Abstract Background: Hospital stays, mortality, and expenditures rise with health-care-associated infections, especially in developing countries. Public health promotes infection control. Aim: The aim of this study was to assess health-care workers’ (HCWs) infection control knowledge, attitude, and practice and determine how sociodemographic and job descriptive variables affect them. Settings and Design: A cross-sectional study at four Baghdad/Al-Rusafa government hospitals (Shaikh Zayed Hospital, Al-Shaheed Al-Sader Hospital, Ibn Al-Balady Children and Maternity Hospital, and Fatima Al-Zahraa Children and Maternity Hospital). Subjects and Methods: All resident doctors, nurses, and laboratory personnel who were present during data collection and agreed to participate were included in this study. Statistical Analysis: Data were entered using Excel 2019 and then analyzed using SPSS 26. Frequency and percentage analyzed quantitative data, while Chi-square test analyzed categorical data. P <0.05 indicated statistical significance. Results: Knowledge level was higher among HCWs aged 30–39 years (79.4%), doctors (81.0%), individuals with more than 5 years of experience (75.0%), and those who received training (83.7%). Positive attitudes were most prevalent among HCWs aged 49 years and older (68.6%), laboratory workers (59.4%), individuals with more than 5 years of experience (55.5%), and those who received training (60.6%). A higher level of practice was observed in individuals aged 30–39 years (83.2%), laboratory personnel (80.4%), those with 5–14 years of experience (80.4%), and trainees (83.0%). Conclusions: Three-quarters of HCWs had good knowledge and practice regarding infection control precautions, and nearly half of them had a positive attitude. The knowledge, attitude, and practice of HCWs were increasing with progress in age, increasing work experience, and also with training.
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