Stable Isotope Tracer Approach Research Articles

The addition of mycoprotein to a mixed-meal impacts postprandial glucose kinetics without altering blood glucose concentrations: a randomised controlled trial

BackgroundMycoprotein is a high-fibre food previously shown to reduce postprandial glucose concentrations when ingested within a mixed-meal. We applied a dual stable isotope tracer approach to determine whether this is due to a reduced rate of appearance of glucose, in participants of ranging BMI.MethodsTwenty-four adults (F = 8, BMI 30 ± 6 kg·m−2) attended 2 trials in a double-blind, randomised, cross-over design. Participants ingested two energy and macronutrient matched milk-based drinks (enriched with 1000 mg [U-13C6] glucose in a subset of 12 participants), containing 50 g glucose and either 0 (CON) or 20 g (MYC) mycoprotein. A primed continuous intravenous infusion of D-[6,6-2H2] glucose determined plasma glucose kinetics over 6 h. Postprandial time-course, and AUC, of glucose and insulin concentration, rate of disappearance (RdT) and appearance of exogenous (RaEx), endogenous (EGP), and total (RaT) plasma glucose were assessed using two- and one-way ANOVA.ResultsDrink ingestion increased blood glucose and serum insulin concentrations (P < 0.05) and were comparable between conditions (P > 0.05). Both RaT and RdT were higher with MYC compared with CON over 6 h (mean 6 h glucose appearance and disappearance increased by 5 and 9%, respectively, P < 0.05). RaEx was not affected by MYC ingestion over 6 h (P > 0.05). The mean contribution of EGP to total glucose appearance was 15% greater with MYC, with a trend towards significance (P = 0.05). There was no relationship between BMI and the response to MYC ingestion for any of the variables (P < 0.05).ConclusionThe ingestion of mycoprotein within a mixed-meal impacted postprandial glucose kinetics, but not blood glucose or serum insulin concentrations, in individuals of ranging BMI.Clinical trial registry number and websiteThis trial was registered at clinicaltrials.gov as NCT04084639 and can be accessed at https://clinicaltrials.gov/ct2/show/NCT04084639.

Compartmental analysis: a new approach to estimate protein breakdown and meal response in health and critical illness.

This study aimed to discuss the use of the pulse stable isotope tracer approach to study changes in metabolism in healthy individuals and critically ill patients. We found that in the postabsorptive state and healthy condition, intracellular protein breakdown and net intracellular protein breakdown, when calculated using the pulse tracer approach, are about double what has previously been reported using the more traditional primed-constant and continuous stable isotope approaches (600 versus 300 grams of protein/day). In critically ill patients, protein breakdown is even higher and calculated to be approximately 900 grams of protein/day, using the pulse tracer approach. Based on these data, we hypothesize that reducing protein breakdown in the postabsorptive state is key when trying to improve the condition of critically ill patients. Moreover, we also used the pulse tracer approach during feeding to better estimate the intracellular metabolic response to feeding. Our first observation is that endogenous protein breakdown does not seem to be reduced during feeding. We also have shown that when consuming a meal with a certain amount of protein, the biological value of that protein meal can be calculated with the pulse tracer approach. In conclusion, using the pulse stable isotope tracer approach to study protein kinetics in the postabsorptive state and during feeding expands our understanding of how dietary proteins can affect human protein metabolism. The intracellular protein synthesis stimulatory effect of a meal is an important factor to consider when calculating the exact protein requirements and needs, particularly in critical illness.

