Standard Laboratory Methods Research Articles

Comparative Effects of Neem-coated Urea, Agrotain Urea and Nano Nitrogen on Nutrient Uptake of Paddy Crop

Aims: To evaluate the impact of innovative nitrogen management strategies, including neem-coated urea (NCU), nano fertilizers, and Agrotain urea, on nutrient use efficiency (NUE), NPK uptake, and rice productivity in the Southern Telangana Zone. Study Design: Randomized block design with 3 replications, including traditional and innovative nitrogen formulations combined with foliar-applied nano nitrogen sprays. Place and Duration of Study: Agricultural Research Institute, Rajendranagar, during the Kharif season of 2021&2022. Methodology: The experiment was conducted on the JGL 24423 rice variety. Treatments included varying nitrogen rates (100%, 50%, and 33% recommended dose of nitrogen [RDN]) applied as NCU (Neem Coated Urea), Agrotain UG (urea granules), or combinations with nano nitrogen sprays. Nitrogen was applied in three splits (transplanting, tillering, and panicle initiation). Nutrient uptake (NPK) was analyzed using standard laboratory methods, and statistical analysis was performed using ANOVA to assess treatment effects. Results: The 100% RDN-STCR (Soil Test Crop Response)-based NCU treatment demonstrated the highest mean nitrogen uptake (66.8 kg ha⁻¹ in grain, 53.7 kg ha⁻¹ in straw), phosphorus uptake (18.7 kg ha⁻¹ in grain, 9.5 kg ha⁻¹ in straw), and potassium uptake (37.7 kg ha⁻¹ in grain, 120.9 kg ha⁻¹ in straw). Reduced nitrogen treatments with nano nitrogen sprays showed moderate nutrient uptake.The control and nano nitrogen sprays alone had the lowest uptake levels. Conclusion: Nitrogen management strategies, particularly 100% RDN-STCR-based NCU, significantly enhanced NUE, nutrient uptake, and rice productivity, demonstrating their potential for sustainable rice cultivation.

Assessing the Impacts of Coal Mining on Soil Quality in Kogi East, Nigeria

This study investigates the effects of coal mining on soil physical and chemical properties in Kogi East, Nigeria, focusing on two mined sites (Ika-Ogboyaga and Okaba) and one unmined control site (Abache). Soil samples were collected from 32 plots across mined areas and 16 plots in the unmined site using a 200m x 200m belt transect method. Samples were analyzed for particle size, bulk density, soil pH, organic matter, nitrogen, phosphorus, exchangeable cations, and heavy metal concentrations using standard laboratory methods. Results show minimal differences in soil texture (sandy clay loam) across all sites, with slightly higher bulk density in mined areas (1.89±0.2g/cm3, 1.87±0.6 g/cm3), indicating soil compaction from mining activities. Chemical properties, including pH, organic carbon, nitrogen, and cation exchange capacity, showed minor variations, while heavy metal concentrations (Zn, Cu, Fe, Pb) were within safe limits across all sites. Analysis of Variance (ANOVA) (F-value=0.00449; F-crit=3.1907; P-value=0.995511) showed no significant differences in most soil properties between mined and unmined areas, suggesting limited impact of coal mining on soil quality in the region. To improve soil health and sustainability the study recommended mitigating soil compaction, implementing vegetation rehabilitation with native species, conducting continuous soil monitoring, raising community awareness, and promoting sustainable mining practices.

Evaluation of factors associated with immunoglobulin, protein, fat and lactose concentrations in colostrum of dairy cows from Austria.

