Stimulate Plant Growth Research Articles

Do sub-doses of glyphosate and 2,4-D cause hormesis in the pineapple plants? Insights into the response of the pineapple plants to this phenomenon

The slow growth of the pineapple (Ananas comosus (L.) Merr.) during the vegetative phase prolongs the crop cycle, resulting in higher production costs. When used in low doses, some herbicides can stimulate plant growth, a phenomenon known as hormesis. The aim of this study was to determine the effects of sub-doses of glyphosate and 2,4-dichlorophenoxyacetic acid (2,4-D) on growth in the Pérola cultivar of the pineapple. The treatments under study consisted of glyphosate applications of 0, 1.8, 3.6, 7.2, 18, 36, 72, 180, 360 and 720 g a.e. ha-1, and 2,4-D applications of 0, 1.68, 3.35, 6.70, 16.75, 33.5, 67, 167.5, 335 and 670 g a.e. ha-1. The experiments were completely randomised with four replications. The plants were grown in the greenhouse in pots with a capacity of 8 dm3 substrate. Growth variables were evaluated in the plants, together with the total and partitioned accumulated biomass (leaf, stem and root). The data were submitted to analysis of variance and regression. The pineapple showed greater phytotoxicity from the glyphosate herbicide at applications ranging from 18 to 720 g a.e. ha-1 and from 2,4-D in the range of 167.5 to 670 g a.e. ha-1. The hormesis effect was evidenced by the 32.25% increase in root fresh weight at the dose of 1.8 g a.e. ha-1 glyphosate. However, the slow growth of the pineapple was not overcome by the hormesis effect of the herbicides.

Interação de plântulas de soja com rizobactérias benéficas

Beneficial rhizobacteria are multifunctional microorganisms that stimulate plant growth through direct mechanisms such as the solubilization of phosphorus and potassium present in soil minerals, biological nitrogen fixation and production of plant hormones. The objective of this study was to determine the effect of beneficial rhizobacteria on the initial development, promoting growth and resistance of soybean seedlings, aiming not only for increased production but also for agricultural sustainability through the maximization of biological efficiency in the early development of soybean seedlings. The experiment, under controlled conditions, was conducted in a completely randomized design, with four treatments and ten replications. The treatments consisted in the microbiolization of soybean seeds with: 1. Control (without microorganism); 2. Serratia marcenses (BRM 32114); 3. Bacillus spp. (BRM63573) and 4. BRM 32114 + (BRM63573). After the microbiolization of the seeds, they were sown and placed to germinate, after 12 days, the seedlings were harvested and divided into shoots and roots. The roots were washed and photographed with digital camera. The images were analyzed and determined length, diameter, surface area and volume of roots. Then, root and shoot were dried and weighed for biomass determination. Co-inculation, BRM 32114+ BRM63573, promoted greater robustness of the root system of soybean seedlings with increased length (16.2%), diameter (22.5%), volume (43.4%) and dry biomass (29.7%). The dry matter of shoots and total soybean seedlings was superior to the control treatment in all treatments with rhizobacteria. Therefore, plant growth promoting

Use of protein-based matrices as amino acids source in in-vitro grapevine

ABSTRACT Proteins are sources of peptides and amino acids which are able to stimulate plant growth. Protein-based matrices are a novel source of these raw materials since they allow their availability in small, prolonged doses, which could be of interest in in-vitro assays. Thus, this work aimed to evaluate the use of soy protein-based matrices in in-vitro cultures of grape, cv. Magliocco Canino. Their influence was assessed in different media conditions in the presence or absence of zinc (an essential microelement for plant growth). The shoots were evaluated after 35 days of growth based on their growth parameters (weight increase, number of stems, number of leaves, stem and internode length). A biochemical profile of the shoots cultivated in different media was obtained by Fourier-Transform Infrared Spectroscopy (FTIR). The results highlighted the benefits of using protein-based matrices in in-vitro culture as shoots showed an increase in weight, number of leaves, and longer stems, also in zinc-deficient media. In conclusion, this work emphasises the potential of protein-based matrices as stimulants for grapevine explants. This could have important implications for the cultivation of these crops and could be the starting point for further studies on the stimulating effect of different proteins on crops.

