Status Of Cattle Research Articles

Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.

The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds. First, we collected blood from 3 cattle herds and, based on the measurement of BLV-proviral load (PVL) by quantitative real-time PCR (qPCR), identified 1) a BLV-free herd, 2) a herd with a low prevalence of BLV-infected cattle and low PVL, and 3) a herd wherein half of the cattle were BLV-infected with low-to-high PVLs. Next, we collected stable flies from the 3 herds, extracted DNA from their blood meals, analyzed it for BLV DNA, and measured the BLV PVL. Cattle DNA and BLV DNA, but not other mammalian DNA, were successfully detected by digestion of the flies. Based on fly blood meal qPCR, we identified one herd as BLV-free and the other 2 herds as having <50% prevalence of BLV-infected cattle with low PVLs. Our fly results were not consistent with preliminary BLV-PVL measurements on cattle blood. Our pilot study indicated that, to assess the feasibility of a stable fly blood meal test as an alternative technique for evaluating BLV infection status in dairy and beef cattle, additional investigations involving more cattle herds and stable flies are needed.

Early detection of pregnancy status in cattle using ISG17 polyclonal antibody recombinant

Abstract Optimization of female cattle reproduction requires reliable and accurate systems for early pregnancy detection. Identifying pregnant status as early as possible is essential in managing reproductive cycles because it allows farmers to make early decisions about rebreeding strategies. Interferon stimulated gen (ISG) 17 is a gene expressed during maternal recognition pregnancy through Interferon tau (IFN-T) secretion signal, which begins on days 8 to 9 of pregnancy and peaks on days 16 to 17 (day 0 = day of estrus). This study aims to detect ISG17 expression using ISG17 polyclonal antibodies as a determinant of pregnancy in cattle that have received artificial insemination. The polyclonal antibody of ISG17 was produced in New Zealand white rabbits using ISG17 recombinant protein expressed by pET-32b expression vector in Escherichia coli systems of strain pLys BL21-Origami. ISG17 polyclonal antibody (ISG17-pAb) was used to detect ISG17 proteins expressed in blood serum obtained from 2 to 10 weeks-inseminated cattle (Frisian Holstein and Ongole) by NCM-ELISA method. ISG17-pAb was also labeled with gold nanoparticles and assembled into a lateral flow system for easier pregnancy detection in the field. NCM-ELISA test results showed a typical reaction between ISG17 antigen and ISG17-pAb in the form of brown dots after adding DAB substrate. Detection of pregnancy status showed that the antibody reaction to blood serum from dairy cows indicated 13.2% false negatives, 15.8% false positives, and 71.0% accuracy. In comparison, blood serum from beef cattle showed 7.5% false negatives, 0% false positives, and 92.5% accuracy. Interestingly, a lateral flow kit based on ISG17-pAb-gold nanoparticles showed good antibody reactions against blood serum from pregnant and non-pregnant cows. This indicates that the development of early pregnancy detection based on ISG17-pAb is prospective for optimizing productive cows by continuing to increase accuracy.

Bovine TB infection status in cattle in Great Britain in 2022.

Bovine TB infection status in cattle in Great Britain in 2022.

Evidence for and against manganese deficiency as causal for congenital joint deficiency disease or death in fetal and neonatal cattle.

Measures of manganese (Mn) status in cattle vary among studies, and no single criterion accurately predicts or diagnoses Mn deficiency and pathologic outcomes. Mn deficiency causes congenital joint laxity and dwarfism (CJLD) when total dietary intake is <20 ppm Mn dry matter (DM) for most of the pregnancy. However, the recommended dietary intake of 40 ppm DM can also result in clinical Mn deficiency. Some studies have found that CJLD occurs in calves from cows fed red clover or silage but not in calves from cows fed hay. The concentration of Mn in the liver is the best indicator of Mn status in neonates and adults but cannot be interpreted in fetuses. Serum, plasma, and whole blood concentrations of Mn are unreliable indicators of bovine Mn status. The primary objective of our report is to present evidence linking CJLD to a primary or secondary Mn deficiency. To predict and diagnose Mn deficiency in cattle, we propose using a combination of clinical signs, dietary Mn, liver Mn at birth and beyond, positive response to Mn supplementation or the replacement of silage with other forages, and ruling out other causes of malformations. By following these recommendations, we expect that CJLD and gestational death will decrease as hepatic Mn concentrations increase at birth. Many publications we reviewed are not statistically sound, and future research should include a statistician from the initial discussions of the study through the final publication.

