Stock Solution Research Articles

PM 7/153 (1) Mechanical inoculation of test plants

PM 7/153 (1) Mechanical inoculation of test plants

2× Stock Solution for Nuclear Buffer

2× Stock Solution for Nuclear Buffer

The Effects of Abscisic Acid on Angiogenesis in Both ex vivo and in vivo Assays.

Angiogenesis is a complex biological process wherein novel capillary blood vessels mature from pre-existing vasculature for delivering tissues with oxygen and nutrients. Natural molecules that have anti-angiogenic activity and toxicity can raise the focus on using plant sources as essential therapeutic agent. The current research was intended to estimate the probable anti-angiogenic activity of abscisic acid alone and in combination with prednisolone, a well-known angiostatic glucocorticoid. two months old albino rats were used in this study. ABA and prednisolone stock solutions were prepared and added after embedding aortic rings in growth media. The ex vivo rat aorta ring assay (RAR) was applied to explore the anti-angiogenic effect of ABA. The in vivo chorioallantoic membrane assay (CAM) was applied to quantify the blood vessels inhibition zone by ABA effect. That zone was calculated as the mean inhibition region on eggs in mm ± SD. Abscisic acid screened byex vivo and in vivo assays, revealed a significant dose-dependent blood vessels inhibition in comparison to the negative control with IC50= 7.5µg/ml and a synergism effect when combined with prednisolone. The synergism activity of ABA with prednisolone can significantly inhibit blood vessels growth in RAR and CAM assays. These results shed the light on the potential clinic values of ABA, and prednisolone combination in angiogenesis-dependent tumors.

Integrated and automated high-throughput purification of libraries on microscale.

We herein report the development of an automation platform for rapid purification and quantification of chemical libraries including reformatting of chemical matter to 10mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on analytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.

Antibacterial and Anticandidal Activity of Ethanolic Extract of Immature Flower Buds of Syzygium aromaticum

Microbial resistance to antibiotics has been raised over time, creating a serious burden and an issue that requires a quick response. As a result, interest has shifted to medicinal plants as natural, effective, and cost-efficient alternatives. The goal of this study was to assess the antibacterial and antifungal activities of the dried buds of the clove’s flowers, which were extracted in 250 ml of ethanol using the Soxhlet apparatus. Subsequently, DMSO stock solutions were utilized to create concentrations of 100, 50, 25, 12.5, 6.25, and ultimately 3.125 mg/ml. Following that, the agar-well diffusion technique was utilized for the evaluation of the antibacterial activities of the clove extract against gram-positive bacteria (Staphylococcus aureus and Group A Streptococcus bacteria) and gram-negative bacteria (Escherichia coli), as well as Candida albicans, isolated and identified through the pediatric hospital's medical laboratory west of Gaza city. Lastly, the results of this study clearly showed that the ethanolic extract of dried immature flower buds of clove has anti-S. aureus and anti-group A Streptococcus effects, as well as for the C. albicans used in the present study, with the exception of E. coli, which demonstrated resistance to the plant extract at all concentrations tested. While the lowest concentrations that showed anti-bacterial activity were revealed to be 12.5 and 25 mg/ml for anti-S. aureus and anti-group A Streptococcus, respectively, with inhibition zone diameters of 2 and 1 mm, on the other hand, 25 mg/ml with a 2 mm inhibition zone was determined to have the lowest concentration that had anti-C. albicans activity. This study concluded that cloves can be employed as a plant with anti-bacterial and anti-fungal characteristics, but additional research is required.

DDEL-07. DETERMINATION OF TOTAL AND UNBOUND LEVELS OF A POTENT INHIBITOR OF POLY ADP-RIBOSE POLYMERASE, NIRAPARIB, IN HUMAN PLASMA, CEREBROSPINAL FLUID AND BRAIN TUMOR BY A VALIDATED LC-MS/MS ASSAY

