Strains Of Hens Research Articles

Effect of Different Proportions of Probiotics on Immunological Blood Parameters of Babcock Laying Hens Strain

The research was conducted at the Research Center of Agriculture Faculty of the University of Aleppo. 240 chickens of Babcock B300 strain were used and distributed randomly from day one into four independent groups. Each group included 60 birds distributed into three replicates (20 birds). The control group (A group) was fed a balanced standard feed mixture (1-18 weeks) without additives, while lactic acid bacillus was added to treatments groups according to three levels (50 mg/kg feed; B group, 100 mg/kg feed; C group and 150 mg/kg feed; D group). Haematological analyzes were performed over two- time intervals (1-21 weeks and 22-32 weeks). In the first time interval, results showed that a rise in blood albumin (P<0.001) was also observed in C and D groups compared to the A group. The rates of blood globulin increased (P<0.02) in the D group compared with the rest of the groups. No significant differences were noticed across the various groups for the total number of white blood cells and lymphocytes rates. In the second time interval (productive stage), significant differences (P<0.01) were noticed across the various groups in the percentages of the total protein (the highest value was 4.75 g/dl; B group) and serum albumin (the highest value was 3.26 g/dl; D group). It is concluded from the current study that the addition of probiotics to the mixtures of laying hens had a significant improvement in the immune parameters in the blood.

Effect of housing environment and hen strain on egg production and egg quality as well as cloacal and eggshell microbiology in laying hens

This study was conducted to determine the effect of housing environment and laying hen strain on performance, egg quality, and microbiology of the cloaca and eggshell. A total of 1,152 Hy-Line Brown (HB) and Hy-Line W-36 White Leghorn (W-36) hens were used. All hens were kept in conventional cages (CC) from 18 to 32 wk of age and then moved to either enriched colony cages (EC) or free-range (FR) pens or continued in CC. Hens were randomly allocated into a 2 × 3 factorial arrangement of 2 laying hen strains (brown and white) and 3 housing environments (CC, EC, and FR) in a split plot in time (hen age) design. The experiment was conducted from 32 to 85 wk of age. The experiment was divided into 2 phases: early phase (32–51 wk of age) and late phase (52–85 wk of age). A 3-way interaction was observed for hen day egg production (HDEP) among housing environments, hen strain, and bird age in the early phase (P = 0.004) as well as in the late phase (P < 0.0001). In both of the phases, HDEP was higher in CC and FR than in EC. Hy-Line W-36 hens raised in EC had the lowest HDEP compared to other treatments. A 3-way interaction was observed for feed intake (FI; P = 0.017) and feed conversion ratio (FCR) in the late phase (P < 0.0001). The lowest FI and highest FCR were observed in EC for W-36 hens. Free-range hens performed in-between for eggshell quality when compared to CC and EC while HB had better egg quality than W-36. Free-range hens had higher cloacal bacterial counts for aerobes, anaerobes, and coliforms than CC and EC. Higher eggshell bacterial contamination was observed in eggs from FR versus eggs from CC and EC. These results indicate that both housing environment and laying hen strain affect performance and egg quality as well as cloacal and eggshell microbiology. Further studies should be conducted to determine food safety and economic impacts when using different hen strains and housing environments.

The effect of betaine supplementation on performance of laying hens in the tropical climate during the starting period

The study investigated the effects of dietary betaine on the growth performance of laying hens in a tropical climate during the starting period. In total, 192 laying hens strain Lohmann aged three weeks were assigned to four dietary treatments, each consisting of six replicates of eight birds. The dietary treatments included a basal diet (T0) and a basal diet supplemented with betaine at 6 mg/kg (T1), 12 mg/kg (T2), 18 mg/kg (T3). The treatments lasted for eight weeks. Temperature and relative humidity were measured with Hobo-U12, while the wet-bulb temperature was measured using Lutron HT-3027SD. The effect of betaine was not consistent where T3 decreased feed intake (FI) in week 2 but increased FI in weeks 4 and 5. Furthermore, T2 showed higher FI in weeks 6 and 7 than T0 (p<0.05). Accordingly, T2 and T3 led to lower weight gain in week 2, but they compensated with higher weight gain in week 4 when ambient temperature above 30°C occurred for 42 hours in a week. Overall, weight gain and body weight in week 8 was not affected by the treatment. It is concluded that the effect of betaine was more apparent when the birds were exposed to high ambient temperature.

Different Non-cage Housing Systems Alter Duodenal and Cecal Microbiota Composition in Shendan Chickens.

