Stages Of Cycle Research Articles

DEVELOPMENT OF CLASSIFIERS OF DEVIATIONS FROM THE REQUIREMENTS OF REGULATORY DOCUMENTS OF THE OIL AND GAS INDUSTRY AND COMPENSATORY MEASURES

The paper analyzes the problem of ensuring safety at various stages of the life cycle of main pipelines and steel vertical tanks in cases of deviations from the requirements of current design standards. To effectively solve this problem, independent and easily modifiable classifiers of deviations from the requirements of regulatory documents, as well as measures aimed at compensating for these deviations, have been developed. The faceted method was chosen as the classification method, which allows us to divide a large object of study into smaller characteristics independent of each other, which facilitates the decision-making process and allows us to take into account many aspects.The resulting classifiers are a tool for systematization of information obtained during the analysis and development of special technical conditions necessary to ensure the safety of main pipelines and steel vertical tanks. Due to their ability to be easily modified, they can be adapted to specific objects and conditions, which makes them a versatile and flexible tool for developing and making engineering decisions.An important aspect of the work is the practical testing of the developed classifiers by applying them to a real object of the oil and gas industry. This stage of testing demonstrated the high efficiency and practical applicability of the proposed classifiers, which indicates their potential aimed at improving the safety of oil and gas transportation and storage facilities, as well as products of their processing.In addition, the systematization of information on deviations from the requirements of regulatory documents and ways to compensate for them allows not only to increase the level of safety during the construction, reconstruction and operation of pipelines and reservoirs, but also to reduce the consequences of possible emergencies.The results obtained represent an important step in the development of an approach to ensuring the safety of main pipelines and steel vertical tanks, and also have both practical and scientific value, and can be recommended for use by specialists in the field of design, technosphere safety and the development of special technical conditions.

Introducing the Occupational Health and Safety Potential Midpoint Impact Indicator in Social Life Cycle Assessment

Occupational health and safety (OSH) is crucial for sustainable development, recognized by corporations, the European Union, and Sustainable Development Goals. This study introduces a characterization model for OSH in the social life cycle assessment (S-LCA) to support the quantification of OHS along product supply chains and sustainable decision making. The characterization model aims to provide a practical approach for assessing OHS at the product level with actual working hours or recommends a secondary approach with monetary data, when working hours are unavailable, to calculate the Occupational Health and Safety Potential (OHSP). The developed model was tested in a theoretical case study on shirt production in Europe and globally. The case study shows that the European shirt value chain resulted in higher OHSP values than the global shirt values chain. In addition, the model shows which life cycle stages and organizations highly contributed to the OHSP results. In both approaches, the shirt production stage contributed highly. Differences in results emerged based on the calculation approach, underscoring the model’s versatility, because increasing the complexity of calculating the CFs with monetary values will affect the results based on sectorial monetary output. Additionally, the study mentions benefits to the operationalization of social impact assessment and limitations when the developed characterized model is employed. Last, this study aids in offering a tool for organizations to meet the demands of the new Corporate Sustainability Reporting Directive by quantifying and publicizing OHS data.

Problems of creating an effective system of health technology assessment at the early stages of the drug life cycle

Aim. To study modern approaches to the formation and functioning of the Horizon Scanning (HS) system and early health technology assessment (HTА), in particular, for the treatment of rare diseases with significant unmet medical needs. Materials and methods. The analysis, systematization and synthesis of data from scientific publications, analytical reports, guidelines, information from official websites of regulatory authorities, HTА and HS agencies were used. Results. The analysis of the theoretical foundations and regulatory support for the processes of forming an effective HS system at the early stages of the life cycle has been performed. HS systems, the so-called “early awareness and alert systems”, are aimed at identifying, filtering and prioritizing new and innovative technologies with a significant predicted impact on health, costs, society and the healthcare system in order to inform policy makers, purchasers and healthcare providers or improve early access. The HS system allows for proactive planning and decision-making on the use and reimbursement of new medicines based on preliminary assessments of budgetary impact and clinical efficacy. Conclusions. For the national HTA system, which currently is at the stage of development, it is important to adapt positive international experience in this area and provide functionality for HS, early assessment, early dialogue, managed access with the development of the evidence base. It is advisable to apply the “light” model and join the international cooperation in this area.

