Risk Of Spread Research Articles

Reemergence of Oropouche Virus in the Americas and Risk for Spread in the United States and Its Territories, 2024.

Oropouche virus has recently caused outbreaks in South America and the Caribbean, expanding into areas to which the virus was previously not endemic. This geographic range expansion, in conjunction with the identification of vertical transmission and reports of deaths, has raised concerns about the broader threat this virus represents to the Americas. We review information on Oropouche virus, factors influencing its spread, transmission risk in the United States, and current status of public health response tools. On the basis of available data, the risk for sustained local transmission in the continental United States is considered low because of differences in vector ecology and in human-vector interactions when compared with Oropouche virus-endemic areas. However, more information is needed about the drivers for the current outbreak to clarify the risk for further expansion of this virus. Timely detection and control of this emerging pathogen should be prioritized to mitigate disease burden and stop its spread.

Societal Adaptation to Pandemic Challenges: Analysing the Role of Local Governance and Healthcare Systems in Madhya Pradesh

The COVID-19 pandemic posed unprecedented challenges, compelling communities globally to adapt rapidly to protect lives and maintain societal functions. This study examines the role of local governance and healthcare systems in Madhya Pradesh in addressing these challenges and fostering societal resilience. Drawing on quantitative data from government health agencies and qualitative insights from community feedback, this research explores how local governance structures facilitated public health response, resource allocation, and pandemic preparedness. Findings indicate that decentralized decision-making and robust healthcare infrastructure were instrumental in managing health crises, providing essential services, and supporting economic resilience at the grassroots level. Key results show that regions with stronger local governance exhibited a 30% lower infection rate compared to less governed areas, while areas with well-resourced healthcare systems saw a 20% higher recovery rate. Resource distribution, however, varied significantly, with urban areas receiving 60% more medical supplies than rural regions, creating disparities in healthcare access. Data analysis revealed that healthcare facilities that integrated digital solutions, such as telemedicine, were able to serve 45% more patients remotely, mitigating the risk of virus spread and reducing hospital overload. The study identifies the need for capacity-building initiatives, infrastructure strengthening, and data-driven decision-making to enhance future pandemic preparedness. Future prospects include continuous training programs for healthcare workers, increasing community awareness of health protocols, and leveraging technology for real-time data monitoring. These insights contribute to a comprehensive framework for building resilient healthcare systems in Madhya Pradesh, better prepared for future public health crises.

Spread of infectious diseases by microorganisms present in fomites

Fomites, inanimate objects that can carry infectious agents, play a critical role in the spread of infectious diseases. Despite advances in sanitation and hygiene practices, fomites remain a significant vector for the transmission of various pathogens, particularly in healthcare settings and densely populated environments. This paper reviews the mechanisms by which microorganisms are transmitted through fomites, the types of diseases commonly spread in this way and the factors that influence fomite-based transmission. Strategies for mitigating the risk of fomite-based disease spread are also discussed, highlighting the importance of targeted hygiene interventions, material design and environmental decontamination.

Autochthonous dengue outbreak in Lombardy, Italy: enhanced surveillance and vector control measures

Abstract Description of the problem On 18 August 2023, the first autochthonous case of dengue was registered in Lombardy, in a person living in a village in Lodi province. The case, a man with no history of travels prior to symptoms onset, developed flu-like symptoms on 3 August. After initial suspicion of WNV infection, he was confirmed to have a DENV-1 infection. Following the notification, measures to control the spread of the disease were activated, including alert of healthcare professionals, vector control activities, entomological monitoring and active case finding among household contacts and people living close to the case. Moreover, a serological screening was proposed to population of the entire municipality, along with a survey collecting data about travels abroad and symptoms. Results Forty-one confirmed and six probable autochthonous cases were identified. The last case developed symptoms on 20 October. Among confirmed cases, 24 were identified after admission to Emergency Department, 16 through community screening and one at the time of blood donation. 38/41 confirmed cases were symptomatic, with fever, rash and retro-orbital pain being the most common symptoms. Mean age was 55,5 years (3-88), while male: female ratio was 2:1. Hospitalisation was required for 7/41 cases. Community screening was offered from 25 August to 8 October and 416 individuals participated. Among them 10 tested positive for DENV IgG and 27 for DENV IgM (16 were subsequently confirmed as DENV cases, 6 remained probable and 5 were ruled out as non-cases). The primary case of the outbreak was not identified. Vector control activities and entomological monitoring were conducted on 21 August within 200-m radius area around the residence and places visited by index case. Subsequent interventions were conducted in all public areas and sensitive sites of the municipality. Lessons Enhancement of surveillance through community screening allowed identification of several additional autochthonous cases. Key messages • Strengthening of surveillance, vector control activities and entomological monitoring is crucial to prevent and control future outbreaks in non-endemic areas with stable presence of Aedes mosquitoes. • To minimize the risk of introduction and spread of dengue virus in non-endemic areas, it is essential to raise awareness among travellers and general population about preventive and control measures.

