States In Peninsular Malaysia Research Articles

Climate change impact under CanESM2 on future rainfall in the state of Kelantan using Artificial Neural Network

Kelantan is a state in Peninsular Malaysia that is highly vulnerable to extreme events such as drought and floods which are becoming worse because of climate change due to global warming that is caused by human activities. This study aims to evaluate the potential impacts of climate change on the future of rainfall in Kelantan using Artificial Neural Network. CanESM2 under three Representative Concentration Pathways (RCPs), namely RCPs 2.6, 4.5, and 8.5 for 2011-2100 are incorporated with the ANN model and are used to compare the baseline period (1972 to 2018). In general, the simulated rainfall that downscaled by using the ANN model approximates the observed rainfall (during the calibration and validation periods) reasonably well. The study also shows that the ANN model anticipates a major increase in annual rainfall in the 2080s for the RCP 8.5 scenario.

Investigating the structural relationship of mass media on attitude, social influence, product safety and its impact on purchase behaviour of herbal product in Malaysia

Mass media is a crucial advertising platform and serves as an awareness platform for herbal product businesses. The rising popularity of herbal product consumption is due to the changing of lifestyle and health problems. With the increasing demand for herbal products globally, studies related to the herbal products purchase behaviour are considered very limited. Hence, the current study is written to investigate the structural relationship of mass media on attitude, social influence, product safety and its impact on purchase behaviour of Herbal products in Malaysia. By applying the Attitude-Behaviour Context Theory, this study used several variables, namely mass media toward attitude, social influence, and product safety and purchasing behaviour of herbal products. This study was designed using a quantitative approach through a survey questionnaire. A total of 467 respondents from seven states in Peninsular Malaysia have participated in this study and collected using the intercept survey. The data were analysed using structural equation modelling with a partial least square approach (SEM-PLS) and assisting SmartPLS 3.3.3. The results showed that mass media has a significant positive effect on attitude, social influence and product safety. Also, attitude, social influence and product safety have a significant positive impact on the purchase behaviour of herbal products. In conclusion, this study identified that mass media influence on attitudes is the strongest predictor, followed by the effects of the mass media on social influence. These findings indicated that the mass media play an essential role in influencing attitude, social influence, and product safety. Followed by attitude, social influence, and product safety that significantly impact the purchase behaviour of herbal products. The finding of this study provides insightful information to the entrepreneurs regarding consumers purchase behaviour.

Malaysian Public’s Concern About the Environment During the Covid-19 Pandemic: A Study of a Selected State in Peninsular Malaysia

This article examines the level of concern of the Malaysian community towards the environment in a selected state of Peninsular Malaysia during the Covid-19 pandemic. The findings of the study presented are based on a quantitative study involving a total of 211 respondents. This is part of the findings of a real study involving the Malaysian society in general across the state. Overall, this case study found that the respondents' level of concern for the environment during the Covid-19 pandemic was at a high level. However, the level of green consumption practices in society was still seen at a moderate level. There was no significant difference in terms of their level of concern for the environment during the Covid-19 pandemic either among respondents living in urban or rural areas. Similar findings were shown for single and married respondents. In short, the findings of the study show a very positive sign among some parts of Malaysian society towards realising the efforts to preserve the environment by various parties in Malaysia. Therefore, this study argues that the efforts to educate the community that have been done by various parties should be seen as a profitable investment. In fact, it is necessary to secure continuous support from various parties in the community towards the preservation of a sustainable environment, especially in terms of increasing the level of green consumption practices among the community.

