients of river networks. However, support for these conceptual frameworks has been mixed, mainly applicable to specific stream ecosystems and biomes, and these frameworks have placed less emphasis on general mechanisms driving biodiversity patterns. Rethinking biodiversity patterns and processes in stream ecosystems with a focus on the overarching mechanisms common across ecosystems will provide a more holistic understanding of why biodiversity patterns vary along river networks. In this study, we apply the theory of ecological communities (TEC) conceptual framework to stream ecosystems to focus explicitly on the core ecological processes structuring communities: dispersal, speciation, niche selection, and ecological drift. Using a unique case study from high‐elevation networks of connected lakes and streams, we sampled stream invertebrate communities in the Sierra Nevada, California, USA to test established stream ecology frameworks and compared them with the TEC framework. Local diversity increased and β‐diversity decreased moving downstream from the headwaters, consistent with the river continuum concept and the small but mighty framework of mountain stream biodiversity. Local diversity was also structured by distance below upstream lakes, where diversity increased with distance below upstream lakes, in support of the serial discontinuity concept. Despite some support for the biodiversity patterns predicted from the stream ecology frameworks, no single framework was fully supported, suggesting “context dependence.” By framing our results under the TEC, we found that species diversity was structured by niche selection, where local diversity was highest in environmentally favorable sites. Local diversity was also highest in sites with small community sizes, countering the predicted effects of ecological drift. Moreover, higher β‐diversity in the headwaters was influenced by dispersal and niche selection, where environmentally harsh and spatially isolated sites exhibit higher community variation. Taken together our results suggest that combining system‐specific ecological frameworks with the TEC provides a powerful approach for inferring the mechanisms driving biodiversity patterns and provides a path toward generalization of biodiversity research across ecosystems.

Impacts of urbanization on chloride and stream invertebrates: A 10-year citizen science field study of road salt in stormwater runoff.

The use of deicing agents during the winter months is one of many stressors that impact stream ecosystems in urban and urbanizing watersheds. In this study, a long-term data set collected by citizen scientists with the Missouri Stream Team was used to evaluate the relationships between watershed urbanization metrics and chloride metrics. Further, these data were used to explore the effects of elevated chloride concentrations on stream invertebrate communities using quantile regression. While the amount of road surface in a watershed was a dominant factor in predicting the maximum chloride measurement, the median chloride concentration was also strongly related to the amount of medium-to-high density development in the watershed, suggesting that nonmunicipal salt use is an important contributor to increases in base flow chloride concentrations. Additionally, chloride concentration appears to be one of the many factors that impact invertebrate density and diversity measurements, with decreases in invertebrate diversity corresponding with the US Environmental Protection Agency water quality criteria. Our findings suggest that the use of chloride-based road salt on municipal roads as well as on nonmunicipal settings is contributing to a loss of diversity and density of aquatic invertebrate communities in urban regions. Integr Environ Assess Manag 2022;18:1667-1677. © 2022 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Metabolic plasticity can amplify ecosystem responses to global warming

Organisms have the capacity to alter their physiological response to warming through acclimation or adaptation, but the consequence of this metabolic plasticity for energy flow through food webs is currently unknown, and a generalisable framework does not exist for modelling its ecosystem-level effects. Here, using temperature-controlled experiments on stream invertebrates from a natural thermal gradient, we show that the ability of organisms to raise their metabolic rate following chronic exposure to warming decreases with increasing body size. Chronic exposure to higher temperatures also increases the acute thermal sensitivity of whole-organismal metabolic rate, independent of body size. A mathematical model parameterised with these findings shows that metabolic plasticity could account for 60% higher ecosystem energy flux with just +2 °C of warming than a traditional model based on ecological metabolic theory. This could explain why long-term warming amplifies ecosystem respiration rates through time in recent mesocosm experiments, and highlights the need to embed metabolic plasticity in predictive models of global warming impacts on ecosystems.