Strategy to develop and validate digital droplet PCR methods for global antimicrobial resistance wastewater surveillance.

According to World Health Organization (WHO), antimicrobial resistance (AMR) is currently one of the world's top 10 health threats, causing infections to become difficult or impossible to treat, increasing the risk of disease spread, severe illness, disability, and death. Accurate surveillance is a key component in the fight against AMR. Wastewater is progressively becoming a new player in AMR surveillance, with the promise of a cost-effective real-time tracking of global AMR profiles in specific regions. One of the most useful analytical methods for wastewater surveillance is currently based on real-time PCR (qPCR) and digital droplet PCR (ddPCR) technologies. As stated in the EU Wastewater Treatment Directive proposal, methodological standardization, including a workflow for method development and validation, will play a crucial role in global monitoring of AMR in wastewater. However, according to our knowledge, there are currently no qPCR and ddPCR methods for AMR surveillance available that have been validated according to international standard performance criteria. Therefore, this study proposes a workflow for the development and validation of PCR-based methods for a harmonized and global AMR surveillance, including the construction of specific sequence databases and microbial collections for an efficient method development and method specificity evaluation. Following this strategy, we have developed and validated four duplex ddPCR methods responding to international standard performance criteria, focusing on seven AMR genes (ARG's), including extended spectrum beta-lactam (blaCTX-M), carbapenem (blaKPC-2/3), tetracycline (tet(M)), erythromycin (erm(B)), vancomycin (vanA), sulfonamide (sul2), and aminoglycoside (aac(3)-IV), as well as one indicator of antibiotic (multi-) resistance and horizontal gene transfer, named the class I integron (intl1). The performance of these ddPCR methods was successfully assessed for their specificity, as no false-positive and false-negative results were observed. These ddPCR methods were also considered to be highly sensitive as showing a limit of detection below 25 copies of the targets. In addition, their applicability was confirmed using 14 wastewater samples collected from two Belgian water resource recovery facilities. The proposed study represents therefore a step forward to reinforce method harmonization in the context of the global AMR surveillance in wastewater. PRACTITIONER POINTS: In the context of wastewater surveillance, no PCR-based methods for global AMR monitoring are currently validated according to international standards. Consequently, we propose a workflow to develop and validate PCR-based methods for a harmonized and global AMR surveillance. This workflow resulted here in four duplex ddPCR methods targeting seven ARGs and one general indicator for mobilizable resistance genes. The applicability of these validated ddPCR methods was confirmed on 14 wastewater samples from two Belgian water resource recovery facilities.

Social listening applied to tailor communication on immunization in the Republic of Moldova.