Differences in the intestinal microbiota and association of host metabolism with hair coat status in cattle.

The hair coat status of cattle serves as an easily observed indicator of economic value in livestock production; however, the underlying mechanism remains largely unknown. Therefore, the objective of the current study was to determine differences in the intestinal microbiota and metabolome of cattle based on a division of with either slick and shining (SHC) or rough and dull (MHC) hair coat in Simmental cows. Eight SHC and eight MHC late-pregnancy Simmental cows (with similar parities, body weights, and body conditions) were selected based on their hair coat status, and blood samples (plasma) from coccygeal venipuncture and fecal samples from the rectum were collected. The intestinal microbiota (in the fecal samples) was characterized by employing 16S rRNA gene sequencing targeting the V3-V4 hypervariable region on the Illumina MiSeq PE300 platform, and plasma samples were subjected to LC-MS/MS-based metabolomics with Progenesis QI 2.3. Plasma macromolecular metabolites were examined for differences in the metabolism of lipids, proteins, mineral elements, and hormones. Notable differences between the SHC and MHC groups related to host hair coat status were observed in the host metabolome and intestinal microbiota (P < 0.05). The host metabolome was enriched in histidine metabolism, cysteine and methionine metabolism, and purine metabolism in the SHC group, and the intestinal microbiota were also enriched in histidine metabolism (P < 0.05). In the MHC group, the symbiotic relationship transitioned from cooperation to competition in the MHC group, and an uncoupling effect was present in the microbe-metabolite association of intestine microbiota-host interactions. The hubs mediating the relationships between intestinal microbiota and plasma metabolites were the intestinal bacterial genus g__norank_f__Eubacterium_coprostanoligenes_group, plasma inosine, triiodothyronine, and phosphorus, which could be used to differentiate cows' hair coat status (P < 0.05). Overall, the present study identified the relationships between the features of the intestinal microbiota and host hair coat status, thereby providing evidence and a new direction (intestine microbiota-host interplay) for future studies aimed at understanding the hair coat status of cattle.

Assessment of serum calprotectin as a marker ofinflammation in cattle with traumatic reticuloperitonitis.

Calprotectin (CP) is an inflammatory marker. The aim of the current study was to investigate oxidative stress and changes in CP in cattle with traumatic reticuloperitonitis (TRP). The study was divided into two groups, experimental (TRP) and healthy control group, with 10 animals in each group. Total leucocyte count, neutrophil and lymphocyte counts were higher in the TRP group compared to the control group and this increase was statistically significant (P < 0.001). The level of malondialdehyde (MDA) in TRP group was statistically significantly higher than the control group (P < 0.001). The level of glutathione (GSH) in the TRP group was statistically significantly lower than in the control group (P< 0.001). Serum Amyloid A (SAA) and CP values were higher in the TRP group and this difference was statistically significant (P < 0.001). It was concluded as a result of ROC analysis that CP, which has similar values with SAA, can be used diagnostically to confirm the inflammatory status in cattle with TRP.

Detection of anti-Trypanosoma spp. antibodies in cattle from southern Brazil.