ADOPTION OF SOCIAL MEDIA MARKETING AMONG AGROPRENEURS IN PENINSULAR MALAYSIA

Social media has become one of the main platforms for businesses around the world to markettheir products or services. However, some entrepreneurs still feel doubtful whether social mediamarketing is effective enough compared to the traditional marketing on seeking attention fromtheir targeted audience based on the different generation. Besides, people still think that socialmedia is used as the medium for people around the world to socialize with everyone instead ofmarketing their product or services, especially agro-based products. Entrepreneurs also did not usesocial media marketing because they do not have the sufficient knowledge on how to fully utilizethe social media to market their product. This will hinder them from using social media marketingin their business because they might need more time and assistance to learn how to employ socialmedia marketing. Thus, the main objective of this study is to investigate the adoption of socialmedia marketing among agropreneurs in Peninsular Malaysia. Primary data was collected usingstructured questionnaire via online survey. The questionnaire have been distributed through socialmedia platforms and e-mailed to the targeted respondents using purposive sampling method. Atotal of 113 respondents from 12 states in Peninsular Malaysia was successfully participated inthis study. Data collected was analyzed using descriptive analysis, chi-square analysis and factoranalysis. The results of this study indicated that the adoption level of social media marketingamong agropreneurs in Peninsular Malaysia is on the medium high level and the highest socialmedia platform adopted is Facebook. Besides that, the results of chi-square analysis showed thatrespondent’s demographic and business profile including gender, age, education level and typesof agro-based business operated has an association with the adoption of social media marketingamong agropreneurs. The results of factor analysis discovered four factors that influencingadoption of social media marketing among agropreneurs namely; perceived usefulness, socialinfluence, perceived ease of use and facilitating conditions. Hence, entrepreneurs that alreadyadopted social media marketing should cooperate and create an association of online agropreneursto encourage other entrepreneurs so that they start adopting social media marketing.

Factors influencing pregnancy planning of multi-ethnic Asian women with diabetes: A qualitative study.

IntroductionPregnancy planning varies among women with diabetes. Observing that the literature examining the factors affecting diabetic women’s pregnancy intentions in multi-ethnic Asian populations is limited, we sought to explore these factors to give a better perspective on these women’s pregnancy planning.MethodsThis qualitative study used individual in-depth interviews to capture the views and experiences of non-pregnant diabetic women of reproductive age in four public health clinics in a southwestern state of peninsular Malaysia from May 2016 to February 2017. The participants were purposively sampled according to ethnicity and were interviewed using a semi-structured topic guide. Interviews were audio-recorded, and transcripts were analysed using thematic analysis.ResultsFrom the 33 interviews that were analysed, four important factors influencing participants’ decisions regarding pregnancy planning were identified. Participants’ perception of poor pregnancy outcomes due to advanced age and medical condition was found to have an impact. However, despite these fears and negative relationships with doctors, personal, family and cultural influences supported by religious ‘up to God’ beliefs took centre stage in the pregnancy intention of some participants. Participants demonstrated a variety of understandings of pregnancy planning. They outlined some activities for pregnancy preparation, although many also reported limited engagement with pre-pregnancy care.ConclusionsThis study emphasised the known dilemma experienced by diabetic women considering their desire for an ideal family structure against their perceived pregnancy risks, heterogeneous religious beliefs and the impact of cultural demands on pregnancy intention. This study urges healthcare providers to increase their engagement with the women in pregnancy planning in a more personalised approach.

Health promotion and active aging among seniors in Malaysia

PurposeThis paper explores the relationship between health promotion and active aging among seniors in Malaysia. The specific objectives were to (1) identify the contributing factors for seniors undergoing full medical check-up and (2) to explore the association between selected active aging factors and health promotion behavior and beliefs.Design/methodology/approachThe study used data from 662 seniors from three different states in Peninsular Malaysia. Logistic regression was used to identify significant determinants of full medical check-up, and chi-square statistics were used to explore the association of active aging and selected health promotion behavior and beliefs. Healthy aging was characterized by being employed or traveling outdoors for leisure.FindingsHousehold income was found to be a significant barrier to seniors undergoing a full medical check-up. Overall, active seniors were more likely to have positive self-rated health, positive health responsibility and health promotion beliefs but were less likely to undergo a full medical check-up.Practical implicationsGiven that cost of a medical check-up could be a barrier for seniors, authorities could consider subsidizing medical check-ups to promote early detection of disease. There is also a need for continuous effort to educate seniors on health risk factors and the importance of taking fuller responsibility for their own health.Originality/valueThis study examined the relationship between active aging and health promotion together, as both components are essential in enhancing the mental and physical well-being of seniors.