Although the Republic of Moldova had good vaccination rates, anti-vax messages have recently begun to spread, eroding the population's confidence. Vaccination coverage against measles, mumps, and rubella at the target age of 12 months decreased from 95% in 2008 to 83% in 2021. The recent measles outbreak in Romania and three confirmed cases in Moldova, combined with the refugee crisis, have highlighted the importance of vaccination and sparked online conversations on this subject. A mixed-methods study was conducted, involving social listening activities on immunization using the Talkwalker software and qualitative analysis of the identified narratives. Out of 450 identified comments, 30 were selected for further analysis. Over 5 months, a total of 865 results were obtained, with an engagement rate of 6300. The peak in results occurred between 11 and 18 December, driven by the recent measles outbreak in Romania, which saw several confirmed cases and one death, as well as the first cases of measles in Moldova after 3 years. However, no significant increase in the engagement rate was recorded. Comments on immunization tended to reflect distrust in authorities and doctors, political and ideological views, and a lack of knowledge about the risks of vaccine-preventable diseases. The analysed conversations are assessed as having a low risk of spreading, though some employ techniques to influence or manipulate behaviours. The topic of vaccination is used to promote political ideologies through messages issued by the mass media. The information vacuum must be filled with messages designed to produce attitude change regarding vaccination.

EVIT-YOLOv8: Construction and research on African Swine Fever facial expression recognition

The threats of infectious diseases such as African Swine Fever, Swine Erysipelas, and Blue Ear Disease to the pig farming industry have been increasing year by year. Producers often face difficulties in diagnosis, leading to the misuse of measures and the spread of epidemics. Addressing this, the proposed EVIT-YOLOv8 model integrates the EViT module to surpass ViT limitations and incorporates the CBAM module for enhanced image feature representation. Employing the GIOU loss function ensures better precision in capturing facial expression features, yielding an impressive Mean Average Precision (mAP) of 86.6% in differentiation tasks. Specifically, in African Swine Fever facial expression recognition, the model achieves a remarkable Precision of 85.2%, outperforming YOLOv5, YOLOv7, and YOLOv8 models by 6%, 23.5%, and 7.3%, respectively. This provides pig producers with a precise diagnostic tool, mitigating the risk of epidemic spread due to misdiagnosis and facilitating effective prevention and control of infectious diseases.

Convertible lease risk spread modeling with correlation

Convertible lease risk spread modeling with correlation

Hidden pathogen risk in mature compost: Low optimal growth temperature confers pathogen survival and activity during manure composting

Livestock manure is a major reservoir for pathogens, posing significant environmental risks if used untreated. The efficacy of composting in fully inactivating pathogens remains controversial, particularly regarding the influence of their optimal growth temperature (OGT). This study investigated the composition and dynamic changes of pathogen communities and virulence factors (VFs) during the composting of chicken, bovine, ovine, and swine manure. We identified 134 pathogens across 16 composting piles, with ten pathogens exhibited increased abundance and transcriptional activity in curing phase. They included high-risk VFs-carrying pathogens, such as Mycolicibacterium thermoresistibile and Mycolicibacterium phlei, indicating the hidden pathogen risk in mature compost. Community-scale analyses revealed a linkage of these pathogens’ survival with their low OGT and an increased number of heat shock proteins (HSPs), enabling them to tolerate high temperatures and regrow. Integrating our data with prior composting studies, we found that the surviving pathogens express 42 VFs and their persistence in mature compost was a widespread issue, highlighting a greater risk of pathogen spread than previously thought. Finally, we compiled the 134 pathogens and 1009 VFs into a comprehensive Environmental Risk of Compost Pathogens (ERCP) catalog, providing a valuable resource for routine pathogen surveillance.

EPIZOOTIC LYMPHANGITIS - THE FORGOTTEN DISEASE OF UNGULATES

Epizootic lymphangitis, as a forgotten disease of ungulates, again attracts the attention of veterinarians and zoologists. This infection, caused by pathogenic microorganisms, leads to inflammation of the lymphatic vessels, which can cause serious complications and even death of the animal. Despite the rarity of cases in recent decades, epizootic lymphangitis leaves a significant mark on the history of veterinary medicine, causing alertness among specialists. The purpose of the review. To summarize current scientific data on the etiology, pathogenesis, diagnosis and prevention of equine epizootic lymphangitis in order to assess the risk of introduction and spread of the disease to disease-free territories. Results. The review presents the epidemiological characteristics of the etiological agent of the disease, possible main and intermediate reservoirs of the pathogen in nature, mechanisms of transmission of infection, promising directions for the development of prevention and treatment tools. Conclusion. The causes of epidemics remain a mystery, but the factors contributing to the spread of the disease range from lack of vaccinations to unfavorable conditions of detention. The fight against this disease should be carried out through vaccination, isolation and quarantine of sick animals, disinfection of animal housing and equipment, as well as surveillance.

