Spodoptera Littoralis Boisd Research Articles

Effect of different temperature on the cotton leafworm Spodoptera littoralis Boisd (Lepidoptera: Noctuidae) toxicity by insecticides and the biochemical responses measurements

The influence of post-treatment temperature on the toxicities of insecticides against Spodoptera littoralis (Boisd.) larvae was evaluated in laboratory bioassays under different temperatures sets and enzymes contents like beta glucosidase, mixed function oxidase, lipase and the free amino acids. The thiamethoxam, imidacloprid, profenofos and malathion LC50 values in ppm decreased by increasing temperatures degrees but deltamethrin LC50 was slightly increased when temperature increased from 15 °C to 35 °C by 1.4 fold. Remarkably the toxicity increased with the temperature increase, except for deltamethrin. All the tested insecticides were significantly have positive correlation with temperature. Temperature coefficient were calculated for both one and total increments. Biochemical investigation of enzymes amount changes for thiamethoxam treatments at two sublethal concentrations and different temperature established were measured. MFO were generally increased at higher temperature and with increased concentrations. But the total amino acids decreased with concentration increase at both temperatures respectively and increased with temperature increase. The enzyme β-glucosidases and lipases recorded highest activity at higher temperature and lower activity at lower temperature of both thiamethoxam concentration respectively.

Performance of some new soybean genotypes against the infestation of the cotton leaf worm, Spodoptera littoralis Boisd

Cotton leaf worm, Spodoptera littoralis (Lepidoptra: Noctuidae) is considered one of the most destructive insect pests in Egypt. A field trail was conducted at the experimental farm of Nubaria agricultural research station, Behaira Governorate, Egypt, during 2021 and 2022 seasons to evaluate the performance of 14 new genotypes and two check varieties under the cotton leaf worn natural infestation conditions. The experimental design was a randomized complete block design (RCBD) with three replicates. The combined results revealed that there were substantial differences across genotypes for all evaluated parameters. The genotypes (H7L39/2015, H6L49, and H6L28) were considered as more resistant ones over the resistant check variety (Giza 111) which recorded the least infestation (25.86, 27.57 and 32.14 %, respectively), with feeding damage area of 18.52, 19.58 and 18.58 %, respectively. These genotypes gave the highest leaf content of P%, K%, total phenolic compounds, yield, and its components. The correlations between infestation% and leaflet area damage% or N all were positive on one another. All other correlations, with the exception of plant height and branches no plant-1, had negative effects on one another. The results proposed that the genotypes (H7L39/2015, H6L49 and H6L28) are promising and could be planted at Nubaria region and used in the breeding programs.

Utility of 5,5′‐Methylenebis(2‐hydroxybenzaldehyde) – cyanoacetohydrazide Hybrid as Scaffold for the Synthesis of Bis‐Heterocyclic Moieties and Study of Their Insecticidal Activity

AbstractThe cotton leafworm, Spodoptera littoralis Boisd. is a serious pest attacking a wide range of ornamentals and many field crops. It is well documented that some heterocyclic systems have efficient insecticidal activities. So novel hydrazide‐hydrazone namely, N′,N′′′‐((methylenebis(6‐hydroxy‐3,1‐phenylene))bis(methanylylidene))bis(2‐cyanoacetohydrazide) 3 was exploited as a target to synthesize bis‐heterocyclic moieties such as furan, pyridone, coumarin, thiazole and isoxazole, and also some bis‐heteroarylidenes including heterocycles as indole and pyrazole. The chemical structures of the synthesized compounds were established on the basis of elemental analyses and some spectroscopic tools. All the synthesized compounds were screened for the insecticidal efficacy against the 4th larval instar of Spodoptera littoralis (cotton leafworm). Manifestly, the data showed that compounds 9 and 14 possess remarkable potency but the rest of compounds showed no pronounced activity against cotton leafworm.

Effect of Exogenously Field Application of Jojoba Oil and Methomyl on Biochemical Constituents of Broad Bean (Vicia faba L.) Against Cotton Leafworm (Spodoptera littoralis Boisd.)

Effect of Exogenously Field Application of Jojoba Oil and Methomyl on Biochemical Constituents of Broad Bean (Vicia faba L.) Against Cotton Leafworm (Spodoptera littoralis Boisd.)