Differential Responses of Nitrate/Ammonium Use to Bicarbonate Supply in Two Brassicaceae Species under Simulated Karst Habitat

In the karst habitats with nitrate-abundant and ammonium-rare soil, the bicarbonate supply plays a crucial role in both inorganic carbon and nitrogen assimilation in various plant species. Consequently, two carbon sources, carbon dioxide (CO2) and bicarbonate (HCO3−), and two nitrogen sources, namely nitrate (NO3−) and ammonium (NH4+) are available for plants. However, variations in the absorption and utilization of nitrate, ammonium, and inorganic carbon during bicarbonate supply in different plants are not well-depicted. In this study, we evaluated the nitrate/ammonium use efficiency and their contributions to the total nitrogen assimilation/utilization capacity at different bicarbonate levels using a bidirectional stable nitrogen isotope tracer approach. The inorganic carbon assimilation, such as the photosynthesis, carbon/nitrogen enzymatic activities, carbon/nitrogen content, nitrogen assimilation/utilization capacity, and nitrate/ammonium contributions to plant growth, were also evaluated to decipher the responses of both carbon and nitrogen metabolism to bicarbonate supply in karst habitats. With the increasing bicarbonate level, Orychophragmus violaceus (Ov) was found to be more available for nitrate to total nitrogen assimilation and utilization than Bn (Brassica napus). Further, it enhanced the contributions of nitrate and nitrogen accumulation/utilization capacity and inorganic carbon assimilation, increasing photosynthesis, carbon/nitrogen enzymatic activities, and carbon accumulation, and promoted the growth in Ov. Though the highest bicarbonate level was conducive to ammonium utilization and water use efficiency in both Ov and Bn, it inhibited total inorganic carbon and nitrogen assimilation, leading to growth suppression in Bn compared to Ov. Moreover, considering the optimistic responses of both carbon and nitrogen assimilation to the high bicarbonate supply in nitrate-abundant, as well as ammonium-rare, environments, we conclude that Ov was more adaptable to the karst habitats. This study provides a novel approach to elucidate the responses of nitrate/ammonium utilization and inorganic carbon assimilation to bicarbonate. Furthermore, the current study reveals the complex interactions among different carbon–nitrogen metabolism pathways in various plants and their adaptations to karst habitats.

Blood 15N:13C Enrichment Ratios Are Proportional to the Ingested Quantity of Protein with the Dual-Tracer Approach for Determining Amino Acid Bioavailability in Humans

ABSTRACTBackgroundAssessment of amino acid bioavailability is of key importance for the evaluation of protein quality; however, measuring ileal digestibility of dietary proteins in humans is challenging. Therefore, a less-invasive dual stable isotope tracer approach was developed.ObjectiveWe aimed to test the assumption that the 15N:13C enrichment ratio in the blood increases proportionally to the quantity ingested by applying different quantities of 15N test protein.MethodsIn a crossover design, 10 healthy adults were given a semi-liquid mixed meal containing 25 g (low protein) or 50 g (high protein) of 15N-labeled milk protein concentrate simultaneous with 0.4 g of highly 13C–enriched spirulina. The meal was distributed over multiple small portions, frequently provided every 20 min during a period of 160 min. For several amino acids, the blood 15N- related to 13C-isotopic enrichment ratio was determined at t = 0, 30, 60, 90, 120, 180, 240, 300, and 360 min and differences between the 2 meals were compared using paired analyses.ResultsNo differences in 13C AUC for each of the measured amino acids in serum was observed when ingesting a low- or high-protein meal, whereas 15N AUC of amino acids was ∼2 times larger on the high-protein meal (P < 0.001). Doubling the intake of 15N-labeled amino acids increased the 15N:13C ratio by a factor of 2.04 ± 0.445 for lysine and a factor between 1.8 and 2.2 for other analyzed amino acids, with only phenylalanine (2.26), methionine (2.48), and tryptophan (3.02) outside this range.ConclusionsThe amino acid 15N:13C enrichment ratio in the peripheral circulation increased proportionally to the quantity of 15N-labeled milk protein ingested, especially for lysine, in healthy adults. However, when using 15N-labeled protein, correction for, e.g., α-carbon 15N atom transamination is advised for determination of bioavailability of individual amino acids. This trial was registered at www.clinicaltrials.gov as NCT02966704.

