Step In Progression Research Articles

Dipsacoside B ameliorates cognitive impairment in sepsis-associated encephalopathy by reducing Th17 cell infiltration and neuroinflammation

Sepsis-associated encephalopathy (SAE) is the main cause of cognitive impairment in patients with sepsis. The infiltration of inflammatory signals into the central nervous system (CNS) via the compromised blood–brain barrier (BBB) represents a crucial step in the pathological progression of SAE. In particular, T-helper 17 cell (Th17 cells) has been suggested to be highly correlated with the activation of central immune responses. Thus, preventing Th17 cell infiltration into the CNS may be a possible strategy to alleviate cognitive decline in SAE. Dipsacoside B (DB) is one of the primary active components in Chuan Xu Duan (Dipsacus asper Wall). We speculate that DB may be a potential candidate for the treatment of SAE-related cognitive deficits. In the present study, we demonstrated that DB could effectively alleviate cognitive impairment in SAE mice. DB significantly suppressed the central inflammatory response induced by repeated lipopolysaccharide (LPS) injection. The mechanism underlying its therapeutic effect should be attributed to the reduction of BBB impairment and pathogenic Th17 cell infiltration into the CNS by inhibition of vascular endothelial growth factor A (VEGFA)/ Vascular endothelial growth factor receptor 2(VEGFR2)/ Endothelial nitric oxide synthase (eNOS) signaling. Our findings suggest that DB is a potential candidate for the treatment of SAE-related cognitive dysfunction.

GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene

GNA13 (Gα13) is one of two alpha subunit members of the G12/13 family of heterotrimeric G-proteins which mediate signaling downstream of GPCRs. It is known to be essential for embryonic development and vasculogenesis and has been increasingly shown to be involved in mediating several steps of cancer progression. Recent studies found that Gα13 can function as an oncogene and contributes to progression and metastasis of multiple tumor types, including ovarian, head and neck and prostate cancers. In most cases, Gα12 and Gα13, as closely related α-subunits in the subfamily, have similar cellular roles. However, in recent years their differences in signaling and function have started to emerge. We previously identified that Gα13 drives invasion of Triple Negative Breast Cancer (TNBC) cells in vitro. As a highly heterogenous disease with various well-defined molecular subtypes (ER+ /Her2−, ER+ /Her2+, Her2+, TNBC) and subtype associated outcomes, the function(s) of Gα13 beyond TNBC should be explored. Here, we report the finding that low expression of GNA13 is predictive of poorer survival in breast cancer, which challenges the conventional idea of Gα12/13 being universal oncogenes in solid tumors. Consistently, we found that Gα13 suppresses the proliferation in multiple ER+ breast cancer cell lines (MCF-7, ZR-75-1 and T47D). Loss of GNA13 expression drives cell proliferation, soft-agar colony formation and in vivo tumor formation in an orthotopic xenograft model. To evaluate the mechanism of Gα13 action, we performed RNA-sequencing analysis on these cell lines and found that loss of GNA13 results in the upregulation of MYC signaling pathways in ER+ breast cancer cells. Simultaneous silencing of MYC reversed the proliferative effect from the loss of GNA13, validating the role of MYC in Gα13 regulation of proliferation. Further, we found Gα13 regulates the expression of MYC, at both the transcript and protein level in an ERα dependent manner. Taken together, our study provides the first evidence for a tumor suppressive role for Gα13 in breast cancer cells and demonstrates for the first time the direct involvement of Gα13 in ER-dependent regulation of MYC signaling. With a few exceptions, elevated Gα13 levels are generally considered to be oncogenic, similar to Gα12. This study demonstrates an unexpected tumor suppressive role for Gα13 in ER+ breast cancer via regulation of MYC, suggesting that Gα13 can have subtype-dependent tumor suppressive roles in breast cancer.

Sustained Release Therapies with the Prostaglandin Analogues Intracameral Implants.

The travoprost intracameral implant was recently approved by the US Food and Drug Administration for sustained release medical treatment of open-angle glaucoma in the USA. The approval represents a substantial and progressive step forward in the area of sustained-release glaucoma therapy. Topical intraocular pressure-lowering medications for the treatment of glaucoma are faced with a host of challenges for long-term and usually lifelong care. A changing paradigm in glaucoma management involves first-line interventions with laser modalities, micro-invasive surgeries, and sustained-release treatment platforms. Future needs in the area of sustained-release therapy include a non-prostaglandin drug delivery platform and longer-term treatments that do not require surgical reintervention.