Plant Lectins as Insecticidal Agents Against Cotton Leafworm Spodoptera littoralisand Their Potential Applications in Crop Protection

Background: The production of insecticidal proteins is one of the greatest vital direct defense responses in plants against the attack by phytophagous insects. One particular class of the entomotoxic proteins present in many plant species is lectins that is used against a wide range of important insects. Aim: The insecticidal activity of two lectins from seeds of white kidney bean (Phaseolus vulgaris) and soybean (Glycine max) on the growth, development and survival of the cotton leafworm, Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) was studied. Methods: Lectin products were isolated and purified using ammonium sulfate precipitation. Insecticidal activity, growth-inhibitory and physiological effects on the differential haemocyte count of lectin products were evaluated against S. littoralis. In vivo effects of lectin products on some S. littoralis enzymes were also determined. Results: Lectin contents in 250 g seeds were 700 and 500 mg in soybean and white kidney bean, respectively. The total protein concentrations were 160-163 and 118-120 mg/mL in soybean and white kidney bean, respectively. The insecticidal activity of lectin products exerted a strong effect on the larval weight gain and the reduction of the weight reached to 75.25%. Furthermore, the other developmental stages of pupation and adult formation were also affected. An elongated larval duration was noticed since larval duration was 24.8 days compared with control (12.0 days). Complete pupation (100%) was recorded in the control; however, a significant decrease in pupation was found to be 54.0% in treated larvae. Lectin products increased the average number of 123.9 haemocytes (123.9) compared to the control (94.4 haemocytes/field). In addition, lectin products inhibited the activity of total proteases, α-amylase, GST and total esterases. Conclusion: It can be concluded that the lectins have high potential in pest control strategies based on their activity towards the cotton leafworm, S. littoralis. These findings suggest that lectin products are interesting tools for integrated pest management in important agriculture crops.

Effect of Plant Extracts of Withania somnifera (L.) Dunal. on Some Biological Performance of Cotton Leaf Worm (Spodoptera littoralis Boisd.)

Al-Jorany, R.S. and H.I. Al-Khazraji. 2021. Effect of Plant Extracts of Withania somnifera (L.) Dunal. on Some Biological Performance of Cotton Leaf Worm (Spodoptera littoralis Boisd.). Arab Journal of Plant Protection, 39(1): 22-28. This study was conducted to determine the effect of ethanol and hexane extracts of withania somnifera on the larvae of the cotton leaf worm Spodoptera littoralis when the 2nd and 4th larvae instars were fed on food treated with plant extract concentrations of 1.25, 2.5 and 5%. The results showed that the 2 nd instar larvae were the most susceptible than the late ones. The highest mortality rate reached 58.52% for the 2 nd instar larvae and 20.74% for the 4 th ones when 5% ethanol extract concentration was used. The results pointed to prolonged durations of the larval and pupal stages depending on the extract concentration used. The longest period of growth stage was found for the treatment with 5% hexane extract concentration. The fertility rate of adults emerged from treated larvae was decreased. The numbers of eggs laid was 670 eggs/female and the egg hatching rate reached 71% when 5% extract concentration was used to treat 2 nd larvae instars. Keywords: Extracts, Withania somnifera, cotton leaf worm, mortality rate, biological effects.

FIELD EVALUATION OF SELECTED OXADIAZINE INSECTICIDE AND BACTERIAL BIO-INSECTICIDES AGAINST COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISDUVAL) (LEPIDOPTERA: NOCTUIDAE) INFESTING SUGAR BEET ( BETA VULGARIS L)

In the two successive growing seasons, 2017 and 2018, the efficacy of newer chemistry insecticide, Avaunt® (Indoxacarb) and a commercial bio-insecticide, Protecto® ( B a cillu s t h u rin g ein sis kurstaki) was evaluated in the field for management of Cotton leafworm, Spodoptera littoralis Boisd. in Egypt. The tested compounds were applied at the recommended dose proposed by Agricultural Pesticide Committee (APC). Sugar beet was cultivated at Talaat-El Agamy Farm, Shenno country, Kafr El-Sheikh Governorate. The sugar beet variety (Karam) was cultivated by the end of September during the two seasons in an area of about 1100 fed. The field evaluation revealed that Avaunt® significantly reduced the larval population of S. littoralis compared to the untreated area. Protecto® caused a significant reduction in insect infestation up to five days post treatment. Both Avaunt® and Protecto® can be used effectively by farmers as a component of integrated management of cotton leafworm in Egypt.

