ABSTRACT The Czech Republic is one of the several countries in the world and the only unitary democratic state allowing regions to initiate legislation without limits. Despite this, a study of Czech regional initiatives has yet to be written. Therefore, this paper delivers a comprehensive analysis of the phenomenon. The results show that the activity and success rates of the regions differ substantially. The probability of a regional proposal is positively affected by the region’s legislative strength in the Chamber of Deputies and the opposition status of the region’s governor. In contrast, it is negatively affected by the distance from Prague. The chances of adopting the regional initiative substantially decrease if a proposal suggests a shared rule instrument compared to self-rule ones. Overall, the article argues that while regional legislative activity in (con)federal states is affected mainly by regionalist strategic politics, the process in the unitary state is more result-oriented.