Exploring the synergistic effects of goethite intercalated coal in the presence of humic acids for enhanced growth of Sinapis alba

BackgroundIron oxide mineral–humic complexes serve as a reservoir of bioavailable Fe for plants, releasing metal ligands and providing Fe–humic complexes directly usable by plant Fe-uptake mechanisms. In this study, we synthesized and characterized goethite α-FeOOH (G) nanoparticles (NPs) intercalated in coal (GC) to estimate the bioactivity effect of humic acids (HA). The synthesized GC NPs were characterized by X-ray diffraction, scanning electron microscopy (SEM), Mössbauer spectroscopy, N2 adsorption–desorption Brunauer–Emmett–Teller (BET) specific surface area, zeta potential, hydrodynamic particle diameter, iron ions release, and a phytoassay method of root elongation using the higher plant Sinapis alba.ResultsX-ray diffraction revealed that G was the primary phase in both GC and GC–HA complexes. Mössbauer spectroscopy analysis identified a goethite-doped Fe2+-in the GC samples. The intercalation of G into the coal matrix increased the specific surface area of GC, enhancing its HA sorption capacity. In addition, GC–HA demonstrated superior plant growth stimulation compared to HA and GC alone, indicating its role in colloidal stability. In contrast to GC, GC–HA exhibited a more consistent and time-dependent release of Fe3+ and Fe2+. This sustained Fe release from GC–HA, coupled with the formation of Fe3+ and more bioavailable (soluble) Fe2+ humic complexes is a promising result in terms of iron nanofertilizers production.ConclusionsThe use of goethite nanoparticles intercalated within a coal matrix and subsequently complexed with HA contributes to prolonged phytoactivity by employing slowly released nutrient additives within the coal mesoporous matrix.Graphical

Nutritional Value of Parsley Roots Depending on Nitrogen and Magnesium Fertilization

Parsley is an herb/vegetable rich in nutritional compounds such as carbohydrates, vitamins, protein, crude fiber, minerals (especially potassium), phosphorus, magnesium, calcium, iron, and essential oils. Limited information is available in the literature on the quality of parsley roots depending on the cultivation technology used in the form of macronutrients and micronutrients, preparations to stimulate plant growth and development, as well as plant-protection products. A three-year study was undertaken to determine the effect of applying mineral fertilization with nitrogen, including magnesium on the nutritional value of parsley roots in terms of the content of ascorbic acid, total and reducing sugars, and minerals: (total N, K, Mg, Ca). The research material was the root of Petroselinum crispum ssp. tuberosum from an experiment where nitrogen was applied in soil at (0, 40, 80, 120 kg N ha−1) and magnesium at (0, 30 kg MgO ha−1). Nitrogen fertilization increased the nutritional value in terms of total and reducing sugars, as well as total N and Ca content. Applied magnesium fertilization caused a significant increase in the content of all tested nutrients. The most total sugars (127.7 g kg−1 f. m.), reducing sugars (16.8 g kg−1 f. m.), and total N (12.13 g kg−1 d. m.) were accumulated by roots from the object where nitrogen was applied at a maximum rate of 120 kg N ha−1, including magnesium. On the other hand, for the content of K (19.09 g kg−1 d. m.) in the roots, a dose of 80 N ha−1 was sufficient. For ascorbic acid (263.2 g kg−1 f. m.) and Ca (0.461 g kg−1 d. m.), a dose of 40 kg N ha−1 with a constant fertilization of 30 kg MgO ha−1 was sufficient. When applying high doses of nitrogen, lower doses of magnesium are recommended. This is sufficient due to the high nutritional value of parsley roots. Due to the worsening magnesium deficiency in soils in recent years, the use of this nutrient in the cultivation of root vegetables is as justified and timely as possible. Quality-assessment studies of root vegetables should be continued with higher amounts of magnesium fertilization. Different ways of applying magnesium in parsley cultivation should also be tested.