Abstract BACKGROUND Niraparib is an orally available inhibitor of poly ADP-ribose polymerase with high selectivity for PARP1 and PARP2. It is currently approved in the United States for the treatment of ovarian, fallopian tube, and primary peritoneal cancer. Herein we present the development and validation of an LC-MS/MS method for determination of total and unbound levels of niraparib in human plasma, cerebrospinal fluid (CSF) and glioblastoma tissue. METHODS Niraparib was extracted from human plasma, CSF and glioblastoma homogenate samples by protein precipitation with methanol. Unbound fractions of niraparib in plasma and brain tissues were determined by equilibrium dialysis. Validation was performed using Sciex ExionLC UHPLC system coupled with Sciex QTRAP 6500+ MS/MS detector using positive electrospray ionization mode. The method was validated according to FDA guidelines and CAP/CLIA regulations. RESULTS The method was validated for 1-10,000 nmol/L concentration range using plasma as matrix for all biospecimens. The chromatographic separation was performed on Phenomenex Kinetex™ PS C18 column with total run time of 3.5 min with gradient elution. Maximum coefficient of variation for intra- and inter-day precision was 10.6% and the accuracy was within 92.8-118.5% in all matrices. The analyte is stable in plasma and brain homogenate for at least 6 hours at room temperature (RT) and at least 32 days at -20°C. Stability of stock and working solutions was demonstrated for at least 21 hours (RT) and 41 days (4°C). The fraction unbound of niraparib in human plasma and brain were determined to be 0.15 and 0.05, respectively. CONCLUSIONS A sensitive and rapid bioanalytical method is developed and validated to quantify total and unbound niraparib levels in human plasma, CSF and glioblastoma tissue. This method is currently employed to assess plasma pharmacokinetics and CNS penetration of niraparib in newly-diagnosed glioblastoma and recurrent IDH1/2(+) ATRX mutant glioma patients (NCT05076513).

Establishing the lower bacterial concentration threshold for different optical counting techniques

This work compares several physical and optical techniques used in fundamental research and industrial applications to detect bacteria in water. Optical techniques such as, UV-absorbance spectroscopy, laser particle counting, turbidimetry and Z-Sizer light scattering, and a direct observational physical technique, the plate count method, were compared when measuring the concentration of E.coli in tenfold dilution from a stock solution. Estimates of the detection threshold limit of E.coli for the different optical counting techniques and the relationship between colony-forming units (CFU) and tenfold dilutions was established.Optical methods have generated interest due to the rapid response of just minutes, non-destructive approach and minimal sample preparation but their use is still limited to concentrations of up to 4 Log E.coli/mL. In contrast, the plate count method is still a reliable technique for water quality analysis despite its long response time of 24 h.

Anti-proliferative and Apoptosis Inducing Effects of Gallic Acid on Human Acute Myeloid Leukaemia Cell Lines (HL-60)

Gallic acid (GA) comes from benzoic acid or, more specifically, 3,4,5-tryhydroxybenzoic, which derives from the phenolic acid of the non-flavonoid part of the polyphenol compound. It is found ubiquitously in various plants and fruits, such as grapes, gallnuts, pomegranates, and tea leaves [1]. Many scientific journals and articles reported on the pharmacological properties of the photochemical like GA, which has antioxidant properties, antimicrobial, anti-inflammatory, anticancer, cardioprotective, gastroprotective, and neuroprotective activity [2]. Moreover, the anticancer properties of GA have been recognised in several cancers, such as lung cancers, oesophageal cancer cells and leukaemia [3].
 
 The objective of this study is to examine the anti-proliferative and apoptosis inducing effects of GA on HL-60 cell lines. Six concentrations of GA were made using the stock solution of GA compound dissolved in dimethyl sulfide (DMSO). HL-60 cells were treated with concentrations of 100, 50, 25, 12.5, 6.25, and 3.125 µg/mL and was incubated at three incubation period which were 24, 48, and 72 hours. The quantitative measure was determined with cytotoxicity assay of 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and was read by microplate reader at 570 nm. For qualitative measure was through staining with acridine orang (AO) and propidium iodide (PI) and observed the morphological changes of the treated cells.
 
 Results from the MTT assays show that GA has cytotoxicity effect on HL-60 cells especially for 72 hours incubation period. The maximal half inhibitory concentrations (IC50) value of GA decreases as incubation period increases. The IC50 of GA were 9.03, 6.76, and 3.65 µg/ml for 24-, 48-, and 72-hours incubation, respectively. The IC50 value of GA (p<0.05) was significantly different for different incubation periods. The morphological changes were seen through the AO/PI staining with the appearance of the cell blebbing, early apoptosis, and late apoptosis.
 