Abstract PO4-03-01: Enhancing Breast Cancer Subtyping: APIS Breast Cancer Subtyping Kit Proliferative Signature's Potential for Improved Proliferation Assessment and Recurrence Prediction

Abstract Background: Ki67, a widely recognized biomarker for measuring tumor cell proliferation in breast cancer (BC), plays a crucial role in classifying BC into luminal A and luminal B subtypes, subsequently aiding treatment decisions. However, the inconsistency in Ki67 assessment and the lack of standardization in methods and interpretation of immunohistochemistry (IHC) staining has hindered its recommendation for routine diagnostic use. The standardization of measurement techniques, especially for Ki67, holds immense potential for improving patient treatment outcomes. The APIS Breast Cancer Subtyping Kit (APIS BCS Kit) addresses these challenges by utilizing RT-qPCR to accurately determine the mRNA expression of BC markers, including Ki67, along with a novel four-gene proliferative signature, comprising four markers (MKI67, CCNA2, KIF23, and PCNA) associated with proliferation and expressed throughout all cell cycle stages, thereby providing a more accurate measure of tumor proliferation. In this study, we present the preliminary findings of our ongoing evaluation of the APIS BCS Kit proliferative signature's ability to distinguish between Luminal A and Luminal B subtypes and to determine the risk of recurrence in hormone receptor-positive (HR+) patients. Method: A total of N=153 retrospectively collected HR+/HER2 negative samples were provided by University of Basel. For each sample the following clinical annotations were included: relapse free survival, treatment regimen (Chemotherapy (ChT) or Hormone Therapy (HT)) as well as OncotypeDx (ODx) risk score (RS). N=40 samples underwent additional testing using PAM50 (Prosigna®) test. Only samples collected since 2018 were included, resulting in 10 observed relapse events (N=7 in HT cohort and N=3 in ChT cohort). RNA isolation from tissue sections and gene expression analysis were performed as per APIS BCS Kit instructions for use. The agreement of subtype calls between IHC, APIS BCS Kit, and PAM50 was assessed. The correlation between RS obtained from ODx, PAM50 and the APIS BCS Kit proliferation signature was also explored. Results: The agreement between IHC and the APIS BCS Kit subtype call, utilizing Ki67 alone as a measure of proliferation, was 67.3% (103/153). Replacing Ki67 with the APIS BCS Kit proliferation signature increased the concordance to 69.9% (107/153). The concordance between IHC and PAM50 subtype calls was comparable at 62.5% (25/40). The agreement between the APIS BCS Kit and PAM50 subtype call was notably higher (80%; 32/40). Significant association was observed between the APIS BCS Kit proliferation signature and ODx RS (P=.0001), and PAM50 RS (P=.002). In the HT cohort, both ODx and the APIS BCS Kit proliferation signature accurately predicted recurrence (by assigning high RS) in n=2 cases. In some cases both the APIS BCS Kit and ODx erroneously assigned low risk and subsequently missed n=4 recurrence events. Notably, APIS BCS Kit proliferation signature correctly identified one recurrence event (high RS), which was missed by ODx (low RS). Conclusion: The APIS Breast Cancer Subtyping Kit exhibits improved agreement with the PAM50 molecular subtype in contrast to the IHC-derived subtype, indicating that molecular testing may be a more suitable method for determining proliferation and distinguishing between luminal A and B subtypes. Furthermore, there is a significant correlation between the RS generated by APIS Breast Cancer Subtyping Kit proliferation signature and both PAM50 and Oncotype RS. These initial findings indicate that the APIS Breast Cancer Subtyping Kit proliferation signature has the potential to accurately identify patients at risk of recurrence, although additional studies including a larger proportion of events are needed to validate these results. Citation Format: Marcus Vetter, Anna Gasior, Elena-Diana Chiru, Joanna Gorniak, Leanne Gough, Mathew Harrison, Sara Rollinson, Simone Muenst, Christian Kurzeder. Enhancing Breast Cancer Subtyping: APIS Breast Cancer Subtyping Kit Proliferative Signature's Potential for Improved Proliferation Assessment and Recurrence Prediction [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO4-03-01.