Antioxidant Activity and Biological Studies of Two Medicinal Plant Extracts on Spodoptera littoralis (Boisd)

Control management of Spodoptera littoralis (Boisd) infestation has been achieved by using chemical insecticides. However, the environment-friendly methods without unwanted side effects of these chemicals are becoming very important in modern pest management strategies. Natural products including plant ethanolic extracts and essential oils are some of the alternative approaches in pest control. The essential oils were extracted and their chemical composition was identified using a GC/MS spectrometer. Therefore, the present study was designed to evaluate the antioxidant activity of various extracts of sage and thyme plants by 2, 2-diphenyl-1-picrylhydrazil (DPPH) method. The obtained results revealed that concentrations at 25, 50, 100 and 200µg/ml of essential oils and ethanolic extract from both plants were more efficient in scavenging free radicals after 30 min when compared with butyl hydroxyl toluene(BHT) as synthetic standard. The concentrations 100 and 200µg/mL from ethanolic and essential oils were having higher activity than other extracts and standard .Furthermore, Sage and thyme extracts were investigated for their toxicity against cotton leafworm, S. littoralis in the laboratory, the percentages of cumulative mortality of larvae as well as the latent effects of the 4th instar of S. littoralis four concentrations (25, 50, 100 and 200 µg/mL) were applied. It was found that ethanolic extract of two plants was more efficient than essential oils on different stages of S. littoralis. The results showed that there were highly significant differences between all treatments and control some biological aspects.

Evaluation of the toxic and physiological activities of Precocene I (Anti-juvenile hormone agent) against Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae)

Evaluation of the toxic and physiological activities of Precocene I (Anti-juvenile hormone agent) against Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae)

Effect of Solitary Versus Group Rearing on Biological Aspects of Theridion spinitarse Cambridge (Araneae: Theridiidae) on Spodoptera littoralis Boisd. Larvae

Biological aspects of solitary versus group rearing of Theridion spinitarse Cambridge (Araneae: Theridiidae) on Spodoptera littoralis Boisd. Larvae under 30°C and 60-70% R.H. were considered. Developmental durations did not differ between the two situations. Males and females reached maturity after the fifth spiderling. Life cycle was 60.89, 63.33, 55.1 and 62.1 days for males and females under solitary and group rearing, respectively. Adult longevity, lifespan, fecundity of female and food consumption were recorded. Results indicated that group rearing favored better biological aspects than individual rearing under same conditions as well as female's specific data. Pre-oviposion period was shorter in group rearing compared with solitary one (i.e. 12.2 versus 25.73 days, respectively). Oviposion period was longer for group rearing with higher fecundity per female (i.e. 62.3 versus 41.82 days). Fecundity was 158.30 versus 99.00 eggs/ female, respectively. It was concluded that better emulation of natural conditions for the organism results in more accurate biological aspects data.

Effect of Some Compounds Against Pupae of Spodoptera littoralis (boisd.) With Some Depths and Ages

The effect of some compounds Naoumil 90% SP, Diflorate 25%WP, Match 5% EC,Extreme 36% Sc and Pyrethrum 5%EC were tested against immerging newly-formed, 3 days and 6-day-old pupae of the cotton pest Spodoptera littoralis (Boisd) in sand-soil at a depth of 5, 10 and 15 cm in the laboratory at 25±1°C and 70±5% RH. Data revealed the compounds on pupae of Spodoptera littoralis (Boisd)after newly formed with depth 10 cm, results indicated that Pyrethrum 5% EC (Pyrethrins) 100%; Diflorate 25%WP (Diflubenzuron) & Naoumil 90% SP (Methomyl) gave 80%; Extreme 36% Sc (Methoxyfenzide + Spinetoram) 60% malformation and Match 5% (Lufenuron) Zero% respectively compared with control at depth of 10 cm. While at depth of 15 cm, obtained results indicated that : Pyrethrum 5% EC (Pyrethrins) 100% malformation of adults emergence. While Diflorate 25%WP (Diflubenzuron) exhibited 80% malformation ; Extreme 36% Sc (Methoxyfenzide + Spinetoram) 20% and Naoumil 90% SP (Methomyl ) & Match 5% (Lufenuron) zero% after one day treatments respectively compared with control. It is concluded that treated sandy-soil with some compounds such as (Methomyl, Diflubenzuron, Lufenuron and Pyrethrins), before cultivation an effective integrated pest management method to control S. littoralis in Egypt.