Flow amplitude or up‐ramping rate? Quantifying single and combined effects on macroinvertebrate drift during hydropeaking simulations, considering sensitive traits

AbstractThe hydrological regime of many alpine rivers is heavily altered due hydroelectric power generation. Hydropeaking operation produces frequent and irregular discharge fluctuations. Depending on the operational changes of flow amplitude and/or up‐ramping rate as well as on river morphology, hydropeaking can lead to quick and strong variations in hydraulic stress affecting stream invertebrates and causing increased drift. In the present flume experimental study, we analyzed trait‐specific drift reactions to single and combined effects of increased flow amplitude and up‐ramping rate. We analyzed taxa according to their hydraulic habitat preference and flow exposure, as these traits seem to be indicative toward hydropeaking. The results show that the sudden increase in discharge and related flow velocity led to increased macroinvertebrate drift proportions in hydropeaking treatments, which differed significantly to parallel control runs (mean drift proportion in all hydropeaking setups: 13% compared to 5% in controls). Increasing flow amplitudes led to an increase in drift for most taxa and traits. This was particularly significant for taxa associated with lentic areas. The effect of the up‐ramping rate on macroinvertebrate drift was nonsignificant but showed strong interactive effects with the flow amplitude, especially for taxa dwelling on the substrate surface. Our results therefore indicate that discharge‐related parameters, such as flow velocity, primarily affect macroinvertebrate drift and the importance of the up‐ramping rate increases, if certain discharge‐related thresholds are exceeded. Vice versa, a reduction of the up‐ramping rate at hydropeaking events with high flow amplitudes may reduce the effect on macroinvertebrate drift. Flow‐exposed (surface) and flow‐sensitive (lentic) taxa showed distinct drift reactions following hydropeaking treatments, which were significantly higher compared to effects on taxa associated to lotic and interstital habitats. Therefore, we conclude that both traits (hydraulic and vertical habitat preference) have proven as promising for analyzing hydropeaking effects. The trait classifications should be extended to a higher number of taxa and to different life stages as these may show different drift patterns.

The terrestrial and semi-aquatic invertebrates of intermittent rivers and ephemeral streams.

ABSTRACTIntermittent rivers and ephemeral streams (IRES), which cease flow and/or dry at some point, are the most abundant waterways on earth, and are found on every continent. They can support a diverse, and often abundant, terrestrial and semi‐aquatic invertebrate (TSAI) fauna, which has been poorly explored due to its position at the fringe between aquatic and terrestrial disciplines. TSAIs can inhabit a variety of habitat types, including the shoreline, the surface of exposed gravel bars, unsaturated gravels, dry riverbeds, riparian zones, and floodplains. Much less is known about the species composition and ecological roles of TSAIs of IRES than their aquatic counterparts, with TSAIs being largely overlooked in conceptual models, legislation, policy, and ecological monitoring. Herein we review the TSAI literature that has increased substantially over the last decade and present conceptual models describing how TSAIs respond to hydrological changes in IRES. Then, we test these models with data collected during wet and dry phases in IRES from Australia and France. These generic models can be utilised by water managers and policy makers, ensuring that both wet and dry phases are considered in the management and protection of IRES. IRES should be viewed as a habitat continuum through time, with taxa from a pool of aquatic, semi‐aquatic and terrestrial invertebrates inhabiting at any hydrological stage. We call for collaboration among terrestrial and aquatic ecologists to explore these invertebrates and ecosystems further.

Macroinvertebrate responses to differing riparian treatments following forest harvest in the headwaters of Trask River watershed

