Standard Operating Procedures Research Articles

Analysis of the Implementation of Standard Operating Procedures in Archives Management at PT Pelabuhan Indonesia (Persero)

This research was motivated by the application of Standard Operating Procedures (SOP) for archive management which was not yet effective when the researcher carried out practical field work activities carried out by employees. The aim of the research is to find out how the records management system works, how the records management SOP is implemented and how the effects of the records management SOP are implemented. This research uses a qualitative approach using observation, interviews and documentation methods. The data used in this research is primary data, namely data collected directly by researchers. Phenomenally, based on field observations, we see that space infrastructure is increasingly lacking in space, this is due to the increasing volume of archives. Meanwhile, during observations of archive sorting activities, several other practitioners felt confused about categorizing letters/documents that would be archived by different work units. With the Standard Operating Procedure indicators for archives, the application of SOPs to archive management and the influence of the application of SOPs to archive management. The results of this research conclude that the records management system is running well and in accordance with the applicable SOPs as its implementation is running well and even has a good impact on employee performance. Not only that, in terms of perceived obstacles, we also have good solutions to continually improve employee performance. This is one of the best steps in implementing records management in companies.

Drug supply management at first-level public health facilities: Case of Pyay District, Myanmar.

First-level public health facilities (PHFs) serve as primary providers of essential medicines, necessitating critical attention to drug availability and quality assurance. This study aimed to examine the status of functional areas within the drug supply chain management framework and assess the overall capability maturity at first-level PHFs. The cross-sectional study was conducted among 183 drug store sites from six townships of Pyay District. Only situational analysis was exercised to determine the existing situations. The overall capability maturity was determined according to the definitions of levels of the Capability Maturity Module Tool. 58.47% lacked formal drug supply management training, with 23.5% not undergoing performance reviews. Drug forecasting predominantly relied on a pen-paper system (91.6%) and factors like patient load (87.39%), drug consumption (85.71%), and disease prevalence (64.71%). Store site analysis revealed that 65.03% exhibited marginal capability, lacking standardized drugstores and employing unstandardized procedures. Storage practices varied, with 48.69% storing drugs conveniently and others categorizing them by drug type (32.79%) or using the first-expired-first-out system (40.98%). Approximately 42.69% reported having expired drugs. Concerning transportation costs, 37.16% incurred expenses exceeding 20,000 Kyats per time, with management staff often covering the costs. Waste management methods included burial pits (49.18%), incineration (62.84%), and sharp pits (55.19%). A majority (78.14%) used safety boxes, and 57.38% implemented a color-coded system for waste bins. The logistics management information system was entirely paper-based (100%). On average, assessments of drug quality conditions and physical damages scored 46.51% and 48.20%, respectively. The overall supply chain maturity at first-level public health facilities is at a marginal capability level (36.35%). While some basic drug supply chain management procedures were in place, they were not consistently followed, and many systems remain manual. The findings underscored significant inconsistencies in the management functions of supplied drugs, with poor adherence to Standard Operating Procedure guidelines.

Consistency in the Assessment of Dried Blood Spot Specimen Size and Quality in U.K. Newborn Screening Laboratories.

In 2015, U.K. newborn screening (NBS) laboratory guidelines were introduced to standardize dried blood spot (DBS) specimen quality acceptance and specify a minimum acceptable DBS diameter of ≥7 mm. The UK 'acceptable' avoidable repeat rate (AVRR) is ≤2%. To assess inter-laboratory variability in specimen acceptance/rejection, two sets of colored scanned images (n = 40/set) of both good and poor-quality DBS specimens were distributed to all 16 U.K. NBS laboratories for evaluation as part of an external quality assurance (EQA) assessment. The mean (range) number of specimens rejected in the first EQA distribution was 7 (1-16) and in the second EQA distribution was 7 (0-16), demonstrating that adherence to the 2015 guidelines was highly variable. A new minimum standard for DBS size of ≥8 mm (to enable a minimum of six sub-punches from two DBS) was discussed. NBS laboratories undertook a prospective audit and demonstrated that using ≥8 mm as the minimum acceptable DBS diameter would increase the AVRR from 2.1% (range 0.55% to 5.5%) to 7.8% (range 0.55% to 22.7%). A significant inverse association between the number of specimens rejected in the DBS EQA distributions and the predicted AVVR (using ≥8 mm minimum standard) was observed (r = -0.734, p = 0.003). Before implementing more stringent standards, the impact of a standard operating procedure (SOP) designed to enable a standardized approach of visual assessment and using the existing ≥7 mm diameter (to enable a minimum of four sub-punches from two DBS) as the minimum standard was assessed in a retrospective audit. Implementation of the SOP and using the ≥7 mm DBS diameter would increase the AVRR from 2.3% (range 0.63% to 5.3%) to 6.5% (range 4.3% to 20.9%). The results demonstrate that there is inconsistency in applying the acceptance/rejection criteria, and that a low AVVR is not an indication of good-quality specimens being received into laboratories. Further work is underway to introduce and maintain standards without increasing the AVRR to unacceptable levels.

