Germination Length Research Articles

Photoselective coverings influence plant growth, root development, and buddability of citrus plants in protected nursery

Use of protective coverings can be an economically viable option in fruit nurseries than in fully mature commercial orchards. In protected nurseries, photoselective coverings can influence the growth of rootstocks, budding success, and budding growth of the budded scion cultivar. The effect of photoselective coverings (white, silver, green, red and stainless steel net house) along with open conditions (control) was studied on plant growth, nutrient uptake and root growth of rough lemon and Carrizo citrange rootstock seedlings which were subsequently budded with Kinnow and Daisy mandarin cultivar buds, respectively. The rough lemon plants under red net had better height, internodal length and budding success, while in open conditions, higher seedling diameter, root diameter, shoot and root dry weight, leaf number and secondary root number were recorded in rough lemon seedlings. The Carrizo seedlings in red net had better seedling diameter, internodal length, leaf area and number, shoot dry weight and budding success. The higher nitrogen, phosphorus, potassium and zinc levels were recorded in the leaves of rough lemon and Carrizo citrange under red shade net. The plant height of rough lemon seedlings was positively correlated to internodal length, budding success and leaf areaas well as leaf phosphorus and iron levels. The highest buddable seedlings were recorded in open conditions but, budding success in red shade net house. The highest sprout lengths of Kinnow and Daisy mandarin saplings were recorded in red net followed by green net and white net. Higher photosynthesis and stomatal conductance were recorded under red shade net, whereas lowest PAR interception was recorded in open conditions.

Effect of slightly acidic electrolyzed water on natural Enterobacteriaceae reduction and seed germination in the production of alfalfa sprouts

Microbial contamination of sprouts commonly occurs because of the pathogens present on and in the seeds and the optimal conditions for bacteria growth provided during the germination and sprouting processes. This study examined the decontamination effect of slightly acidic electrolyzed water (SAEW), a ‘generally recognized as safe’ (GRAS) disinfectant, in the production process of alfalfa sprouts. SAEW with various available chlorine concentrations (ACC, 25, 35, 45 mg/L) and different pH levels (5.0, 5.7 and 6.4) was used to soak seeds for different length of time (0.5 and 6 h), after which the variations in natural Enterobacteriaceae, water absorption and seed germination (germination rate, weight and length of sprouts) were determined. The results showed that when the seeds were soaked with SAEW, albeit with different ACC (25, 35 and 45 mg/L) and pH levels (5.0, 5.7 and 6.4), a significant reduction of Enterobacteriaceae and no negative effect on sprout quality was observed. The water absorption and germination rates were also not significantly adversely affected by SAEW soaking. These findings suggest that SAEW could be used to decontaminate natural Enterobacteriaceae in the production of alfalfa sprouts, with no negative side effects on the alfalfa seeds.

Physiological basis of propagation studies in commercially important difficult to root ornamental shrub <em>Thuja orientais</em>

Among different types of cutting, tip cuttings showed early sprouting, highest number of sprouts per cutting per cutting and length of longest sprout. Subsequently, highest number of primary roots per cutting, secondary roots and length of longest root were found with same type of cutting. Among different levels of IBA, IBA at 4000 ppm resulted in early sprouting, maximum number of sprouts per cutting per cutting and length of longest sprout. Subsequently, highest number of primary roots per cutting, secondary roots and length of longest root were found with same treatment. Among two different media used soil + sand + cocopeat + VAM media recorded early sprouting, maximum number of sprouts per cutting per cutting and length of longest sprout. Subsequently, highest number of primary roots per cutting, secondary roots and length of longest root were found with same treatment.

Influence of iba and phb on regeneration of Kagzi lime (<em>Citrus aurantifolia </em>Swingle) through stem cutting

Kagzi lime propagated through hardwood cuttings with low percentage of rooting. Investigations on influence of IBA and PHB on regeneration of cuttings were carried out during the year 2017-18 at Horticulture Garden, College of Horticulture, C.S. Azad University of Agriculture and Technology, Kanpur. Minimum days to sprouting (20.64), maximum number of sprouts per cutting (5.88), maximum percentage of cuttings sprouted (17.68%), maximum length of sprouts (14.88 cm), maximum diameter of sprouts (3.28 cm), maximum number of roots per cutting (42.02), longest root (2.24 cm), diameter of root (6.24 cm), percentage of rooted cuttings (70.12%) and maximum survival percentage (82.66%) were recorded with treatment IBA 2500ppm + PHB 1000ppm (T9). The treatment with IBA 2000ppm + PHB 1000ppm (T8) recorded maximum number of leaves (21.02) and maximum leaf length (6.38 cm). The maximum days to sprouting (32.00), minimum number of sprouts per cutting (3.64), minimum sprouting percentage (11.28%), minimum (7.85 cm) length of sprout per cutting, minimum diameter of sprouts (1.98 cm), minimum number of leaves per cutting (14.40), minimum length of leaves (4.10 cm), minimum (3.20 cm) width of leaves, minimum number of roots per cutting (29.80), minimum length of root (1.10 cm), minimum diameter of root (2.57 cm), minimum (48.02%) rooted cuttings and lowest survival percentage (48.26%) were noted under control (T0).

