Study regionThe Hongze Lake Basin, China. Study focusA drought index is an essential tool for monitoring and prediction, and the construction of a comprehensive drought index is at the cutting edge of current drought research. Considering the regulation effect of lakes, a new drought index aimed at large lake catchment areas, namely the Multivariate Standardized Drought Index for Lakes Basin (MSDILB), can comprehensively characterise meteorological and hydrological drought. Here, the Standardised Precipitation Evapotranspiration Index (SPEI), Standardised Runoff Index (SRI), and MSDILB drought indices were used to evaluate the evolution of drought in the study area. Moreover, the response and conversion relationships between the basin’s hydro-meteorological elements and the regulation of the lake were explored. New hydrological insights for the RegionThe results indicate that: 1) in a dry year, the spatial-temporal heterogeneous runoff in the basin is redistributed by the storage and regulation of large lakes, thereby reducing the frequency of drought events; 2) the MSDILB drought index merges the characteristics of hydrological drought and meteorological drought indices and can monitor the evolution of drought events in lake basins; 3) the MSDILB drought index can sensitively capture the effects of lake hydrological regime change on drought events in the basin. Thus, it has the advantage of comprehensively characterising the drought conditions in large lake basins.