Scorpion Venom Antimicrobial Peptide Derivative BmKn2-T5 Inhibits Enterovirus 71 in the Early Stages of the Viral Life Cycle In Vitro.

Enterovirus 71 (EV71), a typical representative of unenveloped RNA viruses, is the main pathogenic factor responsible for hand, foot, and mouth disease (HFMD) in infants. This disease seriously threatens the health and lives of humans worldwide, especially in the Asia-Pacific region. Numerous animal antimicrobial peptides have been found with protective functions against viruses, bacteria, fungi, parasites, and other pathogens, but there are few studies on the use of scorpion-derived antimicrobial peptides against unenveloped viruses. Here, we investigated the antiviral activities of scorpion venom antimicrobial peptide BmKn2 and five derivatives, finding that BmKn2 and its derivative BmKn2-T5 exhibit a significant inhibitory effect on EV71. Although both peptides exhibit characteristics typical of amphiphilic α-helices in terms of their secondary structure, BmKn2-T5 displayed lower cellular cytotoxicity than BmKn2. BmKn2-T5 was further found to inhibit EV71 in a dose-dependent manner in vitro. Moreover, time-of-drug-addition experiments showed that BmKn2-T5 mainly restricts EV71, but not its virion or replication, at the early stages of the viral cycle. Interestingly, BmKn2-T5 was also found to suppress the replication of the enveloped viruses DENV, ZIKV, and HSV-1 in the early stages of the viral cycle, which suggests they may share a common early infection step with EV71. Together, the results of our study identified that the scorpion-derived antimicrobial peptide BmKn2-T5 showed valuable antiviral properties against EV71 in vitro, but also against other enveloped viruses, making it a potential new candidate therapeutic molecule.

Dendroclimatology of Araucaria angustifolia: assessing climatic responses in sites under similar ecological conditions

Dendrochronological studies have increasingly explored tropical and subtropical trees, mainly focusing on their climatic signals. In the neotropics, growth ring width chronologies of Araucaria angustifolia have been developed at several localities in the south of its distribution, in the Atlantic forest biome. However, the signals identified in these studies indicate different patterns of growth response to climate. Understanding the causes of this variability in dendroclimatic signals is important to guide future dendrochronological studies with A. angustifolia. In this work we test the premise that at independent sites, but under similar environmental conditions, chronologies of A. angustifolia exhibit a common growth signal determined by limiting climatic conditions. We developed a new ring width chronology and correlated it with another one already published, both located in Curitibanos municipality, Santa Catarina state, Brazil and with similar climatic, edaphic and vegetational conditions. The chronologies, with reasonable level of internal growth synchrony and sampling effort, showed a common growth signal of moderate magnitude. This common growth pattern between the sites, represented by a regional chronology, showed significant correlations with local climatic variable and with El Niño Southern Oscillation (ENSO), with weak magnitudes. Partial correlations showed that this set of climatic variables was determinant of the common signal between the sites. Our results confirm the validity of the tested premise. Under the conditions studied, the growth response of A. angustifolia to climate is driven by yearly variation in temperature, which operate at different stages of the annual growth cycle and are influenced by ENSO. The low sensitivity of growth to climate is possibly related to the combined influence of multiple climatic factors and/or to moderate growth variability within sites. The multiplicity of climatic factors influencing growth of A. angustifolia trees may also explain some variability of climatic signals reported for with this species.