Housing systems are among the most important non-genetic factors affecting hen production performance and intestinal microbes. With increased interest in animal welfare, cage-free laying hen housing systems have become common, providing behavioral freedom and health benefits. The present study aimed to compare the effects of plastic net housing system (NRS) and floor litter housing system (LRS) on the composition and function of the duodenal and cecal microbiota in Shendan chicken, one of the most popular laying hen strains in China. The associations between the differential microbiota abundance and production traits and intestinal morphological parameters were determined. Compared with the LRS, the NRS improved the laying rate (p < 0.05) and increased the villus height (VH) of the duodenum (p < 0.05) and the VH-to-crypt depth ratio (VCR) of the cecum (p < 0.05). Alpha diversity analysis showed that LRS chickens had a significantly higher diversity and richness than NRS chickens. Beta diversity analysis demonstrated differences in the microbiota composition based on housing systems. Within the cecum, Proteobacteria and Kiritimatiellaeota were significantly more abundant in the LRS than in the NRS (p < 0.05), while Bacteroidetes were significantly less abundant in the LRS (p < 0.05). Phascolarctobacterium and Ruminococcaceae_UCG-005 were significantly less abundant in the LRS (p < 0.05) compare to the NRS. Within the duodenum, Lactobacillus was significantly less abundant in the LRS (p < 0.05) than in the NRS, while Pseudomonas was significantly more abundant in the LRS (p < 0.05). Cecal Phascolarctobacterium and Ruminococcaceae_UCG-005 were significantly positively correlated with eggshell strength (R = 0.608, p < 0.01) and egg weight (R = 0.526, p < 0.05), respectively. Duodenal Lactobacillus was significantly positively correlated with VH and VCR (R = 0.548 and 0.565, p < 0.05), while Pseudomonas was significantly negatively correlated with the Haugh unit (R = −0.550, p < 0.05). In conclusion, there are differences in the cecal and duodenal microbiota compositions of Shendan laying hens reared in different non-cage housing systems, and the NRS was superior to the LRS in improving the laying performance and intestinal morphology and microecological environment.

Temporal Trends in Performance and Hatchability Traits of Eight Strains of Hens Covered by the Gene Pool Protection Programme in Poland

Abstract The aim of the study was to determine trends in performance and hatchability traits of eight strains of hens that have been covered by the gene pool protection programme in Poland over ten years/generations. The study involved conservation populations of laying hens: Greenleg Partridge (Z-11), Yellowleg Partridge (Ż-33), Sussex (S-66), Leghorn (H-22, G99), Rhode Island Red (R-11, K-22) and Rhode Island White (A-33), which were maintained at the Experimental Station of the National Research Institute of Animal Production in Chorzelów. The following productive traits were analysed for each population: body weight at 20 wk (g), egg weight at 33 and 53 wk, sexual maturity and number of eggs laid per hen up to 56 wk of age. Mortality and culling were also recorded during rearing and production periods. Furthermore, effective population size (Ne) and inbreeding coefficient in the population (Fx) were calculated for each strain. The analysis of the performance results of the eight strains of hens and their trends indicate that the methods of conservation breeding adopted for these populations enable effective implementation of the conservation programme. The strains were found to differ in all the performance traits subjected to evaluation. Over the 10 generations, the strains examined showed high survival and hatchability parameters during both rearing and production periods. The currently used random mating system proved effective to prevent the populations from an increase of inbreeding. However, a worrying downward trend in body weight was observed in some strains.

Potensi Dedak Padi yang Difermentasi dengan Enzim Fitase dalam Pakan Basal Terhadap Kolesterol Total Darah Ayam Petelur

This study aimed to evaluate the potential of rice bran fermented by the enzyme phytase in basal feed to the total blood cholesterol level of laying hen. This research use an experimental method in vivo with a completely randomized design consisting of six treatments and four replications in each treatment. The experimental animals used 24 48-week-old female laying hen strain Isa Brown. The treatment wa given basal feed substituted with different percentage of fermented rice bran by phytase enzyme and 2% lemuru fish oil supplementation. In this research the amount of fermented rice bran by phytase enzyme substituted on basal feed was: treatment P0 (-) (0%), P0 (+) (0%), P1 (5%), P2 (10%) P3 (15%) and P4 (20%). The results of the statistical analysis showed that there are no significant differences (p&gt; 0.05) on total blood cholesterol levels in all treatments. The conclusion of the research is that rice bran fermented by phytase enzymes tends to decrease total blood cholesterol levels in laying hen.