Bovine trypanosomosis, caused by Trypanosoma vivax, is a disease that originated in Africa and currently affects cattle in several South American countries, including almost all Brazilian states. Despite the reports on T. vivax infection in southern Brazil, data on its circulation status is currently unavailable. In this study, we aimed to detect anti-Trypanosoma spp. IgG antibodies in cattle from Rio Grande do Sul and suggest areas with T. vivax transmission risk. A total of 691 serum samples from cattle in the intermediate regions of Rio Grande do Sul were analyzed using indirect immunofluorescence assay (IFA). The overall seroprevalence of anti-Trypanosoma antibodies in cattle was 24.6% (170/691). The detection rate ranged from 0-37.3%, with a high prevalence in the intermediate regions of Ijuí (37.3%), Uruguaiana (30.7%), and Passo Fundo (28.9%). Thus, these regions were suggested as possible bovine trypanosomosis risk areas due to the high seroprevalence. This is the first serological study to determine Trypanosoma spp. infection status in cattle from Rio Grande do Sul, providing data on the epidemiology of trypanosomosis in the state.

166 Influence of Source of Metabolizable Protein and Implant Status on Feedlot Performance and Feeding Behavior in Yearling Steers

Abstract Crossbred steers (n = 60; beginning BW 454 ± 24 kg) were used in a split-plot design with a 2 x 3 factorial arrangement of treatments to determine effects of source of metabolizable AA and implant status on animal performance and feeding behavior. Calves were blocked by initial BW and assigned to 1 of 5 BW blocks. Pen (n = 2 pens, 6 steer/pen) within each BW block were fed diets formulated to meet the RDP requirement of cattle and provide 150% and 152% the metabolizable Lys and Met requirement (RP-AA), or a diet designed to meet RDP and closely match the predicted metabolizable Lys and Met requirement (RDP-ONLY; metabolizable Lys = 99.3% of required; metabolizable Met = 119% of required, NASEM, 2016). Subsequently, within pen two calves were randomly assigned to 1 of 3 implant strategies: no implant, moderate (100 mg trenbolone acetate and 14 mg estradiol; Synovex Choice) or potent (200 mg trenbolone acetate and 28 mg estradiol; Synovex Plus). Cattle were fed for 125 days and feed intake and feeding behavior was determined using a semi-autonomous feed monitoring system (GrowSafe System Ltd). Data were analyzed using the MIXED procedure of SAS. There was a tendency for an interaction of source of metabolizable AA and implant status on dry matter intake; calves with a moderate implant consumed less feed when fed RP-AA while calves with a potent implant consumed greater amounts. Dry matter intake was not affected by metabolizable AA supply (P = 0.87) or implant status (P = 0.43). There was an interaction of source of metabolizable AA and implant status on ADG; implanted calves gained similar amount regardless of source of metabolizable AA, but non-implanted calves fed RP-AA gained 25% less compared with RDP-ONLY diet. Moderate and potent implants had greater ADG (P &amp;lt; 0.01) than non-implanted cattle. Overall, ADG was not affected by source of metabolizable AA (P=0.95). Feeding behavior was largely unaffected by source of metabolizable AA or implant status. Feed intake per meal, number of meals per day, meal duration and visit duration were not influenced by source of metabolizable AA or implant status (P ≥ 0.16). Number of visits per meal tended (P = 0.07) to be greater for RP-AA while feeding rate tended (P = 0.09) to be less. There was a significant interaction of source of metabolizable AA and implant status on feeding rate (P &amp;lt; 0.01) as non-implanted and potently implanted calves ate feed at a lesser rate when fed RP-AA but moderately implanted calves fed RP-AA ate feed at a greater rate compared with RDP-ONLY. These data demonstrate that metabolizable AA requirements of growing cattle are dynamically related to implant status in cattle, but that implant status does not largely influence feeding behavior.

SWATH-MS Analysis of Blood Plasma and Circulating Small Extracellular Vesicles Enables Detection of Putative Protein Biomarkers of Fertility in Young and Aged Dairy Cows.