Reliability and Validity of the Malay Version of Edinburgh Postpartum Depression Scale (EPDS) When Administered to Postpartum Mothers at Two Points in Time

Introduction: Edinburgh Postpartum Depression Scale (EPDS) is a tool used to assess the risk of postpartum depression (PPD). In this study we determined the reliability and validity of the Malay version of EPDS when administered at two different time points in the postpartum period. Materials and Methods: This cross-sectional study design was carried out between May and September 2017 at three government primary healthcare clinics located in Batang Padang district, a suburban area of Perak state in Peninsular Malaysia. We recruited a total of 89 women; 41 women were in the early postpartum period (1-30 days) and 48 women were in the late postpartum period (31-120 days). Cronbach's alpha coefficient, inter-item correlation, and corrected item-total correlation were used to assess the internal consistency. The concurrent validity was assessed using Spearman’s correlation. The data were analyzed using SPSS version 20 and R 3.4.2. Results: The Cronbach’s alpha for the first and second group was 0.78 and 0.62, respectively, which indicated satisfactory reliability. At both time periods, removing Item 2 from the scale resulted in a significant increase in Cronbach’s alpha (to 0.847 and 0.709, respectively). As expected, the EPDS scores correlated moderately with the BDI-II scores (1−30 days: Spearman's rho = 0.65, p < 0.01; 31−120 days: Spearman's rho = 0.73, p < 0.01). Conclusion: The Malay version of the EPDS is a reliable screening instrument for detecting postpartum depression. It showed reasonability and feasibility and can be used in postpartum clinical settings or for assessing intervention effects in research studies. Furthermore, as our results indicated, removing Item 2 from the Malay version would increase the internal consistency of the EPDS.

Cow- and herd-level factors associated with lameness in dairy farms in Peninsular Malaysia

Lameness is a major welfare issue in dairy cows. This study was aimed at investigating the cow- and herd –level factors associated with lameness in dairy farms from four states in Peninsular Malaysia. The study population was 1001 lactating cows from 28 dairy farms located in Selangor (n = 9), Perak (n = 8), Negeri Sembilan (n = 6) and Johor (n = 5). Lameness was assessed by locomotion scoring. Individual cow characteristics such as breeds, parity, body condition score (BCS), hock condition, leg hygiene, presence of claw lesion and claw overgrowth were recorded. Data on herd characteristics, management practices and housing design were collected by on-farm inspection and farmers’ interview. Mixed-eff ;ects logistic regressions were used to model the data and to assess the factors associated with lameness. Cow-level lameness prevalence was 34.2 % (95 % CI 22.2–50.0 %), with all the farms having at least one case of lameness. Claw lesions were recorded in 470 cows (46.9 %; CI 33.3–63.3 %) of which 296 (62.9 %) of them were lame. Of these, 78.9 % of the lesions were present on the rear feet and 25.5% of the cows had more than one foot affected. The proportion of cows having non-infectious and infectious claw lesions were 81.9 % and 18.1 %, and the predominant claw lesions were sole ulcers (24.9 %), white line disease (19.6 %), sole haemorrhage (10.2%), swelling of coronet (9.6 %), toe ulcers (8.4 %), and digital dermatitis (5.6%). Cows at third or more parities had higher odds of lameness (OR = 2.2; 95 % CI 1.2–4.1) compared to primiparous cows. Low BCS (< 2.5) increased the odds of lameness (OR = 4.8; 95 % CI 2.9–7.9) relative to cows with moderate BCS, and cows with hair loss around the hock (OR = 1.4; 95 % CI 1.1–1.9) relative to those with normal hock condition. Greater odds of being lame was observed in cows having claw lesion (OR = 15.2; 95 % CI 10.4–19.2) and those with overgrown claw (OR = 3.3; 95 % CI 2.4–4.5). There was increased odds of lameness in farms with high stocking density (OR = 1.8; 95 % CI 1.1−3.1), concrete floored walkways (OR = 1.9; 95 % CI 1.0–3.6), dirty floors (OR = 2.3; 95 % CI 1.9–3.7), and practicing preventive claw trimming (OR = 2.3; 95 % CI 1.9–4.6). Based on the high lameness prevalence, these findings could assist dairy farmers to make informed decisions on areas to implement on-farm changes to reduce lameness in the studied herds.

