State Of Esp Research Articles

New records of Neocrex erythrops (Sclater, 1867) (Aves, Rallidae) from Espírito Santo state, Brazil

Neocrex erythrops (Sclater, 1867), Paint-billed Crake, is a difficult bird to find and has few, scattered records spread over Brazil. The state of Espírito Santo has only one documented record of this species, which is based on a specimen collected in 1940. After 76 years since this first and only record, we present new records of N. erythrops from Espírito Santo. The new data increase the knowledge of this species in the state.

First records of Gray-hooded Gull, Chroicocephalus cirrocephalus (Vieillot, 1818) (Charadriiformes, Laridae), in the state of Espírito Santo, Brazil

Gray-hooded Gull, Chroicocephalus cirrocephalus (Vieillot, 1818), is distributed across South America and considered a resident, with individuals in their non-breeding period able to disperse over long distances. We report the first records of this species from the state of Espírito Santo, the only state on the Brazilian coast without previous records. We suggest that the geographical distribution of this species is expanding along the Brazilian coast, in accordance with what has been previously suggested for Gray-hooded Gull.

Analysis of Leaf Pigments of SJ-02 Cocoa Seedlings Under Different Irrigation Depths and Cultivation Containers

The analysis of leaf pigments helps to understand the behavior of plant species under stress conditions. Thus, the objective of this study was to evaluate the amount of leaf pigments in SJ-02 cocoa seedlings under different irrigation depths and cultivation containers. The experiment took place at the Federal Institute of Espírito Santo, Campus Itapina, in Colatina, a city located in the northwest of the State of Espírito Santo, Brazil. A total of 144 seedlings were used, distributed in a completely randomized design, in a factorial scheme (6 × 3), where the first factor consisted of six different irrigation depths: 4; 6; 8; 10; 12 and 14 mm d-1 and the second one consisted of three different cultivation containers: polypropylene bags with dimensions of 10 × 20, 15 × 25 and 13 × 35, totalling 18 treatments, with eight repetitions. Seventy-four days after sowing, total chlorophyll content, flavonoid index and anthocyanin index were evaluated. There was no interaction between the factors studied. The 7 mm d-1 irrigation depth is the most suitable because it is practical in water management. Significance was not observed between the different cultivation containers.

First record of Dipsas mikanii Schlegel, 1837 (Serpentes, Dipsadidae) from Espírito Santo state, Brazil

We present the first record of Dipsas mikanii Schlegel, 1837 from the state of Espírito Santo, southeastern Brazil. This species has a broad geographic distribution in eastern Brazil. We collected two specimens, one from Água Doce do Norte, a municipality approximately 90 km northeast from the nearest previously known locality in the state of Minas Gerais, and the second from Colatina, about 60 km east from the nearest record in Minas Gerais. We discuss and correct misidentified records of D. mikanii from the states of Rio de Janeiro and Santa Catarina.

Effect of Flooding Under the Gas Exchange of Cocoa Seedlings

Under flooding conditions, plants exhibit morphological and physiological characteristics that indicate that the plant is undergoing stress. In this sense, the objective of this work was to evaluate the gas exchange of cocoa (Theobroma cacao L.) seedlings submitted to different times of flooding. The study was carried out at the experimental farm of the Capixaba Institute for Research Technical Assistance and Rural Extension, in Linhares, North of the State of Espírito Santo, Brazil. The experimental design was completely randomized. The treatments consisted in the flooding of the seedlings on days 0 (without flooding), 1, 2, 4 and 8. The seedlings were evaluated for gas exchange by characteristics: liquid assimilation of CO2; stomatal conductance; transpiration rate; water use efficiency. The gas exchange of cocoa seedlings were influenced by the flooding period in which they were submitted with a decrease in the values liquid assimilation of CO2, stomatal conductance and transpiration rate after the fourth day of flooding, after this period the seedlings developed morphological modifications that allowed them to adapt the flooding conditions, improving the liquid assimilation of CO2.