Concentrations of cytokinin and the use of agricultural residues in the in vitro propagation media of highbush blueberry

ABSTRACT: The objective of the research was to evaluate cytokinin concentrations and agricultural residues as physical structuring agents of the culture medium in the in vitro propagation of highbush blueberry (Vaccinium corymbosum ‘Duke’). To that end, three different concentrations of 2-isopentenyladenine (2iP) (0, 5, and 10 mg L-1)in woody plant medium (WPM) and five structuring agents (in natura rice husks, carbonized rice husks, coconut fiber, S-10 Beifort®, and agar)were tested. After 60days of culture, the following parameters were evaluated: survival (%),sprout and shoot lengths (cm),propagation rate, number of sprouts, and shoot dry mass (g). The substrates used as structuring agents were analyzed in terms of pH and electrical conductivity. The in natura rice husk, S-10 Beifort®, and carbonized rice husk did not differ from the agar in terms of the sprout length. The shoots were longer (1.64 cm) in cultures within natura rice husk than in other treatments. In the absence of 2iP, S-10 Beifort® resulted in the highest propagation rate (2.97). Concerning the number of sprouts, S-10 Beifort®, in natura rice husks, and carbonized rice husks did not differ from agar. However, when the regulator was added, the agar performed better in comparison to the other residues. Thus, in natura rice husks, carbonized rice husks, and S-10 Beifort®are potential physical structuring agents of the culture medium that can be used without 2iP.

Germination conditions influence the physical characteristics, isoflavones, and vitamin C of soybean sprouts

Abstract: The objective of this work was to evaluate the effects of the germination conditions of 'BRS 216' soybean (Glycine max) on the length, yield, and isoflavone contents of sprouts. A 23 factorial arrangement was used to evaluate the variables soaking time, irrigation frequency, and germination time. Sprouts that showed better length and yield and higher isoflavone contents were evaluated for their chemical composition and vitamin C content. Soaking and germination time of soybean showed a positive and significant linear effect on sprout length. However, only germination time showed a significant positive linear effect on yield, and a significant negative linear effect on the content of malonylglycosides, aglycones, and total isoflavones. Soybean germination conditions were established at 6 hours of soaking, three days of germination, and 8 hours of irrigation frequency. Under these conditions, the obtained sprouts show a more preserved chemical composition, besides higher contents of β-glycosides, malonylglycosides, aglycones, total isoflavones, and vitamin C than the nongerminated soybean. Under suitable germination conditions, it is possible to produce soybean sprouts with better physical characteristics, higher yield, and higher contents of isoflavones, aglycones, and vitamin C.

Estimation of salt tolerance of tritical vrieties inrtificialy conditions

This article investigated the statistical differences between germination and development of 11 triticale varieties at different concentrations of NaCl salt (0.5%, 1.0%, 1.5%). Fifteen days later, the laboratory productivity of the samples, germination length, and root length were measured. Analyzing the process of root formation of plants in different saline conditions in the varieties used in the study, it was observed that the formation and development of seeds in artificially saline conditions slowed down under high concentration saline conditions, i.e. at a concentration of 1.5% NaCl salt Sardor, to the root system of GulDU and Valintin-90 varieties was more developed than other specimens. Decrease in field productivity depends on climatic conditions and other factors. It should be noted that the more favorable the germination conditions, the shorter the germination time and the higher the field yield. Our experiment encompasses the features and characteristics, creating different saline resistance and their changes depending on salinity variation under the laboratory conditions. Seed germination is directly dependent on the productivity under the laboratory conditions. On the basis of these data, it can be said that during the evaluation and selection of triticale specimens, it is important to take into account the condition of grasses and root systems in saline conditions.