Does natural feed supply the nutritional needs of shrimp in extensive rice-shrimp ponds? – A stable isotope tracer approach

Rice-shrimp culture systems occur throughout Asia where there are seasonal alterations of fresh and saltwater availability. During the dry season, black tiger shrimp are grown at low densities, and in the wet season, rice, or rice and shrimp are grown together. Previous studies point to issues with suboptimal rice and shrimp production, due, in part, to climatic conditions driving changes in water quality, and pond management practices. However, the availability of natural benthic food supplies for shrimp production is less well studied. This study used a 15N‑nitrogen stable isotope tracer added to replicated enclosures within two rice-shrimp ponds to trace shrimp feeding on benthic natural biota. This experiment was conducted in both the wet and dry seasons. The 15N-ammonium rapidly enriched the 15N signature in the particulate organic matter (POM) in the water column, indicative of significant phytoplankton uptake of nitrogen (N). In contrast there was little enrichment of the benthic algae in the same time frame. After 13 d, most of the shrimp in the replicate enclosures had little or no 15N enrichment. This result occurred across both ponds and in both seasons. This suggests that enriched N was not transferred through the benthic food web to the shrimp. The results of the 15N enclosure experiments were combined with biomass estimates of benthic algae and macrobenthos, shrimp growth data, and linked to other parallel studies of water quality and sediment biogeochemistry in the same ponds. Overall, the findings point to inadequate feed stocks for shrimp, and hence the need to consider supplemental feeding with high quality formulated feeds in order to improve growth rates and survival.

A novel stable isotope tracer method to simultaneously quantify skeletal muscle protein synthesis and breakdown

Background/aimsMethodological challenges have been associated with the dynamic measurement of muscle protein breakdown (MPB), as have the measurement of both muscle protein synthesis (MPS) and MPB within the same experiment. Our aim was to use the transmethylation properties of methionine as proof-of-concept to measure rates of MPB via its methylation of histidine within skeletal muscle myofibrillar proteins, whilst simultaneously utilising methionine incorporation into bound protein to measure MPS. ResultsDuring the synthesis measurement period, incorporation of methyl[D3]-13C-methionine into cellular protein in C2C12 myotubes was observed (representative of MPS), alongside an increase in the appearance of methyl[D3]-methylhistidine into the media following methylation of histidine (representative of MPB). For further validation of this approach, fractional synthetic rates (FSR) of muscle protein were increased following treatment of the cells with the anabolic factors insulin-like growth factor-1 (IGF-1) and insulin, while dexamethasone expectedly reduced MPS. Conversely, rates of MPB were reduced with IGF-1 and insulin treatments, whereas dexamethasone accelerated MPB. ConclusionsThis is a novel stable isotope tracer approach that permits the dual assessment of muscle cellular protein synthesis and breakdown rates, through the provision of a single methionine amino acid tracer that could be utilised in a wide range of biological settings.

Non-proteolytic ubiquitination of Hexokinase 2 by HectH9 controls tumor metabolism and cancer stem cell expansion

Enormous efforts have been made to target metabolic dependencies of cancer cells for developing new therapies. However, the therapeutic efficacy of glycolysis inhibitors is limited due to their inability to elicit cell death. Hexokinase 2 (HK2), via its mitochondrial localization, functions as a central nexus integrating glycolysis activation and apoptosis resilience. Here we identify that K63-linked ubiquitination by HectH9 regulates the mitochondrial localization and function of HK2. Through stable isotope tracer approach and functional metabolic analyses, we show that HectH9 deficiency impedes tumor glucose metabolism and growth by HK2 inhibition. The HectH9/HK2 pathway regulates cancer stem cell (CSC) expansion and CSC-associated chemoresistance. Histological analyses show that HectH9 expression is upregulated and correlated with disease progression in prostate cancer. This work uncovers that HectH9 is a novel regulator of HK2 and cancer metabolism. Targeting HectH9 represents an effective strategy to achieve long-term tumor remission by concomitantly disrupting glycolysis and inducing apoptosis.