VIOLENCE-RELATED BEHAVIORS AMONG SCHOOL GOING ADOLESCENTS IN PENINSULAR MALAYSIA

Violence among adolescents is a significant public health issue. The focus was on school-going adolescents because of the life-long negative impact at an individual level both for the perpetrator and victim. Besides, it increases the costs to provide health, welfare, and criminal justice as well as decreases general productivity at the community level. The study aims to determine the prevalence of violence-related behavior, characteristics of both victims and perpetrators, in addition to inter and intrapersonal factors associated with violence-related behaviors. A study was carried out in 2013, which involved all upper secondary school students (aged 16 to 17 years). It was a part of the Malaysian Adolescent Health Risk Behavior (MyAHRB) study, which was conducted in 11 states in Peninsular Malaysia. Standardized, validated questionnaires were used for data collection. The prevalence of violence-related behaviors was 22.4%, and this was higher among male students as compared to females (29.1% vs. 16.3%). Multivariable analysis revealed that the odds of violence-related behaviour increased among males, Malay race, drug use, smoking, had exposure to sex, which sometimes and always felt lonely and had attempted suicide. Agreeable to ensure that their lifestyles do not transgress religious limit and belief was significantly protective. This study provides evidence for a targeted approach to combat violence-related behaviors among adolescents.

Molecular DNA-based spatial mapping technique predicting diversity and distribution of otters (Lutrinae) in Peninsular Malaysia using non-invasive fecal samples

This study investigated the current distribution of otters (Lutrinae) in all states of Peninsular Malaysia. Four species of otters have been recorded in Peninsular Malaysia in the past based on field survey methodology, namely the Asian small-clawed otter (Aonyx cinereus), smooth-coated otter (Lutrogale perspicillata), hairy-nosed otter (Lutra sumatrana), and Eurasian otter (Lutra lutra). This study aims to determine the current number of otter species and their distribution in Peninsular Malaysia through molecular approaches using D-loop and Cyt b loci of mitochondrial DNA sequences. Our findings indicate that 337 samples were identified as three species of otter that are currently distributed in Peninsular Malaysia: A. cinereus (112 samples), L. perspicillata (218 samples), and L. sumatrana (7 samples). All three species can be found in Perak, Kelantan, Pahang, Selangor, and Johor while other states only contained two species (A. cinereus and L. perspicillata). Seven sympatric locations were recorded between two or three species: Tumpat and Kg. Padang Salim (Kelantan), Penarik and Sg. Kemaman (Terengganu), Taman Negara (Pahang), Tg. Piai (Johor), and Kuala Gula (Perak). The discovery of L. sumatrana in this study has confirmed that this species is still present in Peninsular Malaysia. However, L. lutra was not observed in this study. Our findings successfully highlight the diversity and distribution of Peninsular Malaysian otters that suggest the importance of continuous assessment and population status of otters to ensure their ongoing survival in Peninsular Malaysia.

Parents' and caregivers' satisfaction with community‐based rehabilitation (CBR) services for children with disability in east coast states in Peninsular Malaysia

The Community-Based Rehabilitation (CBR) services under the Malaysian Ministry of Women, Family and Community Development have provided two types of services for disabled children: centre-based and home-based care since 1984. A cross-sectional study was conducted among parents and caregivers with children receiving treatment at CBRs on the east coast of Peninsular Malaysia, to determine the level of satisfaction with the services provided. Respondents were recruited via multi-staged sampling, and simple randomisation at CBR level. Respondents self-administered the Physical Therapy Satisfaction Questionnaire (PTSQ) and provided sociodemographic data. A total of 297 respondents were recruited and all the questionnaires were returned complete, making the response rate 100%. Total satisfaction scores and factors influencing respondents' satisfaction for both groups were analysed. The mean total satisfaction score for centre-based and home-based were 84.69 (SD=10.01) and 75.30 (SD=12.23; t=7.160, p≤.001) respectively. Ninety-eight per cent of centre-based and 89% of home-based respondents were satisfied with the current services. There were significantly mean differences in the satisfaction level of respondents of different states respectively (Kelantan mean 84.92 10.83, Terengganu mean 77.49 11.16 and Pahang mean 77.47 12.93, p≤.001). Factors associated with satisfaction were education (p=.002), occupation (p=<0.001), monthly income (p=.001) and source of income (p=.001). In conclusion, majority of parents and caregivers were satisfied with current services provided at CBR and satisfaction with services was dependent on CBR centre location and education and financial earning capacity.