Coupled higher-order layerwise mechanics and finite element formulations for laminated composite beams with active SMA layers

This study proposes a thermo-elastic coupled nonlinear finite element (FE) model for laminated composite beams with active SMA layers based on a higher-order layerwise theory and Brinson's model of SMA's phase transformation constitutive relations. The developed FE Model is further discretized by using the Newton-Raphson time integration scheme to update SMA's phase transformation progress step by step. This new model can be used for thermal-mechanical behavior analysis of hybrid SMA-composite laminated beams, which especially enables accurate prediction for static response of moderately thick to thick beams, large deformation analysis, through-thickness variations of interlaminar stresses and strains. The accuracy and effectiveness of the proposed approach are verified by several numerical examples, and the results are compared with those obtained from corresponding alternative solutions and experimental tests. Using the developed FEM, the effects of temperature, geometrical nonlinearity, pre-strain of SMA, and stacking sequence of composite laminates on the static response of hybrid SMA-composite beams are studied.

ARTHROSCOPIC ANKLE JOINT AND ARTIFICIAL BONE GRAFTING FOR TREATMENT BONE CYSTS OF THE TALAR: A CASE REPORT.

ABSTRACTS Bone cysts of the talar body are a benign, rare condition. Remove bone cysts, autologous bone grafting is a treatment method many authors recognize. Approaching the calcaneal bone cysts of the talar body encounters difficulties due to the narrow surgical field, poor blood supply, and the entire surface of the talar body being covered with cartilage. Endoscopic talar joint surgery to remove cysts bone, autologous bone grafting in the treatment of calcaneal bone cysts of the talar body is a progressive step, with many advantages, overcoming the mentioned difficulties, albeit being a challenging technique. We report a case of bone cysts of the talar body treated with endoscopic ankle joint surgery to remove cysts bone and autologous bone grafting at Viet Duc University Hospital, with good postoperative results, and no recurrence after 8 months of follow-up.

Hybrid Learning: A combination of face-to-face and online learning

Background and Aims: Hybrid learning is important because it combines the flexibility of online education with the personal interaction of in-person classes, meeting a variety of learning needs and schedules. This approach increases engagement, offers a more personalized learning experience, and takes advantage of technological advancements to improve educational outcomes. Thus, the purpose of this paper is to investigate Hybrid Learning, which is a combination of in-person and online learning. Methodology: The review article used a comprehensive literature synthesis to investigate the effectiveness and limitations of hybrid learning models. By analyzing data from various studies, it identified best practices for integrating face-to-face and online instruction in educational settings. Results: The finding found that hybrid learning, which combines face-to-face and online instruction, provides a flexible and inclusive educational approach that accommodates a wide range of student needs and preferences. By combining the immediate feedback and personal engagement of in-person classes with the accessibility and adaptability of online learning, hybrid models create a rich and dynamic learning environment. This combination not only improves student engagement and outcomes but also enables educators to employ a wide range of teaching methods and technologies. Finally, hybrid learning represents a progressive step toward more personalized and effective education, meeting the needs of both modern learners and educators. Conclusion: Hybrid learning effectively combines in-person and online instruction, resulting in a flexible educational environment that meets the diverse needs of students. This approach improves engagement and outcomes, paving the way for more personalized and effective learning experiences in today's educational environment.

Qurio-City: Seeking Refuge in Unconventional Habitats

The project aims to design a regenerative and resilient space centre for urban environments. It explores theconcept of architecture as a responsive machine, adapting to urban, socio-economic and environmentaldynamics. Drawing inspiration from natural systems, the project involves creating three-dimensional prototypesto analyse ecological infrastructure. In a unique approach, the exploration extends to developing chainprototypes inspired by the collaborative and efficient behaviours observed in weaver ants, further enriching theproject's understanding of interconnected ecological systems. These assemblies contribute to the developmentof a new architectural paradigm that dynamically responds to the ever-changing environment. The envisionedspace centre, with its human-centric design, sustainable features, and adaptable technology, becomes atransformative hub within the urban landscape. It is poised to integrate new space exploration technologies,adjust to changes, and symbolise a progressive step towards emergent ecological morphology in architecture.

