In a typical Nb <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> Sn cable for fusion applications, the knowledge of the strain state of Nb <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> Sn filaments is important to predict and interpret the conductor performance in operation. Amongst the relevant strain sources, the predominant is the thermal strain that arises during the cool-down of the conductor to operating temperature, due to the different expansion of Nb <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> Sn and copper in the cable and the stainless steel conduit. This work addresses the characterization of Nb <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> Sn thermal strain in the React & Wind prototype (RW2) developed by EPFL-SPC for the toroidal field coil and the central solenoid of the EUROfusion DEMO fusion reactor. AC susceptibility measurements were performed on the RW2 prototype to assess the thermal strain distribution in the cable cross-section, before and after undergoing electromagnetic and thermal cyclic loading. The results of the analysis have shown that RW2 exhibits a significantly lower absolute mean strain value (about −0.3%), compared to many Wind & React ITER conductor samples investigated with the same method. In addition, the analysis highlighted that the assessed thermal strain distribution does not vary significantly before and after the electromagnetic and thermal cycles, confirming the small performance degradation in the RW2 conductor that has been recently reported.