Growth and Quality of Genotype PS-1319 Cacao Seedlings Produced Under Different Irrigation Depths

Due to the lack of information on the water demand of cocoa seedlings, empirical techniques have been used in the supply of water to the seedlings, potentializing losses in their growth and development. In this context, the present study aimed to determine the optimal irrigation depth for a good development of the genotype PS-1319 cocoa seedlings. The study took place at the Federal Institute of Espírito Santo-Campus Itapina, located in the Colatina, a city situated in the northwestern region of the state of Espírito Santo, Brazil, in an experimental greenhouse of the campus, between October 20 and December 15, 2017. The experiment was conducted in a completely randomized design (CRD) using 20 seedlings of the genotype PS-1319 cacao per treatment. The treatments consisted of daily applications of six irrigation depths, corresponding to 4, 6, 8, 10, 12 and 14 mm d-1, being evaluated their effects on the morphological parameters (leaf area; dry mass of the aerial part, dry mass of the root system and total dry mass; height of the aerial part and stem diameter) and the quality (Dickson quality index). The applied depths interfered, both in the development and the quality of the seedlings, with quadratic adjustments for the leaf area, dry mass of the aerial part and total dry mass, diameter and for the Dickson quality index. The best responses to the studied parameters were provided by the 8.33 mm d-1 depth, which is recommended as an ideal for production of genotype PS-1319 cacao seedlings.

Analysis of Climatic Risk Favorability of Grapevine Fungal Disease Occurrence for Santa Teresa, Espírito Santo State, Brazil

The State of Espírito Santo, Brazil, has micro-regions with different climatic and soil conditions, which promote grapevine cultivation vine in several municipalities. However, the grape production process is strongly threatened by foliar fungal diseases, and its control increases the cost of production significantly. In turn, the use of models of prediction of disease occurrence allows the identification of regions with climatic risk potential for grapevine. Hence, the objective of this work was to analyze the agro-climatic favorability of climatic risk for occurrence of fungal diseases of downy mildew (Plasmopara viticola) and Botrytis cinerea on the grapevine for the municipality of Santa Teresa, in the state of Espírito Santo. Predictive models of favorability of downy mildew and B. cinerea were used. The number of sprayings was determined by the calendar system and by the rainfall system, according to the length of the cycle. Therefore, a series of meteorological data from 2007 to 2016 was used. The results showed that the frequency of days with low risk of mildew was 2%, medium risk 5%, high risk 93%. For B. cinerea, these values were 32%, 68%, and 0%, with low, medium and high risk, respectively. The number of required sprayings, according to the weather conditions, was lower than the number of sprayings recommended by the calendar system. The relationship between the risk of occurrence of the evaluated diseases showed a higher agro-meteorological favorability of occurrence of mildew in relation to B. cinerea.

Determination of the Leaflet Area of Schinus terebinthifolius Raddi in Function of Linear Dimensions

The objective of this study was to select mathematical equations that best fit the estimation of the leaf area of pink pepper (Schinus terebinthifolius Raddi) from the linear leaflet dimensions. 500 leaflets with different physiological ages of a commercial plantation were collected, located in the region of Gameleira, municipality of São Mateus, North of the State of Espírito Santo, Brazil. Was measured the length (L) along the main midrib, the largest width (W) and the observed leaf area (OLA) of each sheet. The product of the multiplication between L and W of the leaflets (LW) was determined. For the modeling the measurements of 400 leaflets were used, where OLA was used in function of L, W or LW. Based on the models found, we obtained the estimated leaf area (ELA). A simple linear regression was fitted for each proposed model of OLA in function of ELA. We tested the hypotheses H0: β0 = 0 versus Ha: β0 ≠ 0 and H0: β1 = 1 versus Ha: β1 ≠ 1, using Student’s t test at 5% probability. The mean values of ELA and OLA were compared by Student’s t test 5% probability. It was determined the mean error (E), mean absolute error (MAE), root mean square error (RMSE) and Willmott d index. The best adjusted equation was chosen by linear coefficient (β0) not different from zero, angular coefficient (β1) not unlike one, non-significant values of ELA and OLA, E, EAM and RQME closer to zero and Willmott’s index d closer to one. In this way, the leaf area of leaflets of Schinus terebinthifolius Raddi can be estimated by the quadratic model equation ELA = -2.6646 + 2.2124W + 1.3953(W)2 , using only the width of the leaves as a measure.

