Background The practice of taking standard precautions for infection control in designated microscopy centres for sputum examination is important to prevent the transmission of Tuberculosis to not only to the laboratory personnel but also to the general population.Objectives To assess the perception amp compliance to infection control practices among lab technicians of Designated Microscopy Centres DMC in Davangere tuberculosis unit.Methodology It was carried out among all the lab techniciansrsquo working in DMCs coming under Davangere TU. A pretested semi-quantitative questionnaire was used to know the practices towards infection control among LTs. Data was entered in MS Excel 2016 and analyzed in SPSS v16.0. Results Among the 08 DMCs visited majority of the lab technicians were males 62 and their mean duration of service was 12.12 - 6.46 years. The majority of the lab technicians had good knowledge of infection control practices but poor in infection control practices.