Calcium Absorption during Fruit Development in ‘Honeycrisp’ Apple Measured Using 44Ca as a Stable Isotope Tracer

Calcium (Ca) sprays are commonly used to control Ca-related disorders such as bitter pit in apple. Increases in the frequency and the amount of Ca applied directly to the fruit have increased fruit Ca levels and are associated with a reduction in bitter pit incidence. However, the absorption efficiency at different fruit developmental stages is poorly understood. Here, the absorption efficiency was measured using 44Ca stable isotope applied to 30 individual fruit at five different times every 2 weeks between June drop and 2 weeks before harvest in a medium-density ‘Honeycrisp’ orchard. Fruit size, spray adhesion, and Ca and potassium (K) content were monitored weekly for 12 weeks between 26 May and 13 Aug. 2015. At harvest, the 44Ca-labeled fruit was picked and separated into peel and inner fruit for mass balance analysis of 44Ca absorption to regions of the fruit that are important to prevent Ca-related disorders. As expected, δ44Ca was greater in the peel than the interior of the fruit. However, there was a significant amount of 44Ca present in the inner fruit at harvest for all five applications applied during the growing season. Using a stable isotope tracer approach, we present evidence that Ca is absorbed throughout fruit development. These findings support current recommendations for frequent Ca applications in low concentrations throughout fruit development to increase fruit Ca levels and reduce the incidence of bitter pit in ‘Honeycrisp’ apple.

Dual-tracer-based isotope turnover rates in a highly invasive mysid Limnomysis benedeni from Lake Constance.

Understanding the ecological patterns of invasive species and their habitats require an understanding of the species’ foraging ecology. Stable carbon (δ13C) and nitrogen (δ15N) isotope values provide useful information into the study of animal ecology and evolution, since the isotope ratios of consumers reflect consumer's dietary patterns. Nevertheless, the lack of species‐ and element‐specific laboratory‐derived turnover rates could limit their application. Using a laboratory‐based dual stable isotope tracer approach (Na15 NO 3 and NaH13 CO 3), we evaluated the δ15N and δ13C isotope turnover rates in full‐grown adult invasive Limnomysis benedeni from Lake Constance. We provide δ15N and δ13C turnover rates based on nonlinear least‐squares regression and posterior linear regression models. Model precisions and fit were evaluated using Akaike's information criterion. Within a couple of days, the δ15N and δ13C of mysids began to change. Nevertheless, after about 14 days, L. benedeni did not reach equilibrium with their new isotope values. Since the experiment was conducted on adult subjects, it is evident that turnover was mainly influenced by metabolism (in contrast to growth). Unlike traditional dietary shifts, our laboratory‐based dual stable isotope tracer approach does not shift the experimental organisms into a new diet and avoids dietary effects on isotope values. Results confirm the application of isotopic tracers to label mysid subpopulations and could be used to reflect assimilation and turnover from the labeled dietary sources. Field‐based stable isotope studies often use isotopic mixing models commonly assuming diet‐tissue steady state. Unfortunately, in cases where the isotopic composition of the animal is not in equilibrium with its diet, this can lead to highly misleading conclusions. Thus, our laboratory‐based isotopic incorporation rates assist interpretation of the isotopic values from the field and provide a foundation for future research into using isotopic tracers to investigate invasion ecology.