Cooling Uncovered Foods at a Depth of ∼5.1 cm (2 in.) or Less Poses Little Risk of Pathogen Growth

The U.S. Food and Drug Administration has guidelines for cooling cooked foods in retail operations. Data on foodborne illness risk factors in restaurants indicate that cooling is often out of compliance with these guidelines. We sought to identify factors under the control of the operator that had a significant effect on the cooling rates of cooked foods. Minneapolis Minnesota Health Inspectors were trained in standardized operating procedures for cooling data collection. Data loggers set to appropriate time intervals and calibrated to ±0.5 °C (∼1°F) were used in data collection. Analysis was performed using the R statistical computing language version 4.2.2. Preexisting pathogen models were used to predict Log CFU increases of Clostridium perfringens or Bacillus cereus. Data from 224 recipes were recorded by inspectors between October 2018 and October 2019. Food depth had a highly significant effect (p = 8.90E−08) on cooling rate. The use of an ice bath or ice wand was also significant (p < 0.005). There was a significant correlation between container material (metal or plastic) and food depth because foods with a greater depth are often being cooled in plastic containers. Foods at a depth greater than 5.1 cm (2 in.) that cooled faster than 0.23 log(°C)/h were often wholly or partially cooled in blast chillers or freezers, cooled using an ice bath or ice wand (or both), or were composed of protein pieces (e.g., chicken wings) that facilitated more rapid cooling due to air gaps in the food. Foods in shallow containers at a food depth of less than or equal to ∼5.1 cm (2 in.) that cooled more slowly than 0.23 log(°C)/h were being cooled at temperatures greater than 5 °C (41°F) or were partially or wholly covered. These foods also showed little evidence of pathogen growth from predictive models. Our analysis shows that cooling foods in shallow containers at a food depth of less than or equal to ∼5.1 cm (2 in.) poses little risk of significant pathogen growth.

Standard operating procedure (SOP) for cervical ultrasound cine loop video sequences in the follow-up of differentiated thyroid carcinoma (DTC).

Cervical ultrasound (US) is crucial in the follow-up of differentiated thyroid cancer (DTC). However, there are no guidelines for its acquisition and documentation, particularly concerning the role of additional video sequences, known as US cine loops (UCL). The aim of this study is to examine the clinical relevance (CR) of a new Standard Operating Procedure (SOP) for cervical UCL in DTC follow-up. A retrospective analysis was conducted on all UCL examinations of DTC follow-up patients at a tertiary care center between January 2010 and February 2018 to determine their clinical significance. The patients were divided into two groups: those with no documented CR (UCL-nCR) and those with documented CR (UCL-CR). The study reviewed the respective written medical US reports that were validated by experienced residents. The UCL-CR were categorized in: confirmation of a suspicious finding that was identified during conventional live US (UCL-CRcon), identification of a suspicious finding that was not identified during conventional live US (UCL-CRide), and invalidation of a suspicious finding that was identified during conventional live US (UCL-CRinv). A total of 5512 UCLs in 652 DTC patients were analyzed, with 71.5% women and a mean age of 50 years. More than 90% of the tumors were low-risk at initial staging. The mean number of UCLs per patient was 8.5 ± 4.6. Overall, 95 cases of UCL-CR were identified in 82 patients (12.6%), with a patient-based number needed to scan of 8. UCL-CRinv was the most common type of UCL-CR, accounting for 77 (81.1%) of cases. The occurrences of 12 UCL-CRcon (12.6%) and 6 UCL-CRide (6.3%) were correspondingly less frequent. The diagnosis of UCL-CR was confirmed in 91.6% of cases during the clinical course. In 12.6% of the patients, the additional acquisition and archiving of cervical UCL revealed clinical relevance in the course of DTC disease. The invalidation of suspicious findings through the retrospective analysis of former UCL occurred as the most significant benefit of this method. The UCL SOP can be easily and quickly integrated into the US workflow.

Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.

Phages are viruses that infect prokaryotes and can shape microbial communities by lysis, thus offering applications in various fields. However, challenges exist in sampling, isolation and accurate prediction of the host specificity of phages as well as in the identification of newly replicated virions in response to environmental challenges. A new workflow using biorthogonal non-canonical amino acid tagging (BONCAT) and click chemistry (CC) allowed the combined analysis of phages and their hosts, the identification of newly replicated virions, and the specific tagging of phages with biotin for affinity chromatography. Replication of phage λ in Escherichia coli was selected as a model for workflow development. Specific labeling of phage λ proteins with the non-canonical amino acid 4-azido-L-homoalanine (AHA) during phage development in E. coli was confirmed by LC-MS/MS. Subsequent tagging of AHA with fluorescent dyes via CC allowed the visualization of phages adsorbed to the cell surface by fluorescence microscopy. Flow cytometry enabled the automated detection of these fluorescent phage-host complexes. Alternatively, AHA-labeled phages were tagged with biotin for purification by affinity chromatography. Despite biotinylation the tagged phages could be purified and were infectious after purification. Applying this approach to environmental samples would enable host screening without cultivation. A flexible and powerful workflow for the detection and enrichment of phages and their hosts in pure cultures has been established. The developed method lays the groundwork for future workflows that could enable the isolation of phage-host complexes from diverse complex microbial communities using fluorescence-activated cell sorting or biotin purification. The ability to expand and customize the workflow through the growing range of compounds for CC offers the potential to develop a versatile toolbox in phage research. This work provides a starting point for these further studies by providing a comprehensive standard operating procedure.

Electrochemotherapy and Calcium Electroporation on Hepatocellular Carcinoma Cells: An In-Vitro Investigation

PurposeElectrochemotherapy, clinically established for treating (sub)cutaneous tumors, has been standardized in the framework of the European Standard Operating Procedure on Electrochemotherapy (ESOPE). Due to common side effects of chemotherapeutic drugs, recent advances focus on non-cytotoxic agents, like calcium, to induce cell death (calcium electroporation). Therefore, this study aims to determine the efficacy of electrochemotherapy with bleomycin or cisplatin, or calcium electroporation on human hepatocellular carcinoma cells (HepG2) in vitro using the ESOPE protocol.MethodsHepG2 cell viability was measured with a MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay after electrochemotherapy with the chemotherapeutic drugs bleomycin or cisplatin (0–20 µM), or after calcium electroporation (0–20 mM), to determine its efficacy on HepG2 cells in vitro using the ESOPE protocol (8 rectangular pulses, 1000 V/cm, 100 µs) compared to non-electroporated drug treatment.ResultsCell viability was significantly lower in electroporated samples, compared to their non-electroporated controls (27–75% difference). Electrochemotherapy with bleomycin and calcium electroporation, reached (almost) complete cell death (− 1 ± 3% and 2.5 ± 2%), in the lowest concentration of 2.5 µM and 2.5 mM, respectively. Electrochemotherapy with 2.5 µM cisplatin, significantly decreased cell viability to only 68% (± 7%).ConclusionElectrochemotherapy with bleomycin or cisplatin, or calcium electroporation were more effective in reducing the HepG2 cell viability in vitro using the ESOPE protocol compared to the non-electroporated drug treatments alone. When comparing electrochemotherapy, HepG2 cells are more sensitive to bleomycin than cisplatin, in similar concentrations. Calcium electroporation has the same effectiveness as electrochemotherapy with bleomycin, but calcium potentially has a better safety profile and several treatment advantages.Graphical