Corn yield in the application of doses and forms of nitrogen fertilizers in the Shaulder irrigation massif of Turkestan region

Nowadays, to obtain high and stable yields of agricultural crops, in addition to the creation and introduction of new high-yielding varieties, the use of various plant protection products, growth stimulants and biopreparations, the effective use of mineral fertilizers is a necessary agricultural technique. The purpose of this work was to study the influence of various doses and forms of nitrogen fertilizers on the dynamics of the accumulation of raw mass of corn plants, crop structure, yield, as well as the removal and normative consumption of nutrients on different salinization backgrounds in the Shaulder irrigation massif of Turkestan region. The obtained results showed that the best indicators were established in a slightly saline background than in an average saline one. On average, on a slightly saline background, the yield of corn grain, depending on the forms of nitrogen fertilizers at a dose of 80 kg / ha, varied in the range of 13.2-14.9 t/ha. At the same time, the yield gain from the use of various forms of fertilizers at the same doses of nitrogen amounted to 21.6-37.4 %. Against a medium-saline background, the option with the use of ammonium nitrate (variant 5) with an addition of 47.7 % nitrogen fertilizer to the phosphorus-potassium background. Regression models were obtained that accurately reflect the dependences of the grain yield and the elements of the corn crop structure on the total effect of nitrogen fertilizer (x1) and the degree of soil salinity (x2): Y = 18.429 + 0.2656x10.5 - 37.0995x2 with a high coefficient of determination - R2 = 0.954. On a slightly saline background, nitrogen removal by the corn grain harvest with the corresponding amount of by-products according to the experimental options varied from 282.9 kg/ha in the P80K80 option, to 370.1 kg/ha in the P80K80 + N120 option (ammonium sulfate), phosphorus – from 121.4 kg/ha in the P80K80 + N80 option (urea) to 160.7 kg/ha in the P80K80 + N80 option (ammonium nitrate), potassium – from 325.2 kg/ha in the P80K80 + N80 option (urea) to 413.7 kg/ha in the P80K80 + N80 option (ammonium sulfate). On a medium-saline background, nitrogen removal was in the range of 175.3–223.8 kg/ha, phosphorus - 67.4-89.6 kg/ha, potassium – 178.5-217.3 kg/ha. The utilization rate of nitrogen from fertilizers varied significantly depending on the doses and forms of nitrogen fertilizers and the degree of soil salinity.

Quality and safety of a plant growth stimulant obtained from poultry by-products

Poultry farming is one of the most efficiently developing agricultural industries thanks to the high level of technology and automation of enterprises. However, the intensive pace of development in poultry farming leads to several problems; one of the most important among them is the need to dispose of poultry waste. Large poultry farms can produce up to 100 thousand tons of manure annually, which belongs to the 3rd class of environmental hazards. Recycling manure allows enterprises to avoid penalties and receive additional economic benefits from selling organic fertilisers. Mechanochemical methods are one of the most accessible and effective ways to process livestock waste. Their essence lies in using chemical reagents for disinfection and processing raw materials. The object of the study was a new drug with the properties of a growth stimulant, antidepressant, and antistressant obtained by mechanochemical processing of dry bird manure in an acidic environment. The safety parameters were analysed in two accredited laboratories: agrochemical and veterinary. The agrochemical laboratory studied such indicators as the mass fraction of dry and organic matter, the activity of hydrogen ions (pH), the mass fraction of nutrients, the content of toxic elements and the presence of radionuclides. The veterinary laboratory analyses included such groups of indicators as parasitic cleanliness, sanitary-bacteriological, and sanitary-parasitological indicators. It has been shown that the leading indicators of the quality and safety of the drug in the working solution meet the requirements for organic fertilisers. For the medication to work effectively, when preparing a working solution, the consumer must strictly follow the instructions and observe safety precautions when working with a substance with a low pH level.