Calves rely on the passive transfer with immunoglobulins derived from colostrum. Currently, there is a lack of knowledge on colostrum management practices and colostrum quality on small scale family-owned dairy farms in Austria. The objectives of this study were to describe factors that are associated with immunoglobulin, protein, fat and lactose concentrations in dairy cow colostrum from the federal state of Salzburg. Therefore, an online questionnaire was designed to gather information on general farm characteristics. Further, the farmers collected individual colostrum samples and completed a detailed accompanying questionnaire for each sample. Immunoglobulin levels were determined by using a Brix refractometer and protein, fat and lactose by standardized laboratory methods. Linear mixed effect models were built to test factors associated with colostrum immunoglobulin, fat, protein and lactose concentrations. In total, 1,050 colostrum samples from 72 dairy farms were collected. The number of calvings per year was distributed as follows: ≤10 calvings: 8.3% of the farms, 11 to 20: 31.9%, 21 to 30: 29.2%, 31 to 40: 15.3% and ≥ 41 calvings: 15.3%. Overall, the median Brix value was 22.0% (7.3-36.1%). The number of samples with good and poor-quality colostrum was 517 and 528, respectively. Cow-level factors significantly affecting colostrum Brix% were parity, calving season, ante partum colostrum leakage, time lag between parturition and colostrum collection. In total, a subset of 307 colostrum samples from 39 farms from pure-breed dual-purpose Simmental cows were further analysed for protein, fat and lactose concentration. The median concentration for fat was 5.1% (0.5-18.5%), protein 14.6% (4.2-27.5%) and lactose 2.3% (0.2-5.0%). The cow-level factors affecting protein concentration were similar to the factors influencing Brix%. Fat concentration was influenced by the time lag between calving and colostrum collection and by parity. The present study confirmed the factors, which are currently known to have an impact on colostrum quality. This was the first large scale approach in the federal state of Salzburg to survey colostrum management including colostrum sample collection. The range of colostrum quality was wide (7.3% Brix to 36.1% Brix) therefore many calves will be at risk of receiving poor quality colostrum as defined by a Brix of ≤ 22%.

Investigation of Liver Function Test Findings in COVID-19 Patients Considering the Disease Timeline

Background: Several lines of evidence suggest that SARS-CoV-2 infection impacts liver function; however, the evaluation of these changes over time according to the disease timeline remains unclear. Objectives: The objective of this study was to identify potential alterations in liver function parameters during the first to fifth waves of COVID-19 and compare these alterations with the demographic and clinical outcomes of patients across the waves. Methods: This cross-sectional study included 1501 hospitalized patients with a confirmed RT-PCR diagnosis of COVID-19, referred to Shahid Sadoughi Hospital, Yazd, Iran, between November 20, 2019, and November 20, 2021. Liver function parameters, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, and albumin, were assessed using standard clinical laboratory methods. Statistical analyses were conducted to evaluate differences in liver function parameters across the five pandemic waves, with significance set at P < 0.05. Results: The median age of the hospitalized patients (N = 1501) was 61 ± 21.8 years, with the majority being male (816, 54.3%). The Kruskal-Wallis test showed a significant increase in the median levels of ALP, total bilirubin, and direct bilirubin in the first wave; the AST and ALT in the third wave; and serum albumin in the second wave (P < 0.001). The median serum levels of total bilirubin and direct bilirubin were significantly higher in older patients compared to younger patients (P < 0.001), and the median ALT and serum albumin were significantly lower in older patients (P < 0.0001). In males, the median values of AST, ALT, total bilirubin, and direct bilirubin were significantly higher than in females (P < 0.0001). The median values of all parameters, except serum albumin, in deceased patients were significantly higher compared to discharged patients, and serum albumin was significantly lower. Conclusions: In this descriptive study, we observed that older and male patients were more frequently hospitalized than females. Our findings showed variations in liver parameters across different pandemic waves, suggesting distinct behaviors of various SARS-CoV-2 strains.

Glucose Testing Methods: A Systematic Review and Meta-Analysis of Diagnostic Accuracy of Point-of-Care Devices for Neonatal Hypoglycemia.

To evaluate the accuracy of various point-of-care device methodologies for measuring blood glucose concentrations in babies at risk of neonatal hypoglycemia. This systematic review and meta-analysis included studies from Ovid MEDLINE, Ovid Embase, and Web of Science up to May 20, 2024. Studies comparing point-of-care testing methods for neonatal blood glucose to a standard laboratory method were included, excluding those on continuous glucose monitoring or conducted before 1990. Two researchers independently assessed inclusion and evaluated risk of bias using QUADAS-2. Sensitivity and specificity were calculated using contingency tables, and diagnostic accuracy was analyzed using hierarchical random-effects modelling. Studies with insufficient data were summarized by estimation direction. Seventy-one studies were included. The quantitative analysis (n = 31) evaluated glucose oxidase (GO) + photometry (n = 8), glucose-1-dehydrogenase (GDH) + photometry (n = 6), GO + electrochemistry (n = 13), GDH + electrochemistry (n = 12), and hexokinase (HK) + electrochemistry (n = 2). All methods showed high specificity (≥93%), with GO + electrochemistry, GDH + electrochemistry, and HK + electrochemistry showing superior sensitivity. The SROC curve confirmed HK + electrochemistry as the most accurate. Certain point-of-care device methodologies demonstrate greater accuracy in measuring neonatal blood glucose concentrations. Of the methods evaluated, HK + electrochemistry proved to be the most reliable. However, the limited number of studies using this method suggests the need for further research to confirm these findings across diverse settings and populations.