Immune parameters in two different laying hen strains during five production periods

During life, the number and function of immune cells change with potential consequences for immunocompetence of an organism. In laying hens, studies have primarily focused on early development of immune competence and only few have investigated systemic and lymphatic distribution of leukocyte subsets during adolescence and the egg-laying period. The present study determined the number of various leukocyte types in blood, spleen, and cecal tonsils of 10 Lohmann Brown-Classic and 10 Lohmann LSL-Classic hens per wk of life 9/10, 15/16, 23/24, 29/30, and 59/60, encompassing important production as well as developmental stages, by flow cytometry. Although immune traits differed between the 2 hen strains, identical patterns of age-related immunological changes were found. The numbers of all investigated lymphocyte types in the spleen as well as the numbers of blood γδ T cells increased from wk 9/10 to 15/16. This suggests an ongoing release of lymphocytes from primary lymphoid tissues and an influx of blood lymphocytes into the spleen due to novel pathogen encounters during adolescence. A strong decrease in the number of CTL and γδ T cells and an increase in innate immune cells within blood and spleen were found between wk of life 15/16 and 23/24, covering the transition phase to egg-laying activity. Numbers of peripheral and splenic lymphocytes remained low during the egg-laying period or even further decreased, for example blood CD4+ T cells and splenic γδ T cells. Functional assessments showed that in vitro IFN-γ production of mitogen-stimulated splenocytes was lower in wk 60. Taken together, egg-laying activity seems to alter the immune system toward a more pronounced humoral and innate immune response, with probable consequences for the immunocompetence and thus for productivity, health and welfare of the hens.

Effect of Laying Hen Genotype, Age and Some Interior Egg Quality Traits on Lysozyme Content

Abstract The objective of the study was to evaluate the quality of eggs laid by native breed hens of different ages (33 and 53 weeks of age), with particular consideration of the lysozyme parameters. Eggs were collected from 6 breeds/strains of laying hens under the genetic resources conservation in Poland, i.e. Greenleg Partridge (Z-11), Yellowleg Partridge (Ż-33), Rhode Island Red (R-11), Rhode Island White (A-33), Sussex (S-66) and Leghorn (H-22). The yolks of eggs from older hens had a more favourable, intense colour, but the freshness parameters (albumen height and Haugh units) of these eggs were lower. The albumen of eggs from older hens showed greater content and higher enzymatic activity of lysozyme in both thin and thick albumen. Among the six strains of hens, lysozyme content and enzymatic activity were highest in the egg albumen of strains Ż-33, Z-11 and R-11, and lowest in that of strain H-22. The analysed eggs showed statistically significant correlations between some interior egg quality traits (albumen weight, albumen height, Haugh units, yolk colour) and the discussed enzyme parameters. These traits are influenced by genotype and age of the hens.

Short-Term Spirulina (Spirulina platensis) Supplementation and Laying Hen Strain Effects on Eggs' Lipid Profile and Stability.

Simple SummarySpirulina is an alga rich in micronutrients of interest for improving the nutritional value and quality of the products. This study evaluates the transfer of some of these micronutrients (vitamins and fatty acids) in egg yolk when short periods of supplementation are used in hens of two different strains, and its consequent effects on some quality parameters. Short-term spirulina supplementation markedly modified vitamin content and color in yolk. Hen strain mainly affected the fatty acid profile, and a different response to retinol content and fatty acid proportions (mainly monounsaturated and desaturase activity) according to spirulina supplementation and breed of hens was observed.The aim of the study was to investigate the effect of short-term dietary spirulina supplementation (1% and 3%) and the strain of laying hens (White Leghorn: WL and Rhode Island Red: RIR) on color, nutritional value, and stability of yolk. Egg weight was not affected by any of the studied effects. Yolks from 3%-spirulina supplemented hens had higher retinol and lower α-tocopherol content (p = 0.0001) when compared to control. The supplementation with 1%-spirulina markedly decreased luminosity and increased redness (p = 0.0001) and yellowness (p = 0.0103). Short-term spirulina supplementation slightly modified the fatty acid composition of yolk. The C16-desaturase index increased with the algae, whereas other egg quality indices (hypocholesterolemic, thrombombogenic, n-6/n-3) were not modified. Hen strain mainly affected to the lipid profile. The RIR hens accumulated greater yolk retinol with supplementation doses of 3% (p < 0.05), while the WL hardly suffered changes in the accumulation. Also, yolks from RIR hens had lower C16:0 (p = 0.0001), C18:0 (p = 0.0001), saturated (SAT) (p = 0.0001), and thrombogenic index (p = 0.0001), C20:3n-6 (p = 0.0001), n-6/n-3 ratio (p = 0.003), Δ-6+5-desaturase (p = 0.0005), total elongase indices (p = 0.0001) when compared to WL. Moreover, RIR had higher monounsaturated (MUFA), Δ-9-desaturase and hypocholesterolemic indices (p < 0.05) than WL. A different response to spirulina supplementation was observed for C18:1n-9, MUFA, Δ-9-desaturase and thiesterase indices (p < 0.05) according to hen strain. Yolks from RIR had higher MUFA and Δ-9-desaturase indices than WL at 1%-spirulina supplementation, whereas these parameters were less affected in RIR supplemented with 3%. SAT and Δ-9-desaturase were significantly correlated (r = −0.38 and 0.47, respectively) with retinol content according to a linear adjustment (p < 0.05). Lipid oxidation of yolk was slightly modified by the dietary treatment or hen strain. It was detected a relationship between TBARS and α-tocopherol, C22:5n-3 or C22:6n-3 (p < 0.05). L* and a* were also inversely or positively related with yolk retinol content according to a linear response (p < 0.05). The administration of 1% of spirulina in diets of red hens would be an interesting alternative to get healthier eggs from the nutritional point of view, obtaining an adequate color and without modifications in other yolk quality traits.