Digging into the Lettuce Cold-Specific Root Microbiome in Search of Chilling Stress Tolerance-Conferring Plant-Growth-Promoting Bacteria

Growth of lettuce ( Lactuca sativa) is severely hampered by low temperatures, even when cultivated under greenhouse conditions. Root-associated bacteria might promote plant growth under stressful conditions. Therefore, we analyzed the effect of low temperatures on the lettuce root-associated microbiome to evaluate whether microbiome-based selection aids in identiying bacteria that stimulate plant growth in the cold. 16S ribosomal RNA gene amplicon sequencing was used to examine the compositional differences in the lettuce root-associated microbiome when grown under low- and control-temperature conditions. Chilling temperatures significantly altered the lettuce root endosphere composition, whereas their effects were less severe in the rhizosphere and absent in the bulk soil. Several cold-enriched families were found in both the rhizosphere and the root endosphere, nine of which were Oxalobacteraceae, Pseudomonadaceae, Flavobacteriaceae, Microscillaceae, Sphingobacteriaceae, Comamonadaceae, Devosiaceae, Methylophilaceae and env.OPS_17. Concurrently, a collection of lettuce root-colonizing bacteria was established and, based on correlation with these families, representative isolates were screened. None of the lettuce root isolates showed growth-promoting effects but three growth-promoting Flavobacterium strains from an available collection of grass root-colonizing bacteria were identified. Amplicon sequence variant (ASV) annotation of the lettuce and grass strains revealed that strains matching cold-enriched or highly abundant ASVs in at least one soil promoted growth in the cold. Overall, our data demonstrate that microbiome analyses, combined with high-throughput bacterial isolations might be a helpful tool to isolate effective cold growth-promoting strains.

Radiation Hormesis in Barley Manifests as Changes in Growth Dynamics Coordinated with the Expression of PM19L-like, CML31-like, and AOS2-like.

The stimulation of growth and development of crops using ionising radiation (radiation hormesis) has been reported by many research groups. However, specific genes contributing to the radiation stimulation of plant growth are largely unknown. In this work, we studied the impact of the low-dose γ-irradiation of barley seeds on the growth dynamics and gene expression of eight barley cultivars in a greenhouse experiment. Our findings confirmed that candidate genes of the radiation growth stimulation, previously established in barley seedlings (PM19L-like, CML31-like, and AOS2-like), are significant in radiation hormesis throughout ontogeny. In γ-stimulated cultivars, the expression of these genes was aligned with the growth dynamics, yield parameters, and physiological conditions of plants. We identified contrasting cultivars for future gene editing and found that the γ-stimulated cultivar possessed some specific abiotic stress-responsive elements in the promotors of candidate genes, possibly revealing a new level of radiation hormesis effect execution. These results can be used in creating new productive barley cultivars, ecological toxicology of radionuclides, and eustress biology studies.

N- acyl homoserine lactones (AHLs) type signal molecules produced by rhizobacteria associated with plants that growing in a metal(oids) contaminated soil: A catalyst for plant growth

The present study explores the potential of rhizobacteria isolated from Baccharis linearis and Solidago chilensis in metal(loid)-contaminated soil for producing N-acyl-homoserine lactones (AHLs)-type signal molecules and promoting plant growth. A total of 42 strains were isolated, four demonstrating the production of AHL-type signal molecules. Based on 16S rRNA gene sequencing analyses and MALDI-TOF analyses, these four isolates were identified as belonging to the Pseudomonas genus, specifically P. brassicacearum, P. frederickberguensis, P. koreensis, and P. orientalis. The four AHL-producing strains were evaluated for metal(loid)s tolerance, their plant growth promotion traits, AHL quantification, and their impact on in vitro Lactuca sativa plant growth.The study found that four strains exhibited high tolerance to metal(loid)s, particularly As, Cu, and Zn. Additionally, plant growth-promoting traits were detected in AHL-producing bacteria, such as siderophore production, ammonia production, ACC deaminase activity, and P solubilization. Notably, AHL production varied among strains isolated from B. linearis, where C7-HSL and C9-HSL signal molecules were detected, and S. chilensis, where only C7-HSL signal molecules were observed. In the presence of copper, the production of C7-HSL and C9-HSL significantly decreased in B. linearis isolates, while in S. chilensis isolates, C7-HSL production was inhibited. Further, when these strains were inoculated on lettuce seeds and in vitro plants, a significant increase in germination and plant growth was observed. Mainly, the inoculation of P. brassicacearum and P. frederickberguensis led to extensive root hair development, significantly increasing length and root dry weight.Our results demonstrate that rhizospheric strains produce AHL molecules and stimulate plant growth, primarily through root development. However, the presence of copper reduces the production of these molecules, potentially affecting the root development of non-metalloid tolerant plants such as S. chilensis, which would explain its low population in this hostile environment.

