Turpentine Research Articles

Comparative Analysis of Essential Oil Composition in Pinus sylvestris, Leucadendron argenteum, Impatiens balsamina and Abies sibirica

Background: Essential oil components play an important role in food flavoring industries and chemical industries. Essential oil from Pinus sylvestris (pine oil, scotch pine oil, Turpentine Oil), Leucadendron argenteum (silver fir needle oil), Impatiens balsamina (balsam fir needle oil), and Abies sibirica (siberianfirn needle oil) contains numerous active ingredients that help to develop drug preparation. Objective: To compare the phytoconstituents present in the essential oil of Pinus sylvestris (wood, cone, needle), Leucadendron argenteum (needle), Impatiens balsamina (needle) and Abies sibirica (needle). Methods: This study concentrates on analyzing the natural flavor in six essential oils of Pinus sylvestris (wood, cone, needle), Leucadendron argenteum (needle), Impatiens balsamina (needle), and Abies sibirica (needle) subjected to gas chromatography analysis with the condition of 40ºC for 2 min, 40ºC to 180ºC @ rate 15 ºC; 180ºC to 230ºC at 10ºC/min and final hold at 230ºC for 2 min. AFID detector was used. Results: The results showed the presence of several organic flavoring compounds, especially terpenes and terpenoids. Alpha pinene are the major flavoring molecules present in all the selected oils with a higher percentage. Alpha pinene (60.25 % and 50.37 %) was found to be highest in turpentine and scotch pine oils respectively. Alpha terpineol (51.73 %), limonene (34.33%), betapinene (36.12 %), and bornyl acetate (35.94 %) were found higher levels in Pinus sylvestris, Leucadendron argenteum, Impatiens balsamina and Abies sibirica respectively. Alpha-pinene, betapinene, alpha terpineol, beta phellandrene, alphafenchene, borneol, limonene, camphene, bornyl acetate, myrcene, and delta 3 carene are the most common phytocompounds found in the essential oils. Conclusion: Alpha pinene, beta pinene, and limonene were present in all four plants Pinus sylvestris, Leucadendron argenteum, Impatiens balsamina and Abies sibirica and also utilized in aromatherapy and the perfume industry. conclusion: Alpha pinene, beta pinene and limonene were present in all four plants Pinus sylvestris, Leucadendron argenteum, Impatiens balsamina and Abies sibirica and also utilized in aromatherapy and in perfume industry.

Optimisation of performance and emission parameters of a CI engine fuelled with kapok oil methyl ester–turpentine oil blends and diethyl ether additive

The article aims to investigate the characteristics of compression ignition (CI) engines fuelled with blends of kapok oil methyl ester (KME) and turpentine oil (TO) with the inclusion of diethyl ether (DEE) as a fuel additive and the RSM has been used to analyse the engine characteristics. The design matrix is created for 50% to 100% loading and 30% to 70% blending in TO with kapok methyl ester. The experiment was conducted on a water-cooled 5.2 kW CI engine and 30%, 50%, and 70% of KME and TO blend. According to the test results, 50–50 KME and TO have a brake thermal efficiency of 29.13%. Another design matrix was designed for a load range of 50% to 100% and DEE of 0% to 20% in a 50 KT blend. The 50 KT blend with 20% DEE has the highest brake thermal efficiency (30.30%) and the lowest brake-specific energy consumption. The emissions of carbon monoxide, hydrocarbons, and smoke decrease to 8.25 g/kWh, 0.13 g/kWh, and 57%, respectively. The combustion process leads to the highest peak pressure and net heat release rate, which are 72 bar and 74.93 kJ/OCA, respectively. The outcome is 50–50% turpentine and kapok oil methyl ester blends, with 20% DEE have improved the performance and reduced the emissions.