The development of biomarkers of fertility could provide benefits for the genetic improvement of dairy cows. Circulating small extracellular vesicles (sEVs) show promise as diagnostic or prognostic markers since their cargo reflects the metabolic state of the cell of origin; thus, they mirror the physiological status of the host. Here, we employed data-independent acquisition mass spectrometry to survey the plasma and plasma sEV proteomes of two different cohorts of Young (Peripubertal; n = 30) and Aged (Primiparous; n = 20) dairy cows (Bos taurus) of high- and low-genetic merit of fertility and known pregnancy outcomes (ProteomeXchange data set identifier PXD042891). We established predictive models of fertility status with an area under the curve of 0.97 (sEV; p value = 3.302e-07) and 0.95 (plasma; p value = 6.405e-08). Biomarker candidates unique to high-fertility Young cattle had a sensitivity of 0.77 and specificity of 0.67 (*p = 0.0287). Low-fertility biomarker candidates uniquely identified in sEVs from Young and Aged cattle had a sensitivity and specificity of 0.69 and 1.0, respectively (***p = 0.0005). Our bioinformatics pipeline enabled quantification of plasma and circulating sEV proteins associated with fertility phenotype. Further investigations are warranted to validate this research in a larger population, which may lead to improved classification of fertility status in cattle.

Bovine TB infection status in cattle in Great Britain in 2021.

Bovine TB infection status in cattle in Great Britain in 2021.

Evaluation of erythrocyte arginase activity, plasma nitric oxide concentration and oxidative stress status in cattle with anaplasmosis

Bovine anaplasmosis is an arthropod-borne disease characterized by high fever, anaemia and sometimes jaundice. The role of oxidative stress in anaplasmosis has been investigated, but erythrocyte arginase (ARG) activity has not been studied. In this study, we aimed to investigate the changes in haematological parameters, erythrocyte ARG activity, plasma nitric oxide (NO) levels and oxidative stress parameters and explain the relationship between each other in cattle with anaplasmosis. The material of this study consisted of 14 cattle, aged 10–12 months with anaplasmosis (infected group) and 14 healthy cattle aged 10–12 months (control group). Our data revealed that leukocyte parameters and plasma NO levels and serum malondialdehyde (MDA), total oxidant status (TOS) and oxidative stress index (OSI) levels were higher while erythrocyte parameters, erythrocyte ARG activity and serum total antioxidant status (TAS) and glutathione (GSH) levels were lower in the infected group compared to the control group. There was a strong correlation between erythrocyte ARG activity and NO, MDA, TOS, OSI, TAS and GSH. ROC analysis and correlation results suggest that erythrocyte ARG activity is an effective oxidative stress marker. We concluded that severe oxidative stress occurs in anaplasmosis. As the severity of anaemia increases, erythrocyte ARG activity plummets while plasma NO level elevates. These two parameters may also be used as prognostic and oxidative stress markers. Although decreased erythrocyte ARG activity is a disadvantage in haemolytic diseases, this situation can be compensated by increased NO. Thus, homeostasis of these two parameters may contribute to the elimination of the infection.

Bovine TB infection status in cattle in Great Britain in 2020.

Bovine TB infection status in cattle in Great Britain in 2020.

Assessment of oxidative stress and antioxidant status in cattle infected with Theileria orientalis

Oriental theileriosis is a major haemoprotozoan disease of cattle and causes huge economic losses to the farmers. Oxidative stress plays an important role in the development of anaemia and subsequent complications associated with theileriosis. The present study was conducted to assess oxidative stress and antioxidant status of cattle infected with Theileria orientalis. In the present study, 16 animals which were positive for theileriosis by blood smear examination were selected. Confirmation of oriental theileriosis was done with polymerase chain reaction (PCR). Assessment of oxidative stress and antioxidant status were done by measuring malondialdehyde (MDA) levels and reduced glutathione activity respectively. Both MDA levels and reduced glutathione activity did not indicate any significant difference in animals infected with T. orientalis from control animals.