Tracing Consumer-Derived Nitrogen in Riverine Food Webs

The flux of consumer-derived nutrients is recognized as an important ecosystem process, yet few studies have quantified the impact of these fluxes on freshwater ecosystems. The high abundance of bivalves in both marine and freshwater suggests that bivalves can exert large effects on aquatic food webs. The objective of our study was to determine the importance of unionid mussel-derived nitrogen (MDN) to the food web. We used a stable isotope tracer approach in conjunction with nutrient uptake and excretion experiments. We fed mussels (Lampsilis siliquiodea, n = 249) a 15N-enriched algal diet and placed them into a N-limited stream for 63 days. Mussel hemolymph was non-lethally sampled over the course of the experiment to measure tissue turnover of δ15N and excretion experiments were done to model the amount of N mussels provided in comparison to stream N uptake demand. Multiple food web pools were sampled twice prior and five times following the mussel addition to trace the 15N through the food web. Our mussel excretion rates in comparison to areal uptake demand suggested that mussel excretion can account for 40% of the total N demand in this stream. Our enrichment showed that MDN was entering the food web and supplied up to 19% of the N in specific compartments of the food web near the mussel bed. When scaled to a natural mussel aggregation, our results suggest up to 74% of N in the food web may be mussel-derived. Our results show that N supplied by mussels can be an important nutrient subsidy that provides food web support.

Use of [13C18] Oleic Acid and Mass Isotopomer Distribution Analysis to Study Synthesis of Plasma Triglycerides In Vivo: Analytical and Experimental Considerations

We have previously reported on a liquid chromatography-mass spectrometry method to determine the disposition of [(13)C18]-oleic acid following intravenous and oral administration in vivo. This approach has enabled us to study a variety of aspects of lipid metabolism including a quantitative assessment of triglyceride synthesis. Here we present a more rigorous evaluation of the constraints imposed upon the analytical method in order to generate accurate data using this stable-isotope tracer approach along with more detail on relevant analytical figures of merit including limits of quantitation, precision, and accuracy. The use of mass isotopomer distribution analysis (MIDA) to quantify plasma triglyceride synthesis is specifically highlighted, and a re-evaluation of the underlying mathematics has enabled us to present a simplified series of equations. The derivation of this MIDA model and the significance of all underlying assumptions are explored in detail, and examples are given of how it can successfully be applied to detect differences in plasma triglyceride synthesis in lean and high-fat diet fed mouse models. More work is necessary to evaluate the applicability of this approach to triglyceride stores with slower rates of turnover such as in adipose or muscle tissue; however, the present report provides investigators with the tools necessary to conduct such studies.

A dual stable isotope tracer method for the measurement of surfactant disaturated-phosphatidylcholine net synthesis in infants with congenital diaphragmatic hernia.

The aim of the study was to measure for the first time in humans surfactant disaturated-phosphatidylcholine (DSPC) net synthesis and kinetics by using a novel, dual stable isotope tracer approach. Ten infants with congenital diaphragmatic hernia [CDH; birth weight, 3.4 +/- 0.2; gestational age, 39.8 +/- 0.4 wk] and 6 age-matched control subjects with no lung disease (birth weight, 3.2 +/- 0.3 kg; gestational age, 39.1 +/- 1.1 wk), all of whom were admitted to the neonatal intensive care unit (Padua, Italy), were studied. All infants received simultaneously an intratracheal (carbon-13 di-palmitoyl-phosphatidylcholine) and an i.v. (deuterated palmitic acid) stable isotope tracer. Isotopic enrichment curves of DSPC from sequential tracheal aspirates were analyzed by mass spectrometry. DSPC kinetic data were expressed as mean +/- SEM and compared by the Mann-Whitney test. DSPC net synthesis from plasma palmitate was nearly identical in infants with CDH and control subjects (8.6 +/- 2.2 and 8.1 +/- 1.5 mg. kg(-1). d(-1); P = 0.7). DSPC apparent pool size was 36.7 +/- 7.5 and 58.5 +/- 9.1 mg/kg (P = 0.07) and half-life was 26.7 +/- 4.5 and 50.3 +/- 9.7 h (P = 0.03) in infants with CDH and control subjects, respectively. Both DSPC turnover and percentage of catabolism/recycling significantly correlated with duration of mechanical ventilation. In conclusion, the measurements of net DSPC synthesis and catabolism/recycling were reported for the first time in humans. Mean net DSPC synthesis was approximately 8 mg. kg(-1). d(-1). No significant differences were found between control subjects and infants with CDH. DSPC turnover was faster in infants with CDH, presumably reflecting an increased DSPC catabolism/recycling. Whether this may ultimately lead to a secondary surfactant deficiency in infants with CDH is still to be ascertained.

