Lateral ankle sprain (LAS) is the most common injury in Korean adolescent soccer players. However, intrinsic risk factors for LAS in adolescent soccer players remain unclear. Therefore, this prospective cohort study aimed to identify intrinsic risk factors for LAS in Korean adolescent soccer players. This study included 100 adolescent soccer players (age=13.21±0.95 years, height=1.62±0.09 m, weight=53.37±9.54 kg, BMI-for-age percentile=63.78±20.89%, athletic career=3.95±1.96 years). Baseline measurements were performed in the preseason, and the injury surveillance on LAS was conducted for 1 year. Independent variables included participants’ demographic and anthropometric data, self-reported questionnaire, lower extremity alignments, range of motion, static, and dynamic balance, coordination, and ankle kinematics during running. Independent t-test and Chi-square analysis were used for potential risk factors, which were imported to the binary logistic regression to identify intrinsic risk factors for LAS. Data on intrinsic risk factors identified by the logistic regression were analyzed using the receiver operating characteristic curve analysis to determine a cutoff point of each variable. In this study, age, body mass index, previous LAS history, the number of LAS history, and maximum dorsiflexion and inversion during the stance phase of running were potential risk factors for LAS. Our major findings showed that decreased dorsiflexion and increased inversion at initial contact during running significantly affected LAS occurrence in Korean adolescent soccer players. Considering our major findings, to prevent LAS in adolescent soccer players, gait training must not be overlooked and should be included in warm-up sessions.