Although it is well-established that macroinvertebrates are an integral part of a healthy stream ecosystem, studies designed to inform forest harvest best management practices for sustainability of macroinvertebrate communities in small streams have been limited. Current riparian management practices for headwater streams generally focus on providing adequate wood supply, reducing debris flows and transport of sediment, and maintaining water quality in larger streams that contain fish. Here we studied the effects of contemporary forest harvest practices on benthic macroinvertebrate density and community composition in 12 small headwater streams as part of the Trask River Watershed Study. To account for seasonal and year-to-year variability in macroinvertebrate densities, we sampled each site at the same time of year for six years before and four years after harvest. Three watersheds were clearcut with variable riparian buffers, three were clearcut with 12 m riparian buffers, and one was thinned with 15 m buffers. Five adjacent watersheds were not treated and were studied as reference sites. All sites had approximately 50 yr old conifer and alder forests, which had revegetated following fires and harvest in prior decades.In these steep mountain streams, benthic macroinvertebrate density and community composition were generally similar across watersheds prior to harvest. Collectors were the most abundant functional feeding group, followed by shredders; scrapers were the least abundant. After harvest, in two of the three clearcut watersheds with variable buffers, the total benthic density and percentage of collectors increased, primarily due to greater numbers of Chironomidae. We also observed more abundant emergence post-harvest at these two watersheds. In the third clearcut watershed with a variable buffer and in the three clearcut watersheds with uniform 12 m riparian buffers, we did not see changes in density or functional feeding groups after harvest. We explored the spatial and temporal aspects of the data using both non-parametric and parametric statistical methods. High variability swamped the treatment effect when watersheds were grouped by riparian treatment for the repeated measure analyses, while NMDS showed a significant shift post-harvest for the watersheds with variable buffers. In the clearcut watersheds with uniform buffers, macroinvertebrates densities and community composition did not shift. At two of the clearcut watersheds with variable buffers, we observed increased density for the first two years after harvest, and chironomids comprised up to 80% of the abundance in samples. In the subsequent years, we documented mixed responses for two watersheds. It was invaluable to have multiple pre- and post-harvest sampling periods in both harvested and reference watersheds in order to better identify site specific responses to treatment and to separate background variability from responses to harvest. We show that retention of riparian vegetation on headwater streams during clearcut harvesting minimized changes in total macroinvertebrate densities and community composition. Small buffers may be key to maintenance of diverse stream invertebrate communities in headwaters.

Exploring the interplay of biotic interactions and salinity stress in freshwater invertebrate assemblages: a response to Kefford

Controlled mesocosm experiments can add substantially to our knowledge of the influence of environmental factors on freshwater assemblages by partitioning the possible effects of different drivers. Reporting results of such an experiment, Bray et al. (2019) concluded that effects of salinity on salt-sensitive stream invertebrates were substantially modified by interspecific biotic interactions with salt-tolerant invertebrates from a high-salinity stream. Chessman (2021) questioned this conclusion on three grounds: (1) confounding of the experimental design, (2) lack of evidence that purported diverse effects of biotic interactions were beyond mere stochastic variation, and (3) absence of mechanistic explanations for supposed effects grounded in organism biology and ecology. Chessman (2021) also conducted an independent statistical analysis of publicly available data from the experiment, which did not support the study’s conclusions. Kefford et al. (2022) dispute Chessman’s (2021) findings by analysing previously unpublished data from the experiment, which they claim demonstrates that the experimental design was not confounded, and criticise Chessman’s (2021) statistical analysis. Here, I respond to their new analysis and criticisms, explaining why they do not dispel any of the concerns expressed by Chessman (2021).

A Bayesian Belief Network learning tool integrates multi-scale effects of riparian buffers on stream invertebrates

Riparian forest buffers have multiple benefits for biodiversity and ecosystem services in both freshwater and terrestrial habitats but are rarely implemented in water ecosystem management, partly reflecting the lack of information on the effectiveness of this measure. In this context, social learning is valuable to inform stakeholders of the efficacy of riparian vegetation in mitigating stream degradation. We aim to develop a Bayesian belief network (BBN) model for application as a learning tool to simulate and assess the reach- and segment-scale effects of riparian vegetation properties and land use on instream invertebrates. We surveyed reach-scale riparian conditions, extracted segment-scale riparian and subcatchment land use information from geographic information system data, and collected macroinvertebrate samples from four catchments in Europe (Belgium, Norway, Romania, and Sweden). We modelled the ecological condition based on the Average Score Per Taxon (ASPT) index, a macroinvertebrate-based index widely used in European bioassessment, as a function of different riparian variables using the BBN modelling approach. The results of the model simulations provided insights into the usefulness of riparian vegetation attributes in enhancing the ecological condition, with reach-scale riparian vegetation quality associated with the strongest improvements in ecological status. Specifically, reach-scale buffer vegetation of score 3 (i.e. moderate quality) generally results in the highest probability of a good ASPT score (99–100%). In contrast, a site with a narrow width of riparian trees and a small area of trees with reach-scale buffer vegetation of score 1 (i.e. low quality) predicts a high probability of a bad ASPT score (74%). The strengths of the BBN model are the ease of interpretation, fast simulation, ability to explicitly indicate uncertainty in model outcomes, and interactivity. These merits point to the potential use of the BBN model in workshop activities to stimulate key learning processes that help inform the management of riparian zones.