Adoption of an evidence-based practice approach in the child and family care system in Europe: A comparative survey study

The European policy is placing a strong emphasis onadopting an evidence-based practice (EBP) approach. However, this process has not been sufficiently described and comparatively explored between countries with different family policies, delivery systems, target populations and professional cultures of evaluation and intervention. This study proposed a multicomponent model of implementation strategies to empirically examine the degree of adoption of EBP at national, agency, and practitioner levels in a sample of20 member countries of the European family support network: A bottom-up, evidence-based and multidisciplinary approach (EurofamNet), with a great geographical distribution and different political status: 12 EU members, 6 EU candidate members and 2 non-EU members. Consensual responses to the survey (17 questions) were provided by members of EurofamNet national networks made up of entities at the national, regional, and local levels across sectors. Three clusters of responses were identified, Cluster 1 Initial Level (30%, mainly EU candidates), Cluster 2 Medium Level (40%, EU and non-EU members) and Cluster 3 Advance level (30%, EU and non-EU members). Countries at the Initial Level showed a lack of / or less degree of achievement in the implementation strategies. Countries at the Medium and Advance levels showed progressive steps towards EBP adoption through the implementation of collaborative leadership, translational efforts on agreed EBP guidelines, official recognition of services and professional teams applying EBP guidelines, multi-agency and intersectoral EBP adoption, professional training on EBP guidelines, and dissemination strategies. Our findings highlight the complexity of the transformation process and the multiple determinants involved in bringing the quality standards into the practice environment. Lines of improvement are suggested to help countries reinforce the culture of quality assurance in the child and family services.

Peluang dan Tantangan Implementasi Kurikulum Merdeka Belajar: Sebuah Studi Interview di Sekolah Penggerak dan Mandiri Berubah

The implementation of the Merdeka Belajar curriculum in State Elementary Schools (SES) in Kendari city marks a progressive step in adapting education to contemporary needs. This study aims to understand the implementation of this curriculum in SES Kendari city and identify the challenges and opportunities associated with its implementation. The research employs a qualitative approach through interviews with seven informants, including teachers and school principals from SES involved in the evolving independent school program. Interview data are analyzed through transcription, coding, and reflective analysis. The findings indicate that despite facing challenges such as teacher readiness and resource availability, the Merdeka Belajar curriculum has a positive impact on increasing student engagement and teacher creativity. Support from the Department of Education also emerges as a key factor in the successful implementation. Therefore, this research provides a deep understanding of the implementation of the Merdeka Belajar curriculum in SES Kendari city and highlights the importance of enhancing support and implementation strategies to achieve more inclusive and responsive educational goals.

Tracing the path of cycling initiatives: Revisiting Lancaster's CDT program and unravelling current policies

IntroductionActive modes of transportation have gained significant prominence in many cities, with governments and public health organizations increasingly advocating for cycling. While policy and planning decisions are actively aimed at boosting cycling rates, their long-term impact remains somewhat uncertain. This study seeks to shed light on the enduring effects of policy and planning decisions on cycling and outlines potential steps for sustainable progress. MethodsTo achieve this, we have chosen Lancaster, UK, as our case study, with a specific focus on the UK Government-funded Cycling Demonstration Towns (CDTs) program that operated from 2005 to 2011. Our approach involves revisiting this program and the changes in the city through a multi-pronged methodology: conducting surveys with cyclists (n = 65), engaging in interviews with personnel from Lancaster City and Lancashire County Council (n = 3), and analyzing cyclist numbers at specific locations (n = 5). ResultsThe findings of our study reveal the positive influence of the CDTs program on cycling rates and the perspectives of cyclists in Lancaster. However, after the program's completion, progress has been somewhat gradual, drawing attention to specific areas in need of improvement, particularly infrastructure and safety measures. ConclusionsIn conclusion, we delve into potential strategies for implementation, not only within Lancaster but also for other CDTs and cities aspiring to promote cycling initiatives. This underscores the effectiveness of such programs and decisions in the short-term while emphasizing the critical importance of sustained investments in infrastructure and maintenance for long-term impact.

Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells.