 Figure 1 shows early apoptosis (EA), late apoptosis (LA), cell blebbing (CB), membrane loose (MB), nuclear fragmentation (NF), and apoptotic bodies (AB).
 
 These findings show that GA has potential as anti-proliferative and apoptosis inducing effects on HL-60 cells line. More research is needed to determine the pathways of apoptosis in HL-60 treated with GA.

Antiproliferative Activity and Apoptotic Potential of Curcumin on Human Acute Myeloid Leukemia Cell Line (HL-60)

Curcuma Longa or also known as turmeric is a prominent natural remedy in Asia that has been utilized to heal diseases for decades. It has been demonstrated to have substantial biological activities, including anticarcinogenic, antimutagenic, antioxidant, anti-inflammatory, anti-infectious, hypocholesterolemic, and chemopreventive properties. Curcumin is a therapeutic substance extracted from Curcuma Longa that is thought to have anti-cancer properties against a variety of cancer types. However, its anti-cancer potential on acute myeloid leukemia (AML) remains uncertain [1,2]. 
 
 The objective of this intervention study was to examine the anti-proliferative and apoptotic effects of curcumin on the HL-60 cell line of acute myeloid leukemia. A stock solution of pure curcumin compound was obtained by dissolving it in dimethyl sulfide (DMSO). HL-60 cells were treated with six different concentrations which were 100, 50, 25, 12.5, 6.25, 3.125 µg/ml and incubated at three distinct incubation period (24, 48 and 72 hours). The in vitro cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and quantified using a microplate reader at 570 nm. Acridine orange (AO) and propidium iodide (PI) staining were employed to evaluate morphological changes. 
 
 MTT assays results show that curcumin has a high cytotoxicity effect on HL-60 cells. The maximal half inhibitory concentration (IC50) value of curcumin was consistently decreased when the incubation time increased. The IC50 of curcumin on HL-60 cells were reported to be 13, 8, and 3.5 µg/ml at 24, 48 and 72 hours of incubation, respectively. There was a significantly different IC50 value (p<0.05) of curcumin between the incubation periods (Figure 1). The morphological alteration was shown in AO/PI staining by the appearance of early apoptosis, late apoptosis, and necrotic cells.
 These findings illustrated that curcumin revealed an effective anti-cancer action on human acute myeloid leukemia HL-60 cell line. Further research is required to determine the mechanism of cell death in HL-60 cells treated with pure curcumin compound.
 
 In conclusion, the study revealed the role of putative targeted genes in the biological mechanism in gout pathogenesis has provides insight of the potential biomarkers in developing the personalized medicine better treatment for gout patient in ensuring better patient healthcare.

Boosting Performance of Inverted Perovskite Solar Cells by Diluting Hole Transport Layer.

In our study, by developing the diluted PEDOT:PSS (D-PEDOT:PSS) to replace PEDOT:PSS stock solution as hole transport layer (HTL) materials for fabricating the inverted perovskite solar cells (PSCs), the performance of developed device with ITO/D-PEDOT:PSS/MAPbI3-xClx/C60/BCP/Ag structure is enhanced distinctly. Experimental results reveal that when the dilution ratio is 10:1, the optimal power conversion efficiency (PCE) of the D-PEDOT:PSS device can reach up to 17.85% with an increase of 11.28% compared to the undiluted PEDOT:PSS device. A series of investigations have confirmed that the efficiency improvement is mainly attributed to the two aspects: on one hand, the transmittance and conductivity of D-PEDOT:PSS HTL are improved, and the density of defect states at the interface is reduced after dilution, promoting the separation and transmission of charges, thus the short-circuit current (JSC) is significantly increased; on the other hand, the work function of D-PEDOT:PSS becomes more consistent with perovskite layer, and the voltage loss is reduced, so that the higher open circuit voltage (VOC) is obtained. Our research has indicated that diluting HTL develops a simpler, more efficient and cost-effective method to further improve performance for inverted PSCs.

A simple spectrophotometric determination of hydrogen peroxide solution via spectral delta from redox reaction with sodium hypochlorite.