Natural Plasmodium infection in wild macaques of three states in peninsular Malaysia

Zoonotic cases of Plasmodium knowlesi account for most malaria cases in Malaysia, and humans infected with P. cynomolgi, another parasite of macaques have recently been reported in Sarawak. To date the epidemiology of malaria in its natural Macaca reservoir hosts remains little investigated. In this study we surveyed the prevalence of simian malaria in wild macaques of three states in Peninsular Malaysia, namely Pahang, Perak and Johor using blood samples from 103 wild macaques (collected by the Department of Wildlife and National Parks Peninsular Malaysia) subjected to microscopic examination and nested PCR targeting the Plasmodium small subunit ribosomal RNA gene. As expected, PCR analysis yielded significantly higher prevalence (64/103) as compared to microscopic examination (27/103). No relationship between the age and/or sex of the macaques with the parasitaemia and the Plasmodium species infecting the macaques could be identified. Wild macaques in Pahang had the highest prevalence of Plasmodium parasites (89.7%), followed by those of Perak (69.2%) and Johor (28.9%). Plasmodium inui and P. cynomolgi were the two most prevalent species infecting the macaques from all three states. Half of the macaques (33/64) harboured two or more Plasmodium species. These data provide a baseline survey, which should be extended by further longitudinal investigations that should be associated with studies on the bionomics of the anopheline vectors. This information will allow an accurate evaluation of the risk of zoonotic transmission to humans, and to elaborate effective strategies to control simian malaria.

The Use of Reflection-for-Action in Planning English Language Lesson at Primary School

Teaching English language is a challenging task for primary school teachers. Teachers need to plan their lesson to match all levels of students in one class as there is no streaming classes allowed. This study explored how reflection-for-action to be used by the English language teachers at primary schools in Northern state of Peninsular Malaysia in planning their teaching starting from choosing lesson objectives, planning the activities and preparing the materials. Hence, this study also gathered another relevance data on principles, benefits, changes and stages of reflection which led the teachers to use reflection-before-action in teaching English language. There were six participants involved in this study, three of them were male. The participants were experienced teachers who teach English language at primary schools with more than eighteen years teaching experience. This study used semi-structured interviews to collect the data. The researchers used thematic analysis to analyze the data. The findings revealed that reflection-for-action was very helpful to help the teachers to plan a meaningful lesson, to improve their teaching and to maximize their students' learning and knowledge of the target language. After applying reflection-for-action, the English language teachers managed to plan specific teaching activities to deliver specific skills in English language classroom to suit their teaching and learning contexts. Therefore, the four main themes found in this study contribute a recommendation for the teachers to learn and to use reflection-for-action dynamically to plan a meaningful lesson for multiple intelligence students in the classroom. Hence, the findings also found that teachers need to apply theory in practice to plan and reason their lesson plan arrangements to fulfil the nature of teaching and learning process which needs them to attain the skills, objectives, knowledge, experiences, subject to be taught and the materials to be learned in the current teaching and learning context.

Introduction of Exercise for Medicine (Exfome) Programme Among Patients In Primary Health Clinics In Malaysia

INTRODUCTION: Diseases due to unhealthy lifestyles such as heart diseases and diabetes are increasing in Malaysia. Heart diseases are the leading cause of deaths of patients in government hospitals, i.e. 16.1% of total deaths in 2009. A customised exercise regime called Exercise for Medicine (EXFOME) was introduced to improve and prevent chronic diseases such as diabetes, heart disease, obesity and hypertension.&#x0D; &#x0D; MATERIAL &amp;amp; METHODS: A total of 126 participants from government health clinics in three states of Peninsular Malaysia were selected to participate in the EXFOME. Each participant was evaluated prior to the program using an assessment protocol. Fitness assessment and evaluation with body composition measurement were taken. Exercise therapist prescribed a personalized exercise program according to conditions.&#x0D; &#x0D; RESULTS: Effect of exercise was measured in terms of improvement in hypertension and diabetes, body weight, body fat, lipid profile and physical fitness at three and six months. &#x0D; &#x0D; CONCLUSION: EXFOME is beneficial to improve the status of hypertension, diabetes, body weight and lipid profile when it is carried out for longer period.