Bio-derived graphitic carbon microspheres: A green approach for high-frequency microwave absorption

Bio-derived carbon materials have emerged as a sustainable alternative to non-renewable petroleum-based carbon sources, thanks to their reproducibility and environmental benefits. There is a strong demand for developing and efficiently utilizing carbon-based microwave absorbing materials (MAMs) that are lightweight, require low filler loading, and offer broadband absorption for electromagnetic wave (EM) applications. In this context, we successfully synthesized uniform carbon microspheres from the natural precursor turpentine oil using the chemical vapor deposition (CVD) technique at various temperatures. The sample prepared at 1000 °C (GC-1000) exhibited a uniform carbon microsphere morphology, as confirmed by XRD and Raman spectroscopy, and demonstrated extremely good microwave absorption properties. With a low filler loading of just 5 wt%, the GC-1000 sample achieved a minimum reflection loss (RL) of −39.77 dB at 10.21 GHz and an effective absorption bandwidth of 2.51 GHz at a matching thickness of only 2.8 mm. Additionally, this sample showed a total shielding effectiveness (SET) of −44.74 dB, surpassing the threshold required for commercial applications. The graphitic phase formation, confirmed by XRD and Raman analysis, acts as a conductive trap for electromagnetic radiation, and the high surface area of the uniform carbon microspheres facilitates multiple internal reflections, enhancing overall microwave absorption performance in the X-band region. Our lightweight, durable, bioderived carbon microspheres, synthesized through a cost-effective and scalable process, show significant potential for EM wave absorption in military and electromagnetic compatibility (EMC) devices.

THE EFFECT OF COPAL RESIN AND TURPENTINE OIL AS TACKIFIER ON THE ADHESIVES QUALITY BASED OF LIQUID RUBBER COMPOUND

This research aims to develop adhesives from liquid rubber compounds with a variety of tackifiers, a combination of copal resin, and turpentine oil. It also aims to characterize the adhesives to determine the quality of the adhesives produced. The research comprised several stages, including the preparation of rubber additive dispersions and solutions, formulation of liquid rubber compounds and adhesives, fabrication of test pieces, and subsequent characterization. The liquid rubber compound is made by mixing concentrated latex with rubber additives, which include KOH as a stabilizer, stearic acid and ZnO as an activator, BHT as an antioxidant, CaCO3 as a filler, MBTS and TMTD as an accelerator, and sulfur as a vulcanizer. The adhesives are made by mixing liquid rubber compound with tackifier material with various combinations of copal resin/turpentine oil, namely F1 (0 gram/4 gram); F2 (1 gram/3 grams); F3 (2 grams/2 grams), F4 (3 grams/1 gram), and F5 (4 grams/0 grams). Adhesives characterization was carried out by testing pH, viscosity, adhesives strength, FTIR, XRD, and thermal analysis. The research results showed that the best conditions were obtained on F4 adhesives with an adhesives strength value of 1.7452 N/mm, viscosity of 1100 cP, and pH 4.6. In this condition, the resulting adhesives meet the requirements for adhesives in accordance with SNI 06-6049-1999 and SNI 12-7195-2006.

Formulation and evaluation of anti-rheumatoid arthritis effects of Ag nanoparticles containing Allium sativum L. Leaf extract

Rheumatoid arthritis is a persistent inflammatory disorder that typically impacts individuals aged 20 to 40. It is characterized by inflammation of the synovial membrane, degradation of cartilage, and joint pain among its various symptoms. Researchers have recently developed, analyzed, and altered biomarkers and nanomaterials that are frequently employed to improve the diagnostic and therapeutic approaches for rheumatoid arthritis. Here, we have created and formulated a novel therapeutic agent that incorporates silver nanoparticles@Allium sativum L. leaves to treat arthritis. The characterization of silver nanoparticles (AgNPs) was performed utilizing various techniques. The FE-SEM and TEM images demonstrated that the majority of these nanoparticles exhibited a spherical shape (10 to 50 nm). Additionally, a notable peak at a wavelength of 403 nm in the UV–Vis spectrum confirmed the successful formulation of the Ag NPs. The antioxidant assay revealed that the IC50 values for the silver nanoparticles@Allium sativum L. and BHT in relation to DPPH were 21 and 44 µg/mL, respectively. In vivo assessments were performed utilizing models involving formaldehyde, CFA, and turpentine oil at various dosage levels. Silver nanoparticles@Allium sativum L. exhibited anti-arthritic properties with the most significant potential observed at 1 mg/kg. Data from the CFA model revealed a more substantial protective effect on arthritic lesions and alterations in body weight. The recent results indicates the recovery of rheumatoid arthritis criteria including synovial cavity swelling and edema inhibition percentage in turpentine test, paw diameter in CFA test and paw edema in formaldehyde test. In conclusion, these data support the administration of silver nanoparticles as potent therapeutic agents for rheumatoid arthritis treatment in humans.

