The steel–concrete composite beam, as a structural form that combines the advantages of steel and concrete, has been applied in railway engineering. However, with the increase in railway operation time, the degradation pattern of the service performance of steel–concrete composite bridges remains unclear. This paper proposes a method for calculating the long-term service performance of railway steel–concrete composite beams, considering the cumulative fatigue damage of steel beams and studs, based on the vehicle–bridge coupling theory and Miner’s linear cumulative damage criterion. The proposed method is validated using measured data from an in-service steel–concrete composite railway bridge with spans of 40 + 50 + 40 m. The calculated mid-span vertical displacement and the first two natural frequencies of the composite beam deviated from the measured results by only 2.1%, 7.7%, and 9.5%, respectively. The research results can provide a basis for extending the service life of composite beams and preventing the occurrence of safety accidents.
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