Non-homologous end joining (NHEJ) is the predominant pathway that repairs DNA double-stranded breaks (DSBs) in vertebrates. However, due to challenges in detecting DSBs in living cells, the repair capacity of the NHEJ pathway is unknown. The DNA termini of many DSBs must be processed to allow ligation while minimizing genetic changes that result from break repair. Emerging models propose that DNA termini are first synapsed ~115Å apart in one of several long-range synaptic complexes before transitioning into a short-range synaptic complex that juxtaposes DNA ends to facilitate ligation. The transition from long-range to short-range synaptic complexes involves both conformational and compositional changes of the NHEJ factors bound to the DNA break. Importantly, it is unclear how NHEJ proceeds in vivo because of the challenges involved in analyzing recruitment of NHEJ factors to DSBs over time in living cells. Here, we develop a new approach to study the temporal and compositional dynamics of NHEJ complexes using live cell single-molecule imaging. Our results provide direct evidence for stepwise maturation of the NHEJ complex, pinpoint key regulatory steps in NHEJ progression, and define the overall repair capacity NHEJ in living cells.

Predictive value of preoperative CT enhancement rate and CT perfusion parameters in colorectal cancer

BackgroundAngiogenesis is a critical step in colorectal cancer growth, progression and metastasization. CT are routine imaging examinations for preoperative clinical evaluation in colorectal cancer patients. This study aimed to investigate the predictive value of preoperative CT enhancement rate (CER) and CT perfusion parameters on angiogenesis in colorectal cancer, as well as the association of preoperative CER and CT perfusion parameters with serum markers.MethodsThis retrospective analysis included 42 patients with colorectal adenocarcinoma. Median of microvessel density (MVD) as the cut-off value, it divided 42 patients into high-density group (MVD ≥ 35/field, n = 24) and low-density group (MVD < 35/field, n = 18), and 25 patients with benign colorectal lesions were collected as the control group. Statistical analysis of CER, CT perfusion parameters, serum markers were performed in all groups. Receiver operating curves (ROC) were plotted to evaluate the diagnostic efficacy of relevant CT perfusion parameters for tumor angiogenesis; Pearson correlation analysis explored potential association between CER, CT perfusion parameters and serum markers.ResultsCER, blood volume (BV), blood flow (BF), permeability surface (PS) and carbohydrate antigen 19 − 9 (CA19-9), carbohydrate antigen 125 (CA125), carcinoembryonic antigen (CEA), trefoil factor 3 (TFF3), vascular endothelial growth factor (VEGF) in colorectal adenocarcinoma were significantly higher than those in the control group, the parameters in high-density group were significantly higher than those in the low-density group (P < 0.05); however, the time to peak (TTP) of patients in colorectal adenocarcinoma were significantly lower than those in the control group, and the high-density group showed a significantly lower level compared to the low-density group (P < 0.05). The combined parameters BF + TTP + PS and BV + BF + TTP + PS demonstrated the highest area under the curve (AUC), both at 0.991. Pearson correlation analysis showed that the serum levels of CA19-9, CA125, CEA, TFF3, and VEGF in patients showed positive correlations with CER, BV, BF, and PS (P < 0.05), while these indicators exhibited negative correlations with TTP (P < 0.05).ConclusionsSome single and joint preoperative CT perfusion parameters can accurately predict tumor angiogenesis in colorectal adenocarcinoma. Preoperative CER and CT perfusion parameters have certain association with serum markers.

Panoramic RNA expression of fibroblast growth factors in human glioblastoma tissues and the impact on the survival of patients.

Fibroblast growth factors (FGFs) have a key role in various critical steps of tumor growth and progression through effects on angiogenesis, inflammation and the growth and invasion of malignant cells. Nevertheless, the role of the FGF family in human glioblastoma (GBM) has been rarely studied. The objective of the present study was to assess the RNA expression of all FGF family members in tissues obtained from patients with GBM and to analyze the association between FGF expression and the survival of these patients. For this, the RNA expression of FGF family members in the malignant and proximal tissues of 12 patients with GBM was determined by analyzing high-throughput RNA transcriptome sequencing data uploaded to the National Center for Biotechnology Information database. The relationship between FGF genes and the survival of patients with GBM and glioma was also respectively studied by analyzing data from The Cancer Genome Atlas database using the Gene Expression Profiling Interactive Analysis tool. The results showed that the expression of FGF1, FGF17, FGF20 and FGF22 in GBM tissues was lower than that in adjacent tissues, with a difference of >2 times. Analysis of the overall survival of patients with GBM indicated there were no significant relationships between the expression of FGF1, FGF17, FGF20, FGF22 and overall survival. Analysis of the overall survival of patients with glioma showed that glioma patients with low FGF1 expression achieved a longer survival time than patients with high FGF1 expression; however, high expression of FGF17 and FGF22 indicated a longer survival time. In summary, the results of the present study demonstrated the panoramic expression of FGF family members in patients with GBM, and indicated that FGF1, FGF17 and FGF22 did not affect the survival of patients with GBM, but had a notable influence on the survival of patients with glioma.

