This study explores the impact of tungsten inert gas (TIG) welding parameters on the mechanical properties and microstructure of dissimilar welds between AISI 304 and AISI 316 austenitic stainless steels. Given the growing industrial demand for these materials, the research focuses on optimizing welding current, shielding gas flow rate, and voltage to enhance tensile strength, hardness, and impact toughness. Using the L9 orthogonal array based on Taguchi’s methodology, the experiments revealed that Relatively highs currents and voltages significantly improved the ultimate tensile strength (up to 673.67 MPa) and impact energy absorption (up to 36.5 J). Microstructural analysis indicated refined grain structures in the heat-affected zones, with pronounced grain growth in AISI 316 due to its thermal sensitivity. The micro-hardness analysis revealed that the highest hardness occurred in the HAZ of AISI 304, with samples 5 and 6 exhibiting the optimal hardness profile, reflecting the most favorable welding parameters. The study concludes that a current range of 80-90 A and a voltage range of 10-11 V and a shielding gas flow rate of 12-16 L/min provide optimal welding conditions, offering robust guidelines for industrial applications requiring high-performance dissimilar welds.