Design and Optimizing of Precision Seeder of Pinus sylvestris var. mongolica L. Bud-Seeds for Containerized Nursery Production

Mongolian pine holds immense ecological and economic values as a vital biobased material. Its wood fiber, rosin and turpentine are important raw materials suitable for multiple industrial applications, it also exhibited soil and water conservation capabilities. To improve its seeding efficiency, this study designed a precision seeding machine for Mongolian pine (Pinus sylvestris var. mongolica L.) bud-seeds, and optimized the technical seeding parameters, which utilized a combined vibration-air blowing fluidization device to overcome seed flowability limitations and facilitate accurate seed selection. A particle model was established based on the seeds’ physical characteristics, and EDEM software was utilized to simulate the spatial distribution of seed particles under various seeding parameters through seeding experiments. The optimal air pressure for the combined seed supply was approximately 0.24 kPa, with a frequency of 20 Hz and an amplitude of 5 mm, to obtain an optimal spatial distribution of the seed particles. The most effective seeding area for seed adhesion was determined to be 5–15 mm from the bottom of the seeding plate. When the seed suction negative pressure is set to 6.7 kPa, with an upper hole diameter of 0.9 mm, a lower hole diameter of 3 mm, and a hole depth of 1.5 mm, the seedling emergence rate reached 89.06%, and the fastest emergence time was 2 days. One month after sowing, the seedlings exhibited a height growth rate of 16.1%, and a ground diameter growth rate of 20.9%. This study demonstrates the effectiveness of the precision seeding machine and optimized parameters in achieving efficient and accurate sowing of Mongolian pine bud-seeds. This work provides basic technical and data support for advancing the development and sustainable utilization of Mongolian pine as a vital biobased material resource.

Effect of hydrogen induction on CI engine characteristics fuelled with kapok oil methyl ester-turpentine blend with diethyl ether

This study explores the impact of hydrogen induction and diethyl ether (DEE) additives on the performance of a dual-fuel CI engine. The primary fuel blend comprises 50% kapok oil methyl ester and 50% turpentine oil (50KT) by volume. Hydrogen gas was inducted at a flow rate of 6–10 lpm, and while DEE was added with energy share ratios of 10% and 20%, accompanied by 10 lpm hydrogen induction. The engine was tested at loads ranging from 25 to 100%. Brake thermal efficiency increases significantly with 10 lpm of hydrogen induction while operating on the 50KT blend, increasing from 26.7% to 30.2%, 12% higher than the base 50KT. DEE with 10 lpm hydrogen and base fuel 50KT improves efficiency by 20.22%, compared to 32.2% for the base 50KT blend. The addition of 20% DEE reduced emissions of CO, hydrocarbon, and smoke by 41.12, 23.08, and 7.06%, respectively, while NOx increased by 19.96%. Hydrogen induction improves combustion by enhancing cylinder pressure and HRR by 7.1% and 7.3%, respectively, compared to diesel. The lowest entropy generation rate was 0.025 kW/K for maximum sustainability index and exergy efficiency of 2.26 and 56%, respectively. These outcomes indicate that hydrogen induction and DEE addition could improve performance while decreasing emissions.