Community Education and General Elections: Strategies to Enhance Political Awareness Based on Technology &amp; Community Participation Levels in Gorontalo Province

Community participation serves as a measure of success in conducting general elections, thus political participation must be optimized through building and developing political awareness among the populace through various efforts, including education and political advocacy based on information technology. This study aims to design a strategy to enhance technology-based political awareness and community participation levels in Gorontalo Province. The method employed in this study is qualitative, with data analysis utilizing the Miles and Huberman model. The study findings reveal that community political participation in local elections in Gorontalo Province has reached a satisfactory level, although there is still room for improvement. Community education on politics with a technology-based approach is also crucial, especially in addressing digital divides. By involving technology, political education can become more accessible to the populace. It is hoped that political participation will increase, voters will be better educated, and local democracy will become stronger. Communities engaged in politics have greater potential to influence policies, monitor the government, and uphold accountability. Technology-based political education is a progressive step towards achieving the goal of community awareness and participation in general elections. Keywords: Community Education, Elections, Awareness, Participation.

Implementasi Platform Edukasi Pada Klinik Medikasih Pondok Kelapa Jakarta Timur

Medikasih Clinic, which is located in Pondok Kelapa Village, Duren Sawit District, East Jakarta City, Special Capital Region of Jakarta, currently relies on a paid platform to manage clinic operations. However, there is no specific health education platform available for patients/customers, and the importance of improving the quality of health services at this clinic is highly recognized. To overcome these challenges, the service team plans to launch a web-based health education platform. This platform aims to support increased patient participation and health literacy by presenting health information from various medical sources. Based on the analysis of popular medical record diagnoses at Medikasih Clinic, we are determined to provide users with easy and reliable access to understanding health conditions, preventive measures, and healthy lifestyle practices. In conclusion, the development of this platform is a progressive step to create a positive impact. A focus on increasing health literacy and patient participation will create a society that is more informed and involved in personal health management. Our recommendation is to engage health experts and specialists to contribute educational content, ensuring the accuracy and reliability of the information presented

DNA-Binding Proteins and Passenger Proteins in Plasma DNA-Protein Complexes: Imprint of Parental Cells or Key Mediators of Carcinogenesis Processes?

Knowledge of the composition of proteins that interact with plasma DNA will provide a better understanding of the homeostasis of circulating nucleic acids and the various modes of interaction with target cells, which may be useful in the development of gene targeted therapy approaches. The goal of the present study is to shed light on the composition and architecture of histone-containing nucleoprotein complexes (NPCs) from the blood plasma of healthy females (HFs) and breast cancer patients (BCPs) and to explore the relationship of proteins with crucial steps of tumor progression: epithelial-mesenchymal transition (EMT), cell proliferation, invasion, cell migration, stimulation of angiogenesis, and immune response. MALDI-TOF mass spectrometric analysis of NPCs isolated from blood samples using affine chromatography was performed. Bioinformatics analysis showed that the shares of DNA-binding proteins in the compositions of NPCs in normal and cancer patients are comparable and amount to 40% and 33%, respectively; in total, we identified 38 types of DNA-binding motifs. Functional enrichment analysis using FunRich 3.13 showed that, in BCP blood, the share of DNA-binding proteins involved in nucleic acid metabolism increased, while the proportion of proteins involved in intercellular communication and signal transduction decreased. The representation of NPC passenger proteins in breast cancer also changes: the proportion of proteins involved in transport increases and the share of proteins involved in energy biological pathways decreases. Moreover, in the HF blood, proteins involved in the processes of apoptosis were more represented in the composition of NPCs and in the BCP blood-in the processes of active secretion. For the first time, bioinformatics approaches were used to visualize the architecture of circulating NPCs in the blood and to show that breast cancer has an increased representation of passenger proteins involved in EMT, cell proliferation, invasion, cell migration, and immune response. Using breast cancer protein data from the Human Protein Atlas (HPA) and DEPC, we found that 86% of NPC proteins in the blood of BCPs were not previously annotated in these databases. The obtained data may indirectly indicate directed protein sorting in NPCs, which, along with extracellular vesicles, can not only be diagnostically significant molecules for liquid biopsy, but can also carry out the directed transfer of genetic material from donor cells to recipient cells.