Estatus proteico en Bovinos criados en los Humedales de Paraguay durante tres periodos del año

Livestock activities, mainly those related to livestock rearing, are generally carried out in special and specific environments, susceptible to alterations, with particular and inherent characteristics of soil, vegetation and climate, not conducive to the development of the farming. Paraguay is no exception, because more than half of its territory is made up of semi-arid and wetlands, one of the most representative being the Ñeembucú wetlands and its areas of influence. In this area, the presence of certain genetic groups made up of local or naturalized bovines and bovines of transnational or exotic genetics stands out. The aim of this work was to assess protein status in cattle during the summer, fall, and winter as an indicator of adaptation to the wetlands of Ñeembucú, Paraguay. The study compared local and exotic breeds in an extensive cattle farming system, grouped by genotype into Criollo Ñeembucú; Criollo Pilcomayo; Nelore; Brangus, and Brahman. Blood levels of urea, creatinine, total protein, albumin, globulin, albumin/globulin ratio, and body condition were analyzed. The local criollo breeds had higher urea concentrations, total protein, and globulin values, most markedly in the summer. The exotic breeds had elevated creatinine levels. Nelore had the lowest marks for body condition both in the summer and winter. The analysis of main components explained over 60 % of the variation. Globulin and total protein integrated the first component in all three seasons. The most dissimilar breed pairs were Criollo Ñeembucú and Brahman, and also Criollo Pilcomayo and Nelore. The results suggest an adaptation process in the metabolic pathways of nitrogenated compounds and the potential capacity for immune response in local breeds. The results suggest that exotic breeds have mechanisms that compromise tissue protein integrity and renal function in the specific environments particular to the wetlands of Ñeembucú.

English

Bovine brucellosis is a major zoonosis caused by Brucella abortus. It is a disease with a high hygienic and economic impact that mainly affects breeders, handlers and consumers of dairy products and animal health professionals. It is therefore a real public health problem. The objective of this study was to assess seroprevalence and risk behaviour for zoonotic transmission of bovine brucellosis in Namentenga Province in Burkina Faso. To do this, a 2-degree random sampling was conducted. Thus, a blood sample was taken from 600 randomly selected cattle. The individual serological status of these 600 cattle in 60 farms was determined by two tests: Tamponed Antigen Test and indirect Enzyme Linked Immunosorbent Assay for confirmation. The frequency of risk behaviours towards this zoonotic disease was determined through two epidemiological questionnaires that identified known risk factors for the transmission of brucellosis between animals and humans. Individual seroprevalence was estimated at 6.8% CI 95% [5.4-8.2]. At least one animal was infected in 30% (18/60) of herds. Positivity in the indirect Enzyme Linked Immunosorbent Assay test was significantly associated with age, breed, livestock conditions and vaccination status in cattle. The most frequently observed risk behaviours in humans in Namentenga Province are assisted calving and abortions, handling of the runt without a glove, consumption of raw milk or unpasteurized curd milk and fresh cheese. In view of this result, Brucella abortus circulates in cattle farms in Namentenga Province. Since animal products such as milk and meat from these farms are consumed by the population, adequate measures must be taken to better protect and guide the means of prevention against this zoonosis among the inhabitants. &nbsp; Key words: Bovine brucellosis, Burkina Faso, Namentenga Province, Public health, Seroprevalence, Zoonosis.