Assessing Asphalt Binder Aging with 1H Spin-lattice NMR Relaxometry: A Comparative Study of Temperature and UV Radiation Effects

Objective: Asphalt binder, a byproduct of the crude-oil refining process, is commonly used in road construction due to its favorable response to physical stresses. However, it is also susceptible to environmental aging, including temperature variations and ultraviolet (UV) radiation. Standard laboratory methods for studying asphalt binder aging primarily focus on factors like temperature, pressure, and oxygen, often neglecting the impact of UV radiation. This study employs 1H spin-lattice nuclear magnetic resonance (NMR) relaxometry to investigate the effects of UV radiation on asphalt binder aging compared to temperature-induced aging. Methods: Primary relaxation times and ratios were derived from a bi-exponential fit of the experimental relaxation decay induced by a 200-MHz NMR magnet. NMR relaxometry analysis revealed that 90% (0.9 primary ratio) of the protons in unaged asphalt binders exhibited a characteristic primary spin-lattice relaxation time (T1) of approximately 470ms. Results: Cold exposure did not significantly alter these parameters. Mimicked heat aging, which considered only temperature and duration, resulted in slight increases in both the primary relaxation time and ratio. In contrast, after 72h of UV exposure, binders showed variable T1 values at a significantly decreased primary ratio. This variability was summarized with the new performance parameter, the Herbaw. Further testing showed that standardized heat aging, which considered sample size and pressure, reduced both the primary relaxation time and ratio when compared to the mimicked aging. Conclusion: These results demonstrate that UV radiation induces significant structural changes in asphalt binder, which can be effectively detected using spin-lattice NMR relaxometry. The Herbaw performance parameter was successful in describing the variability of the UV aging which greatly impacted the primary ratio, indicating oxidative stress. The Herbaw Parameter was not as successful in describing heat aging since only slight changes in the primary relaxation time were detected. While the hydrogen environments were not significantly impacted, temperature-based aging may depend on multiple mechanisms.

Resting metabolic rate and energy efficiency in response to an intensive 84-day combat-swimmer training in the German Armed Forces

Abstract Purpose According to the ‘constrained model’, there are compensations in resting metabolic rate (RMR) at high levels of physical activity (PA). Here, we have used a standardized combat-swimmer training protocol (CST) to investigate whether changes in RMR (i) confirm the ‘constraint model’, and (ii) differ between successful participants and dropouts. Methods Controlled 84d CST in 44 male soldiers with 13 finally successful. Fat mass (FM) and fat-free mass (FFM) were measured using Quantitative Magnetic Resonance. RMR was assessed by indirect calorimetry, VO2max, and work efficiency by treadmill spiroergometry. Plasma levels of thyroid hormones, testosterone, and cortisol were analysed by standard laboratory methods. Results CST increased VO2max (+ 6.9%) and exercise efficiency at low workloads of 10 and 12 km/h (+ 8.7 and + 6.5%; both p < 0.05). As energy balance was moderately negative (−356 ± 383 kcal/d), FFM and FM decreased (−2 and −16%; both p < 0.05). There was a considerable inter-individual variance but no change in in the mean values of RMR and RMRadjFFM. RMRadjFFM before CST had a negative association with its decrease with CST (p < 0.005). Concomitantly, plasma hormone levels were unchanged. When compared with dropouts, successful participants had a higher VO2max at baseline (5.2 ± 0.6 vs. 4.9 ± 04 l/min; p < 0.05) that increased with CST (+ 4.4 vs. −0.4%; p < 0.05) at similar changes in body composition and energy balance. Conclusion While CST increased VO2max and exercise efficiency as a compensation, there was an inter-individual variance in exercise-related compensation of RMR with no differences between ‘completers’ and ‘non-completers’. Trial registration DRKS00018850, November 27, 2019.