Infertility in POMC-deficient mice

The pro-opiomelanocortin (POMC) gene is expressed in the hypothalamus and pituitary and its product is cleaved into several peptides including the melanocyte stimulating hormones (α, β, or γ-MSH), adrenocorticotropic hormone (ACTH), and beta-endorphin. Alpha-MSH is involved in regulating appetite, sexual behavior, and melanin, while ACTH regulates secretion of glucocorticoids from the adrenal cortex. Humans who have a POMC mutation either produce an abnormally short version of POMC or are missing the protein completely and therefore lack these hormones. This has biological consequences including red hair and fair skin, early-onset obesity (due to severe hyperphagia), and potentially hypothyroidism, hypogonadism, and infertility. Many of these same effects are seen with mice containing this deficiency. We are interested in studying infertility through a mouse model of hypothalamic-specific POMC-deficiency. These mice also experience early-onset obesity and infertility. Estradiol is a key reproductive hormone that is essential for fertility. POMC deficiency may lead to hypogonadism, which can cause low levels of estrogen so we hypothesize that POMC-deficient mice have reduced estrogen levels, which may contribute to their infertility. To test this hypothesis, we will measure estrogen levels in POMC-deficient mice at different stages of the estrus cycle using an estradiol ELISA kit. We expect the POMC-deficient mice to have lower estrogen levels than control mice. We also plan to use liquid chromatography mass spectrometry (LC-MS) to measure estrogen levels. Before collecting the blood samples, vaginal cell samples are obtained and viewed under a microscope to analyze the ratio of cells and determine which stage the females were in—proestrus, estrus, metestrus, or diestrus. Blood samples are then collected into 1.5 mL microcentrifuge tubes with 10 microliters of 0.5 M EDTA to prevent blood clotting. After blood is collected, it is centrifuged at 2,000 g for 20 minutes at 4 degrees Celsius. The plasma is then stored at -80 degrees Celsius until analysis. Based on preliminary results we expect the POMC deficient mice to have reduced estrogen and/or altered levels throughout the cycle. So far, our results have indicated that POMC-deficient female mice have abnormal estrus cycles and also reduced uterine weights. The conclusions from this study will help us understand more about the causes of infertility in POMC-deficient mice, which may also lead to greater understanding on how to treat humans with infertility due to a POMC mutation. Support for this research was provided by IRACDA grant NIHK12GM111725 to Z.T. and UVU Scholarly Activities Grant to A.G. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

The political ecology of justice outcomes of lithium mining: The case of Salar del Hombre Muerto, Argentina

Electromobility and lithium-ion batteries are seen as key for the global energy transition, but injustices have been identified in several stages of the life cycle of electric vehicles, particularly in the resource extraction stage. Little is known about the micropolitics driving the acceptance or contestation of lithium mining by communities directly affected by this activity in the highland Andes of South America. In this paper, we explore complaints and demands made by communities living near Salar del Hombre Muerto, a salt flat in the Argentinian province of Catamarca where lithium has been extracted for decades. Having collected ethnographic data from conversations with affected communities and during public meetings and hearings, we applied a coding process using political ecology and energy justice conceptual frameworks. This led to the identification of place-based complaints and demands associated with distributive, procedural, and recognition justice. We further discuss our findings in terms of recent literature on lithium mining to provide explanations of the justice outcomes identified. Although lithium mining may not be the sole cause of (at least some of) these injustices, it nevertheless generates expectations and catalyzes attempts to end marginalization in areas where local and regional institutions are incapable of doing so.

Life Cycle of Precipitating Cloud Systems from Synergistic Satellite Observations: Evolution of Macrophysical Properties and Precipitation Statistics from Geostationary Cloud Tracking and GPM Active and Passive Microwave Measurements

Abstract Observations of clouds and precipitation in the microwave domain from the active dual-frequency precipitation radar (DPR) and the passive Global Precipitation Measurement (GPM) Microwave Imager (GMI) onboard the GPM Core Observatory satellite are used in synergy with cloud tracking information derived from infrared imagery from the GOES-13 and Meteosat-7 geostationary satellites for analysis of the life cycle of precipitating cloud systems, in terms of temporal evolution of their macrophysical characteristics, in several oceanic and continental regions of the tropics. The life cycle of each one of the several hundred thousand cloud systems tracked during the 2-yr (2015–16) analysis period is divided into five equal-duration stages between initiation and dissipation. The average cloud size, precipitation intensity, precipitation top height, and convective and stratiform precipitating fractions are documented at each stage of the life cycle for different cloud categories (based upon lifetime duration). The average life cycle dynamics is found remarkably homogeneous across the different regions and is consistent with previous studies: systems peak in size around midlife; precipitation intensity and convective fraction tend to decrease continuously from the initiation stage to the dissipation. Over the three continental regions, Amazonia (AMZ), central Africa (CAF), and Sahel (SAH), at the early stages of clouds’ life cycle, precipitation estimates from the passive GMI instrument are systematically found to be 15%–40% lower than active radar estimates. By highlighting stage-dependent biases in state-of-the-art passive microwave precipitation estimates over land, we demonstrate the potential usefulness of cloud tracking information for improving retrievals and suggest new directions for the synergistic use of geostationary and low-Earth-orbiting satellite observations.