236 Factors Influencing Clearance of Pregnancy-associated Glycoproteins in Postpartum Beef Cattle

Abstract Blood pregnancy tests can determine pregnancy status in cattle; however, pregnancy-associated glycoproteins (PAGs) have a long half-life (80 to 100 d). If tested too soon after calving, false positive readings can occur due to elevated concentrations from the previous pregnancy. This study’s objective was to determine factors that impact clearance of PAGs in postpartum beef females. Blood samples were collected from postpartum Angus and Angus-cross females (primiparous n = 46 and multiparous n = 58); once a week for up to 14 weeks after calving (range of first to last sample 1–7 to 98–105 days postpartum; dpp). Serum was tested in duplicate using the IDEXX Alertys Pregnancy Test. Data were analyzed as a repeated measure using the MIXED procedure of SAS (9.4) with parity, calf sex (sex), days postpartum, and all interactions in the model. Data were analyzed using the REG procedure in SAS. There was a significant effect of dpp (P &amp;lt; 0.01) on PAG concentrations; however, PAG concentrations were not influenced by parity (P = 0.24), sex (P = 0.49), parity by dpp (P = 0.12), parity by sex (P = 0.65), sex by dpp (P = 0.37), or parity by sex by dpp (P = 0.99). Concentrations of PAGs rapidly decreased from d 0 to 50 postpartum and then continued to gradually decrease to d 100. Days postpartum accounted for 67.53% of the variation in PAG concentrations. Prior to 50 dpp, PAG concentrations were sufficiently elevated which resulted in false positive readings (S – N ≥ 0.3). In summary, concentrations of PAGs remained elevated after calving, but the clearance of PAGs were not impacted by parity or sex of the calf. Clearance of PAGs were significantly influenced by days postpartum. Additionally, using a blood pregnancy test prior to d 50 postpartum would increase the likelihood of false positive readings due to residual PAG concentrations from the previous pregnancy.

Mobile Veterinary Clinic: an innovative approach to promote calf health

Background: Calf morbidity and mortality are of great concern for dairyman all over the world including Bangladesh. A mobile Veterinary Clinic (MVC) was established to identify the diseases associated with morbidity and mortality and provide veterinary services at farmers’ doorstep to promote calf health. Methods: A MVC was established and run from October 2011 to October 2014 to promote calf health. A total of 500 farmers from small and large holder private farms having at least two crossbred cattle were selected. A motorcycle and a mobile phone were provided to each research assistant (RA). The farmers were linked with RAs through mobile number. The ULO/VS were linked with the RA of the respective upazila for immediate treatment of the affected animal(s). The farms were under routine surveillance of the RA. In case of sickness or mortality, RA immediately contacted ULO/VS and project coordinator (PC) /principal investigator (PI) at Bangladesh Agricultural University. Necessary advice was given by ULO/VS/PC/PI to solve the problem as required. Health cards were distributed to the selected farmers for recording the health status of cattle. Diseases were confirmed by clinical and laboratory methods. Farmers were trained on better calf health management. Early disease diagnosis and treatment, routine deworming, and vaccination of cows and calves were performed regularly. Results: Before implementing MVC services, the overall mortality at herd level was 11.5% which was 8.2% and 14.7% in Muktagacha and Shahjadpur respectively. The overall morbidity was 48.5% which was higher in Shahjadpur (57.94%) than Muktagacha (33.81%). The most common causes of calf mortality were pneumonia (66.2%), unknown (17.4%), adverse drug effect (5.3%), FMD (2.6%), and sudden death (2.4%). Due to MVC at farmers’ doorstep, the overall herd level mortality reduced significantly from 11.5 to 2.9% which were 8.2 to 2.3% in Muktagacha and 14.7 to 3.6% in Shahjadpur. Conclusion: Measures to control pneumonia and FMD in calves should be undertaken. The unknown etiology of mortality should be explored. Cautions should be taken before administering drugs to calves. As MVC was successful in reducing calf mortality, we recommend replicating in other parts of the country.

The Importance of Interferon-Tau in the Diagnosis of Pregnancy.