Hydrogen peroxide (H2O2) is widely used in the synthesis of organic chemicals, bleaching of paper pulp, and the treatment of wastewater and as a food additive, important mediator of redox processes in natural water, and a disinfectant. However, H2O2 stock solution is unstable and slowly decomposes when exposed to, for example, light, elevated temperatures, or metal compounds. Therefore, the ability to measure the exact concentration of H2O2 stock solution is important for its proper use in diverse applications. This work proposes a simple method for the spectrophotometric determination of H2O2 solution via chemical reaction with sodium hypochlorite that is inexpensive and easy to acquire. The proposed method is based on the stoichiometric spectral change of hypochlorite ion at 292.5nm following a redox reaction with a sample solution of H2O2. Due to high relationship between the spectral delta value and the applied H2O2 concentration (0.00188-0.03000%), H2O2 stock solution can be easily quantified.

Curcumin Supplementation in Semen Extender Improves Post-Thaw Quality and Antioxidant Capacity of Gir Bull Spermatozoa

This investigation was carried out on semen of three Gir bulls at Cattle Breeding Farm, Junagadh (India) for a period of 6 months. The aim was to assess the effect of different concentrations of Curcumin, viz., 0 µM (Control), 5 µM, 10 µM, and 15 µM in Andromed extender on sperm quality along with oxidative stress parameters of cryopreserved semen. Semen ejaculates (n=30, 10/bull) with >70% initial motility were included in the study. Soon after initial evaluation, the semen was extended in Andromed extender @ 80 million sperm per ml and was divided into four equal aliquots. The Curcumin stock solution (10 mM) was added in these aliquots to get a final concentration of 0, 5, 10, and 15 µM and sperm motility was checked. It was soon filled and sealed in medium French straws (0.5 ml), equilibrated at 4°C for 4 h and frozen in LN2 vapour using conventional wide mouth freezer. Thawing of straws was done next day in water bath at 37°C for 30 seconds, and various sperm quality parameters including oxidative markers were assessed. The results showed that supplementation of Curcumin to freezing extender improved semen quality and antioxidant capacity. The post-thaw sperm motility, sperm viability, HOST reactive sperm and acrosomal integrity in semen extended with 15 µM Curcumin concentration (57.33±0.67, 73.20±1.05, 64.77±1.56 and 72.43±1.38 %, respectively) were significantly (p<0.05) higher and sperm abnormality (18.00±0.41%) was significantly (p<0.05) lower as compared to those of control extender and the 5 µM Curcumin group. Mean malondialdehyde (MDA) level in post-thaw seminal plasma did not differ significantly (p>0.05) among the groups, while total antioxidants activity (TAC) with 15 µM Curcumin (476.36±16.52 μmol/L) was significantly (p<0.05) higher as compared to control and 5 µM Curcumin group. It is concluded that Curcumin @ 15 µM in semen extender improves post-thaw sperm quality and antioxidant capacity of cryopreserved Gir bull semen.

Comparison of Wound Healing Effect of Skin Micrograft Impregnated into Two Kinds of Artificial Dermis in a Murine Wound Model.

Skin MG was prepared from the skin of C57BL/6JJcl mice using Rigeneracons and administered onto Pelnac Gplus and Integra sheets. The amount of MG suspension impregnated in Pelnac Gplus and Integra was evaluated. Pelnac Gplus and Integra sheets combined with MG were applied to murine defects, and wound area, neoepithelium length, granulation tissue formation, and newly formed capillaries were compared with the control groups on days 7 and 14. The weight percentage of the MG absorbed by Pelnac Gplus and Integra was 88.8% ± 3.5% and 28.2% ± 7.0%, respectively (P < 0.05). In the in vivo experiment, the area of newly formed granulation tissue and both the number and area of newly formed capillaries in the PelnacG + MG group were significantly larger than those in the control group at 14 days after implantation (P < 0.05). Skin MG was successfully impregnated into Pelnac Gplus by simple administration but not into Integra. Administration of skin MG into the Pelnac Gplus promoted granulation formation and angiogenesis. Pelnac Gplus was more suitable than Integra in the combination therapy.