The Determination of the Rapid and Effective Activity of an Air Sanitizer against Aerosolized Bacteria Using a Room-Sized Aerobiology Chamber.

Air sanitization is an important non-pharmaceutical intervention for mitigating the risk of indoor pathogen spreading. A dipropylene glycol-containing air sanitizer was tested against aerosolized Staphylococcus aureus and Klebsiella pneumoniae. The bacteria, suspended in a soil load, were aerosolized using a six-jet Collison nebulizer with pressurized air. The 25-m3 (~900 ft3) aerobiology chamber was maintained at 22 ± 2 °C and 50 ± 5% relative humidity per the U.S. Environmental Protection Agency's 2012 Guidelines on air sanitizers. An initial 2-min air sample was collected from the chamber using a slit-to-agar sampler containing 150-mm Petri plates, with Trypticase soy agar (TSA) containing neutralizers to quench the microbicidal activity of the air sanitizer, to determine the initial bacterial challenge in the air. The air sanitizer was sprayed into the chamber from pressurized cans. Additional air samples were collected from the chamber over 10 min to detect surviving bacteria. The TSA plates were then incubated aerobically at 36 ± 1 °C for 90 ± 4 h and scored for bacterial colony-forming units. A 30-s spray of the air sanitizer reduced infectious S. aureus and K. pneumoniae titers by 3.0 log10 (99.9%) in 3.2 ± 0.3 min and 1.2 ± 0.0 min, respectively. Based on these findings, the EPA granted registration of the air sanitizer as the first product of its kind for indoor air sanitization.

Cross border population movements across three East African states: Implications for disease surveillance and response.

The frequent population movement across the five East African Countries poses risk of disease spread in the region. A clear understanding of population movement patterns is critical for informing cross-border disease control interventions. We assessed population mobility patterns across the borders of the East African states of Kenya, Uganda, and Rwanda. In November 2022, we conducted Focus Group Discussions (FGDs), Key Informant Interviews (KIIs), and participatory mapping. Participants were selected using purposive sampling and a topic guide used during interviews. Key informants included border districts (Uganda and Rwanda) and county health officials (Kenya). FGD participants were identified from border communities and travellers and these included truck drivers, commercial motorcyclists, and businesspersons. During KIIs and FGDs, we conducted participatory mapping using Population Connectivity Across Borders toolkits. Data were analysed using thematic analysis approach using Atlas ti 7 software. Different age groups travelled across borders for various reasons. Younger age groups travelled across the border for education, trade, social reasons, employment opportunities, agriculture and mining. While older age groups mainly travelled for healthcare and social reasons. Other common reasons for crossing the borders included religious and cultural matters. Respondents reported seasonal variations in the volume of travellers. Respondents reported using both official (4 Kenya-Uganda, 5 Rwanda-Uganda borders) and unofficial Points of Entry (PoEs) (14 Kenya-Uganda, 20 Uganda-Rwanda) for exit and entry movements on borders. Unofficial PoEs were preferred because they had fewer restrictions like the absence of health screening, and immigration and customs checks. Key destination points (points of interest) included: markets, health facilities, places of worship, education institutions, recreational facilities and business towns. Twenty-eight health facilities (10- Lwakhakha, Uganda, 10- Lwakhakha, Kenya, and 8- Cyanika, Uganda) along the borders were the most commonly visited by the travellers and border communities. Complex population movement and connectivity patterns were identified along the borders. These were used to guide cross-border disease surveillance and other border health strategies in the three countries. Findings were used to revise district response and preparedness plans by strengthening community-based surveillance in border communities.