Several decades of improving dairy cattle towards unilateral utilization of dairy cattle led to enormous progress in the field of milk yield; however, it resulted in a number of unfavorable features, such as reproductive disorders, increased calf mortality, and reduced health. Most cases of embryo loss and/or lost pregnancies occur during the first four to five weeks of gestation; accurate detection for pregnancy during this period is likely to contribute to an improvement in gestation rates. A specific protein, interferon-tau (IFNT), stimulates interferon-stimulated genes (ISGs), and their expression increases during gestation within 21 days after insemination. In bovines, the early conceptus undergoes a phase of rapid growth and elongation before implantation, the latter occurring 2–3 weeks after fertilization. IFNT acts mainly in the endometrium of the luminal epithelium. It is a new type I interferon that regulates several genes encoding uterine-derived factors. They are crucial in the processes of preparing the uterus for placenta attachment, modifying the uterine immune system, and regulating early fetal development. Because IFNT is expressed and induces ISGs in the endometrium during pregnancy recognition, it was reasoned that surrogate markers for pregnancy or IFNT might be present in the blood and provide an indicator of pregnancy status in cattle.

Theileriosis prevalence status in cattle and buffaloes in India established by systematic review and meta-analysis

The present study on theileriosis prevalence in cattle and buffaloes of India was reckoned by systematic review and meta-analysis. The studies on theileriosis prevalence reported during the period 1984-2019, were collected by using online databases, and offline literature and meta-analyses was done in R-Software. The theileriosis prevalence was 20% [95% level, CI 16-25%, PI 2-74%], obtained by using 70,688 samples. The increasing trend of theileriosis prevalence was observed in the recent period [2011-19] when compared to past years. A higher theileriosis prevalence in the Central zone [24%] than in other zones was observed. The state-wise analysis revealed a higher prevalence in Puducherry and Assam. The method-wise breakdown revealed a higher prevalence by serology for India [39%] in comparison to other methods. Host species-wise scrutiny indicated a higher prevalence in cattle [22%] than buffaloes [14%]. Theileriosis was caused by Theileria (T.) annulata [21%], T. orientalis [16%], and T. species [17%] in India. The theileriosis prevalence established by systematic review and meta-analysis in the present study forms the first report from India to the best of our knowledge. It also documents a valuable literature resource on theileriosis prevalence studies in India. This study helps in identifying the theileriosis high-risk zones and states in India. It will help the policymakers and various stakeholders in decision making and wisely using the scarce resources available in implementing preventive strategies effectively. Further, theileriosis prevention can be employed by adopting effective therapeutic measures and vector control strategies, which will augment the profits to dairy farmers in India.

Influence of Molybdenum in Drinking Water or Feed on Copper Metabolism in Cattle-A Review.

Simple SummaryThe majority of research examining the antagonist impact of molybdenum (Mo) on copper (Cu) absorption and metabolism in ruminants has been conducted by supplementing dietary Mo, alone or in combination with elevated dietary sulfur (S) concentrations, and monitoring the Cu status of the animals. However, little research has been conducted investigating the impact of Mo in water on Cu metabolism in ruminants. Based on the limited number of experiments available for this review, it appears that Mo in drinking water may have a lower antagonistic impact on Cu status in cattle when compared to Mo consumed in the diet. Therefore, this review will focus on the impact of Mo in drinking water on cattle performance and Mo and Cu metabolism.The majority of Mo research has focused on the antagonist effect of Mo, alone or in combination with elevated dietary S, on Cu absorption and metabolism in ruminants. Diets containing both >5.0 mg of Mo/kg DM and >0.33% S have been reported to reduce the Cu status in cattle and sheep. Therefore, due to the potential for inducing Cu deficiency, Mo and S concentrations in the diet should be monitored and kept within appropriate values. Elevated sulfate concentrations in drinking water can also be detrimental to livestock production, especially in ruminants. High concentrations of sulfate in water have been extensively studied in cattle because high-sulfate water induces polioencephalomalacia in ruminants. However, little research has been conducted investigating the impact of Mo in water on Cu metabolism in ruminants. Based on the limited number of published experiments, it appears that Mo in drinking water may have a lower antagonistic impact on the Cu status in cattle when compared to Mo consumed in the diet. This response may be due to a certain percentage of water bypassing the rumen when consumed by ruminants. Therefore, the objective of this review was to examine the impact of Mo in drinking water on cattle performance and Mo and Cu metabolism.