Transcriptional responses in jejunum of two layer chicken strains following variations in dietary calcium and phosphorus levels

BackgroundCalcium (Ca) and phosphorus (P) are essential nutrients that are linked to a large array of biological processes. Disturbances in Ca and P homeostasis in chickens are associated with a decline in growth and egg laying performance and environmental burden due to excessive P excretion rates. Improved utilization of minerals in particular of P sources contributes to healthy growth while preserving the finite resource of mineral P and mitigating environmental pollution. In the current study, high performance Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) hens at peak laying performance were examined to approximate the consequences of variable dietary Ca and P supply. The experimental design comprised four dietary groups with standard or reduced levels of either Ca or P or both (n = 10 birds per treatment group and strain) in order to stimulate intrinsic mechanisms to maintain homeostasis. Jejunal transcriptome profiles and the systemic endocrine regulation of mineral homeostasis were assessed (n = 80).ResultsEndogenous mechanisms to maintain mineral homeostasis in response to variations in the supply of Ca and P were effective in both laying hen strains. However, the LSL and LB appeared to adopt different molecular pathways, as shown by circulating vitamin D levels and strain-specific transcriptome patterns. Responses in LSL indicated altered proliferation rates of intestinal cells as well as adaptive responses at the level of paracellular transport and immunocompetence. Endogenous mechanisms in LB appeared to involve a restructuring of the epithelium, which may allow adaptation of absorption capacity via improved micro-anatomical characteristics.ConclusionsThe results suggest that LSL and LB hens may exhibit different Ca, P, and vitamin D requirements, which have so far been neglected in the supply recommendations. There is a demand for trial data showing the mechanisms of endogenous factors of Ca and P homeostasis, such as vitamin D, at local and systemic levels in laying hens.

Effect of the Pellet and Mash Feed Forms on the Productive Performance, Egg Quality, Nutrient Metabolism, and Intestinal Morphology of Two Laying Hen Breeds

Simple SummaryAlthough there is an established understanding of the nutritional requirements for poultry production, the evaluation of the feed form for chickens still needs to be further examined. It is generally believed that a pellet diet has a positive effect on chicken growth, but there are some studies that have reported no difference between pellet and mash on chicken’s performance. The present study indicated that pellet diets improved the production performance and nutrition metabolism through positive changes in the laying rate, feed intake, egg albumen quality and apparent digestibility of laying hens.One of the most important factors that determine feed utilization by chickens is the feed form. Although it is generally believed that pellet diets have a positive effect on chicken growth, there are some studies that have indicated no difference between pellet and mash on chickens performance. This study was conducted to assess the effects of feed form on production performance, egg quality, nutrient metabolism and intestinal morphology in two breed laying hens. Two hundred and sixteen 25-week-old Hy-Line brown (n = 108) and Hy-Line grey (n = 108) hens were selected. Each breed was randomly allocated into two treatments with 6 replications (9 birds in each replication), which were fed mash and pellet diets, respectively. Production performances were recorded daily and egg quality traits were measured every two weeks. At 42 weeks of age, one bird per replication from each experimental group was selected for metabolism determination and intestine morphology observation. Compared with mash diets, pellet diets improved laying rate (p < 0.05), ADFI (average daily feed intake, p < 0.05), egg weight, shell strength, yolk proportion and Haugh unit (p < 0.05) in both breeds and reduced the FCR (feed conversion ratio, p < 0.05) in Hy-Line grey. The apparent digestibility of DM% (dry matter) and CP% (crude protein) were significantly higher (p < 0.05) in both breed laying hens fed pellet than those fed mash. The apparent digestibility of P% (phosphorus) and Ca% (calcium) was higher in Hy-Line grey fed pellet and was higher in Hy-Line brown fed mash. Compared to mash diets, pellet diets increased the VH (villus height), CD (crypt depth) and VCR (ratio of villus height to crypt depth) of the small intestine of Hy-Line grey, and increased the VH and CD of duodenum and ileum of Hy-Line brown. Overall, pellet diets improved production performance and nutrition metabolism through positive changes in the laying rate, feed intake, egg albumen quality and apparent digestibility of laying hens. The current findings provided support for the advantages of feeding pellets during the peak egg laying period for the two popular laying hen strains, Hy-Line brown and Hy-Line grey.