Hydrochemical Dynamics and Water Quality Assessment of the Ramsar-Listed Ghodaghodi Lake Complex: Unveiling the Water-Environment Nexus

Human activities and climate change increasingly threaten wetlands worldwide, yet their hydrochemical properties and water quality are often inadequately studied. This research focused on the Ghodaghodi Lake Complex (GLC) and associated lakes in Nepal, a Ramsar-listed site known for its biodiversity and ecological significance. The study was conducted to assess seasonal water quality, investigate the factors influencing hydrochemistry, and assess the lakes’ suitability for irrigation. Forty-nine water samples were collected from the GLC in pre-monsoon and post-monsoon periods. Nineteen physicochemical parameters, such as dissolved oxygen (DO), total dissolved solids (TDS), and major ions (calcium ‘Ca2+’, magnesium ‘Mg2+’, and bicarbonate ‘HCO3−’), were analyzed using standard on-site and laboratory methods. Statistical methods, including analysis of variance (ANOVA), T-tests, and hydrochemical diagrams, e.g., Piper, were adopted to explore spatial and seasonal variations in water quality, revealing significant fluctuations in key hydrochemical indicators. Results showed marked seasonal differences, with pre-monsoon TDS levels averaging 143.1 mg/L compared to 78.9 mg/L post-monsoon, underscoring evaporation and dilution effects. The hydrochemical analysis identified Ca2+-HCO3− as the dominant water type, highlighting the influence of carbonate weathering on GLC’s water composition. Gibbs, mixing, and Piper diagram analysis supported these findings, confirming the predominance of HCO3−, with Ca2+ and Mg2+ as the main cations. Additionally, sodium adsorption ratio (SAR) values were consistently below 1, confirming excellent irrigation quality. These findings provided critical data for policymakers and stakeholders, supporting sustainable wetland management and aligning with the United Nations’ Sustainable Development Goals relevant to environmental conservation, i.e., clean water and life on land.

Digital SERS immunoassay of Interleukin-6 based on Au@Ag-Au nanotags

Interleukin-6 (IL-6) is a crucial cytokine involved in inflammation and immune regulation. However, the detection of IL-6 with ultrasensitivity and high specificity remains a significant challenge due to the inherent complexity of biofluids. Herein, we present a digital surface enhanced Raman scattering (SERS) immunoassay using core-shell Au@Ag-Au nanotags for IL-6 detection with ultrasensitivity and high reliability. A low-cost silicon chip was functionalized as capture substrates, employing novel SERS nanotags that exhibit strong, robust and reproducible signals at single-nanoparticle resolution as the amplification element. We proposed two analytical methods to validate single-molecule events follow a Poisson distribution and to quantify protein biomarkers over a broad linear dynamic range, respectively. The strong alignment between theoretical and experimental results enhances the method's reliability. Our assay provides two readouts: colorimetric analysis by naked eyes for high concentrations (>1 ng/mL) and digital SERS analysis for low concentrations. Following method optimization, we obtained a linear range from 100 fg/mL to 1 ng/mL (R2 = 0.994) with a limit of detection (LOD) of 12.4 fg/mL, suitable for clinical applications. The method was tested for IL-6 quantification in healthy human serum and saliva, with recoveries from 92.4% to 105.3%. Finally, the immunoassay demonstrated strong consistency with the standard clinical laboratory method when tested with clinical serum samples. Thus, our proposed the digital SERS immunoassay is a promising tool for the precision clinical diagnosis of IL-6-related diseases or other conditions.

Association of uremic toxins and systemic inflammation with depression and anxiety among hemodialysis patients in Montenegro.

The purpose of this study was to examine the association between uremic toxins, inflammation, depression and anxiety among hemodialysis patients in Montenegro. The cross-sectional study included 88 patients undergoing hemodialysis. Depression and anxiety symptoms were assessed by Patient Health Questionnaire (PHQ-9) and Beck Anxiety Inventory (BAI). The standard laboratory methods were used to measure uremic toxins and systemic inflammation (C-reactive protein, Neutrophil-to-Lymphocyte Ratio (NLR) and Platelet-to-Lymphocyte Ratio (PLR)). Clinically significant depression (PHQ-9 ≥ 10) and anxiety (BAI ≥16) were found in 55.7% and 27.2%, respectively. Logistic regression analyses revealed that serum urea, beta 2-microglobulin (B2M), and systemic inflammation (CRP, NLR, PLR) were significantly and independently associated with depressive symptoms, whereas serum urea, creatinine, uric acid, B2M, and systemic inflammation (CRP nad NLR) were associated with anxiety symptoms. Depressive symptoms and anxiety are common among hemodialysis patients in Montenegro, and are associated with increased levels of systematic inflammation, serum urea, and beta 2-microglobulin.