How Do We Analyze the Accident Causation of Shield Construction of Water Conveyance Tunnels? A Method Based on the N-K Model and Complex Network

In the construction of water conveyance tunnels with the shield method, accidents have occurred from time to time, such as collapses and explosions, and it is of practical significance to explore the cause mechanism of the accident. However, previous research has not considered the effects of dependence between risks on the risk spread. In response, we propose a method based on the Natural Killing Model (the N-K Model) and complex network theory to analyze the cause of shield construction accidents in water conveyance tunnels. By deeply exploring the transmission mechanism and action intensity between system risks, this method can scientifically clarify the accident cause mechanism and provide support for engineering construction safety management. The method constructs a risk index system. Secondly, we introduce the N-K model to reveal the risk coupling mechanism. Then, based on complex network theory, we construct the incident causation model and revise the node’s centrality with the coupling value. Finally, the network topology parameters are calculated to quantitatively describe the causal characteristics of accidents, revealing the risk evolution process and critical causes. The research results indicate that the key causes of accidents are failure to construct according to regulations, inadequate emergency measures, poor ability of judgment and decision-making, and insufficient understanding of abnormal situations. The front end of critical links is subject to human or management risks and should be carefully controlled during construction.

Determinants of malaria spread among under-five children in Nigeria: results from a 2021 Nigerian malaria indicator cross-sectional survey

BackgroundMalaria is a global disease burden, especially in Africa, with Nigeria having the highest prevalence of malaria, with approximately 50% affecting children. Children under five years of age are vulnerable to the risk of malaria spread. This research aimed to identify the determinants of malaria spread among children under the age of 5 in Nigeria.MethodsThis study used national malaria indicator survey data from 2021 (2021NMIS). The NMIS was implemented by the National Malaria Elimination Programme (NMEP), and data were collected between 12 October and 4 December 2021. This study included 3678 children, and data cleaning and analysis were performed via STATA version 17 software.ResultsThere was a positive association between Child’s age in months of 13–23 (AOR = 2.97; 95% CI = 1.62–5.45), 24–35 months (AOR = 2.64; 95% CI = 1.43–4.88),36–47 months (AOR = 2.18; 95% CI = 1.17–4.08) and months of 48–59(AOR = 2.82; 95% CI = 1.53–5.23), households headed by females (AOR = 0.71; 95% CI = 0.54–0.95),households with all children slept in mosquito nets last night (AOR = 2.43; 95% CI = 1.39–4.21), some children slept in the mosquito bed net (AOR = 2.83; 95% CI = 1.50–5.35) and households with no mosquito bed nets (AOR = 2.18; 95% CI = 1.22–3.88),mothers who agreed to have heard or seen malaria messages in the last 6 months (AOR = 1.32; 95% CI = 1.62–1.74),respondents with medium level of awareness of malaria prevention messages had (AOR = 2.35; 95% CI = 1.62–3.4), Children from North East (AOR = 0.7; 95% CI = 0.54–0.9), South-South (AOR = 0.65; 95% CI = 0.5–0.85) and South West (AOR = 0.52; 95% CI = 0.37–0.73) and malaria status of children under five years of age in Nigeria.ConclusionThe government and other concerned malaria prevention organizations should emphasize maternal education programs that are vital for malaria prevention, early symptom recognition, and timely treatment, empowering families to take proactive measures. Collaboration among health, education, and community organizations is also crucial for integrated malaria control and prevention.

Household expenditure on control of urban mosquitoes Aedes albopictus and Culex pipiens in Emilia-Romagna, Northern Italy.

In 2007, the first outbreak of Chikungunya in Italy generated great alarm, highlighting the health risks caused by exotic species recently introduced in Europe and the need to strengthen control actions against the vectors. Besides health risks, mosquitoes cause nuisance, and citizens are required to adopt control measures. While the economic aspects of mosquito control by public agencies have been investigated, the scientific literature on the costs of mosquito protection incurred by families is scarce. This study assessed the households' expenditure on protective measures against mosquitoes in Emilia-Romagna, a region in Northern Italy. A phone questionnaire survey was conducted to collect data on the annual expenditure incurred by households for self-protection against mosquitos in relation to the perceived level of nuisance and the household and dwelling characteristics. Univariate and multivariate analyses were conducted to identify the main determinants influencing such expenditure, which resulted affected by dwelling characteristics, presence of children under 6 years of age, and health concerns of family members. The average annual household expenditure was estimated at 84.63 euros, about 30 times higher than the expenditure per household supported by regional and local administrations for interventions against mosquitoes in public areas, as calculated in a previous study. Household expenditure is mainly aimed at providing a direct defense against mosquito bites (mosquito nets, adulticides, skin-on repellents, etc.) while spending for more effective measures addressed to reduce mosquito density results marginal: e.g., only 3.5% of the total expenditure was dedicated to larval control. Control activities that lower the mosquito density in both private and public areas could reduce the use of household insecticides in urban environments and the related costs, and the risk of spread of imported arboviruses as well.