Determinant analysis of oil agglomeration of coal fines using multiple regression and neural network models

ABSTRACT Oil agglomeration has gained importance owing to its simplicity and efficiency in beneficiating coal fines. In this study, high ash coal samples taken from six mines in India were put through an oil agglomeration process to investigate the usage of castor oil or blend of castor and turpentine for recovery of coal fines from coal-water slurry. The performance of the process has been evaluated based on ash rejection [AR (%)] and yield (%). Various Statistical analyses were carried out to investigate the role of various process parameters, such as PD, OD, AT, and oil-type on AR (%) and yield (%). Step-wise regression was performed for development of prediction model for AR (%) and yield (%). Determinant analysis was performed using general linear model (GLM). Our findings indicate that pulp density was the strongest determinant for AR (%), followed by oil dosage and agitation time. Similarly, oil dosage was the primary determinant for yield (%), followed by pulp density and agitation time. Sensitivity analysis was also carried out using artificial neural network (ANN) and the results in respect AR (%) revealed that agitation time was the most important predictor followed by pulp density and oil dosage.

Performance of Drying Oil Modified Chinese Lacquer and Its Gilding Effect

This study explores the modification of traditional Chinese lacquer by incorporating boiled tung oil (BTO), boiled linseed oil (BLO), and turpentine oil (TO) to enhance its properties for gold leafing applications. Current traditional lacquers are limited by slow drying times and inconsistent surface quality, making their performance suboptimal for decorative gilding. The research addresses these gaps by investigating how varying oil types and concentrations (10%, 30%, and 50%) affect the lacquer’s drying time, viscosity, leveling properties, and overall gilding performance. Results indicate that TO-modified lacquer exhibits the best overall performance, showing the fastest drying time, highest glossiness, and smallest color variation, while BTO provides the smoothest surface and BLO ensures the best adhesion. These results demonstrate that the careful selection of oil type and concentration significantly improves lacquer’s functionality for gold leafing, offering a more efficient and aesthetically superior alternative to unmodified lacquers. This study provides valuable insights for optimizing traditional lacquer formulations for modern applications in gilding and decorative finishes.

Managing Maggots in the Eye: A Case Series

Purpose: To describe the presentation and management of ocular maggots and to identify the factors contributing to their occurrence. Study Design: Retrospective case series. Place and Duration of Study: Department of Ophthalmology Mayo Hospital Lahore from 2015 to 2022. Methods: The study involved retrospective chart analysis of patients who presented with Ophthalmomyasis and managed in the eye department. Data regarding age, gender, occupation, clinical findings, co-morbidities, associations and environmental factors were analyzed.Larvae were removed one by one under microscope. Follow up was done after 1 week, 1 month and 3 months. In patients with orbital myasis having distorted, large ulcerated wounds/socket, turpentine oil was used and larvae were removed mechanically under microscope. Results: There were 15 fifteen patients included in the study. Underlying co-morbidities were present in 60% patients and the most common being basal cell carcinoma (46.6%). Seventy four percent patients had some systemic disease; Diabetes Mellitus, Hypertension, AIDS, Hepatitis C etc. One thirdpatients had external ophthalmo-myasis while two third had orbital myasis. Patients who did not complete follow-up were 33%. Use of suffocating agents like turpentine oil, periodic removal of larvae, topical and systemic antibiotics, topical lavage with copious irrigation with sterile fluids resulted in good outcome in all cases that completed the follow up. Conclusion: The majority of patients came from rural communities and had underlying co-morbidities. Timely management resulted in good outcomes for all patients who completed the follow-up, highlighting the importance of comprehensive and sustained treatment protocols.

Brine shrimp cytotoxicity bioassay of red mangrove (Rhizophora mucronata) leaves using different solvents and its potency as antibacterial