Comparative chemistry and biological properties of the solid residues from hydrodistillation of Spanish populations of <em>Rosmarinus officinalis</em> L.

Solid residues from the hydrodistillation of selected Spanish populations of rosemary (Rosmarinus officinalis L.) have been analyzed for their polyphenol composition, and antioxidant and bioplaguicide activities. The objective was to evaluate and select the most suitable plant materials as sources of natural antioxidants and crop protectants. Total polyphenol content and polyphenol composition of rosemary populations were very dependent on the growth location: populations from Aranjuez showed a higher content of total polyphenols and were richer in rosmarinic acid as compared with their equivalent populations from Cuenca, whereas these latter were characterized by an overall higher content in genkwanin and carnosol. Most of the antioxidant activities were highly correlated with the total content of polyphenols although some polyphenols like carnosic acid and carnosol seemed to favor such activities. The extracts from R. officinalis were strong antifeedants against Leptinotarsa decemlineata Say and moderate against Spodoptera littoralis Boisd and Myzus persicae Sulzer, according to their feeding ecologies. The biological effects of the active samples cannot be accounted by their chemical composition, suggesting additive or synergistic effects. Both the phytotoxic and stimulating effects on Lactuca sativa L., and Lolium perenne L. leaf and/or root growth were observed.

Direct Contact - Sorptive Tape Extraction coupled with Gas Chromatography - Mass Spectrometry to reveal volatile topographical dynamics of lima bean (Phaseolus lunatus L.) upon herbivory by Spodoptera littoralis Boisd.

BackgroundThe dynamics of plant volatile (PV) emission, and the relationship between damaged area and biosynthesis of bioactive molecules in plant-insect interactions, remain open questions. Direct Contact-Sorptive Tape Extraction (DC-STE) is a sorption sampling technique employing non adhesive polydimethylsiloxane tapes, which are placed in direct contact with a biologically-active surface. DC-STE coupled to Gas Chromatography – Mass Spectrometry (GC-MS) is a non-destructive, high concentration-capacity sampling technique able to detect and allow identification of PVs involved in plant responses to biotic and abiotic stresses. Here we investigated the leaf topographical dynamics of herbivory-induced PV (HIPV) produced by Phaseolus lunatus L. (lima bean) in response to herbivory by larvae of the Mediterranean climbing cutworm (Spodoptera littoralis Boisd.) and mechanical wounding by DC-STE-GC-MS.ResultsTime-course experiments on herbivory wounding caused by larvae (HW), mechanical damage by a pattern wheel (MD), and MD combined with the larvae oral secretions (OS) showed that green leaf volatiles (GLVs) [(E)-2-hexenal, (Z)-3-hexen-1-ol, 1-octen-3-ol, (Z)-3-hexenyl acetate, (Z)-3-hexenyl butyrate] were associated with both MD and HW, whereas monoterpenoids [(E)-β-ocimene], sesquiterpenoids [(E)-nerolidol] and homoterpenes (DMNT and TMTT) were specifically associated with HW. Up-regulation of genes coding for HIPV-related enzymes (Farnesyl Pyrophosphate Synthase, Lipoxygenase, Ocimene Synthase and Terpene Synthase 2) was consistent with HIPV results. GLVs and sesquiterpenoids were produced locally and found to influence their own gene expression in distant tissues, whereas (E)-β-ocimene, TMTT, and DMNT gene expression was limited to wounded areas.ConclusionsDC-STE-GC-MS was found to be a reliable method for the topographical evaluation of plant responses to biotic and abiotic stresses, by revealing the differential distribution of different classes of HIPVs. The main advantages of this technique include: a) in vivo sampling; b) reproducible sampling; c) ease of execution; d) simultaneous assays of different leaf portions, and e) preservation of plant material for further “omic” studies. DC-STE-GC-MS is also a low-impact innovative method for in situ PV detection that finds potential applications in sustainable crop management.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0487-4) contains supplementary material, which is available to authorized users.

Field evaluation and biochemical studies of novel insecticide on the cotton leafworm, Spodoptera littoralis (Boisd).