Optimizing English education curriculum for EFL students: Multimodal approach in academic writing

English education faces challenges in academic writing due to technological advancements and multimodal writing. Due to the incompatibility between present curricula and student demands, the study intends to restructure the curriculum to emphasize multimodal writing projects that meet students' needs and multimodal paradigms. The study was a type of Design and Development Research using the ADDIE (Analysis, Design, Development, Implementation, and Evaluation) model, specifically the Analysis and Design stages. It involved 30 students and three team-teaching lecturers of the English Education Study Program at a private university in Indonesia. The data collection is conducted through a content analysis of the existing syllabus, needs assessment using questionnaires to the students, followed by interviews with the lecturers to further reinforce the data obtained from the questionnaires. Data analysis was conducted using a qualitative methodology that incorporates triangulation techniques and relationship mapping. The research results highlighted the need for curricula that are more tailored to multimodal projects and student needs. It implies that curriculum reconstruction has become crucial to accommodating changing needs and technologies. It is a progressive step in improving the quality of English language education.

Antitrust Law in the BRICS Countries: A Review and Critique

A growing trend shows that global economic power is shifting away from the United States and Europe and towards the BRICS nations. The BRICS nations are predicted to hold the dominant position in the world by 2050. The rapid development of these economies and their markets is also raising a serious concern for the national antitrust enforcement authorities of the BRICS countries in their respective jurisdictions. This study attempts to examine the effectiveness of antitrust law, with a special focus on new developing trends in each of the BRICS jurisdictions. In addition, the study examines the proposed changes and limitations of the Indian Competition (Amendment) Bill 2022. The study reveals that the national antitrust laws and their enforcement processes are not fully developed and in need of considerable changes. Furthermore, the study indicates that while the proposed Amendment Bill 2022 is a progressive step in the right direction, it fails to cover certain key areas of the digital era, requiring further modifications to anticipate future impacts of economic development. In conclusion, the author recommends some points for effective policymaking, along with their future implications.

Navigating the journey as a peer reviewer: A roadmap to success

Peer review is an essential step in scientific progress and clinical improvement, providing opportunity for research to be critically evaluated and improved by one's colleagues. Pharmacists from all job settings are called to serve as peer reviewers in the ever-growing publication landscape of the profession. Despite challenges to engagement such as time and compensation, peer review provides considerable professional development for both authors and reviewers alike. This article will serve as a practical guide for peer reviewers, discussing best practices as well as the handling of different situations that may arise during the process.

Abstract PO5-06-12: Genetic screen identifies epigenetic drivers of breast cancer brain metastasis

Abstract Metastatic breast cancer remains a major cause of cancer-related deaths in women, and there are few effective therapies against this advanced disease. Brain metastases, found in 15~35% of patients with breast cancer, are often incurable. Accumulating evidence suggests that key steps of tumor progression and metastasis are controlled by reversible epigenetic mechanisms. To systematically identify the druggable epigenetic regulators essential for breast cancer brain metastasis, we conducted an in vivo shRNA screen and identified multiple epigenetic regulators, including RNA acetyltransferase NAT10 as drivers of breast cancer brain metastasis. Knockdown of NAT10 significantly restrains breast cancer cell proliferation and migration in vitro and brain metastases in vivo. NAT10 promotes breast cancer cell growth through its RNA helicase activity but not its acetyltransferase activity. Integrative analyses of transcriptomics, proteomics, and NAT10 eCLIP-Seq data identify NAT10 downstream targets critical for breast cancer progression and metastasis. Thus, these results reveal novel therapeutic strategies to treat metastatic breast cancer. Citation Format: Jocelyn Chen, Wesley Cai, Peng Xu, Don Nguyen, Qin Yan. Genetic screen identifies epigenetic drivers of breast cancer brain metastasis [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO5-06-12.