Abstract. Zulfahmi I, Paujiah E, Roza ZH, Helmi K, Nafis B, Nur FM, Fazila PN. 2024. Brine shrimp cytotoxicity bioassay of red mangrove (Rhizophora mucronata) leaves using different solvents and its potency as antibacterial. Biodiversitas 25: 3227-3236. This study evaluates the cytotoxicity of ethanol, ethyl acetate, and n-hexane extracts of red mangrove (Rhizopora mucronata) leaves and their potential against pathogenic bacteria. Red mangrove leaf was evaluated for its cytotoxicity using the Brine Shrimp Lethality Test (BSLT). Each solvent's LC50 - 24-hour value was determined using probit analysis and measured using one-way ANOVA with a 95% confidence interval (P<0.05). The secondary metabolic compounds in each extract were presented and analyzed descriptively using GC-MS analysis. The effect of incubation time on the bacterial inhibition zone was tested using independent t-test analysis against S. aureus, V. parahaemolyticus, and A. hydrophyla. The results showed that the LC50 value of ethanol extract was 402.45 ppm, ethyl acetate extract was 230.97 ppm, and n-hexane extract was 180.31 ppm. The secondary metabolite compounds observed in red mangrove leaves extracted with different solvents (ethanol, ethyl acetate, and n-hexane) include terpenoids, alkaloids, ketones, phenols, turpentine oil, essential oils, unsaturated fats, and camphor. The ethyl acetate extract from red mangrove leaves inhibited the growth of S.aureus and V. parahaemolyticus. A. hydrophila and V. parahaemolyticus exhibited the lowest inhibition zone, which varied based on time and concentration.

Optimizing cold-flow properties and oxidation stability of B40 biodiesel blend with turpentine oil and ethanol: Experimental and quantum chemical approach

This study investigates the effectiveness of turpentine oil and ethanol as cold-flow improvers (CFIs), synergizing as antioxidants for ternary B40 biodiesel blend. Utilizing the response surface methodology (RSM), the effects of these CFIs on the ternary blend, including cloud point (CP), cold filter plugging point (CFPP), filter blocking tendency (FBT), precipitation, and oxidation stability, were comprehensively evaluated. The analysis of variance (ANOVA) presented that a linear model best describes the impact of CFIs on CP, whereas quadratic models more accurately represent CFPP, FBT, and oxidation stability. The results also demonstrate that 2FI was identified as the most suitable model for describing precipitation responses. The optimal concentrations of turpentine oil and ethanol were determined to be 10 % v/v and 0.5 % v/v, respectively, resulting in predicted values for CP, CFPP, FBT, precipitation, and oxidation stability of 10.4 °C, 9.0 °C, 1.31, 9.09 mg/100 mL, and 225 minutes, respectively. Theoretical insights from density functional theory (DFT) calculations confirmed that the primary interaction controlling wax formation in biodiesel blends is the strong hydrogen bonding between monostearin and pinene, a major component of turpentine oil, which effectively disrupts wax crystallization. This study presents a novel approach to enhancing B40 biodiesel cold-flow properties and maintaining its oxidation stability by utilizing the synergistic effects of turpentine oil and ethanol to optimize B40 biodiesel formulation.

In Vitro Anti-inflammatory Effects of Larch Turpentine, Turpentine Oil, Eucalyptus Oil, and Their Mixture as Contained in a Marketed Ointment.

An ointment containing larch turpentine, turpentine oil, and eucalyptus oil has been used for almost a century for the symptomatic treatment of mild, localized, purulent inflammations of the skin. Its clinical efficacy in the treatment of skin infections has been shown in clinical trials, but the mode of action of the active ingredients on inflammation is not known. We studied the anti-inflammatory properties of the active ingredients of the ointment and their mixture in a human monocyte cell model, in which the cells were stimulated with lipopolysaccharide and incubated with the test substances. The cytotoxic threshold of each test substance and the mixture was identified using the alamarBlue assay, and their anti-inflammatory activity was assessed by measuring the release of interleukins IL-1β, IL-6, IL-8, monocyte chemoattractant protein-1, prostaglandin E2, and TNF-α. Cell toxicity was observed at a mixture concentration of 10 µg/mL. All immunological assays were carried out at nontoxic concentrations. Larch turpentine decreased IL-1β, monocyte chemoattractant protein-1, and prostaglandin E2 release at a concentration of 3.9 µg/mL and TNF-α at concentrations > 1.95 µg/mL, whereas eucalyptus oil and turpentine oil had no relevant inhibitory effects. The mixture dose-dependently inhibited IL-1β, IL-6, monocyte chemoattractant protein-1, prostaglandin E2, and TNF-α release at concentrations > 1 µg/mL. IL-8 release was only marginally affected. The anti-inflammatory activity of the herbal ingredients and their mixture was confirmed in this model. This effect seems to be mediated mainly by larch turpentine, with turpentine oil and eucalyptus oil exerting an additive or possibly synergistic function.