Analisis Disiplin Kerja dalam Meningkatkan Produktivitas Kerja Karyawan Cipta Grafika Karawang

This research aims to determine and analyze the implementation of work discipline in increasing employee work productivity at Cipta Graphic Graha Karawang. The research approach used is qualitative with a descriptive method, which aims to explain the characteristics of the variables that are the focus in analyzing work discipline and employee work productivity at the Cipta Graphic printing press in Karawang. Data collection methods include interviews, observation and documentation studies, with data validity strengthened through triangulation of data sources. The results of the analysis show that work discipline at Cipta Graphic Graha Karawang tends to be good, although there are still several cases of employees arriving late. The main causes include late arrival to work and non-compliance with SOP (Standard Operating Procedures). Efforts to overcome this problem are carried out through the application of sanctions in accordance with regulations and collective agreements, as well as evaluation through recording Error Logs and weekly evaluation meetings to correct work errors that are not in accordance with the SOP (Standard Operating Procedures).

Excruciating existential suffering and complicated grief: The essence of surviving the suicide of a son or daughter.

Suicide is the fourth most common cause of death for the 15-29 age group. Research on the impact of suicide on parents is scarce and, therefore, poorly understood. To explore parents' experiences who have lost a son or a daughter due to suicide and their experience of the services available to them. This phenomenological study involved 1-2 interviews with ten parents aged 40-65, seven mothers and three fathers in all 13 interviews. The age range of their sons and daughters was 17-37 years when they died. For the parents, losing a son or a daughter to suicide is an overwhelming life experience characterised by Excruciating existential suffering and complicated grief where they are confronted with deep meaning-making and existential questions without answers since the person who can answer most of them is no longer alive. They, therefore, felt stuck in their grief for up to 4 years. The initial experience was an immense paralysing shock and sense of unreality. The subsequent period was a blur, and they were numb. Then, their psyche and bodies collapsed, and for a long time, they felt no grief processing was taking place. They sorely needed long-term professional trauma-informed support and felt that, in too many cases, they had to reach out for help themselves. They would have liked to see the healthcare system embrace them with more open arms, offer help and be met with information and individualised support. Standard operating procedures must be installed to support suicide-bereaved parents better. Long-term professional support and trauma-focused care are required following such major trauma, and providing such support could help to reduce their adverse health impacts. Nurses and other health professionals must be better educated on existential suffering in this context.

Implementasi Standar Operasional Prosedur Telepon Operator di Sheraton Hotel Bandung

This research aims to analyze the implementation of standard operational procedures and analyze the obstacles or challenges of telephone operators at the Sheraton Bandung Hotel &amp; Towers. This study uses a qualitative method. By collecting data through observation, interviews, document analysis and field notes, with a focus on the interactions and functions of telephone operators in hotels. Data analysis uses narrative analysis to produce in-depth descriptions of telephone operators' experiences and interaction patterns. The data reduction process simplifies and filters information to find the most relevant and significant findings. Interpretation of findings is carried out by connecting them with relevant theory or literature, and the results of the analysis are explained in detail in the research report. The results of this research indicate that the implementation of Standard Operating Procedures (SOP) for telephone operators at the Sheraton Bandung has been carried out successfully, which has had a positive impact on service performance and customer satisfaction. However, despite this, several areas were identified that needed to be improved to achieve a better level of consistency. The challenges and obstacles faced, both technical and non-technical, need to be resolved in order to increase overall service effectiveness.

