This paper presents a novel proposal for an assembled bolted joint between precast reinforced concrete (RC) column and steel-concrete composite beams, focusing on its seismic performance. To evaluate the joint's behavior, a comprehensive experimental investigation was conducted on a full-scale RC column to composite beam joint specimen subjected to pseudo-static loading. The study aimed to analyze the joint's failure mode, bearing capacity, ductility, and energy dissipation characteristics. The joint stiffness was assessed by analyzing the bending moment-rotation (M-θ) curve of the beam in accordance with the European standard BS EN 1993-1-8:2005. The experimental results demonstrate that the proposed assembled bolted joint exhibits semi-rigid behavior under positive moments and approaches rigidity under negative moments. To further enhance our understanding of the joint's performance, a finite element model was developed using ABAQUS. The stress distribution in each joint component, as well as the failure mode and ultimate bearing capacity, were analyzed in conjunction with the experimental results. Based on the findings from both the experimental and finite element analyses, an improved joint design is proposed. Additionally, parametric analysis was conducted on the enhanced joint configuration. The study findings indicate that the improved joint exhibits superior damage resistance, seismic performance, and enhanced assembly efficiency compared to the original design. This research contributes to the advancement of assembled bolted joints between precast RC columns and steel-concrete composite beams, offering valuable insights for practical applications in seismic regions.