Effects of the housing environment and laying hen strain on tibia and femur bone properties of different laying phases of Hy-Line hens

This study aimed to determine the effect of the housing environment and laying hen strain on tibia and femur properties. A 3 × 2 factorial arrangement of 3 housing environments (conventional cages [CC], enriched colony cages [EC], and free range [FR]) and 2 laying hen strains (Hy-Line W-36 [W-36] and Hy-Line Brown [HB]) in a completely randomized design was conducted from 32 to 85 wk of age. Six left tibias were collected at 8 different time points (38, 45, 52, 59, 65, 72, 79, and 85 wk of age), whereas 6 left femurs were collected at 3 time points (38, 65, and 85 wk of age). Tibias were evaluated for tibia breaking strength (TBS) and ash percentage, whereas femurs were evaluated for bone mineral density (BMD), bone mineral content, bone volume as a fraction tissue volume, and porosity percentage from total, cortical, medullary, and trabecular bones. The higher TBS (P = 0.0005) and ash percentage (P = 0.045) was observed in hens raised in FR systems compared with those raised in the CC. Overall, TBS of W-36 hens was significantly greater than that of HB hens (P < 0.0001); however, there was no difference in the ash percentage between the strains (P > 0.05). An interaction between the housing environment and hen strain was observed for BMD (P = 0.04), wherein W-36 hens raised in the FR system had higher BMD than HB hens. Similarly, hens raised in FR systems had higher trabecular bone volume than those raised in CC (P = 0.022). Hen strain influenced total and cortical bone properties: BMD, bone volume as a fraction tissue volume, and porosity percentage, wherein W-36 hens had better properties than HB hens (P < 0.05). Trabecular BMD was higher in W-36 hens than in HB hens (P = 0.04), whereas bone volume was higher in HB hens (P < 0.0001). The results suggest that raising laying hens in alternative housing systems that have provision for exercise such as FR reduces structural bone loss, stimulate structural bone formation, and improve breaking strength of bones; however, it varies with the strain.

Immunomodulatory Effects of Dietary Phosphorus and Calcium in Two Strains of Laying Hens.

Simple SummaryPhosphorus and calcium are essential nutrients for body functions including the immune system and are generally supplemented to poultry diets. Phosphorus is also present in plant feedstuffs, bound as phytate, which can be used by enzymatic hydrolyzation in the chicken. A reduction of dietary mineral phosphorus might consequently be conceivable, without negatively influencing the immune system. The high concentration of calcium in diets for laying hens that is needed for eggshell formation may inhibit phytate degrading enzymes, and thus, decrease phosphorus availability for the hen. Both phytate degradation and several immune parameters are known to be strain-specific, making an interaction of the genetic background and the dietary phosphorus and calcium supply with the immune system likely. The aim of the study was to evaluate the impact of reduced concentrations of dietary phosphorus and calcium on the peripheral and gut-associated immune system in two laying hen strains. Reduced mineral phosphorus enhanced, while reduced calcium reduced several immune parameters. The two strains showed differences in many immune parameters, but only the impact of dietary phosphorus was influenced by the genetic background. These results suggest that dietary phosphorus and calcium supply may strain-specifically influence immune defense and protection against infection in chicken.Insufficient nutrient supply can impair the immune system, which is important for animal health and welfare. Since chicken can partly hydrolyze phytate, which is the primary phosphorus storage in plant seeds, a reduction of mineral phosphorus in the diets could be an option for more sustainable egg production. Laying hens require high concentrations of calcium that might inhibit the function of endogenous enzymes for phytate hydrolyzation. The objective of this study was to characterize the impact of standard and reduced dietary phosphorus and calcium concentrations on the number and functionality of immune cells in the peripheral and gut-associated immune system in a white and brown laying hen strain. Reduced mineral phosphorus enhanced several immune parameters such as B cells in blood and IgA concentrations in bile in both strains, and peripheral monocytes and γδ T cells in cecal tonsils in brown hens. Reduced calcium levels resulted in lower numbers of T cells in blood and cecal tonsils in both strains, suggesting negative effects on adaptive immunity. Differences between the two strains were found in almost all immune parameters. Results suggest a potentially beneficial effect of reduced dietary mineral phosphorus on the immune system that is dependent on the genetic background.