Vitamin D Status and Oxidative Stress in Children with Sickle Cell Anaemia in Sagamu, Nigeria

Objectives: Sickle cell anaemia (SCA) is characterized by nutritional deficiencies and oxidative stress. Vitamin D possesses antioxidant properties but its role in SCA in sub-Saharan Africa has not been fully understood. The relationship between Vitamin D status, oxidative stress and antioxidants status in children with SCA was investigated for its possible role in reducing complications arising from oxidative stress in SCA. Methods: Case-control study involving 100 HbS genotype and 100 HbA genotype children (control) of comparable age (5-12 years). Baseline characteristics were obtained and serum vitamin D, calcium, CAT, SOD, GPX, GST and XO levels were quantified by standard laboratory methods. Results: Serum levels of vitamin D, calcium, CAT, SOD, GPX and GST were significantly lower in SCA group compared to the control, while the XO level was significantly higher in the HbS group compared with control. There was positive correlation between vitamin D, CAT and SOD (r = 0.821, 0.869), weak positive association between vitamin D and Ca (r = 0.545) and no significant relationship between vitamin D and other measurands in the SCA group. Conclusion: Sufficient vitamin D status might impact positively on the antioxidant status in SCA individuals thereby reducing associated complications.

Hepatocellular Changes on Paracetamol Induced Liver Damage in Long Evans Male Rats upon Green Tea Administration

Background: The liver is a vital organ that serves a number of purposes in our body. It may be harmed by pollutants, substances with toxic effects, and long-term, unchecked drug use. Green tea is a well-liked beverage that has gained popularity recently and may have hepatoprotective properties. Objective: To observe the effect of green tea (Camellia sinensis) on paracetamol induced liver damage in Long Evans male rats. Methods: From July 1st, 2018, to June 30th, 2019, this study was conducted in the Department of Physiology at Sir Salimullah Medical College (SSMC), Dhaka. For the purpose of the study, thirty (30) male Long Evans rats, weighing between 150 and 200 grams and 90 to 120 days old, appeared to be in good health. They were split into two groups after 14 days of acclimatization: Group A, which served as the control group, and Group B, which served as the experimental group (green tea pretreatment and paracetamol treated). Group A1 (baseline control group) and group A2 (paracetamol treated control group) comprised the subdivided control group. There were ten rats in each of these groups. For 28 days, the rats were all fed a baseline diet. The baseline control group was given normal saline (20 ml/kg/day) orally every day for 28 days in addition to their basal diet. For the final three days of the study—the 26th and 28th—the paracetamol-treated control group was given oral paracetamol at a dose of 1.5 g/kg/day. During the final three days of the study period (the 26th and 28th days), the experimental group was given oral paracetamol (1.5g/kg/day) and an ethanolic extract of green tea (500 mg/kg/day) for a total of 28 days. On day 29, every rat was sacrificed. Following the sacrifice, liver samples were taken. Hepatic contents of malondialdehyde (MDA) were measured by using standard laboratory method. Histopathology of liver was also done by using standard laboratory procedure in the department of pathology, SSMC. Version 22 of the Statistical Package of Social Science (SPSS) for Windows was used to do the statistical analysis. The data were displayed as mean±SD. To compare the results, one-way ANOVA, post hoc Bonferroni, and Chi-square tests were used, where appropriate. A p value of less than 0.05 was regarded as significant. Result: When compared to the baseline control group, the mean malondialdehyde concentration in the liver was significantly (p) greater in the green tea pretreatment, paracetamol treated, and control groups who received paracetamol treatment. Once more, the groups treated with green tea and paracetamol had mean malondialdehyde concentrations in their livers that were significantly (p) lower than those of the paracetamol group. Additionally, 0% of the rats in the baseline control group, 100% of the rats in the paracetamol-treated group, and 30% of the rats in the green tea-pretreated and paracetamol-treated group had abnormal histological findings of the liver. Conclusion: This study revealed that green tea has hepatoprotective effect against paracetamol induced liver damage in Long Evans male rats. Bangladesh Crit Care J September 2024; 12 (2): 145-152

Sevelamer Treatment for Hyperphosphatemia in a Saudi Arabian Chronic Renal Disease Patient