Implementasi Kebijakan Peraturan Walikota Surabaya Nomor 106 Tahun 2022 Tentang Tata Cara Pengumpulan, Pengolahan dan Pemanfaatan Data Keluarga Miskin: Studi di Kecamatan Krembangan Kota Surabaya

This research aims to find out the extent of the implementation of Perwali Policy number 106 of 2022 in the City of Surabaya. This research uses descriptive qualitative research methods. The communication contained in policy implementation is two-way communication from the Social Service to the community and to the relevant SKPD in the Surabaya area. Resource factor, from this indicator it can be seen that the existing resources in the sub-districts, Surabaya City Social Service, RW and RT in the Krembangan District area include staff, information, authority and facilities which are quite good. Disposition factor, from this indicator it can be concluded that the disposition carried out by the sub-district in Krembangan District and the Surabaya City Social Service includes cognition, direction and response which is quite good with employee responses being quite responsive. From this indicator it can be concluded that the sub-districts in Krembangan District, the Surabaya City Social Service, RT and RW in the Krembangan District area have implemented Standard Operating Procedures (SOP) for the implementation of data collection on the Poor (GAKIN).

A standard operating procedure for prehospital anaesthesia and its effect on mortality-An observational study.

Prehospital anaesthesia is a complex intervention performed for critically ill patients. To minimise complications, a standard operating procedure (SOP) outlining the process is considered valuable. We investigated the implementation of an SOP for prehospital anaesthesia in helicopter emergency medical services (HEMS). We performed a retrospective observational study of patients receiving prehospital anaesthesia by Finnish HEMS from January 2012 to August 2019. The intervention studied was the implementation of an SOP at two of the five bases during 2015-2016. Patients were stratified according to whether they were anaesthetised before, during or after implementation and the primary outcomes were 1- and 30-day mortality. Secondary outcomes included anaesthesia quality indicators. Confounding factors was assessed via logistic regression. A total of 3902 tracheal intubations were performed without an SOP, 430 during implementation and 1525 after implementation. The SOP had a significant effect on 1-day mortality during implementation with an odds ratio (OR) of 0.56, 95% confidence interval (95% CI) 0.37-0.81 and a further trend towards benefit after implementation (OR 0.84, 95% CI 0.68-1.04), but no difference in 30-day mortality (OR after implementation 1.10, 95% CI 0.92-1.30). Implementation of an SOP improved first-pass success rate from 87.3% to 96.5%, p < 0.001. Implementation of an SOP for prehospital anaesthesia was associated with a trend towards lower 1-day mortality and an improved first-pass success but did not affect 30-day mortality. Despite this, we advocate prehospital systems to consider implementation of a prehospital anaesthesia SOP as immediate performance markers improved significantly.

Internal Audit Guide to the K3 Management System at Simpang Lima Gumul Hospital Kediri

Hospitals in the BLUD system play an optimal role in providing health services, including the implementation of hospital operations using income sources and each part of the hospital must be able to have its own role and function but are interconnected to fully support smooth operations. The problem of a company or hospital related to occupational safety and health in general is about the readiness of management and all employees to face risks that may occur before, during and after the production process or health services with the aim of increasing effective, efficient productivity and reducing losses due to the risks incurred. Therefore, it is necessary to carry out an internal audit regarding SMK3. The main target is the management of Simpang Lima Gumul Hospital Kediri. The aim of this activity is to find out the Standard Operational Procedures for SMK3 Internal Audit at Simpang Lima Gumul Hospital Kediri. The method used in this activity is presentation regarding SMK3 Internal Audit Standard Operating Procedures to the management of Simpang Lima Gumul Kediri Hospital using power point as a medium for supplying material. The results of this activity are Standard Operational Procedures for Internal Audit of SMK3 Simpang Lima Gumul Hospital Kediri.

Optimization of Outpatient Waiting Times to Improve the Achievements of National Quality Indicators at TNI AD 05.08.04 Hospital Lawang

The waiting time for outpatient services in a hospital is one of the important factors determining patient satisfaction with the hospital, so it is hoped that patients will not wait too long while in the hospital. This study aimed to optimize outpatient waiting times so that it can increase the achievement of national quality indicators at TNI AD 05.08.04 Hospital, Lawang. This study used fishbone diagrams, USG (Urgency, Seriousness, Growth), and SWOT Analysis (Strength, Weakness, Opportunity, Treats) to formulate problems and find solutions. The results of this study showed that priority problems in waiting time include: standard operating procedure (SOP) for outpatient service flow are still not available, the electronic medical record system is still often slow, and evaluation of national quality indicators is still not carried out routinely. Furthermore, the proposed strategy offered is the creation of SOP regarding the flow of outpatient services and routine outreach regarding the use of electronic medical records. With the implementation of these two strategies, it is hoped that they will be able to speed up waiting times for outpatient services, thus improving the achievement of national quality indicators.