Alometric Model for Estimation of Leaf Area of Garcinia brasiliensis Mart. Through Non-destructive Method

The objective of the present study was to test and establish mathematical models to estimate the leaf area of Garcinia brasiliensis Mart. through linear dimensions of the length, width and product of both measurements. In this way, 500 leaves of trees with age between 4 and 6 years were collected from all the cardinal points of the plant in the municipality of São Mateus, North of the State of Espírito Santo, Brazil. The length (L) along the main midrib, the maximum width (W), the product of the length with the width (LW) and the observed leaf area (OLA) were obtained for all leaves. From these measurements were adjusted linear equations of first degree, quadratic and power, in which OLA was used as dependent variable as function of L, W and LW as independent variable. For the validation, the values of L, W and LW of 100 random leaves were substituted in the equations generated in the modeling, thus obtaining the estimated leaf area (ELA). The values of the means of ELA and OLA were tested by Student’s t test 5% of probability. The mean absolute error (MAE), root mean square error (RMSE) and Willmott’s index d for all proposed models were also determined. The choice of the best model was based on the non significant values in the comparison of the means of ELA and OLA, values of MAE and RMSE closer to zero and value of the index d and coefficient of determination (R2) close to unity. The equation that best estimates leaf area of Garcinia brasiliensis Mart. in a way non-destructive is the power model represented by por ELA = 0.7470(LW)0.9842 and R2 = 0.9949.

Estimation Leaf Area by Composite Leaves of Canavalia rosea Seedlings Through Linear Dimensions From Last Leaflet

The objective of this work was to propose models of equations from measurements of the linear dimensions of the last leaflet for the estimation of the leaf area of the composite leaves of Canavalia rosea. For this purpose, 441 composite leaves of 198 seedlings were used, 45 days after sowing, produced in nursery and belonging to the Federal University of Espírito Santo, Campus São Mateus, located in the municipality of São Mateus, North of the State of Espírito Santo, Brazil. The length (L) along the main midrib and the maximum leaf width (W) of the last leaflet of each composite leaf, as well as the leaf area of all leaflets, were measured. Subsequently, it was determined the product of the multiplication of the length with the width (LW) and leaf area observed (OLA) from the sum of leaf area of leaflets in front of these measures were adjusted linear and non-linear equations of linear first degree, quadratic and power models, where, OLA was used as a dependent variable in function of L, W and LW as independent variable. Based on the models tested, we obtained equations for the estimated leaf area (ELA). The mean values of ELA and OLA were compared by Student's t test 5% probability. The mean absolute error (MAE), the root mean square error (RMSE) and the Willmott d index, were determined as criteria for validation. The best adjusted equation was chosen through the non-significant values in the comparison of the means of ELA and OLA, values of MAE and RMSE closer to zero, value of the index d near the unitary and higher values of R2. Thus, the leaf area of the composite leaf of C. rosea seedlings can be estimated by the power model represented by equation ELA = 2.2951 (LW)0.9474 quickly, easily and non-destructively.