Abstract Introduction/Objective Introduction: Patients with advanced chronic kidney disease (CKD) often require phosphate binders to manage elevated serum phosphorus levels due to phosphorus-restricted diets being insufficient. This study aimed to assess the safety and efficacy of sevelamer in lowering serum phosphorus and improving the serum lipid profile in a hemodialysis patient. Methods/Case Report A prospective study was conducted on a 59-year-old hemodialysis man with CKD. Sevelamer was administered orally as 800 mg tablets, with the dosage adjusted based on washout serum phosphorus levels (WSPL). Blood samples were collected every two weeks before dialysis, and all samples were analyzed using standard clinical laboratory methods. Sevelamer treatment resulted in a significant reduction in serum phosphorus levels from 6.9 mmol/l to 4.3 mmol/l. No major adverse events were observed during the trial period, and there was no evidence of negative consequences associated with sevelamer therapy. Additionally, sevelamer treatment led to a decrease in hemoglobin levels to 10.5 g/dl and a substantial reduction in total cholesterol concentration from 5.22 mmol/l to 4.26 mmol/l. Results (if a Case Study enter NA) NA Conclusion Conclusion: Sevelamer, a non-absorbable aluminum and calcium-free phosphate binder, effectively lowered serum phosphorus levels in dialysis patients while improving lipid profiles. It was well tolerated with no instances of hypercalcemia and resulted in significant reductions in intact parathyroid hormone (PTH) and total and LDL cholesterol. Sevelamer may serve as a valuable therapeutic option for managing hyperphosphatemia in patients with CKD.

Ecotoxicity of plastic leachates on aquatic plants: Multi-factor multi-effect meta-analysis

Despite heightened awareness of plastic contamination, a comprehensive understanding of the ecotoxicity of plastic leachates remains challenging due to discrepancies in previous findings and complexities in the effects of myriad factors. Herein, we proposed a multi-factor multi-effect plastic-leachate ecotoxicology meta-analysis approach (PLEM) to elucidate the ecotoxicity of plastic leachates on aquatic plants. To distinguish the leachate toxicity from the general effects of leachates and plastic particles, the previous studies on the effects of leachate stricto sensu (i.e., without particles) were exclusively encompassed. A total of 890 data points explored in 18 previous articles were systematically analyzed. Our findings revealed that plastic leachates negatively affected aquatic plants' growth (31 %) and photosynthesis (13 %). These toxic effects were influenced by multifaced factors including plastic characteristics, leaching conditions, and plant species. Polyvinyl chloride leachates exhibited the highest toxicity among different polymers. Marine species showed greater susceptibility than freshwater species. Surprisingly, leachates from centimeter-sized plastics exhibit higher toxicity than those from nanometer, micrometer, and millimeter-sized plastics. These findings underscore the toxicity of plastic leachates on aquatic plants should be more systematically assessed using standardized laboratory methods and considering multi-factors. This study offers a valuable insight into the toxic mechanism of plastic leachates and plastic contamination.

Validating a portable device for groundwater iron measurement in Bangladesh

The mineral iron is essential for health at an optimum level. In Bangladesh iron is obtained from the drinking source groundwater, attributing largely to the good iron status in population. This groundwater iron poses two scenarios—predominantly high in many areas and predominantly low in other areas. Despite good iron status, burden of anemia in population is high. To control anemia, iron supplementation programs are operational against the backdrop of high iron status. This often leads to iron-associated side effects, plausibly due to excess iron, especially among the individuals exposed to high amount of iron from the drinking water. This geological phenomenon warrants the supplementation program to deliver different doses depending on the groundwater iron level. To enable that, the country needs an area-representative “mapping” of groundwater iron. However, such a mapping using the gold standard laboratory methods is expensive and logistically intensive. The aim of the study is to validate Handheld Colorimeter (HI721 Checker® HC), a portable device (“spot kit” device) to measure iron concentration in the drinking groundwater. The study was carried out in the rural Belkuchi upazila of the Sirajganj district in the Rajshahi division. In the study 25 tubewells were selected and categorized into five groups. Among them, four were categorized based on their iron concentration, low iron conc. (0-<1 mg/L), medium iron conc. (1-2 mg/L), high iron conc. (2-10 mg/L), very high iron conc. (>10 mg/L). One additional group was included for tubewell water that was used after filtering. The study compared the iron estimates measured in groundwater samples of the selected tube wells by the spot kit device and the same samples measured by atomic absorption spectrophotometry (AAS). There was a high agreement between the estimates by the spot kit and AAS (Kappa coefficient= 0.72, p <0.001; Lin’s concordance = 0.829, p < 0.001). Pitman's test of difference in variance exhibited that the two methods produced equally precise results (r=0.227, p=0.275). The study concluded that the Handheld Colorimeter (HI721 Checker® HC) was valid to measure the iron concentration of groundwater under field conditions. This device can facilitate testing processes, particularly in remote or resource-constrained areas where sophisticated testing methods (i.e: AAS) are unavailable and thus may help the re-design of the anemia control program in settings like Bangladesh with high and variable level of iron in the drinking groundwater.