Field evaluation for the efficacy of indoxacarb, spinetoram and methoxyfenozide against larval instars of S. littoralis, infested clover plants Trifoliuma lexandrium was studied at two different Governorates (Al- Qalyubia and El- Fayoum) during cultivation season 2012 - 2013.All three insecticides, of indoxacarb, spinetoram and methoxyfenozide had no great difference among themselves in reducing the larval population of S. littoralis with difference in the time needed to induce this reduction according to the mode of action of these insecticides.The initial reductionwas recorded after 1 day from spraying for indoxacarb and spinetoram while after 3 days from spraying for methoxyfenozide. Indoxacarb was the most effective insecticide followed by methoxyfenozide and spinetoram.Under semi-field conditions, results showed that, at Al- Qalyubia Governorate, persistence of indoxacarb is higher than other tested insecticides while, the half life of all tested compounds is short at El-Fayoum Governorate.Tolerance to the toxicity of the three tested insecticides increased as the larvae grew older from 2 nd to 4 th instar.Biochemical clarification was carried out in an attempt to disclose the effect of sub-lethal concentration (LC50) of the three tested insecticides; on haemolymph contents of 6 th instar larvae of S. littoralis which was treated as 4 th larval instar with LC50 of the tested insecticides and their effects on main components of insect metabolites and changes in protein patterns.

Acute, synergistic and antagonistic effects of some aromatic compounds on the Spodoptera littoralis Boisd. (Lep., Noctuidae) larvae

The efficacy of 30 aromatic compounds and their mutual binary combinations was tested for acute toxicity against the larvae Spodoptera littoralis. Based on a comparison of the lethal doses, the thymol, carvacrol and trans-anethole were selected as the most effective, and LD50 was estimated for them at 9, 15 and 18μg/larva, respectively, and LD90 at 28, 35 and 41μg/larva, respectively. In total, 435 binary combinations were tested, of which 135 combinations showed a significant synergic effect, while 150 combinations showed a significant antagonistic effect on mortality. Only 6 substances were identified as being able to create a synergic effect with more than 20 substances: γ-terpinene, limonene, p-cymene, trans-anethole, borneol and camphor. l-carvone and gallic acid created an antagonistic effect with the highest frequency.The highest synergic effect was achieved for the combination of camphor and borneol, with the place of expected mortality estimated at 4.8% and mortality at 88.6%. In order to verify the synergic interaction of camphor and borneol, the effectiveness in terms of acute toxicity for S. littoralis larvae was determined for three fundamental mixing ratios of the substances. Significantly different LD50 values (88 and 134μg/larva, respectively) were found for camphor/borneol mixtures prepared in the ratios 2:1 and 1:1, respectively.The information learned can thus be used in the development of new botanical insecticides based on EOs, and particularly in the creation of formulations.

Polyphenol composition, antioxidant and bioplaguicide activities of the solid residue from hydrodistillation of Rosmarinus officinalis L.

Rosemary is one of the most important aromatic plants in terms of commercialization of essential oil. Nevertheless, a large amount of solid residue with a considerable content of bioactive compounds is generated during the hydrodistillation of the essential oil. In this work we have identified the major phenolic compounds present in such residue and evaluated its antioxidant and bioplaguicide activities. Antioxidant activity was determined by several in vitro methods, such as the β-carotene/linoleate model system, reducing power, DPPH radical scavenging, and iron and copper ion chelation. Bioplaguicide activity was investigated by means of the antifeedant effects against Leptinotarsa decemlineata Say, Spodoptera littoralis Boisd. and Myzus persicae Sulzer; and the phytotoxic activity against Lactuca sativa L., Lycopersicon esculentum L. and Lolium perenne L. Solid residue extract showed very high antioxidant activities, comparable to an extract from red grape pomace that was used as a natural antioxidant standard. It also showed strong antifeedant activity against L. decemlineata, S. littoralis and M. persicae with effective doses within the range of model antifeedants as well as limited phytotoxic effects on L. sativa, SL. esculentum and L. perenne. These results suggest a potential use of the solid residue from the hydrodistillation of rosemary as source of antioxidants and natural crop protectants.

The Effect of Water Hyacinth, Eichhornia Crassipes, and King-Bo Compound on Some Biological and Biochemical Aspects on Cotton Leaf Worm Spodoptera littoralis (Boisd).

The Effect of Water Hyacinth, Eichhornia Crassipes, and King-Bo Compound on Some Biological and Biochemical Aspects on Cotton Leaf Worm Spodoptera littoralis (Boisd).

Insecticidal effect of furanocoumarins from fruits of Angelica archangelica L. against larvae Spodoptera littoralis Boisd.

