The mechanism related to the practice of oral carbohydrate (CHO) supplementation prior to intense exercise of a short duration (<30 min) for athletic horses remains unclear. Several studies in human athletes showed that the central nervous system played an important role in the enhancement of athletic performance due to CHO contact with undefined receptors in the oropharyngeal mucosa. This study aims to investigate the influence of CHO mouth rinse on the exercise performance of horses. In this double blind, randomised, placebo-controlled study, seven Mangalarga Marchador horses underwent a standardised exercise test (SET) in a field. The following protocols involving a single mouthwash (1 litre) had been used: a mouth rinse of 6.4% maltodextrin-water solution (CHO test), and a mouth rinse of only water solution (placebo test). The mean plasma lactate concentration immediately after SET (lactate peak) was higher in the CHO test (24.33±3.72 mmol/l) than in the placebo test (18.19±4.01 mmol/l), (P<0.001). No difference was observed in the mean time-to-exhaustion, mean and maximum heart rate, plasma glucose concentration and serum creatinine-kinase activity. Several studies described that there were improvements in the time-trial performance of cyclists and runners after a mouth rinse of CHO solution, whereas some did not. Carbohydrate mouth rinse had no influence on exercise performance in this study. Further investigations are required, as the significance of an increased plasma lactate concentration due to a CHO mouth rinse has yet to be elucidated.