Prospects for cultivation Triticum aestivum L. and Secale cereale L. on the barren substrates

Studies on the influence of polymer hydrogels from different manufacturers on the morphometric parameters of microalgae cells of Chlorella vulgaris, Eustigmatos magnus and Scotiellopsis sp. and the possibility of their joint use in sowing seeds of Triticum aestivum and Secale cereale in barren substrates are presented. The optimal effects of a particular algae crop in combination with a particular hydrogel and their dependence on the soil type with a joint effect on the seeds of cereal crops were revealed. The germination ability analysis of higher plants seeds of two studied species showed the effectiveness of “BSPU” hydrogel with E. magnus culture liquid when sown on sand. Indicators of the length of sprouts and roots of cereals seeds allowed us to build a number of effective effects of microalgae: E. magnus > S. sp. > Ch. vulgaris. The results of the study revealed the individuality of the crop in combination: substrate/hydrogel/microalgae, which must be taken into account for the possibility of their effective joint use in reclamation processes.

Cocoa shell substrate, indolebutyric acid doses and length of cuttings in the rooting of Fridericia chica

ABSTRACT Fridericia chica (Bonpl.) L. G. Lohmann has medicinal properties and potential for commercial cultivation; however, the propagation characteristics of this species have been poorly evaluated. This study aimed to analyze the length of cuttings, indolebutyric acid (IBA) doses and substrates containing different proportions of cocoa shell in F. chica cuttings. Two experiments were carried out. In the first one, the treatments were basal cuttings of 10 cm and 20 cm in length and four IBA concentrations (0 mg L-1, 2,000 mg L-1, 4,000 mg L-1 and 6,000 mg L-1). In the second experiment, substrates obtained from five proportions of cocoa shell (CS) and expanded vermiculite (EV) compost were tested: 100 % CS; 100 % EV; 50 % CS + 50% EV; 30 % CS + 70 % EV; 70 % CS + 30 % EV. The rooting percentage, sprouting height and dry biomass production were evaluated. The production of F. chica seedlings can be performed by basal cuttings with 20 cm in length, which allow achieving a rooting rate of 91 %. The ideal IBA dose to obtain a maximum sprout length is 3,600 mg L-1. Mixtures composed of 50 % CS + 50 % EV and 30 % CS + 70 % EV were the best substrates for the production of F. chica seedlings.

발아귀리의 싹 길이별 항산화 활성 및 기능성분

본 연구는 국내에서 육종된 쌀귀리 품종 중 하나인 대양을 시료로 하였다. 발아시킨 후 싹 길이별로 얻은 발아귀리의 일반성분, 총 식이섬유, 색도와 총 폴리페놀 및 총 플라보노이드, 총 베타글루칸, 아베난쓰라마이드 함량을 분석하였고, 항산화 활성을 알아보기 위해 DPPH 라디칼, ABTS 라디칼소거 활성을 측정하였다. 색도 측정 결과는 L값(71.71~73.42) 및 b값(14.03~15.57)이 싹 길이가 증가할수록 높아지는 경향이었다. 총 폴리페놀 및 총 플라보노이드 함량은 싹 길이 1 mm 처리구가 가장 높게 나타났고, 발아가 진행되면서 함량이 증가하다가 싹 길이 5 mm부터 감소하는 경향을 나타냈다. 싹 길이 1 mm, 5 mm 처리구에서 DPPH 라디칼과 ABTS 라디칼 소거 활성이 우수한 것으로 나타났고, 발아가 더 진행되면서 감소하는 경향이었다. 총 베타글루칸함량의 경우 싹 길이 1 mm(4.83%)까지는 발아가 진행되면서 베타글루칸 함량이 높게 나타났으나 싹 5 mm(4.28%)부터는 감소하는 경향이었다. 아베난쓰라마이드 중에서 아베난쓰라마이드 C는 싹 길이 1 mm가 158.63 μg/g으로 함량이 가장 높았으며, 싹 길이 10 mm 이상 처리구는 126.49μg/g으로 가장 낮았다. 전반적으로 발아한 귀리가 발아 전 귀리보다 항산화 활성 및 항산화 성분 함량이 증가하는 것으로 나타났으며, 발아 시 적당한 싹 길이를 포함한 귀리를 식품산업 소재 및 기초연구자료로 널리 이용될 수 있을 것으로 생각된다.