Non-destructive Method for Estimating the Leaf Area of Pear cv. ‘Triunfo’

The present study had as objective to determine mathematical equations to estimate the leaf area of pear cv. ‘Triunfo’ using linear dimensions of the leaves. For that, 300 healthy leaves of different sizes from each quadrant of plants from the small farm of Boa Vista located in the city of Montanha, at the northern side of the State of Espírito Santo, Brazil were used. The length (L) along the main vein was measured, along with the maximum width (W) of the leaf blade and observed leaf area (OLA), in addition to the product of the length and width (LW) of each leaf. From these measurements models of linear equations of first degree, quadratic and power were adjusted and their respective R2, using OLA as dependent variable and L, W and LW as independent variable. Based on the proposed equations, the data were validated obtaining the estimated leaf area (ELA). The mean of the ELA and OLA were compared by Student t test 5% probability. The mean error (E), the mean absolute error (MAE) and the root mean squared error (RMSE) was also used as validation criterion. The best equation model was defined based on the non-significant values from the comparison of means of ELA and OLA, E, MAE and RMSE values closer to zero and highest R2. The leaf area of pear cv. ‘Triunfo’ can be estimated by the equation ELA = -0.432338 + 0.712862(LW) non-destructively and with a high degree of precision.

Estimation of Leaf Area of Jackfruit Through Non-destructive Method

The objective of this study was to determine mathematical equations that estimate the leaf area of jackfruit (Artocarpus heterophyllus) in an easy and non-destructive way based on linear dimensions. In this way, 300 leaves of different sizes and in good sanitary condition of adult plants were collected at the Federal Institute of Espírito Santo, Campus Itapina, located in Colatina, municipality north of the State of Espírito Santo, Brazil. Were measured The length (L) along the midrib and the maximum leaf width (W), observed leaf area (OLA), besides the product of the multiplication of length with width (LW), length with length (LL) and width with width (WW). The models of linear equations of first degree, quadratic and power and their respective R2 were adjusted using OLA as dependent variable in function of L, W and LW, LL and WW as independent variable. The data were validated and the estimated leaf area (ELA) was obtained. The means of ELA and OLA were compared by Student’s t test (5% probability) and were evaluated by the mean absolute error (MAE) and root mean square error (RMSE) criteria. The choice of the best model was based on non-significant comparative values of ELA and OLA, in addition to the closest values of zero of EAM and RQME. The jackfruit leaf area estimate can be determined quickly, accurately and non-destructively by the linear first-order model with LW as the independent variable by equation ELA = 1.07451 + 0.71181(LW).

Mathematical Modeling for Leaf Area Estimation From Papaya Seedlings ‘Golden THB’

The aim of this study was to select the most suitable model for leaf area estimation from papaya seedlings cv. ‘Golden THB’ using linear dimensions of leaves with unilobular and trilobular morphology. It was used leaves of 60 seedlings with 30 days after sowing produced in nursery of the Fazenda Santa Teresinha which belongs to company Caliman Agrícola S.A., in the municipality of Linhares, state of Espírito Santo, in March 2016. The measurement of the length (L) was performed along the midrib, the maximum width (W) of the leaf blade, the product of the length by the width (LW) and the observed leaf area (OLA). From these results, first degree and power linear regression models was adjusted. From the proposed regression models, the validation was performed with a leaves sample of 60 seedlings produced in June 2016, obtaining, thus, the estimated leaf area (ELA). The following criteria were used to choose the best model: the highest coefficient of determination (R2), the values do not significant of the comparison of means of OLA and ELA and values of MAE and RMSE closer to zero. The leaf area estimation from papaya seedlings cv. ‘Golden THB’ can be represented through equation ELA = -0.402619 + 0.612525(LW) for trilobular leaves and through equation ELA = 0.623355 + 0.610552(LW) for unilobular leaves.