A New Cyclic Cohesive Zone Model for Fatigue Damage Analysis of Welded Vessel

A new cyclic cohesive zone fatigue damage model is proposed to address the fatigue problem spanning high and low cycle stages. The new damage model is integrated with the damage extrapolation technique to improve calculation efficiency. The model's effectiveness in regulating the low-cycle fatigue evolution rate, overall fatigue damage evolution rate, and stress level at the fatigue turning point is assessed through the comparison of the S-N curves. The fatigue damage model's high precision is proved based on the minor deviation of stress at the turning point of the S-N curve from the actual scenario. Finally, the fatigue damage evolution is simulated considering the effects of pre-load pressure and welding residual stress. It is observed that laser welding induces a significant residual tensile stress, accelerating fatigue damage evolution, while compressive loading impedes fatigue damage progression.

Investigating Social and Environmental Hotspots Throughout the Lifecycle of Product-Service Systems in the Early Design Stages

Product-Service Systems (PSS) have been recognized as potential game-changers for advancing sustainability. However, the sustainability of a PSS over traditional products and services is not automatic; it requires careful, intentional design. Despite a decade's progress in relevant literature, early-stage design assessment of a PSS – particularly from a life cycle standpoint and considering the social sustainability dimension – remains underexplored. This study introduces a framework for early-stage identification and analysis of potential environmental and social impacts within a PSS’s life cycle to aid in decision-making and guide towards sustainable solutions. The framework's hotspot analysis, which combines life cycle assessment techniques, aims to pinpoint life cycle stages most susceptible to significant environmental and social impacts. Drawing on existing literature and expert evaluations, this approach offers designers preliminary insights into potential impacts, establishing a groundwork for comprehensive life cycle analysis once more detailed information on products, services, and organizational structures becomes available. The hotspot analysis not only addresses the lifecycle view of a PSS but also places emphasis on the oft-neglected social dimension of sustainability, marking an area ripe for further study. By supporting decision-making in the early design phase and encouraging scrutiny of various PSS concepts, this approach promotes the cultivation of sustainably-designed solutions.

CDK1-PP2A-B55 interplay ensures cell cycle oscillation via Apc1-loop300

Cell cycle control relies on a delicate balance of phosphorylation with CDK1 and phosphatases like PP1 and PP2A-B55. Yet, identifying the primary substrate responsible for cell cycle oscillations remains a challenge. We uncover the pivotal role of phospho-regulation in the anaphase-promoting complex/cyclosome (APC/C), particularly through the Apc1-loop300 domain (Apc1-300L), orchestrated by CDK1 and PP2A-B55. Premature activation of PP2A-B55 during mitosis, induced by Greatwall kinase depletion, leads to Apc1-300L dephosphorylation, stalling APC/C activity and delaying Cyclin B degradation. This effect can be counteracted using the B55-specific inhibitor pEnsa or by removing Apc1-300L. We also show Cdc20's dynamic APC/C interaction across cell cycle stages, but dephosphorylation of Apc1-300L specifically inhibits further Cdc20 recruitment. Our study underscores APC/C's central role in cell cycle oscillation, identifying it as a primary substrate regulated by the CDK-PP2A partnership.

Distinct life cycle stages of an ectosymbiotic DPANN archaeon.