Phytochemical Composition Antioxidant and Anti-Inflammatory Activity of Artemisia dracunculus and Artemisia abrotanum.

This study aimed to investigate the antioxidant and anti-inflammatory activities mechanism of Artemisia dracunculus (A. dracunculus) and Artemisia abrotanum (A. abrotanum) ethanol extracts in acute rat inflammation induced in Wistar male rats with turpentine oil. The characterization of the polyphenolic compounds in the extracts was conducted using UV-Vis and Fourier-transform infrared spectroscopy and high-performance liquid chromatography coupled with mass spectrometry techniques. The antioxidant activity of the extracts was evaluated in vitro by DPPH, FRAP, H2O2, and NO scavenging tests and in vivo by measuring the total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), 8-hydroxy-deoxyguanosine (8-Oxo-dG), advanced oxidation protein products (AOPP), malondialdehyde (MDA), nitric oxide (NO), 3-nitrotyrosine (3NT), and total thiols (SH). Inflammation was evaluated by measuring nuclear factor-kB-p65 (NfkB-p65) and NLRP3 inflammasome activation with IL-1β, IL-18, and gasdermin D. Liver and renal toxicity was determined following transaminases (ALT and AST), creatinine, and urea. The experimental results indicated that A. dracunculus and A. abrotanum ethanol extracts have moderate in vitro antioxidant activity and had in vivo antioxidant activity and an anti-inflammatory effect by NfkB-p65, IL-1b, IL-18, and gasdermin D serum level reduction. The antioxidant activity correlated with the chemical composition of the extracts. These results bring evidence-based use of A. dracunculus and A. abrotanum's in traditional and contemporary medicine.

Oral Myiasis in Anterior Palate - A Rare Case Report & Review of Literature

Oral myiasis was coined by Reverend F. William Hope in 1840. It is a rare pathology and risk to the patients in humans suffering from myiasis. It has a higher incidence in rural areas and in developing countries having hot climatic conditions such as tropical and sub-tropical zones of Africa and America. Most of the infestations are associated with various predisposing factors such as poor oral hygiene, suppurative lesions, senility, alcoholism, and debilitating conditions and reported in post-extraction socket, trauma. The diagnosis is made basically by the presence of maggots. This article deals with oral myiasis in cerebral palsy female patient. The treatment consisted of manual removal of the maggots by topical application of turpentine oil and surgical debridement of the wound Keywords: Diptera fly, human myiasis, ivermectin, musca domestica, myiasis, parasitic infection

Highly selective rearrangement of β-epoxypinane to perillyl alcohol by ammonium phosphomolybdate bonded on activated carbon

The selective rearrangement of β-epoxypinane is a crucial step in the synthesis of perilla alcohol from inexpensive and readily available turpentine oil. The supported ammonium phosphomolybdate was prepared by chemical adsorption of phosphomolybdate on imidazolated activated carbon and then by ammonia fumigation, which has excellent catalytic performance in the rearrangement of β-epoxypinane to produce perillyl alcohol under mild conditions. The conversion of β-epoxypinane was nearly complete, and the selectivity of perillyl alcohol was as high as 77% in nitromethane at 80 °C for 80 min. The catalyst can be recycled after being washed in hot solvent.