The prevalence and phenotype in Brazilian patients with inflammatory bowel disease

BackgroundThe epidemiology of inflammatory bowel disease (IBD) varies in different countries. This study aimed to assess phenotype, medications, prevalence and incidence of IBD in the State of Espírito Santo, Brazil.MethodsPatients with IBD who utilized the Public Medication-Dispensing System of the Department of Health of Espírito Santo, between August 2012 and July 2014. Of 1484 active patients, 1048 were analyzed, which included patients of all ages.ResultsThe prevalence of IBD was 38.2 per 100,000 inhabitants, with ulcerative colitis (UC) being 24.1 per 100,000 and Crohn’s disease (CD), 14.1 per 100,000. The incidence of IBD was 7.7 per 100,000 inhabitants/year (UC – 5.3/100,000 inhabitants/year and CD – 2.4/100,000 inhabitants/year). Out of the 1048 patients analyzed, 669 had UC (63.84%), 357 had CD (34.06%), and 22 had unclassified inflammatory bowel disease (UIBD; 2.10%). There were 48/1048 (4.5%) pediatric patients (16 years of age or younger). On the UC phenotype (n = 654), we observed left-sided colitis in 247 (37.7%), pancolitis in 209 (31.9%), and proctitis in 198 patients (30.2%). Pancolitis was more frequent in pediatric patients (p = 0.007). CD showed a homogeneous distribution between ileitis (L1), colitis (L2), and ileocolitis (L3). Regarding the CD behavior (n = 352) observed the inflammatory type (B1) in 176 (50%); fistulizing (B3) in 75 (21.2%), isolated type (B3) in 29 (8.2%), and perianal fistulizing type (B3p) in 46 (13.1%). Biologic therapies were used in 154/357 (43.3%).ConclusionThe prevalence of the IBD in the state of Espírito Santo, Brazil was higher than in two other brazilian studies. There was high utilization of biologic therapies in patients with CD.

Assessment of asymptomatic Plasmodium spp. infection by detection of parasite DNA in residents of an extra-Amazonian region of Brazil

BackgroundThe hypotheses put forward to explain the malaria transmission cycle in extra-Amazonian Brazil, an area of very low malaria incidence, are based on either a zoonotic scenario involving simian malaria, or a scenario in which asymptomatic carriers play an important role.ObjectivesTo determine the incidence of asymptomatic infection by detecting Plasmodium spp. DNA and its role in residual malaria transmission in a non-Amazonian region of Brazil.MethodsUpon the report of the first malaria case in 2010 in the Atlantic Forest region of the state of Espírito Santo, inhabitants within a 2 km radius were invited to participate in a follow-up study. After providing signed informed consent forms, inhabitants filled out a questionnaire and gave blood samples for PCR, and thick and thin smears. Follow-up visits were performed every 3 months over a 21 month period, when new samples were collected and information was updated.ResultsNinety-two individuals were initially included for follow-up. At the first collection, all of them were clearly asymptomatic. One individual was positive for Plasmodium vivax, one for Plasmodium malariae and one for both P. vivax and P. malariae, corresponding to a prevalence of 3.4% (2.3% for each species). During follow-up, four new PCR-positive cases (two for each species) were recorded, corresponding to an incidence of 2.5 infections per 100 person-years or 1.25 infections per 100 person-years for each species. A mathematical transmission model was applied, using a low frequency of human carriers and the vector density in the region, and calculated based on previous studies in the same locality whose results were subjected to a linear regression. This analysis suggests that the transmission chain is unlikely to be based solely on human carriers, regardless of whether they are symptomatic or not.ConclusionThe low incidence of cases and the low frequency of asymptomatic malaria carriers investigated make it unlikely that the transmission chain in the region is based solely on human hosts, as cases are isolated one from another by hundreds of kilometers and frequently by long periods of time, reinforcing instead the hypothesis of zoonotic transmission.

Mitochondrial genome of Plasmodium vivax/simium detected in an endemic region for malaria in the Atlantic Forest of Esp\xedrito Santo state, Brazil: do mosquitoes, simians and humans harbour the same parasite?