DPANN archaea are a diverse group of microorganisms that are thought to rely on an ectosymbiotic lifestyle; however, the cell biology of these cell-cell interactions remains largely unknown. We applied live-cell imaging and cryo-electron tomography to the DPANN archaeon Nanobdella aerobiophila and its host, revealing two distinct life cycle stages. Free cells possess archaella and are motile. Ectobiotic cells are intimately linked with the host through an elaborate attachment organelle. Our data suggest that free cells may actively seek a new host, while the ectobiotic state is adapted to mediate intricate interaction with the host.

Quantifying cell cycle regulation by tissue crowding

The spatiotemporal coordination and regulation of cell proliferation is fundamental in many aspects of development and tissue maintenance. Cells have the ability to adapt their division rates in response to mechanical constraints, yet we do not fully understand how cell proliferation regulation impacts cell migration phenomena. Here, we present a minimal continuum model of cell migration with cell cycle dynamics, which includes density-dependent effects and hence can account for cell proliferation regulation. By combining minimal mathematical modeling, Bayesian inference, and recent experimental data, we quantify the impact of tissue crowding across different cell cycle stages in epithelial tissue expansion experiments. Our model suggests that cells sense local density and adapt cell cycle progression in response, during G1 and the combined S/G2/M phases, providing an explicit relationship between each cell-cycle-stage duration and local tissue density, which is consistent with several experimental observations. Finally, we compare our mathematical model’s predictions to different experiments studying cell cycle regulation and present a quantitative analysis on the impact of density-dependent regulation on cell migration patterns. Our work presents a systematic approach for investigating and analyzing cell cycle data, providing mechanistic insights into how individual cells regulate proliferation, based on population-based experimental measurements.

Determining directions for transforming the organization's competence structure depending on its life cycle stage in the context of enabling effective operation in the market

Determining directions for transforming the organization's competence structure depending on its life cycle stage in the context of enabling effective operation in the market

Differential effects of high-fat diet on endocrine, metabolic and depressive-like behaviors in male and female rats

The complications of obesity extend beyond the periphery to the central nervous system (CNS) and include an increased risk of developing neuropsychiatric co-morbidities like depressive illness. Preclinical studies support this concept, including studies that have examined the effects of a high-fat diet (HFD) on depressive-like behaviors. Although women are approximately two-fold more likely to develop depressive illness compared to men, most preclinical studies have focused on the effects of HFD in male rodents. Accordingly, the goal of this study was to examine depressive-like behaviors in male and female rats provided access to a HFD. In agreement with prior studies, male and female rats provided a HFD segregate into an obesity phenotype (i.e., diet-induced obesity; DIO) or a diet resistant (DR) phenotype. Upon confirmation of the DR and DIO phenotypes, behavioral assays were performed in control chow, DR, and DIO rats. In the sucrose preference test, male DIO rats exhibited significant decreases in sucrose consumption (i.e., anhedonia) compared to male DR and male control rats. In the forced swim test (FST), male DIO rats exhibited increases in immobility and decreases in climbing behaviors in the pre-test sessions. Interestingly, male DR rats exhibited these same changes in both the pre-test and test sessions of the FST, suggesting that consumption of a HFD, even in the absence of the development of an obesity phenotype, has behavioral consequences. Female rats did not exhibit differences in sucrose preference, but female DIO rats exhibited increases in immobility exclusively in the test session of the FST, behavioral changes that were not affected by the stage of the estrous cycle. Collectively, these studies demonstrate that access to a HFD elicits different behavioral outcomes in male and female rats.

Knowing Our Enemy in the Antimicrobial Resistance Era: Dissecting the Molecular Basis of Bacterial Defense Systems.

Bacteria and their phage adversaries are engaged in an ongoing arms race, resulting in the development of a broad antiphage arsenal and corresponding viral countermeasures. In recent years, the identification and utilization of CRISPR-Cas systems have driven a renewed interest in discovering and characterizing antiphage mechanisms, revealing a richer diversity than initially anticipated. Currently, these defense systems can be categorized based on the bacteria's strategy associated with the infection cycle stage. Thus, bacterial defense systems can degrade the invading genetic material, trigger an abortive infection, or inhibit genome replication. Understanding the molecular mechanisms of processes related to bacterial immunity has significant implications for phage-based therapies and the development of new biotechnological tools. This review aims to comprehensively cover these processes, with a focus on the most recent discoveries.

