The present study investigated how stretching exercises (Static, Dynamic and Proprioceptive neuromuscular facilitation (PNF)) along with Caflam (diclofenac potassium) affect the flexibility of female athletes at Punjab University Level. It is a controlled experiment with (n=40) female athletes, aged 18-24 years, divided into two groups intervention (N=30) and control group (N=10) randomly. Stretching exercises were conducted through 6 weeks divided into two sessions that consisted of both static and dynamic exercises in each session. Flexibility was measured with sit and reach test in pre and post evaluation sessions. The study purpose was to identify the effect of stretching exercises on flexibility of female athletes (N=40) by using different stretching exercises techniques (static and dynamic stretching exercises) on female athletes. Paired sample t-test used and found that it showed highly significant results using “Statistical Package for Social Sciences” (SPSS) V.22 utilized. The effect size was also calculated through Cohans’D which was 1.41. The subjects were followed for 4 months to check the recurrence rate of the control & intervention groups and found that 19% having injuries of lower extremity (control group) and 11% having after post-exercise evaluation (intervention group).