Effectiveness of the use of chickpea collection samples in breeding

Aim. To determine the breeding value the large number of collection chickpea (Cicer arietinum L.) varieties obtained from the International Crops Research Institute for the Semi-Arid Tropics (India, Patancheru, ICRISAT) and the National Center for Plant Genetic Resources of Ukraine (Kharkiv) was evaluated in 1995-2022 under the conditions of the steppe zone of Ukraine. Methods. Standard field and laboratory methods (phenological observations, hybridization, structural analysis, biochemical analysis of protein content in seeds according to the Kjeldahl method), statistical analysis. Results. Donors and sources of such economically valuable features as early maturity, increased productivity, protein content in seeds, large-seeded, resistance against pathogens on an artificially created infectious background were identified. The set of 12 varieties of chickpea were developed by artificial hybridization, which are recommended for cultivation in all zones of Ukraine. Conclusions.The characteristics of the created varieties are given, the most valuable collection genotypes for use in crossing programs are recommended.

A Comparative Evaluation of Point-of-Care and Laboratory HbA1c Testing in Diabetes Care: An Indian Perspective.

This study aimed to assess the diagnostic performance of a point-of-care (POC) glycated hemoglobin (HbA1c) device in the Indian population against the standard laboratory (high-performance liquid chromatography {HPLC}) method for the effective management of diabetes in India. This study on the diagnostic accuracy of a POC HbA1c device involved 121 participants. These participants were categorized into two groups according to their HbA1c levels - one group with HbA1c values below 6.5% and another group with HbA1c values equal to or greater than 6.5%. The HbA1c levels in enrolled participants were estimated using both the POC device (HemoCueHbA1c 501; Ängelholm, Sweden: HemoCue AB) and the standard HPLC-based method. The level of agreement and concordance between the two test results were assessed by the Bland-Altman plot and Lin's concordance correlation coefficient. Sensitivity, specificity, diagnostic accuracy, positive likelihood, and negative likelihood ratios of POC-HbA1c device were assessed with a 6.5% HbA1c cut-off. The mean HbA1c values obtained by the two methods showed no statistically significant difference with a minimum effect size (Cohen's d = 0.035), indicating there was a negligible difference between these methods. The Bland-Altman plot revealed that most values were within acceptable limits (95% CI: -0.5 to 0.7) and Lin's concordance correlation coefficient showed strong agreement (p < 0.0001).The POC-HbA1c device demonstrated an area under the curve (AUC) of 0.991 (95% CI: 0.953-1.000) with sensitivity, specificity, diagnostic accuracy, positive likelihood, and negative likelihood ratios of 93.62%, 97.30%, 95.87%, 34.64 and 0.07, respectively, compared to the standard diagnostic assay. The diagnostic accuracy, sensitivity, and specificity demonstrated by the POC-HbA1c device to the standard HPLC method offers a viable and practical solution for diabetes management in India. Its ability to provide rapid and reliable results at the point of care can improve patient outcomes, reduce healthcare costs, and enhance access to diabetes care, especially in primary care, remote areas, and resource-limited settings of developing countries like India.

Distributed wireless IoT based sensing and quality monitoring system in protection of wetlands groundwater areas

Abstract This paper presents the development and implementation of a sensor network based on the Internet of Things (IoT) to monitor key groundwater quality parameters with the goal of minimizing potential risks in the protection of biodiversity. These parameters include the water level, water temperature, pH, conductivity, and dissolved oxygen. The system prioritizes scalability, ease of use, real-time data acquisition and minimal power consumption, resulting in efficient and dependable water quality monitoring with the convenience of remote applications. The continuous measurement system was set up to be a single source of information for monitoring groundwater quality in protected wetland areas across international borders, including Tompojevacki Ritovi (Municipality of Tompojevci, Croatia) and Lake Zobnatica (Municipality of Backa Topola, Serbia). The findings of two years of Wireless Sensor Network (WSN) monitoring were compared to standard laboratory methods to demonstrate the accuracy and precision of WSN readings. Appropriate calibration and installation of the WSN give a larger volume of data and thus grow the database, allowing for a more accurate identification of water contamination and a quick response in the event of pollution. Smart IoT-based sensors help to protect water quality and, as a result, the well-being of ecosystems and human communities, which is especially important in protected areas like wetlands.&amp;#xD;