ODP479 Equilibrium Dialysis ID-LC/MS/MS Procedure for Routine Measurement of Free Thyroxine in Serum

Abstract Free thyroxine (FT4) test is essential for diagnosis of thyroid diseases. Most FT4 tests used in patient care are immunoassays (IAs). However, concerns about the accuracy of FT4 IAs have been raised in the clinical laboratory community. Reference measurement procedure (RMP) for FT4 based on well-defined equilibrium dialysis (ED) of serum has been established and are well recognized. While FT4 RMP is highly accurate and precise, it is not intended for routine clinical use. Our aim is to develop an ED-based FT4 assay that is traceable to the RMP and suitable for routine measurements such as investigations in epidemiologic studies. The described assay is based on ED-LC-MS/MS. FT4 in serum was isolated from protein-bound T4 in serum according to the CLSI C45-A guideline. FT4 levels achieved equilibrium in a commercially available micro-ED plate after 18 hours. The dialysate was collected and spiked with internal standard (13 C 6 -T4). The IRMM-468 certified primary reference material (JRC, Belgium) of T4 was used for assay calibration. FT4 in dialysate was extracted with ISOLUTE-96-well C18 SPE plates (Biotage) before LC-MS/MS analysis. T4 could be resolved from Triiodothyronine (T3), reverse triiodothyronine (rT3), and other interferences in the dialysate on a C18 UPLC column with an elution time of less than 8 min. Selective reaction monitoring in positive electrospray ionization mode was used for detection. The measuring range covered 1-100 pg/mL of FT4. The assay sensitivity allowed detection of 0.5 pg/mL FT4, which is sufficient for detection of FT4 in normal and hypothyroid patient samples. The difference of this method to the CDC FT4 RMP was within ±5%. The imprecision of the routine assay, evaluated over four different days, was less than 10% CV. The slopes of calibration curves in dialysate matrix and water were comparable with each other indicating no significant matrix effect on FT4 quantification. The use of micro-ED plates in combination with an automated liquid handling system allows for effective automation of the procedure. The stability of T4 calibrator stock and working solutions were confirmed at various conditions. Currently, a comprehensive comparison study is underway between the newly developed routine method and the CDC FT4 RMP. The described routine FT4 assay can significantly improve throughput. The sensitivity, precision, and accuracy are suitable for routine FT4 measurements in clinical laboratories and conducting large epidemiologic studies. Applying this FT4 routine method to generate population data will support the use of standardized FT4 measurements and to address data needs on FT4 in general population. Presentation: No date and time listed

Effects of cigarette smoke extract on mitochondrial function of macrophages

Objective: To investigate the effects of cigarette smoke extract (CSE) on the mitochondrial function of macrophages. Methods: RAW264.7 macrophages were used for the experiment in this study. When the cell density was about 70%, the old culture medium was abandoned, and the 100% CSE stock solution was diluted with serum-free DMEM and FBS into 1%, 5%, 15%, 25% and 90% CSE and added to the well plate. The cell activity of RAW264.7 cells treated with CSE at different concentrations for 24 h was detected by CCK-8 method. Then the optimal CSE concentration was selected to treat cells for 0 h, 24 h, 48 h or 72 h respectively, and CCK-8 assay was used to detect the cell activity of CSE treated cells at different time groups. After the cells were treated with 0%, 5% and 25% CSE for 24 hours, cell necrosis and apoptosis was detected by Annexin V-FITC /PI staining; Mitochondrial membrane damage of RAW 264.7 was detected by mitochondrial membrane potential assay kit with JC-1; Macrophages were stained with ROS-specific dye DCFH-DA, and then Flow cytometer was used to determine the fluorescence and the proportion of ROS-positive macrophages; the enhanced ATP assay kit was used to detect the intracellular ATP concentration. Results: ①Compared with 0% CSE, cell viability was increased significantly in 1% CSE group (P＜0.01), cell viability was decreased significantly when CSE concentration was above 5% (P＜0.05); Macrophages were treated with 5% CSE, and cell viability was decreased significantly with the increase of treatment time (P＜0.01). ②Compared with 0% CSE, 5% CSE and 25% CSE mainly caused macrophage necrosis, decreased mitochondrial membrane potential, increased ROS production and decreased ATP significantly (P＜0.05 or P＜0.01), and the changes were more significant in 25% CSE treatment group(P＜0.05 or P＜0.01). Conclusion: CSE may affect mitochondrial function of macrophages, leading to decreased cell viability and necrosis.