Microbial utilization of estuarine dissolved organic carbon: a stable isotope tracer approach tested by mass balance

The natural stable isotope values of different plants have been used to trace the fate of organic carbon that enters estuarine ecosystems. Experiments were designed to determine the magnitude of (delta) (sup13)C changes of dissolved organic carbon (DOC) derived from tidal marsh vegetation that occurred during bacterial decomposition. Bacteria were grown on DOC leached from estuarine Spartina alterniflora and Typhus angustifolia plants. In four experiments, 25 to 80% of the initial carbon (2.6 to 9.1 mM organic C) was converted to bacterial biomass and CO(inf2). Mass balance calculations showed good recovery of total C and (sup13)C at the end of these experiments (100% (plusmn) 14% total C; (plusmn) 1(permil) (delta) (sup13)C). The (delta) (sup13)C values of DOC, bacterial biomass, and respired CO(inf2) changed only slightly in the four experiments by average values of -0.6, +1.4, and +0.5(permil), respectively. These changes are small relative to the range of (delta) (sup13)C values represented by different organic carbon sources to estuaries. Thus, microbial (delta) (sup13)C values determined in the field helped to identify the source of the carbon assimilated by bacteria.

Development of the stable isotope tracer approach for studies of copper turnover in the rat and mouse

The stable isotope tracer approach was explored for long-term investigations of copper turnover in the adult rat and mouse, with inductively coupled plasma mass spectrometry for isotope measurements. The isotopic measurement method permitted precision and accuracy of <1.0%, with an overall sample blank of <0.05 μg copper. Rats were fed a copper-deficient diet and deionized water with (+Cu) or without (−Cu) copper (20 μg/ml). Both groups underwent a single-day replacement of drinking water with 20 μg/ml of 65Cu. Compared with the baseline isotope ratio ( 65Cu/ 63Cu) of 0.462 ± 0.002, blood plasma ratios for the +Cu group on days 2, 7, and 14 postdosing were 0.702 ± 0.021, 0.557 ± 0.004, and 0.474 ± 0.001, respectively. The corresponding data for liver were 0.652 ± 0.018, 0.560 ± 0.005, and 0.482 ± 0.001, respectively. For the −Cu group, respective plasma ratios were 1.580 ± 0.04, 0.917 ± 0.02, and 0.664 ± 0.01 for days 2, 7, and 14 postdosing, and the ratios for liver were 0.978 ± 0.02, 0.876 ± 0.04, and 0.739 ± 0.03. Mice previously made copper deficient to varying degrees were given a single-day replacement with the label. When the 24-hour postdosing isotope ratios in the livers of these mice were correlated with the activity of plasma ceruloplasmin, a negative correlation ( r = −0.85) was observed. Isotope enrichment in both rats and mice was greater in the copper-deficient animals compared with the controls.

Distribution of ceruloplasmin- ceruloplasmin-bound 67 Cu in the rat.