Estimating the risk and spatial spread of measles in populations with high MMR uptake: Using school-household networks to understand the 2013 to 2014 outbreak in the Netherlands.

Measles outbreaks are still routine, even in countries where vaccination coverage exceeds the guideline of 95%. Therefore, achieving ambitions for measles eradication will require understanding of how unvaccinated children interact with others who are unvaccinated. It is well established that schools and homes are key settings for both clustering of unvaccinated children and for transmission of infection. In this study, we evaluate the potential for contacts between unvaccinated children in these contexts to facilitate measles outbreaks with a focus on the Netherlands, where large outbreaks have been observed periodically since the introduction of mumps, measles and rubella (MMR). We created a network of all primary and secondary schools in the Netherlands based on the total number of household pairs between each school. A household pair are siblings from the same household who attend a different school. We parameterised the network with individual level administrative school and household data provided by the Dutch Ministry for Education and estimates of school level uptake of the MMR vaccine. We analysed the network to establish the relative strength of contact between schools and found that schools associated with low vaccine uptake are highly connected, aided by a differentiated school system in the Netherlands (Coleman homophily index (CHI) = 0.63). We simulated measles outbreaks on the network and evaluated the model against empirical measles data per postcode area from a large outbreak in 2013 (2,766 cases). We found that the network-based model could reproduce the observed size and spatial distribution of the historic outbreak much more clearly than the alternative models, with a case weighted receiver operating characteristic (ROC) sensitivity of 0.94, compared to 0.17 and 0.26 for models that do not account for specific network structure or school-level vaccine uptake, respectively. The key limitation of our framework is that it neglects transmission routes outside of school and household contexts. Our framework indicates that clustering of unvaccinated children in primary schools connected by unvaccinated children in related secondary schools lead to large, connected clusters of unvaccinated children. Using our approach, we could explain historical outbreaks on a spatial level. Our framework could be further developed to aid future outbreak response.

Dirty lives, wild birds, clean places, and exceptional health: a critical discourse analysis of Danish and Norwegian news coverage of avian influenza risk

Avian influenza outbreaks are recurring worldwide. In Norwegian and Danish media, fluctuating attention has been paid to these outbreaks and the coverage that exists largely portrays the situation as a distant problem, limited to so-called non-Western countries. In the present article, we have analysed news articles from two Norwegian and two Danish newspapers published in 2006/2008, 2017 and 2021 using Critical Discourse Analysis (CDA). By analysing linguistic articulations of risk and blame for avian influenza in Norwegian and Danish media, we explore how this portrayal creates discursive distinctions between risk and blame ‘here’ in Europe and ‘there’ in Asian and African countries. We discuss these discursive contrasts in light of Bergamo’s theory of ideology of health progress and Douglas’ understanding of hygiene as cultural ideas. Our analysis reveals that dominant portrayals of avian influenza in the news media construct particular discourses of blame, risk, and responsibility for disease emergence and spread that are ideologically underpinned by an understanding of disease management, meat production, and animal health in Norway and Denmark as exceptional. We argue that this may have implications for perceptions of places at risk of emergence and spread of viruses and the nature of responses for pandemic preparedness and disease eradication.