Proposal for a qualification system for New Approach Methodologies (NAMs) in the food and feed sector: example of implementation for nanomaterial risk assessment

Abstract Plenty of new approach methodologies (NAMs) for risk assessment have been developed but only some are included in OECD Test Guidelines (TGs) for regulatory implementation. Nevertheless, NAMs are increasingly applied, e.g. for nanomaterial (NM) risk assessments. The EFSA Guidance on NM risk assessment suggests that NAM‐derived data concerning degradation/dissolution (in relevant biofluids), intestinal uptake/crossing, genotoxicity, cytotoxicity, oxidative stress, (pro‐)inflammatory potential and barrier integrity, for many of which no OECD TGs exist, have to be evaluated first. Consequently, NM risk assessments involve data from non‐guideline studies, requiring time‐consuming and challenging case‐by‐case evaluations. Establishing an OECD TG is a formal process aiming for international use according to the Mutual Acceptance of Data (MAD). However, not every promising NAM can be prioritised for OECD TGs. A qualification, based on an expert opinion, may enable an efficient use of adequate NAMs for a specific context‐of‐use. Furthermore, it supports the optimisation of promising NAMs for regulatory applications. Existing qualification systems operate in the context of e.g., drug development tools (FDA) and research and development into pharmaceuticals (EMA). The NAMS4NANO consortium was tasked to propose a generic framework for a qualification system for chemical risk assessment in the food and feed sector to speed up the regulatory use of NAMs. Here we describe our proposal including the process and evaluation criteria. A detailed test method description, preferably as standard operating procedures (SOPs), describing the set‐up of the NAM including its application and evaluation phase is crucial. Furthermore, the scientific validity, i.e. its reliability and relevance for the context‐of‐use, needs to be demonstrated, for which we suggest a less rigorous process compared to OECD TGs. We propose to initially establish a qualification system for NM risk assessment, aligned with the EFSA framework. This document is an interim version to stipulate a broader discussion among experts and stakeholders.

Discussion on the enhancement of Typhoon Committee activities for UN EW4All initiative

The ESCAP/WMO Typhoon Committee is an intergovernmental regional organization dealing with typhoon (tropical cyclones) related disasters. Millions of people around the Asia-Pacific region remain exposed to a higher frequency and intensity of natural hazards. For more developed countries, tropical cyclone related impacts cause major social and economic disruptions through loss of lives and property. With recognizing the importance of aligning its efforts with UN Early Warnings for All (EW4All) initiative which aims to strengthen early warning systems globally, Typhoon Committee resolved to initiate the monitoring of ongoing or past efforts by TC Members in contributing to the four key pillars of the EW4all initiative aiming to (1) evaluate the Committee's contributions to EW4All; and (2) identify the opportunities for enhancing performance through Member Reports and the WGs’ AOP specification. TC had its 18th IWS/4th TRCG forum with a theme of “Early Warnings for All Through Enhancement of Typhoon Standard Operating Procedures (SOP)” and “Towards a Typhoon Resilient Society”; TC Secretary (TCS) initiated a reviewing and monitoring on the ongoing or previous efforts of TC Members, through analyzing the AOPs’ implementation of TC WGs, in contributing to the pillars of the EW4All initiative with the objective of enabling an evaluation of the Committee's contributions to EW4All and identify opportunities for further enhancing its performance. This paper intends to discuss the directions on how to further enhance TC activities aligning with and contribution to EW4All in future.

Occupational exposure protection and perioperative management of sudden infectious diseases from the perspective of COVID-19.

The coronavirus disease (COVID-19) SARS-CoV-2 virus epidemic continues to exhibit a sporadic onset trend due to the continuous variation of the novel coronavirus. However, the psychological impact of the pandemic persists. It is crucial to reflect on our experiences to better prepare for future large-scale infectious diseases. During outbreaks of infectious diseases, patients may still require orthopaedic surgery. It is crucial to prioritize the safety of medical staff and establish procedures to ensure their protection. However, with the implementation of a series of standardized operational protection procedures, orthopaedic surgeons can safely perform their duties without the risk of contracting COVID-19. There is no doubt that the orthopaedic occupational exposure protection process and perioperative management plan for global infectious diseases, such as COVID-19, require a standardized summarization process and a narrative review.