Effects of Chitting Duration on Early Maturation of Potatoes in a Short Season Environment

The objective of this study was to assess the efficacy of pre-plant light-sprouting (chitting) on hastening the maturation of potato. Four cultivars with different maturities (‘Superior’, ‘Haryeong’, ‘Seohong’, and ‘Jayoung’) were used over three different growing seasons from 2016 to 2018. Different degrees of physiological age were obtained by exposing the seed lots of each cultivar to sprout-inducing conditions (13–15 °C temperature and 40–50% relative humidity (RH) in 11–20 μmol m−2 s−1 of diffuse light), for 0, 2–4, 6–7, and 10–11 weeks before planting. As the sprout-inducing periods increased, seeds physiologically aged, as evidenced by the increase in the percent sprouted eyes and sprout length per tuber, and the resulting stem numbers per plant. Moreover, in a longer the chitting duration, the increase in the number of stems was highly correlated with the tendency to have more tubers per plant. Physiologically aged seed significantly hastened plant growth through the decreased number of days to emergence, tuberization, and canopy development for all cultivars regardless of the growing season. The accelerated growth of shoots and tubers resulted in an increase in the harvest index, yield, and specific gravity of tubers, with no effect on second growth. However, there were practically no consistent benefits in the early-maturing cultivar Superior. That made it possible for the crop, especially medium- and late-maturing cultivars, to be harvested earlier in the short growing season environment. The magnitude of light-sprouting-induced effects depended on the chitting duration regimes, the maturities of cultivars, and the growing season environments.

Immobilized RGD concentration and proteolytic degradation synergistically enhance vascular sprouting within hydrogel scaffolds of varying modulus

Insufficient vascularization limits the volume and complexity of engineered tissue. The formation of new blood vessels (neovascularization) is regulated by a complex interplay of cellular interactions with biochemical and biophysical signals provided by the extracellular matrix (ECM) necessitating the development of biomaterial approaches that enable systematic modulation in matrix properties. To address this need poly(ethylene) glycol-based hydrogel scaffolds were engineered with a range of decoupled and combined variations in integrin-binding peptide (RGD) ligand concentration, elastic modulus and proteolytic degradation rate using free-radical polymerization chemistry. The modularity of this system enabled a full factorial experimental design to simultaneously investigate the individual and interaction effects of these matrix cues on vascular sprout formation in 3 D culture. Enhancements in scaffold proteolytic degradation rate promoted significant increases in vascular sprout length and junction number while increases in modulus significantly and negatively impacted vascular sprouting. We also observed that individual variations in immobilized RGD concentration did not significantly impact 3 D vascular sprouting. Our findings revealed a previously unidentified and optimized combination whereby increases in both immobilized RGD concentration and proteolytic degradation rate resulted in significant and synergistic enhancements in 3 D vascular spouting. The above-mentioned findings would have been challenging to uncover using one-factor-at-time experimental analyses.

In vivo removal of profenofos in agricultural soil and plant growth promoting activity on Vigna radiata by efficient bacterial formulation

This study evaluated the plant growth and profenofos (PF) removal efficiency of Acinetobacter sp.33F and Comamonas sp. 51 F bacteria as individual strains and in combination F1. Plant growth-promoting activities such as indole 3 acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, phosphate solubilization, ammonia production, and exopolysaccharide (EPS) production were observed in Acinetobacter sp. 33 F and Comamonas sp. 51 F. However, PGP properties observed were higher in Acinetobacter sp. 33 F as compared to the Comamonas sp. 51 F. In pot sand and pot soil studies, the physiological parameters such as sprout length, shoot length, root length, chlorophyll a, chlorophyll b, and carotenoids were higher for combination F1. PF degradation in pot sand and pot soil resulted in highest degradation by combination F1. In pot soil study, soil enzyme activities such as cellulase, dehydrogenase, urease, protease, and phosphate activities and root cross-section area, total stele area and xylem vessel area were recorded higher for the formulation F1. The study demonstrated that the together Acinetobacter sp. 33 F and Comamonas sp. 51 F as formulation has higher plant growth-promoting activities as compared to the individual bacteria.

Effects of biochar and peat on salt-affected soil extract solution and wheat seedling germination in the Yellow River Delta

