Background: Urinary tract infection (UTI) is One of the most prevalent infectious diseases in human, where the study aimed to identify the most uropathogen that caused UTIs ,and its the antimicrobial resistance pattern.
 Methods: the samples were collected from January and December 2021 using a standard data collection form, where the patient outcome records were mined for information on the patients' gender, age, isolated organisms, and susceptibility test results, then statistical analysis was done.
 Results: 266 from 463 samples were identified as S. aureus 14.3% , in addition, the antibacterial resistance rats showed that the highest resistance was against ampicillin/Sulbactam, ceftazidine, and meropenem 79%, while, the lowest was against ciprofloxacin 40%.
 Conclusion: The study showed that S. aureus is the primary cause of UTIs in Derna city, as a result of consuming antibiotics randomly, which resulted in an increase in spread and resistance of S. aureus.
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