Genetic diversity and differentiation in the blackflies Simulium cheongi, Simulium jeffreyi and Simulium vanluni (Diptera: Simuliidae) in Peninsular Malaysia

The population genetic structures of S. vanluni, S. cheongi and S. jeffreyi were determined from mitochondria-encoded sequences of cytochrome c oxidase subunits I (COI) across different states in Peninsular Malaysia. High levels of genetic diversity and genetic differentiation were observed among three species. All three species revealed an intermediate level of gene flow among the populations. Negative values of Fu's Fs and low values of Raggedness index supported the hypothesis of population expansion in S. vanluni, S. cheongi and S. jeffreyi.

Updates on malaria incidence and profile in Malaysia from 2013 to 2017

BackgroundTo date, most of the recent publications on malaria in Malaysia were conducted in Sabah, East Malaysia focusing on the emergence of Plasmodium knowlesi. This analysis aims to describe the incidence, mortality and case fatality rate of malaria caused by all Plasmodium species between Peninsular Malaysia and East Malaysia (Sabah and Sarawak) over a 5-year period (2013–2017).MethodsThis is a secondary data review of all diagnosed and reported malaria confirmed cases notified to the Ministry of Health, Malaysia between January 2013 and December 2017.ResultsFrom 2013 to 2017, a total of 16,500 malaria cases were notified in Malaysia. The cases were mainly contributed from Sabah (7150; 43.3%) and Sarawak (5684; 34.4%). Majority of the patients were male (13,552; 82.1%). The most common age group in Peninsular Malaysia was 20 to 29 years (1286; 35.1%), while Sabah and Sarawak reported highest number of malaria cases in age group of 30 to 39 years (2776; 21.6%). The top two races with malaria in Sabah and Sarawak were Bumiputera Sabah (5613; 43.7%) and Bumiputera Sarawak (4512; 35.1%), whereas other ethnic group (1232; 33.6%) and Malays (1025; 28.0%) were the two most common races in Peninsular Malaysia. Plasmodium knowlesi was the commonest species in Sabah and Sarawak (9902; 77.1%), while there were more Plasmodium vivax cases (1548; 42.2%) in Peninsular Malaysia. The overall average incidence rate, mortality rate and case fatality rates for malaria from 2013 to 2017 in Malaysia were 0.106/1000, 0.030/100,000 and 0.27%, respectively. Sarawak reported the highest average incidence rate of 0.420/1000 population followed by Sabah (0.383/1000). Other states in Peninsular Malaysia reported below the national average incidence rate with less than 0.100/1000.ConclusionsThere were different trends and characteristics of notified malaria cases in Peninsular Malaysia and Sabah and Sarawak. They provide useful information to modify current prevention and control measures so that they are customised to the peculiarities of disease patterns in the two regions in order to successfully achieve the pre-elimination of human-only species in the near future.

Microplastics Occurrence in Waters off the Northwest Coast of Peninsular Malaysia: A Spatial Difference

Microplastics pollution has been receiving extensive attention globally with its presence felt in both remote and pristine coastal marine environments. Defined as plastics with diameters of 5mm or less and originating from primary and secondary sources, its impact is still not fully understood. Our investigation was conducted along two northern states in Peninsular Malaysia, i.e. Pulau Pinang and Kedah, to quantify microplastics occurrence in the surface waters. Seawater samples were collected from different sampling stations using a 355µm manta net. The plastics was removed from the water samples by density separation, treated with hydrogen peroxide and were identified using a stereo microscope. Microplastics occurred at all sampling stations with numbers varying from 8 to 73 particles/L. Samples were dominated by fragment and filament shapes. Sizes (2 to 3mm) and white colouration were found to be dominant. Future research is suggested to the identification of polymer type and a better understanding of its impact on marine communities.