A discrete-time model of phenotypic evolution

A model is proposed for the phenotypic evolution of a very large population under sustained environmental change and non-overlapping generations, with a single trait considered. Due to an extension of the standard law of quantitative inheritance, each evolutionary mechanism corresponds to a function between random variables associated with distinct stages of the life cycle. Such an approach leads to a two-dimensional map where the dynamics of the phenotypic mean and variance are directly connected. Then, the declining population paradigm is explored in terms of the critical rate of environmental change and using the techniques of the dynamical systems theory. Our results first reveal the opposing pressures on the phenotypic variance due to the conflict between phenotypic load and the ability to pursue the optimum, translated into an optimal value for maximizing the critical rate. Secondly, the introduction of development, through the particular case of linear plasticity, leads to a decreasing degree of stability with the magnitude of plasticity, which means that the recovery time from disturbances is harmed as the plastic effect intensifies, even though no constitutive costs have been assumed, a feature almost as important as the mean fitness to the viability of populations subject to persistent changes. Notwithstanding, the system is stable, and the growth rate benefits from increased plasticity, as expected.

Considering Grouped or Individual Non-Methane Volatile Organic Compound Emissions in Life Cycle Assessment of Composting Using Three Life Cycle Impact Assessment Methods

Composting is a waste management practice that converts organic waste into a product that can be used safely and beneficially as a bio-fertiliser and soil amendment. Non-methane volatile organic compounds (NMVOCs) from composting are known to cause damage to human health and the environment. The impact of waste management on the environment and workers is recognised as a growing environmental and public health concern. Measurements of NMVOCs emitted during composting have been carried out only in a few studies. NMVOC emissions are typically reported as a group rather than as species or speciation profiles. Recognising the need to investigate the issues associated with NMVOCs, the objective of this study is to estimate variation in life cycle assessment (LCA) results when NMVOCs are considered individual emissions compared to grouped emissions and to compare midpoint and endpoint life cycle impact assessment (LCIA) methods. In general, the ReCiPe 2016 LCIA method estimated the highest impact from the composting process in comparison to IMPACT World+ and EF 3.0 for the impact categories of ozone formation, stratospheric ozone depletion, and particulate matter formation. For ReCiPe 2016 and IMPACT World+, the NMVOC emissions were not linked to human toxicity characterisation factors, meaning that the contribution from NMVOC towards human health risks in and around composting facilities could be underestimated. Using individual NMVOCs helps to additionally estimate the impacts of composting on freshwater ecotoxicity and human carcinogenic and non-carcinogenic toxicity potential. If ecotoxicity or toxicity issues are indicated, then LCA should be accompanied by suitable risk assessment measures for the respective life cycle stage.

The PACS-2 protein and trafficking motifs in CCHFV Gn and Gc cytoplasmic tails govern CCHFV assembly

The Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne bunyavirus that causes high mortality in humans with a wide geographic range. This enveloped virus has a tri-segmented negative or ambisense RNA genome that harbors two surface glycoproteins (GP), Gn and Gc. The maturation of the Gn/Gc glycoprotein precursor complex takes place in the ER and is completed through the secretion pathway. Here, we aimed at characterizing the trafficking network exploited by CCHFV GPs during the stages of virus assembly, envelopment, and/or egress. We identified putative membrane trafficking motifs in the cytoplasmic domains (CD) of CCHFV GPs and addressed how they can impact these late stages of the viral life cycle using infection assays, biochemical assays and confocal microscopy in virus-producing cells. Several of the identified CCHFV GP CD motifs could modulate GP transport through the retrograde trafficking network, impacting envelopment and secretion of infectious particles. Finally, we identified the phosphofurin acidic cluster sorting protein 2 (PACS-2) as a crucial host factor that contributes to CCHFV GPs trafficking required for assembly and release of viral particles.