Synthesis of Materials Based on La2O3 and SiO2 as Growth Stimulants for Salvia miltiorrhiza Bunge

In this paper, La2O3/SiO2 material was synthesized and applied for growth stimulants of Salvia miltiorrhiza Bunge. La2O3/SiO2 material was successfully synthesized from La(NO3)3 and SiO2 precursors by solid-phase thermal method. The material properties were confirmed through physicochemical characterization methods including XRD, EDX, IR, and SEM analysis. The results of using La2O3/SiO2 as a stimulating plant growth material for Salvia miltiorrhiza plants indicated that the use of La2O3/SiO2 on Salvia miltiorrhiza plants had a significant effect. In the experimental model, the dry root yield increased by 0.27 tons/hecta, the root length increased by 3.6 cm on average, and the harvest time for Salvia miltiorrhiza was eleven days earlier. Additionally, the Salvia miltiorrhiza with La2O3/SiO2 material was tougher than that without it, and gold leaf symptom was not observed in the experimental Salvia miltiorrhiza. Thus, the data suggested that La2O3/SiO2 had a favorable effect on alvia miltiorrhiza the harvest time for Salvia miltiorrhiza was eleven days.

EFFECT OF BARRIER DISCHARGE AT ATMOSPHERIC PRESSURE ON GERMINATION ENERGY AND GERMINATION OF COTTON VARIETY "FLORA" IN THE OPEN GROUND

In the conducted experiment, cotton seeds were treated with barrier discharges in air for 4 and 6 minutes. Barrier discharges are discharges in which the current flow is limited by the distinctive dimensions of at least one dielectric layer and electrode. It is noticeably larger than the size of the gap between the electrodes, which is a high pressure (more than many hundreds of Tor), spatial heterogeneity and short duration of different physical and chemical processes: the plasma BR is in strongly non-equilibrium criteria average temperature of electrons is a few electro-volts, and the temperature of the gas is close to the temperature of the dielectric barrier. This treatment is symmetric and sufficiently studied, since the electric field separation in the discharge zone can be considered uniform if the gap between the electrodes is rather narrow. Atmospheric pressure plasma is in demand for agriculture, stimulating dielectric, barrier discharge plasma at atmospheric pressure for 4 and 6 min irradiation to stimulate plant growth, germination energy and germination rate.

Foliar application of cyanobacterial formulations stimulates plant growth and fruit quality in tomato under protected cultivation

ABSTRACT Cyanobacterial biofertilizers enhance plant and soil productivity across crops, through soil-seed application at the field level; however, their use as foliar treatment is less investigated. This study evaluated the role of foliar sprays, initiated 4 weeks after transplantation of nursery-raised seedlings in beds, and effected at weekly intervals for a period of eight weeks. towards enhancing plant growth, yield and quality of tomato grown in a climate-controlled polyhouse. Sprays were composed of aqueous formulations of carrier alone, and those amended with the formulations of plant-growth-promoting cyanobacterium Anabaena laxa (A. laxa) or biofilm Anabaena torulosa -Trichoderma viride (An-Tr) or suspension of 100 µM Fe EDTA. A. laxa brought about a significant increase in nitrate reductase and glutamine synthetase activity in leaves by 13%–17%, along with 3-fold stimulation in carbonic anhydrase activity. Cyanobacterium-amended foliar agents significantly enhanced soil dehydrogenase, urease activity, along with increments in organic carbon, Fe, Zn in soil and stimulated yield, fresh weight and quality of fruits. Both cyanobacterial formulations were promising in enhancing plant growth, fruit yield and quality, however, A. laxa also significantly improved microbiological activity and nutrient enrichment in soil. Future research is focused towards optimising the dose and storage life of such foliar agents for protected horticulture.

