Aluminum alloy bolt-sphere joint is one of the major nodes in aluminum alloy space grid structures. It has been applied in many big projects. In this study, computation methods of mechanical properties and bearing capacity of aluminum alloy bolt-sphere joints in fire are proposed by combining experimental research and theoretical analysis. Results demonstrate that: 1) The tensile strength of aluminum alloy bolt-sphere joints in fire is related to the tensile strength of stainless-steel bolt and the tensile strength seal plate joint. 2) The stainless-steel bolt develops two failure modes, namely, breakage failure of cord and pulling-out failure of screw in fire. 3) The seal plate joint in fire has three tensile failure modes, namely, tensile-shearing combined failure of seal plate at the annular compression position, shear failure of seal plate, and tensile failure of the aluminum alloy tube at the seal plate border. 4) The tensile strength of the stainless-steel bolt begins to decrease significantly when the temperature reaches 300°C. 5) The tensile strength of the seal plate joint begins to decrease sharply when the temperature reaches 200°C. 6) The formula to calculate bearing capacity of aluminum alloy bolt-sphere joints in fire is proposed based on experimental results.