Subcooled flow boiling is a highly efficient cooling systems for thermal management systems. This study explores the intricate dynamics of subcooled flow boiling within a horizontal channel, investigating the impact of vapor generation on liquid-phase velocity using Particle Image Velocimetry (PIV) and advanced image processing techniques. Four mass flow rates ranging from 5–20 g/s with subcooled inlet conditions are investigated in a rectangular channel with single-sided heating. Three regions of interest along the heated channel are investigated for instantaneous PIV analysis. The PIV system captures detailed velocity profiles, illustrating the impact of varying mass flow rates and heat flux levels on flow behavior. Vapor masking techniques are introduced to enhance the precision of PIV data by mitigating interference from the vapor phase. Results demonstrate the influence of vapor bubbles on flow resistance, revealing non-uniform velocity distributions and turbulence near the liquid–vapor interface. The study emphasizes the critical role of inertia and buoyancy forces in shaping the velocity profiles. Moreover, the investigation sheds light on the effects of flow rates on the interfacial behaviors, hinting at a transition point between 10 and 15 g/s. In summary, this research contributes valuable insights into the nuanced dynamics of flow boiling, laying the foundation for future studies on turbulence, heat transfer, and phase-change phenomena in two-phase thermal management systems.