Effect of the Pătârlagele Diatomite on Seed Germination and Growth of Sunflower Plants

Sunflower (Helianthus annuus L.) is the third largest agricultural crop in Romania, after corn and wheat. In 2021 Romania cultivated 1112 thousand ha of sunflower with a production of 2818 thousand tons, ranking first among EU countries both in terms of production and cultivated area. Diatomite, also called diatomaceous earth or kieselguhr, is a natural sedimentary rock with a high content of silicon dioxide and clay minerals, widely used in agriculture for its insecticidal and fungicidal properties, stimulating the process of seed germination and plant growth, as well as the ability to absorb and retain nutrients and water. The paper presents preliminary results regarding the effect of diatomite from Pătârlagele deposit (Buzău County) on seed germination and growth of young sunflower plants in laboratory conditions and on seeds production in the field at three sowing dates as well. A semi-early hybrid P64LE99 was used in experiments. Our data showed that the Pătârlagele diatomite improves sunflower seed performances both in laboratory and field conditions. In the laboratory, an increase in seed germination and stimulation of plant growth and development were observed in the diatomite treated samples compared to the untreated control. In the field under climatic and soil conditions of the Moara Domnească area (Southern Romania), the diatomite treated samples performed better than the control, both in terms of plant growth and development as well as production elements, in three sowing dates, middle and late April and early May. The highest yields were when sowing was in the first week of May for both variants.

Fungistatyczne właściwości siarki i miedzi – nowe perspektywy w ochronie roślin przed chorobami grzybowymi

New research brings promising findings on the fungistatic properties of sulfur and copper, opening up new perspectives in plant pro­tection against fungal diseases. Sulfur and copper, known for their properties that stimulate plant growth and development, also show intense activity against fungal pathogens. Their efficiency in diseases control makes them increasingly used in agricultural practice. How­ever, to maximize the potential of these substances, further on study their mechanisms of action and optimized application methods are required. In addition, scientists are looking for new, more effective fungistatic compounds that could be an alternative to traditional pesticides. Further research in this area could lead to the development of innovative crop protection strategies that are more effective, safer for the environment and more sustainable for agricultural production.

Enhancing Spinach growth and yield through the synergistic effects of Rhizobacter-Enriched compost

Plant Growth-Promoting Rhizobacteria (PGPR) and compost present promising solutions for bolstering agricultural sustainability by stimulating plant growth and yield while lessening dependence on chemical inputs. Our investigation delved into the combined effects of PGPR and compost on spinach cultivation, conducted at Amity University’s Organic Farm. Employing a Randomized Block Design (RBD) with six treatments and three replications, we evaluated how these treatments influenced spinach growth parameters and photosynthetic pigment levels. Results showed that treatment T5 (AIOA Strain + Compost) outperformed others, significantly boosting plant height, leaf breadth, root length, and leaf count per plant. Furthermore, T5 exhibited elevated chlorophyll and carotenoid levels, indicating enhanced photosynthetic function and overall plant vigor. These outcomes underscore the potential synergy between PGPR and compost in fostering sustainable crop production. By diminishing reliance on chemical fertilizers and pesticides while enhancing soil health and nutrient cycling, integrating PGPR and compost-based biofertilizers offers an economical and environmentally friendly avenue for augmenting agricultural productivity and mitigating environmental hazards. Further exploration into optimization techniques and underlying mechanisms is crucial to fully harness the potential of these microbial and organic interventions across varied agroecological settings. Overall, this study contributes to advancing our knowledge of employing microbial and organic methods in sustainable agriculture, crucial for addressing global challenges in food security and environmental conservation.