Combined Lactate Glucose Ratio as a Novel Marker for Rapid Diagnosis of Cerebrospinal Fluid Bacterial Infection in Neurosurgical Patients: Diagnostic Accuracy Study and Benchtop Analyzer Correlation

ObjectiveThis study aimed to assess the diagnostic accuracy of a novel marker, the combined lactate-to-glucose ratio (CLGR), in identifying cerebrospinal fluid (CSF) bacterial infection (CBI) in neurosurgical patients. Additionally, it seeks to establish cut-off values for CLGR and evaluate the reliability of measurement using blood gas analyzer (BGA). MethodsCSF samples were collected from two neurosurgical centers in Kuala Lumpur, Malaysia, between January 2022 and October 2023. Conventional markers and CLGR were quantified using standard laboratory methods, with BGA utilized for measurement when feasible. Samples were categorized into confirmed CBI-positive (CBI+) and CBI-negative (CBI-) groups. Marker performance was compared, and receiver operating characteristic analysis conducted. Pearson's correlation assessed the agreement between BGA and laboratory measurements. ResultsAmong the 130 CSF samples, 11 were CBI+. Both cLac and CLGR were significantly elevated in the CBI+ group (p<0.001). The area under the curve (AUC) for cLac and CLGR was 0.990 and 0.994, respectively. Using a cut-off of 6.0mmol/L, cLac demonstrated sensitivity of 100%, specificity of 93.3%, positive predictive value (PPV) of 57.9%, negative predictive value (NPV) of 100%, and diagnostic accuracy of 93.9%. CLGR ≥20.0 showed even higher accuracy: 100.0% sensitivity, 98.6% specificity, 84.6% PPV, 100% NPV, and overall accuracy of 98.5%. Both markers maintained excellent performance in blood-stained CSF. BGA measurements correlated well with laboratory results (r=0.980 & 0.999, respectively, p<0.001). ConclusionsCLac levels ≥6.0mmol/L and CLGR ≥20.0 accurately identified CBI in neurosurgical patients, with CLGR exhibiting superior efficacy. The potential for instant BGA measurement suggests promise for point-of-care testing.

Soil Chemical and Physical Properties in Taungya and Non-Taungya Plots at Sao Hill Forest Plantation, Tanzania

This study compared soil physical and chemical properties in Tungya and Non-Taungya plots at Sao Hill Forest plantation, Tanzania. Soil samples were collected from 0-20cm depth in February and May Using a zigzag pattern to ensure representative coverage. Physical properties (bulk density, porosity, Soil moisture content, soil texture) and chemical properties (soil pH electrical conductivity, total nitrogen and available phosphorous) were analyzed using standard laboratory methods. Results showed significant differences between the two management practices. In February Non-Taungya plots had higher bulk density(1.33±0.151/cm3) compared to Taungya plots (1.16±0.107 g/cm3; p&lt;0.001), while Taungya plots showed higher porosity (56.12%±4.06p&lt;0.001). May sampling also revealed differences with non-Taungya plots maintaining higher bulk density (1.32±0.15 g/cm3) compared to Taungya plots (1.08±0.09 g/cm3); p&lt;0.001). Soil texture analysis showed no significant differences in sand content between treatments. However, silt content was significantly higher in Taungya plots (13.27 ± 3.849%) compared to non-Taungya plots (10.69 ± 3.23%; p=0.007), while clay percentage did not differ significantly. Soil chemical properties, non-Taungya plots had higher pH (4.67±0.47) compared to Taungya plots (4.3±0.22; p&lt;0.001) while electrical conductivity (0.04±0.04 dS/m) compared to Taungya plots (0.01±0.01 dS/m; p&lt;0.001). Taungya plots showed higher total nitrogen (0.04±0.01%; p&lt;0.0031) compared to non-Taungya plots (0.035±0.01%). In additional May available phosphorous in Taungya plots was (3.95±0.55 mg/kg; p&lt;0.001) compared to non-Taungya plots (1.97±0.32 mg/kg). These results suggest that Taungya practices significantly influence soil properties, potentially impacting soil quality and forest productivity. The study provides valuable awareness for sustainable forest management and highlights the need for long-term monitoring of soil properties under different management systems in plantation forests