The essential oil (EO) and extracts (EXs) obtained from the seeds of Angelica archangelica were used to determine the efficacy in terms of chronic and acute toxicity, antifeedancy and growth inhibition of Spodoptera littoralis larvae. The EO and the EXs were analyzed by GC/MS and HPLC. The EO contained β-phellandrene as the major substance (60%). Furanocoumarins were identified in the EXs obtained using organic solvents, with the majority content of imperatorin (50–56%). Their varying efficacy was caused by the different composition of the EO and EXs obtained. Significant acute toxicity was caused only by the EO (LD50 96μg/larva). Significantly higher chronic toxicity was found for the EXs obtained using organic solvents (LD50 was estimated at 0.32, 0.82 and 0.52mgg−1 for benzene, acetone and methanol, respectively), compared to the EO (LD50 7.53mgg−1). All the tested EXs and the EO caused growth inhibition. The highest larval growth inhibition was caused by the benzene extract, with ED50 estimated at 2.4μgg−1. All EXs and the EO also showed antifeedant activity. However, the benzene extract showed the highest efficacy (ED50 0.31μgcm−2), while the least efficacy was shown by the water extract (ED50 1.92μgcm−2). In order to determine the dependence of biological efficacy of the EXs, LD50 and ED50 values were subjected to regression and correlation analysis. The analysis showed dependence between the total content of furanocoumarins in the extracts and chronic toxicity (R2=0.8451; P<0.01) or larval growth inhibition (R2=0.8941; P<0.01). This dependence was also confirmed for all individual furanocoumarins except bergapten, which showed no dependence.Based on a comparison of the determined efficacy and yield of the extracts, extracts from A. archangelica seeds obtained using methanol can be recommended as suitable for the development of botanical insecticides against S. littoralis larvae.

Toxical Effect of Peganum harmala L. Leaves on the Cotton Leaf Worm, Spodoptera littoralis Boisd and its Parasitoids Microplitis rufiventris Kok

The leaf extract and its fractions of Peganum harmala L. have shown pronounced mortal effect, decreased percent pupation and adult emergence of the cotton leaf worm, Spodoptera littoralis Boisd. The third instar larvae fed for two days on treated leaves were more susceptible to plant extract and its ethyl acetate and chloroform fractions. The active lowest concentration (5%) of the leaf fractions of P. harmala showed significant effect on the percentage of emerged adult parasitoids, Microplitis rufiventris Kok. GC/MS analysis showed the major constituent in ethyl acetate fraction was (23S) ethylcholest-5-en-3 beta-ol (28.04%) while those of chloroform fraction were hydroxyfuranocoumarin (Bergaptol) (15.68%), piperidinone (12.08%), thymol (11.82%), phosphoric acid, tributyl ester (9.80%) and trimethyl-nonenol (9.66%). The medicinal plant P. harmala could be carefully applied in integrated pest management due to its strong effect on cotton leaf worm pest.

Antifeedant activity of some Tanacetum species and bioassay guided isolation of the secondary metabolites of Tanacetum cadmeum ssp. cadmeum (Compositae)

Antifeedant activities of Tanacetum cadmeum ssp. cadmeum, Tanacetum parthenium, Tanacetum corymbosum ssp. cinereum, Tanacetum chiliophyllum var. chiliophyllum, Tanacetum kotschyi and Tanacetum cadmeum ssp. orientale were assessed on Spodoptera littoralis Boisd. (Lep: Noctuidae) third instar larvae. The highest antifeedant activities were found as 62 and 55.05% in the extracts of MeOH with T. cadmeum ssp. cadmeum aerial parts, MeOH with T. corymbosum ssp. cinereum flowers, respectively. Bioassay guided investigations on Tanacetum cadmeum ssp. cadmeum afforded three eudesmanolides; artesin (1), taurin (2), and artemin (3), three germacranolides; tavulin (4), tanachin (5), and tamirin (6), the coumarin; scopoletin (7), and two flavonol derivatives; tanetin (6-hydroxykaempferol 3,7,4′-trimethylether) (8) and 6-hydroxykaempferol 3,6-dimethylether (9). The structures of the compounds were elucidated by spectral methods, such as IR, UV, 1H NMR and 13C NMR (COSY, APT, DEPT, HMQC, HMBC, NOE, X-ray, EIMS, CIMS and HRMS). In this paper, the antifeedant activities of some Tanacetum species and the compounds isolated from T. cadmeum ssp. cadmeum are reported.