School of Civil and Environment Engineering, University of Science and Technology Beijing, Beijing 100083, ChinaReceived March 13, 2013, Accepted March 30, 2013Molecularly imprinted microsphere for chloramphenicol (CAP) with high adsorption capacity and excellentselectivity is prepared by aqueous suspension polymerization, in which chloramphenicol is used as templatemolecule and ethyl acetate as porogen. The CAP-imprinted microspheres are used as high performance liquidchromatography (HPLC) stationary phase and packed into stainless steel column (150 mm × 4.6 mm i.d.) forselective separation of chloramphenicol. HPLC analysis suggests that chloramphenicol can be distinguishedfrom not only its structural analogs but also other broad-spectrum antibiotic such as erythromycin andtetracycline. In addition, the binding experiments of CAP-imprinted microspheres are carried out in ethanol/water (1:4, V:V), the results indicate that the maximum apparent static binding capacity of molecularlyimprinted microspheres is up to 66.64 mg g