Effects of urban traffic noise on the early growth and transcription of Arabidopsis thaliana

Man-made noise in cities has been recognized as one of the major contributors to a range of adverse health outcomes, including heart disease. Besides the detrimental effects on human health, noise can act as a potential threat to other organisms in the ecosystem. Despite the growing concern, the biological effects of noise pollution on plants, in particular, remain largely obscure. Therefore, this study aimed to investigate the impacts of urban traffic noise on the growth of Arabidopsis thaliana (A. thaliana). We hypothesized that traffic noise could stimulate plant growth (seed germination and seedling growth) since noise treatment has been reported to improve immunity and drought tolerance in A. thaliana (1, 2). Seeds in the experimental group were exposed to 48 hours of traffic noise recorded in a highly congested area of Seoul, while the control group was exposed to background white noise. Compared with background white noise, traffic noise exposure had no effects on seed germination but significantly increased the growth of seedlings by 39%. We further investigated the traffic noise-induced molecular changes in the A. thaliana seedlings by employing RNA sequencing. We detected aberrant expression of 690 genes in traffic noise-exposed seedlings. The differentially expressed genes were enriched for various biological pathways, such as stress response and auxin signaling. The observed molecular changes suggest profound impacts of traffic noise on the physiology of A. thaliana. The results of our research can help shed light on the fundamental principles underlying the noise-plant interaction as well as provide a further basis for conserving our ecosystem.

Productivity of spring barley (Hordeum vulgare L.) with the complex use of biological preparations

The article reflects the results of studies conducted in the period from 2021 to 2023 in the dry steppe zone of the Russian Federation to assess the effectiveness of the integrated use of biological products in the cultivation of spring barley. The effectiveness of pre-sowing treatment of spring barley seeds with a biofungicide followed by foliar treatment during the tillering phase with biological products that suppress the development of phytopathogenic fungi and bacteria, stimulate plant growth and development, and also increase plant resistance to adverse environmental factors has been studied. Indicators of the prevalence and degree of development of root rot on spring barley were determined. Data are presented for recording the phytometric indicators of spring barley crops with the integrated use of biological products. Indicators of productivity and grain quality of spring barley were determined using biological products INBIO-FIT, BIOFIT-1.0 M and biofungicide Strekar

Influence of substrate and plant growth regulators on the rooting of Chrysanthemum

The purpose of the research is to study the influence of the substrate and plant growth stimulants on the rooting of cuttings of some varieties of Chrysanthemum coreanum (H. Lev. & Vaniot) Nakai garden group ‘Multiflora’. Four varieties of Chrysanthemum introduced into the South-Ural Botanical Garden-Institute of Ufa Federal Research Center of Russian Academy of Sciences (‘Sunbeam Bronze Bicolor’, ‘Meridian Red’, ‘Ditto Dark Pink’ and ‘Daybreak Apple Blossom’) were used as research objects. We examined the influence of two types of substrates (sand and vermiculite) and four growth stimulants on the rooting of chrysanthemums (Zircon, Lignohumate brand AM potassium, Beres Super seaweed extract, Beres AminoComplex). It was revealed that the treatment of chrysanthemum cuttings with plant growth regulators contributed to an increase in the number (by 7−117%) and length of roots (by 3−118%) in the majority of the studied objects. It was noted that positive results from the use of preparations were obtained when chrysanthemum cuttings were placed in vermiculite, in contrast to options with sand as a substrate. It has been shown that the greatest effect is noticeable when cuttings are soaked in Beres AminoComplex and Beres Super solutions compared to the control option. Varietal differences in chrysanthemums to the effects of root formation stimulants during vegetative propagation have been revealed. Thus, in the varieties ‘Ditto Dark Pink’ and ‘Daybreak Apple Blossom’, an improvement in the biometric parameters of cuttings was observed when rooted in vermiculite after exposure to plant growth regulators. It was noted that when chrysanthemum cuttings are soaked for three hours, in contrast to their wetting, the number of roots increases by 38−88% in all variants of the experiment.

Studying Stimulant and Fungicidal Properties of Preparation “Argitos Agro” against Powdery Mildew in Greenhouse Conditions

“Argitos Agro” is a plant growth stimulant with fungicidal effect and when disinfecting cucumber seeds and applying during the growing season, it does not have a significant effect on the development of powdery mildew disease. The preparation, indeed, has a stimulating effect on the germination of cucumber seeds, the growth of seedlings and plants, accelerates flowering, increases the yield and improves its quality indicators.