Juridical Review of the Phrase "Unlawful" in Decisions on Corruption Crimes in State-Owned Legal Entities

In several court decisions in cases of criminal acts of corruption in State-Owned Enterprises (SOE), the phrase "against the law" is in Article 2 paragraph 1 of Law Number 31 of 1999 concerning the Eradication of Corruption Crimes as amended based on Law Number 20 2001 concerning Amendments to Law Number 31 of 1999 concerning the Eradication of Corruption Crimes (Anti Corruption Act) is interpreted broadly by referring to the explanation of these regulations. Because it relates to an offense, the phrase "against the law" should be interpreted by taking into account the principle of legality, the principle of nullum delictum nulla poena sine previa lege poenali. The use of a broad interpretation of the phrase "against the law" results in unclear differences between unlawful acts in the context of civil law and (onrechtmatige daad) and unlawful acts in the context of criminal law (wederrechtelijkheid). This results in the risk of criminalization of SOE officials, giving rise to fear in making business decisions. The formulation of the problem in this research is how to interpret the phrase "against the law" in article 2 paragraph (1) of the Corruption Law when it is related to cases of criminal acts of corruption in SOE and what is the judge's interpretation of the phrase "against the law" in decisions court for criminal acts of corruption in SOE. The research method used is a research method with a normative juridical approach. The results of the research show that the phrase "against the law" is interpreted broadly in the explanation of article 2 paragraph (1) of the Corruption Law, and judges in several corruption crime decisions interpreted the phrase "against the law" broadly, resulting in violations of the Standard Operating Procedure, Work Plan and The Company's Articles and other internal company regulations are interpreted as unlawful acts that can be considered a criminal act of corruption.

Establishing a Standard Operating Procedure for Fertility Preservation in Adolescent and Young Adult Male Survivors of Childhood Cancers: Addressing a Gap in Care in the Republic of Ireland

Establishing a Standard Operating Procedure for Fertility Preservation in Adolescent and Young Adult Male Survivors of Childhood Cancers: Addressing a Gap in Care in the Republic of Ireland

Towards a Standardized Operating Procedure for eDNA‐Based Biomonitoring in Coastal Marine Salmon Aquaculture

ABSTRACTThe marine aquaculture industry and regulators are in the process of implementing environmental DNA (eDNA) metabarcoding of microbial communities for compliance monitoring. This requires standardization of sampling, laboratory, and data analysis protocols. Towards this goal, we in this study completed two further milestones using samples collected from two Scottish salmon farms: (i) We tested the effect of using two different PCR protocols (i.e., different DNA polymerases, master mixes, and annealing temperatures), which are frequently being used in eDNA biomonitoring of aquaculture installations, for the amplification of the taxonomic marker gene (V3‐V4 hypervariable region of the bacterial 16S rRNA gene). (ii) We quantified sampling background noise obtained from eDNA samples and statistically compared results with the sampling bias observed in macrofaunal samples from the same source sediments. We detected differences in bacterial community structures resulting from the performance of different PCR protocols, profoundly influencing the interpretation of biomonitoring results. Furthermore, we found that sampling‐induced errors for eDNA samples were similar to errors for macrofaunal samples collected according to compliance monitoring protocol (~25% variability in both cases). Finally, we showed that within‐grab variances of microbial community structures were in the same order of magnitude (less than 10× difference in all cases) as the one obtained from replicate grabs collected from the same locale (impact category). Based on our findings, we suggest using a consistent PCR protocol for biomonitoring efforts to improve the comparability of results, especially when different service providers are conducting the biomonitoring. We propose a sampling scheme to be considered in eDNA biomonitoring that includes taking three replicate grabs at each locale, with one replicate sample from each grab. This minimizes sampling‐induced errors and makes upcoming eDNA‐based monitoring results comparable with previous compliance monitoring results obtained from macrofaunal data.