Salt-affected soils are widely distributed in arable croplands, so it is important to reclaim these soils. In this study, the effects of different biochar/peat doses (1, 3, and 5%) on 1:5 salt-affected soil: water extract solutions with different soil salinity levels after seven days shaking; and on winter wheat seedling growth after 80 hours are discussed. Results showed that the SAR and Cl-/SO42- ratio varied because of the changes of ion composition in the soil extract solution caused by the addition of biochar and peat. The maximum length of root and sprout of wheat grown in S1 (very slightly saline) soil extract treated by biochar/peat were 8.0/7.14 and 4.86/4.50 cm, respectively. The average length of wheat root and sprout grown in S2 (moderately saline) soil extract treated by peat was higher than that in soil treated by biochar. The results indicated that biochar and peat modified the ion composition of salt-affected soil extract solutions, as well as that of wheat. The abundant beneficial ions K+ or Ca2+ in biochar/peat might be beneficial to reclaim the salt-affected soil, and the exogenous ions altered the composition of the soil solution to promote the seedling growth or enhance the resistance of plants to the salt stress. Moreover, cation exchange took place among soil, biochar/peat, and soil solution. The proportion of harmful Na+ declined (SAR decreased) in the soil solution, which could be beneficial for the reclamation of the salt-affected soils.

ASOCIACIÓN ENTRE CULTIVOS DE CACAO (Theobroma cacao L.) Y VAINILLA (Vanilla planifolia Jacks. ex Andrews) EN UN SISTEMA AGROFORESTAL EN COMALCALCO, TABASCO

<p>Cocoa (<em>Theobroma cacao</em> L.) and vanilla (<em>Vanilla planifolia</em> Jacks. ex Andrews) are considered vegetal crops with great economical, social and cultural importance for the families of mexican tropics. However, in recent years the production of these crops has considerably decreased. Faced with this situation, determining the feasibility of combined cultivation of both species may represent an alternative to improve farmers profitability. Through this work, farming alternatives are proposed with different treatments such as cocoa and vanilla. We evaluated 60 cocoa plants and 80 vanilla cuttings, divided into five treatments: cocoa and vanilla with no-interaction (Tc), vanilla on cocoa” (Vp/Tc), cocoa and vanilla using an inert tutor at a distance of one meter (Tc-Vp1m), cocoa and vanilla using an inert tutor at a distance of two meters “(Tc-Vp2m) and; vanilla using an inert tutor without cocoa interaction (Vp); each with five repetitions. For cocoa trees, Diameter at Breast Height (DBD<sub>1.5),</sub> total tree height, stem height, diameter and crown area were recorded. The same parameters were used to characterize associated trees. Subsequently, before and after establishing the vanilla crop in the agroforestry system, the amount of cocoa fruits was registered. Also, length and thickness of cuttings, number and length of sprouts (LB) and number of knots and leaves´ length were measured for this plant. Measurements were performed four times with an interval of 60 days starting at the time of establishing the vanilla. Non-significant statistical differences in cocoa production were observed. In contrast, the vegetative development variables of vanilla showed better adaptation regarding the vanilla treatment on cocoa (Vp/Tc), showing significant statistical differences (P≤0,05) when comparing LE, NN, NH and LB treatments. It is concluded that cocoa production is not affected when tutoring vanilla. In addition, the cocoa tutor provided good conditions for optimal vegetative development of vanilla.</p>

The effect of low-pressure plasma treatment on sunflower seed germination and sprouts growth rate

This study presents the influence of low-pressure plasma treatment on germination behaviour of sunflower seeds (Helianthus annuus). Both peeled and unpeeled seeds were treated with Ar plasma. The plasma was characterized using OES for understanding of physical and chemical species in the plasma. The physical properties and chemical properties of the samples were studied using SEM and water contact angle. Growth parameters including water uptake, seed germination, sprouts length and dry weight were also investigated. It was found that the surface roughness increased with the plasma-generating power. The water absorption decreased when the power increased and super hydrophilicity was achieved at a 100 W. The water uptake rate showed highest at 150 W plasma power. The highest rate of seed germination was found when the sunflower seeds were treated with plasma power of 150 W at 5-day sowing. Sprout length and dry weight increased with the plasma power as well. In summary, sunflower seeds treated by Ar plasma could improve seed germination rate, leading to significant change in wettability and growth parameter.

The effects of dielectric barrier discharge atmospheric air plasma treatment to germination and enhancement growth of sunflower seeds

This research was to study the effectiveness of dielectric barrier atmospheric plasma (DBD) on sunflower seed (Helianthus annuus). The DBD plasma was driven with a power of 90 watts. The samples consisted of five groups; control group, samples 1, 2, 3 and 4 being treated by DBD plasma with time limits of 30 s, 60 s, 90 s and 120 s, respectively. The physical properties and chemical properties were observed using SEM and water contact angle. Growth parameters were studied by the water uptake, seed germination, sprouts length and dry weight. Compared with the control sample the results showed the contact angle decreased and the wettability increased. The surface roughness in all conditions increased with increased power. With bigger power the water absorption decreased and super hydrophilic was achieved with 120 s treatment. The water uptake of plasma produced the best result using the DBD plasma treatment of 120s. The distribution of sprouts length and the dry weight both increased with increased power. The DBD plasma treatment on sunflower seeds improves seed germination rate and lead to a significant change in wettability and growth parameter.