Model for prediction of the optimal dose of Jatropha curcas in FS dewatering

Abstract Jatropha curcas (JC) is a highly effective conditioner in dewatering fecal sludge (FS); however, there are limited studies on the model predicting its optimal dose. This study presents the results of the developed model for predicting JC optimal doses. The developed model assessed the FS parameters and JC stock solution. We analyzed the FS samples from a mixture of a pit latrine and septic tank at the water quality laboratory of the University of Dar es Salaam. The multiple linear regression model was used to establish a relationship between JC optimal dose as a function of FS characteristics (pH, electrical conductivity, total solids, total suspended solids and concentration of the JC stock solution). The results indicated that 90.4% of the JC optimum dosage was determined and contributed by FS characteristics and JC stock solution concentrations. Also, the main explanatory factors determining the JC optimal dose were the JC stock solution concentration, followed by the pH of FS. The model results showed a good agreement between the predicted and observed JC optimal dose with a coefficient of determination of R2 = 0.904 and 0.7879 for calibration and validation, respectively. Therefore, the model can be adapted to determine the JC optimal dose without running the jar test experiment.

Fluoride induces morphological change in Fenugreek (Trigonella foenum-graecum L) during germination and early seedling

The goal of this study was to assess the toxicological effects of exposing popular variety of fenugreek [Trigonella foenum-graecum L.] to fluoride during the germination and early seedling stages. In January 2022, the experiment was carried out at Arunachal University of Studies, Namsai. Surface sterilized seeds of uniform size were set for germination in petri-plates over cotton beds, treated with fluoride solutions of varied strengths (0.0, 2.0, 4.0 and 6.0 mM), made from a stock solution of sodium fluoride (50.0mM). The results showed that fluoride might cause considerable phytotoxicity during germination and seedling growth. Fluoride administration resulted 13 to 43.10% suppression of germination from 2.0mM to 6.0mM. Similar results were also found in germination index, germination energy, relative germination rate and seedling vigor index which were significantly reduced by 43.1, 43.1, 34.0, and 84.0% in 150.0 mM, respectively. Fluoride also had significant detrimental impact on seedling length (71.78%) in the intact seven days with increasing doses of fluoride.

Fluoride induces morphological change in Fenugreek (Trigonella foenum-graecum L) during germination and early seedling

The goal of this study was to assess the toxicological effects of exposing popular variety of fenugreek [Trigonella foenum-graecum L.] to fluoride during the germination and early seedling stages. In January 2022, the experiment was carried out at Arunachal University of Studies, Namsai. Surface sterilized seeds of uniform size were set for germination in petri-plates over cotton beds, treated with fluoride solutions of varied strengths (0.0, 2.0, 4.0 and 6.0 mM), made from a stock solution of sodium fluoride (50.0mM). The results showed that fluoride might cause considerable phytotoxicity during germination and seedling growth. Fluoride administration resulted 13 to 43.10% suppression of germination from 2.0mM to 6.0mM. Similar results were also found in germination index, germination energy, relative germination rate and seedling vigor index which were significantly reduced by 43.1, 43.1, 34.0, and 84.0% in 150.0 mM, respectively. Fluoride also had significant detrimental impact on seedling length (71.78%) in the intact seven days with increasing doses of fluoride.

Surrounded by ligands: the reactivity of cisplatin in cell culture medium.

The reactivity of platinum-containing drugs such as cisplatin, carboplatin, and oxaliplatin is essential for its mechanism of action as an anticancer agent. This inherent reactivity means that molecules in tools used to study these metal-based drugs such as solvents (DMSO), cell culture media, and other buffer additives can ligate to and inactivate or activate them. This Commentary considers these cautionary tales in the context of a new report that cisplatin can also react with penicillin, reiterates best practice in creating Pt drug stock solutions, and highlights the significant work that remains to fully characterize the fate of cisplatin in cell culture media.

&lt;i&gt;Acacia nilotica &lt;/i&gt;- A Mild Steel Corrosion Inhibitor in Sulphuric Acid Medium

Plant extracts have become an essential renewable resource and eco-friendly for many applications by using corrosion inhibition. In this study, the inhibitive activity of water concentrates of the Acacia nilotica with 0.5M H2SO4 stock solution at different concentrations, was studied using the Gravimetric Method. To know the improved impact on the consumption of mild steel, extracts of the plant were characterized by Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM). SEM confirmed the formation of a protective layer on the surface of mild steel. Different techniques like UV-Visible and weight-loss analysis were studied to detect the organic natural compounds in the extract. Langmuir isotherm was fitted well for this study with a correlation coefficient (R2) of 0.999.