Use of biofuel with turpentine oil additive in a compression ignition engine: a green initiative to fulfill sustainable development goals

Use of biofuel with turpentine oil additive in a compression ignition engine: a green initiative to fulfill sustainable development goals

An Encompassing of two Biofuel – Diesel Blend’s in D.I. Diesel Engine for Reduced Air Pollution Emission

<p>The study explores the usage of Diesel Fuel (DF) blends in conjunction with two biofuels, eucalyptus and turpentine oil, in diesel engines to address the reduction of air pollutants such as Carbon Monoxide (CO), Nitrogen Oxides (NOX), Unburned Hydrocarbons (UBHC), and smoke density. Results indicate that while the use of Eucalyptus Oil Fuel (EOF 40) marginally increases brake-specific energy consumption by 1.03%, Turpentine Oil Fuel (TOF 40) decrease it by 3.05% compared to conventional DF. Furthermore, the Brake Thermal Efficiency (BTE) of TOF 40 blend surpasses that of EOF 40 blends by 4.91%. The analysis of Heat Release Rate (HRR) indicates a higher trend for higher proportions of EOF blend compared to TOF and base DF. TOF blends have lesser atmospheric pollution as compared to EOF blends. Notably, the employment of TOF 40 blend results in a reduction of atmospheric pollutants such as Smoke Density, NOX, CO and UBHC were 12.98%, 17.83%, 25.6% and 15.51%, respectively, in comparison with DF.</p>

Cloud point and oxidation stability analysis of turpentine oil and ethanol in b40 biodiesel blend using response surface methodology

Abstract Given the availability and the potential of bioresources as blending components of diesel blends, the synergy of turpentine oil and ethanol in the B40 biodiesel blend is presented in this work. Response surface methodology (RSM) was employed to study the influence of both bioresources on the important characteristics of B40 biodiesel blends, i.e., cloud point and oxidation stability. Based on the result, the relationship between turpentine oil and ethanol on cloud point was best expressed with a two-factor interaction (2FI) model. Meanwhile, the quadratic model was more suitable for oxidation stability The most ideal cloud point was found at 10.6 °C in 8% v/v and 0.5% v/v of turpentine oil and ethanol concentration, respectively and the most ideal oxidation induction time was found at 250 minutes in 5% v/v and 0.7% v/v of turpentine oil and ethanol, respectively. It is believed that the rigid structure of turpentine oil is responsible for preventing crystal formation and the ethanol-biodiesel blend attraction also enhances their liquid-phase stability. Furthermore, the oxidation stability of B40 biodiesel blends continues to be maintained at sufficient levels.

Development and Evaluation of Celecoxib Emulgel by Using Natural Oil.

Emulgel combines the qualities of an emulsion with those of a gel. In order to create an emulgel w/o or o/w, emulsions have to be formulated, which are then combined with a gelling agent, resulting in a dual-control drug release. Celecoxib exhibits analgesic, antipyretic and anti-inflammatory activities and is used to treat osteoarthritis, severe pain, rheumatoid arthritis, and other medical conditions. Celecoxib Emulgel was developed and evaluated by using natural oil and carbopol- 940 as a gelling agent in different concentrations. The screening of various oils, co-surfactants and surfactants was performed to determine the solubility. The essential oils (eucalyptus oil and turpentine oil) were used as penetration modifiers. Studies on compatibility with polymers have been conducted, and the results indicate that there should be no physical or chemical interactions between the polymers and the drug substance. For the preparation of emulgel, various emulsions were prepared with Smix (cosurfactant and surfactant) ratios (1:1, 2:1 and 3:1). The selection of a gelling agent was done by incorporating the selected emulsion system ratio of 1:1 with the combinations of polymers carbapol 940, carbapol 934, and HPMC (0:1:0, 0:0.5:1, 0:0:3, 0.5:0:1, 1:0:0) gel base to make a homogenous emulgel. The emulgel was examined visually to see if it had any phase behaviour, feel, spreadability, and grittiness by applying its thin layer to a slide. Then, all six formulations of emulgel were prepared with the selected gelling agent. All emulgels were evaluated for pH, physical properties (consistency, homogeneity, colour, texture), drug content, spreadability, extrudability, swelling index, viscosity, stability and centrifugation. A Franz diffusion cell and an egg membrane were used to perform in-vitro drug release. Among all prepared formulations, EG1 had a better release, higher viscosity, higher drug content, and a higher swelling index than the others. The formulation EG1 showed higher drug release (91.25%) within 8 hours.