The possibility of using the waste of the lignocellulosic hydrolysates biodetoxification as a seed treater of wheat varieties zoned in the West Siberian region of the of the Russian Federation

In the present study we investigated the possibility of using the waste of the lignocellulosic hydrolysates biodetoxification as a seed treater for wheat varieties zoned in the West Siberian region including the Tomsk region. The wastes are microbiocenoses of activated sludge, depleted during the detoxification process and capable of growing on toxic substrates. The study was conducted on three experimental objects and on wheat varieties Iren, Novosibirskaya 3, Novosibirskaya 51. For comparison, reference objects of biological preparations for plant protection Alirin-B, Fitosporin-M were used. The study showed that the waste of lignocellulosic hydrolysates biodetoxification had antifungal activity. The effectiveness of reducing the total contamination of seed infection in different experiments with experimental samples ranged from 52 to 82%. In some cases, the reference variants proved to be ineffective; the maximum effect on reducing the total seed contamination was achieved by Alirin-B and amounted about 30%. Seed treatment of wheat by the waste biodetoxification of different origins reduced the prevalence pathogens of seed wheat. At the same time the treatment did not significantly affect the increase in the parameters of plant growth and development (germination, length and weight of sprouts).

The Effect of Grain Germination to Improve Rice Quality

The stages of post-harvest processing have a very large influence on the yield and quality of rice produced. At the time of grinding grain, the high nutritional content in husks and bran are often wasted and used as animal feed. Through the process of germination of grain, the nutrient content in husks and bran can be used to increase the nutritional content of rice. The purpose of this study was to determine the effect of grain immersion and germination on the quality of rice produced. The method of this research was germinating grains by variation of soaking and incubation time. The duration of grain soaking, 12, 18, and 24 hours and incubation time period, 18, 24, and 30 hours. The parameters of this research, including ash content, protein, thiamin, and mineral content such as Fe and Mg. The data of this research was processed and tested by complete randomized design with one factorial. The result showed, ash and protein content of rice was a significantly different at 5% level on each treatment. For other parameters, thiamin was significantly different at 1% level on each treatment. The best treatment during the germinating grains process towards the quality of rice was soaking at 12 hours and incubation time 30 hours with length of grain sprout was 1 cm.

Influence of electronic irradiation on the affection of barley by root rot

The paper displays the results of the study of the effect of pre-sowing irradiation of spring barley of the Vladimir variety with a low-energy electron beam in a wide dose range from 1 to 8 kGy and a dose rate of 500 Gy/imp on seed infestation by root rot and sowing qualities. It has been shown that the development of the disease depends on both the radiation dose and the post-radiation period (PP). The most effective was the effect of irradiation on Helminthosporium sativum (synonymous with Drechslera teres) at PP of 4 days: there was observed a reduction in the damage degree by 33–48% and the disease incidence – by 29–46% at doses of 4–8 kGy. At the PP of 7 and 11 days, the irradiation of seeds with doses of 3–5 kGy reduces the degree of damage to the seedlings by Helminthosporium sativum by 30–37% and 28–41%, respectively. With an increase in PP up to 14 days, a decrease in the damage degree of seedlings by root rot by 33% was observed only at an irradiation dose of 4 kGy, which indicates a reduction in the efficiency of irradiation with increasing PP. The prevalence of the disease decreased at PP of 4 days by 28–46% (doses of 4-8 kGy), at PP of 7 days – by 30–37% (doses of 2–6 kGy), at PP of 11 days – by 26–48% (doses 3, 4 and 7 kGy) and PP 14 days – by 33% at a dose of 4 kGy. It has been established that electron irradiation stimulates laboratory germination of seeds at PP of 7 days by 22–25% at doses of 1, 2, 4 and 5 kGy and, on the contrary, reduces it by 19–24% at PP of 4 days and doses of 3, 5 and 8 kGy. At doses higher than 4 kGy, irradiation inhibits sprout length by 12–39%, roots – by 9–15%, and also at doses of 2–8 kGy, it reduces the wet weight of seedlings by 10–50%. Thus, it was shown that pre-sowing electron irradiation of spring barley of the Vladimir variety reduces the resistance of Helminthosporium sativum and can be used to combat the pathogenic microflora of the grain.