Etiologies of tropical acute febrile illness in West Pahang, Malaysia: A prospective observational study

Objective: To determine the etiologies of tropical acute febrile illness (TAFI) in West Pahang, Malaysia and to investigate morbidity and mortality factors in relation to TAFI. Methods: A multicenter prospective cohort study was conducted between January and June 2016 in six district hospitals throughout the western part of Pahang State in Peninsular Malaysia. A total of 336 patients answered a standardized questionnaire and blood samples were collected for laboratory confirmation of infectious etiology. Descriptive analysis and logistic regression were performed to identify factors associated with TAFI. Results: A total of 336 patients were included. The patients were mainly Malays (70.2%), males (61.3%), aged (44.6±17.4) years, with more than half (58.9%) presenting with gastrointestinal symptoms. The majority were diagnosed with dengue (35.7%) while malaria (4.5%) was the least frequent. The in-hospital mortality due to TAFI was 9.2%. Patients with meliodosis had five times higher mortality [Adjusted OR: 5.002, 95% CI: (1.233, 20.286)]. Patients with comorbidities such as cardiovascular symptoms (P Conclusions: The etiology of TAFI in the western Pahang includes dengue, leptospirosis, malaria and melioidosis, which carry the highest risk of in-hospital mortality. The presence of cardiovascular symptoms may be used to assess the disease severity in TAFI, but more studies are needed in the future.

Multiplex real-time PCR revealed very high prevalence of soil-transmitted helminth infections among aborigines in Peninsular Malaysia

Objective: To determine the true prevalence of soil-transmitted helminth infections in the Malaysian aborigines using real-time PCR.Methods: A total of 122 aborigines from seven tribes were recruited from settlements and nearby hospitals which served the communities, located in four states in Peninsular Malaysia. The stool samples were examined for the presence of soil-transmitted helminth using real-time PCR and microscopy. The latter included the direct wet mount and formalin-ether concentration technique (FECT). The infection load in FECT-positive samples was determined by the Kato-Katz method. Rotorgene real-time analyzer detected five helminth species using two sets of assays.Results: The real-time PCR detected soil-transmitted helminth in 98.4% samples (n=122), which were 1.56 times higher than by microscopy. Ascaris lumbricoides and Trichuris trichiura were detected in more than 90% of the samples, while hookworm was detected in 46.7% (Necator americanus) and 13.9% (Ancylostoma sp.) of the samples. Comparison with previous reports on the Malaysian aborigines showed that the real-time PCR markedly improved the detection of Ascaris lumbricoides, hookworm and Strongyloides stercoralis. The real-time PCR detected poly-helminths in 92.6% of the samples compared to 28.7% by microscopy. In addition, 27 samples (22.1%) showed amplification of Strongyloides stercoralis DNA.Conclusions: The real-time PCR showed very high prevalence rates of soil-transmitted helminth infections in the aborigines and is the recommended method for epidemiological investigation of soil-transmitted helminth infections in this population.

Molecular Prevalence and Epidemiology of Trypanosoma evansi Among Cattle in Peninsular Malaysia.

Animal trypanosomiasis (Surra) caused by Trypanosoma evansi (T. evansi) is known to be one of the important haemoprotozoan parasites that causes great economical loss on animal production due to mortality and loss of condition. A cross-sectional study was designed to evaluate the prevalence and risk factors associated with T. evansi infection among cattle in Peninsular Malaysia. Polymerase chain reaction (PCR) was employed on 1045 blood samples collected from 43 farms. A well-structured questionnaire was used to collect data on risk factors associated with T. evansi prevalence. The RoTat 1.2 set of primers was used to amplify products of 205 base pair. The overall prevalence was found to be 17.9% (187/1045; 95% CI = 15.66-20.31). Trypanosoma evansi was detected among cattle in all the States of Peninsular Malaysia. Breeds of cattle and closeness to waste area, where the risk factors significantly (p < 0.05) associated with the PCR positivity of T. evansi among cattle in Peninsular Malaysia. This appears to be the first time a comprehensive survey on the prevalence and risk factors of T. evansi infection in cattle using molecular tools is been carried out in the entire states of Peninsular Malaysia. The findings from this study will provide baseline information on the molecular prevalence of the haemoflagellate and its associated risk factors among cattle for an improved beef and dairy production in Peninsular Malaysia.