ARTICLESDistribution of ceruloplasmin- ceruloplasmin-bound 67 Cu in the ratN Marceau, and N AspinN MarceauSearch for more papers by this author, and N AspinSearch for more papers by this authorPublished Online:01 Jan 1972https://doi.org/10.1152/ajplegacy.1972.222.1.106MoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByIntravenous and oral copper kinetics, biodistribution and dosimetry in healthy humans studied by [64Cu]copper PET/CT18 June 2020 | EJNMMI Radiopharmacy and Chemistry, Vol. 5, No. 1Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 days of estivation in air14 September 2011 | Journal of Comparative Physiology B, Vol. 182, No. 2Clinical and Genetic Association of Serum Ceruloplasmin With Cardiovascular RiskArteriosclerosis, Thrombosis, and Vascular Biology, Vol. 32, No. 2ReferencesCeruloplasmin: The Copper Transport Protein With Essential Oxidase Activity22 November 2006Copper metabolism in analbuminaemic rats fed a high-copper dietComparative Biochemistry and Physiology Part A: Physiology, Vol. 110, No. 3The metabolism of copper during pregnancy —a reviewFood Chemistry, Vol. 54, No. 1Biological Efficacy of Copper in Chicken BileThe Journal of Nutrition, Vol. 123, No. 5The transport of iron and copper across the cell membrane: different mechanisms for different metals?28 February 2007 | Proceedings of the Nutrition Society, Vol. 51, No. 2Uptake of 67Cu by isolated human trophoblast cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1135, No. 2The Accumulation of Copper by Microvillar Vesicles Isolated from Human Placenta ,The Journal of Nutrition, Vol. 122, No. 6Simulation of copper metabolism by mammalsComputers in Biology and Medicine, Vol. 22, No. 1-2Copper Uptake and Retention in Liver Parenchymal Cells Isolated from Nutritionally Copper-Deficient RatsThe Journal of Nutrition, Vol. 121, No. 8Effect of food restriction on tissue carnitine concentration in ratsClinical Nutrition, Vol. 10, No. 2Experimental carnitine depletion in ratsClinical Nutrition, Vol. 10, No. 2Mechanisms of Metal Transport Across Liver Cell Plasma Membranes22 September 2008 | Drug Metabolism Reviews, Vol. 23, No. 1-2Development of the stable isotope tracer approach for studies of copper turnover in the rat and mouseThe Journal of Nutritional Biochemistry, Vol. 1, No. 5Host Defenses against Copper ToxicityCopper-binding protein in acute copper poisoningForensic Science International, Vol. 38, No. 1-2Albumin has no role in the uptake of copper by human fibroblastsJournal of Inorganic Biochemistry, Vol. 31, No. 2Role of Ceruloplasmin in Copper TransportThe distribution of zinc and copper in plasma, erythrocytes and erythrocyte membranes of rainbow trout (Salmo Gairdneri)Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, Vol. 87, No. 2The role of liver endothelium in the binding and uptake of ceruloplasmin: Studies with colloidal gold probeJournal of Ultrastructure Research, Vol. 90, No. 2Copper and InflammationIn vitro assessment of the toxicity of metal compoundsBiological Trace Element Research, Vol. 6, No. 2Copper HomeostasisCopperCaeruloplasmin: Physiological and Pathological Perspectives27 September 2008 | CRC Critical Reviews in Clinical Laboratory Sciences, Vol. 14, No. 45 Anti-Rheumatic Drugs: Present Deadlock and New VistasMetabolic model and dosimetric data for copperBulletin of Mathematical Biology, Vol. 40, No. 2Metabolic Disorders of Copper MetabolismMetabolism of labeled carnitine in the ratArchives of Biochemistry and Biophysics, Vol. 175, No. 1The Biological Role of Ceruloplasmin and Its Oxidase ActivityEvidence for ceruloplasmin as a copper transport proteinBiochemical and Biophysical Research Communications, Vol. 67, No. 4Copper Content and Exchange in Mammalian HeartsCirculation Research, Vol. 35, No. 1The intracellular distribution of the radiocopper derived from ceruloplasmin and from albuminBiochimica et Biophysica Acta (BBA) - Protein Structure, Vol. 328, No. 2The association of the copper derived from ceruloplasmin with cytocupreinBiochimica et Biophysica Acta (BBA) - Protein Structure, Vol. 328, No. 2 More from this issue > Volume 222Issue 1January 1972Pages 106-110 Copyright & PermissionsCopyright © 1972 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1972.222.1.106PubMed5060209History Published online 1 January 1972 Published in print 1 January 1972 Metrics