BackgroundThe transmission of malaria in the extra-Amazonian regions of Brazil, although interrupted in the 1960s, has persisted to the present time in some areas of dense Atlantic Forest, with reports of cases characterized by particular transmission cycles and clinical presentations. Bromeliad-malaria, as it is named, is particularly frequent in the state of Espírito Santo, with Plasmodium vivax being the parasite commonly recognized as the aetiologic agent of human infections. With regard to the spatial and temporal distances between cases reported in this region, the transmission cycle does not fit the traditional malaria cycle. The existence of a zoonosis, with infected simians participating in the epidemiology, is therefore hypothesized. In the present study, transmission of bromeliad-malaria in Espírito Santo is investigated, based on the complete mitochondrial genome of DNA extracted from isolates of Plasmodium species, which had infected humans, a simian from the genus Allouata, and Anopheles mosquitoes. Plasmodium vivax/simium was identified in the samples by both nested PCR and real-time PCR. After amplification, the mitochondrial genome was completely sequenced and compared with a haplotype network which included all sequences of P. vivax/simium mitochondrial genomes sampled from humans and simians from all regions in Brazil.ResultsThe haplotype network indicates that humans and simians from the Atlantic Forest become infected by the same haplotype, but some isolates from humans are not identical to the simian isolate. In addition, the plasmodial DNA extracted from mosquitoes revealed sequences different from those obtained from simians, but similar to two isolates from humans.ConclusionsThese findings strengthen support for the hypothesis that in the Atlantic Forest, and especially in the state with the highest frequency of bromeliad-malaria in Brazil, parasites with similar molecular backgrounds are shared by humans and simians. The recognized identity between P. vivax and P. simium at the species level, the sharing of haplotypes, and the participation of the same vector in transmitting the infection to both host species indicate interspecies transference of the parasites. However, the intensity, frequency and direction of this transfer remain to be clarified.

The majestic canopy-emergent genus Dinizia (Leguminosae: Caesalpinioideae), including a new species endemic to the Brazilian state of Esp\xedrito Santo

SummarySince its description, almost 100 years ago, the genus Dinizia has been treated as monospecific, comprising the single canopy-emergent species Dinizia excelsa Ducke which grows in non-flooded Amazonian forests of Guyana, Suriname and seven states of northern and central-western Brazil. Dinizia jueirana-facao G. P. Lewis & G. S. Siqueira, which grows in a restricted area of semi-deciduous Atlantic rain forest in Espírito Santo state, Brazil, is described as a new species in the genus. The new species is also a canopy-emergent of impressive stature. We provide descriptions for both species, a key to species identification, a distribution map and the new species is illustrated. Fossil leaves, inflorescences and fruit provide evidence for a Dinizia-like ancestor occurring in south-eastern North America during the Eocene. In contrast to D. excelsa where pollen is dispersed in tetrads, the pollen of D. jueirana-facao is shed in monads. D. jueirana-facao is considered critically endangered following IUCN conservation criteria, whereas D. excelsa is assessed to be of least concern. A lectotype is designated for D. excelsa.

Drug use prevention projects in schools in Vit\xf3ria, Brazil: quality analysis and improvement proposals

Adolescents living in vulnerable regions are more exposed to risk factors for drug use. The prevention of such use in school is a public policy that needs evaluation. Based on technical criteria and derived from a mixed research, this article analyses the quality of school-based prevention of drug use in Vitória, state of Espírito Santo, Brazil, and proposes improvements. A checklist of quality elements was completed with data from 16 projects proposed by 99 teachers from public schools. In 10 projects (62.5%), the approximate quality index was above 0.50. The majority of projects fulfilled the requirement of theoretical foundation (81.25%) and some of the methodological (93.75%), design (75%) and implementation (62%) requirements. Other requirements were absent: the majority were not designed by the whole school community (87.5%), and the participation of the family (62.5%) or the students as mediators (62.5%) was not considered. In general, contents of life skills (87.5%), positive relationships and alternative activities to drug use (56.25%) were not included. Activities for reinforcing the content were not described in any of the projects, and evaluation activities were described in only a few (31.25%). Many projects did not describe the inclusion of the project in the school curriculum (62.5%). Although, considering all items of effectiveness, regardless of their weight, more than half of the projects had an above average quality. The present items provide quality to the projects, whereas absent items indicate shortcomings to be improved using some of the measures described in this study.