Thermal Response of Three Strains of Hens Housed in a Cage-Free Aviary at the Amazon Rainforest

ABSTRACT This study aimed to evaluate the thermal response of three strains of hens housed in a cage-free system at the Amazon rainforest in order to evaluate how feather coverage influences thermal exchange with the environment. The experimental method was completely randomized and treatments comprised three strains of hens (Rhode Island Red (red feathers with feathers on the neck), alternative strain FCI (red feathers without feathers on the neck), and alternative strain FCIII (white feathers without feathers on the neck)), with 20 hens (replicates) analyzed per strain. Thermal images of each bird were captured in order to record the birds’ surface temperatures on five points in five targets. All data collected in this study were subjected to ANOVA and subsequently to the Tukey test at p≤0.01 and p≤0.05. The aviary’s left wall presented a lower average temperature, indicating lower heat accumulation, while the floor presented higher heat accumulation. FCIII hens (white feathers) presented higher (p<0.05) heat accumulation on the head and legs, and lower (p<0.05) heat accumulation on the neck and back in relation to other analyzed hens, indicating increased heat exchange efficiency and high concentration of this process in specific body areas. FCI and FCIII hens (without feathers on the neck) presented lower (p<0.05) heat accumulation on the neck and higher (p<0.05) heat accumulation on the head and legs, indicating that the feather coverage directly influenced heat exchange mechanisms, and an increased area without feathers provided great heat exchange zones for birds in a tropical climate.

The impact of management, husbandry and stockperson decisions on the welfare of laying hens in Australia

The present review examines the impact of management and husbandry decisions on the welfare of laying hens in Australia. The literature on many of these aspects is lacking for the Australian egg industry, and, indeed, for the egg industry in general. Management decisions that can affect hen welfare relate to the initial farm design, husbandry routines, and staff selection and training. As modern laying houses represent a considerable financial investment, the decisions made during the design phase are likely to affect both the hens and stockpeople for substantial periods. Hens in cage systems may benefit from fewer tiers and greater space allowances. In non-cage systems, the brown genotypes used in the Australian egg industry may benefit from lower structures that accommodate their heavier and less agile bodies. Keel fractures can be reduced by improving the skeletal health and spatial cognition of laying hens during the rearing period, in addition to minimising the distances they need to jump when navigating aviary structures. The addition of a wintergarden to fixed free-range systems appears to be beneficial. Housing hens in mobile units on free-range farms may challenge their welfare, particularly in relation to heat stress. There is also room for improvement in biosecurity practices and health monitoring of hens, as these appear to be lacking at some farms. The current strains of hen used in free-range systems may not be best suited to these conditions, on the basis of their body condition and flock uniformity. Feed quality may also need to be monitored for quality assurance and optimal hen nutrition. Hen welfare during depopulation can be improved through staff training and by reducing staff fatigue. Euthanising spent hens on farm offers welfare benefits over transporting spent hens to an abattoir. Both hen welfare and working conditions for stock people should be considered when designing laying houses to provide suitable conditions for both hens and stockpeople. This will help improve the job satisfaction of stockpeople, which may translate into better care for the hens and may aid in retaining quality staff. Stockpeople must be recognised as vital contributors to hen welfare in the egg industry, and it is important for the egg industry to continue to attract, train and retain skilled stockpeople to ensure that they enjoy their job and are motivated to apply best-practice care for their flocks. Promoting the animal-care aspect of stockmanship in combination with a supportive managerial environment with optimal working conditions may increase the attractiveness of the egg industry as a place to work.

Edible Environmental Enrichments in Littered Housing Systems: Do Their Effects on Integument Condition Differ Between Commercial Laying Hen Strains?

