Background: This study aimed to determine the validity and reliability of the countermovement jump (CMJ) as a dynamic eccentric (Ecc) strength test. Methods: Thirty-three college male student-athletes were recruited to participate in this study. The participants first performed CMJs with the second consisting of one repetition maximum back squat (1RM-BS) test. CMJ and 1RM-BS tests were performed on twin force plates. Results: The CMJ had significant correlations with the Ecc peak force (EccPF), and Ecc mean force (EccMF) of 1RM-BS, respectively (r = 0.61−0.69). Moreover, all parameters had a coefficient of variation (CV) < 10%. The intraclass correlation coefficient (ICC) values were moderate to excellent for each metric using the CMJ (0.94−0.97). The 1RM-BS and CMJ EccPF, EccMF Bland-Altman bias estimate variance ratio is 1.31−1.67, showing a moderate-large correlation in the Bland-Altman plot. Conclusions: CMJ ECC phase kinetics were associated with the 1RM-BS EccPF and EccMF. The CMJ can be an alternative tool for eccentric dynamic strength assessment.