Geographical distribution and molecular identification of cyst nematodes in Bajaur, Pakistan

Summary A survey conducted along the Panjkora river in Bajaur, Khyber Pakhtunkhwa (KPK), Pakistan, identified seven isolates of nematode populations belonging to the Heteroderidae family among the 25 soil and root samples examined. Within these isolates, three were classified as Heterodera avenae, H. filipjevi and H. zeae, while the remaining four were identified as Globodera pallida. The identification of these cyst nematodes involved a combination of morphological and morphometric analyses, complemented by molecular techniques employing newly designed primers to amplify diagnostic regions from any cyst nematode. This study marks the first molecular characterisation of H. avenae, H. zeae and G. pallida in Pakistan and the initial report of H. filipjevi in the country. The mapping of crop-growing regions with sampling points highlights areas at high risk for further pathogen spread.

Evil and allies: Opportunistic gulls as both spreaders and sentinels of antibiotic‐resistant bacteria in human‐transformed landscapes

Abstract Human‐transformed residuals, especially those derived from human waste (dumps), farmland, and livestock are involved in the emergence of antibiotic‐resistant bacteria (ARB) in the environment. Wildlife can act as vectors of ARB dispersal through different environments, but also as sentinels to detect the early spread and determine ARB sources. The development of integrated monitoring programmes focused on wildlife would help to anticipate the risks of ARB to humans and livestock. We used the yellow‐legged gull (Larus michahellis) as a model species to investigate and monitor the spatial patterns of ARB dispersal across an extensive farmland region located in northeastern Spain (Lleida). By integrating GPS tracking data and ARB clinical testing for 26 individuals within a network analysis framework, we modelled the risk of spatial pathogen spread through faeces during the bacteria‐transmission latency period (16 days after sample collection). Additionally, we created a connectivity network to determine the main sources of ARB in the area, focusing on three main habitats of special risk for infection: dumps, livestock facilities, and irrigation ponds. Seven individuals were infected by Escherichia coli, with one also co‐infected with Listeria monocytogenes and Salmonella spp. Potential pathogen dispersal distances ranged from 1.13 km to 23.13 km from the breeding colony. Our network analyses revealed 54 main nodes (i.e. high‐risk habitats recurrently visited by tracked gulls) and 1182 links among them. Our findings revealed a high degree of connectivity between the breeding area, located in a shallow lake, and nearby dumps, highlighting them as significant contributors to ARB dispersal. Synthesis and applications: The integration of GPS data, pathogen testing and network analyses can shed further light on pathogen dynamics by creating spatial risk maps and identifying ARB sources. In combination with complementary molecular epidemiology techniques within a One Health framework, our approach can emerge as an important tool for monitoring ARB dynamics within highly human‐transformed ecosystems. This may empower managers for the development of targeted ARB monitoring programmes and effective mitigation strategies, ultimately improving both animal and public health.

Evaluation of per- and polyfluoroalkyl substances (PFAS) toxic effects on the acute inflammatory response in the medicinal leech Hirudoverbana

Per- and polyfluoroalkyl (PFAS) substances are a large group of chemicals with elevated water and oil-resistance properties, widely implicated in various applicative fields. Due to the extensive use and high resistance to degradative factors, these compounds pose a significant risk of environmental spreading, bioaccumulating also in living organisms. In this context, despite many researches have been performed to demonstrate “legacy” PFAS harmfulness, only few data are still available about all the emerging fluorinated molecules, industrially introduced to replace the previous ones. For this reason, we proposed the medicinal leech Hirudo verbana as consolidated invertebrate model to assess the effects of four different PFAS (HFPO-DA, PFMoBa, PFOA and PFMOPrA) following freshwater dispersion. Morphological, immunohistochemical and molecular analyses demonstrate that, despite all the compounds basically induce an acute inflammatory and oxidative stress response, a different cellular and molecular response has been observed. Whereas for PFOA and PFMOPrA an increase in the tested concentration leads to a corresponding rise in the immune response, HFPO-DA and PFMoBa trigger an entirely opposite effect. Indeed, the significant recruitment of both granulocytes and macrophage like cells, typically involved in the removal of non-self, is inhibited with increasing concentrations of these compounds. The data collected revealed a different sensitivity of the leech immune system following PFAS exposure, requiring to deepen the current knowledge on the potential toxicity of these compounds.