Simple SummaryFeather pecking can occur in laying hens. It is considered a behavioral disorder and can be triggered by a variety of factors. Feather pecking leads to plumage damage, which is detrimental to the welfare and performance of the animals. The behavior of allopecking is assigned to foraging and exploration behavior. Therefore, a possible approach to reduce feather pecking is to provide manipulatable, edible objects to direct the hens’ pecking to these substrates. Previous studies could not clearly answer the question of the effects of providing such environmental enrichment materials on plumage condition and performance. In order to clarify this question and investigate possible differences between different breeding lines, 4000 pullets were kept during rearing and 2808 hens were kept during the laying period with or without additional enrichment materials (pecking stones and alfalfa bales). The results showed that the effect of enrichment materials on plumage condition and also on pecking injuries of the skin differed between genetic strains. Therefore, the recommendations for the use of enrichment materials should be revised and specified for the hybrid lines.The aim of this study was to investigate the effect of additional enrichment materials (EMs; pecking stones and alfalfa bales) on the occurrence of plumage damage, skin injuries, and toe injuries, with an emphasis on the possible differences between commercial hybrid strains of laying hens. During rearing (weeks 1–18, 16 compartments, 4000 pullets) and laying periods (weeks 21–72, 24 compartments, 2808 hens) in a littered housing system, EMs were permanently provided to the study groups (EXP), while control groups (CON) did not receive additional EM. In a two-factorial study design (two groups with four strains) with 351 hens per variant, the brown egg-laying Lohmann Brown classic (LB) and Bovans Brown (BB) strains as well as the white egg-laying Lohmann Selected Leghorn classic (LSL) and Dekalb White (DW) strains were investigated. Compared to the CON, the EXP showed reduced body mass during rearing (p < 0.001) and reduced albumen consistency in the laying period (p < 0.001). Regarding integument condition, the LSL in the EXP showed more toe injuries than in the CON (p = 0.018). Remarkably, genotype-environment interactions between strains and groups were evident (p < 0.001). In groups with an EM supply, plumage damage decreased in LB (p ≤ 0.033) and LSL (p ≤ 0.005) but increased in BB (p ≤ 0.003). Moreover, there were fewer skin injuries in LSL (p = 0.001) but more in BB (p = 0.001) in groups with access to EM. In view of the diverging effects between strains, future practical recommendations for laying hen husbandry should be strain-specific.

Phytate Degradation, Transcellular Mineral Transporters, and Mineral Utilization by Two Strains of Laying Hens as Affected by Dietary Phosphorus and Calcium.

Simple SummaryPhosphorus is stored as phytate in plant seeds, which are the main components of poultry feed. Poultry can utilize phytate phosphorus after its cleavage catalyzed by enzymes. These enzymes are inhibited by high concentrations of calcium and phosphorus in diets. As laying hens require a high calcium concentration for eggshell production, the inhibition of enzymes might be high. Therefore, the objective of this study was to characterize the degradation of phytate and the utilization of phosphorus and calcium by two laying hen strains supplied with standard or reduced levels of dietary calcium and phosphorus at the egg production peak. The calcium level had a clear effect on phytate degradation products and mineral utilization. The phosphorus level had no effect on these traits, suggesting that actual recommendations for phosphorus supply of laying hens are too high. Differences were noted between the two hen strains and among individual hens regarding calcium and phosphorus metabolism. This is a first step in identifying individual birds that are more capable than others in using phytate phosphorus under challenging conditions. In the long term, our results could help to further reduce the mineral content of laying hen diets.Laying hens require less phosphorus (P) but markedly more calcium (Ca) in their diet than broilers. These differences may cause more distinct interactions with phytate degradation and utilization of minerals in laying hens than those in broilers. The objective of the study was to characterize intestinal phytate degradation, ileal transcript copy numbers of transcellular Ca and P transporters, and mineral utilization by two laying hen strains fed with standard or reduced levels of dietary Ca and P at the laying peak. The strains showed differences regarding several traits driving Ca and P metabolism along the digestive tract. Thus, the two strains may use different mechanisms to meet their respective P demand, i.e., via effective phytate degradation and transcellular transport. Clear effects of the Ca level on myo-inositol concentrations and mineral utilization revealed the significance of this element for the measured traits. The absence of P-mediated effects confirmed the findings of several studies recommending that P concentrations used in laying hen feeds are too high. Differences were noted between individuals within one treatment. The next step would be to evaluate the data in individual birds to identify birds that better cope with a challenging diet.

Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods

The objective of this study was to compare 2 laying hen strains in 5 production periods regarding phytase activity, phytate (InsP6) degradation, and myo-inositol (MI) release in the digestive tract and phosphorus (P) and calcium (Ca) utilization. One offspring of 10 nonrelated roosters per strain (Lohmann Brown-classic (LB) or Lohmann LSL-classic (LSL)) was placed in one of 20 metabolic units in a completely randomized block design in week 8, 14, 22, 28, and 58 of life. All hens were fed the same corn and soybean meal–based diet at one time, but the diet composition was adjusted to the requirements in the respective period. For 4 consecutive days, excreta were collected quantitatively at 24-hour intervals. In week 10, 16, 24, 30, and 60, the blood plasma, digesta of crop, gizzard, jejunum, ileum, and ceca, and mucosa of the jejunum was collected. The concentration of inorganic P in the blood plasma was higher in LB than in LSL hens (P = 0.026). Plasma Ca concentrations increased with each period (P < 0.001) in both strains. In jejunum digesta, the MI concentration did not differ between strains, but InsP6 concentration was higher in LB than in LSL hens (P = 0.002) and the highest in week 30 and 60. Total phosphatase and phytase activities were higher in LB than in LSL hens (P ≤ 0.009). Period effects were also significant for these enzymes. Concentrations of some constituents of the cecal content were different between the strains. The MI concentration in the egg albumen and yolk was higher in LB than in LSL hens. Differences in InsP6- and MI-related metabolism of the 2 hen strains existed. These differences were partly dependent of the period. Especially, week 24 was a period of remarkable change of metabolism. Great differences also existed among individuals, making it worth to have a closer look at the metabolism of individuals in addition to evaluating treatment means. Further studies on metabolic, genetic, and microbiome level may help explain these differences.

Skeletal health of layers across all housing systems and future research directions for Australia

Modern laying hens have been selected for an astounding rate of egg production, but the physiological calcium demand takes a significant toll on their skeletal health. Bones can be assessed both in vivo and ex vivo, using a combination of different structural and mechanical analysis methods. Typically, the properties of leg, wing and keel bones are measured. Conventional caged layers are restricted in movement, which imbalances structural bone resorption and new bone formation, resulting in osteoporosis. Hens within alternative housing systems have opportunities to exercise for strengthening bones, but they can also suffer from higher rates of keel fractures and/or deviations that are likely to have resulted from collisions or pressure force. Limited research has been conducted within Australian commercial housing systems to assess hen skeletal health, including prevalence of keel damage across different system types. Research conducted on both brown and white hen strains approximately within the past decade internationally (2009 onward) has shown that skeletal health is impaired across all housing systems. Keel-bone damage is of specific concern as it occurs at high rates, particularly in multi-tiered systems, is painful, can alter hen behaviour, and reduce both production and egg quality. Management strategies such as the provision of ramps to access perches and tiers can reduce the incidence of keel-bone damage to a degree. Bone strength can be improved through exercise opportunities, particularly when available during pullet rearing. Genetic selection for high bone strength may be necessary for hens to adequately adapt to loose-housed systems, but the best strategy for improving skeletal health is likely to be multifactorial.

A risk assessment of health, production, and resource occupancy for 4 laying hen strains across the lay cycle in a commercial-style aviary system

Different strains of commercial laying hens have been molded by varying selection pressures, impacting their production, health, and behavior. Therefore, assumptions that all laying hen strains use the given resources within aviary systems similarly and maintain equal health and performance may be false. We investigated interactions among patterns of aviary resource use by 2 strains of white and 2 strains of brown laying hens (4 units per strain, 144 hens per unit) with daily egg production, location of egg laying, keel fractures, and footpad damage across the lay cycle. Hens' distribution among resources (litter, nest, wire floor, ledge, and perch) was recorded during light and dark periods at 28, 54, and 72 wk of age. Daily egg production and location were recorded, and 20% of hens per unit were randomly selected and assessed for keel bone damage, foot health, and plumage quality. Production and health risks associated with hens' resource use were assessed using multivariable regression. During the day, more brown hens occupied wire floors, while larger numbers of white hens were on perches and litter. More brown hens were on lower-tier wire floors in the dark, while more white hens occupied top tiers. Brown hens laid more eggs outside nests, showed lower incidence of keel fractures, and had better plumage quality than white hens. White hens had higher odds of keel fractures (4.2) than brown hens. Odds of keel fractures were 3.7 and 5.7 times higher at 54 and 72 wk than at 28 wk in all strains (P ≤ 0.05). Occupying the upper tier at night increased odds of keel fractures by 5.4 times. Occupying perches was associated with lower odds of foot lesions and poor plumage quality in all strains across the lay cycle (P ≤ 0.05). Finally, white hens were associated with lower odds of non-nest laying (0.76), whereas higher nest use by brown hens resulted in higher odds of non-nest egg laying (1.56) across the lay cycle (P ≤ 0.05). Distinct strain differences in resource use in an aviary were associated with different risks to hens' production, health, and welfare.