Spectrophotometric Method For Simultaneous Estimation Research Articles

Application of UV Spectrophotometric Methods for Simultaneous Estimation of Emtricitabine and Tenofovir Alafenamide Fumarate in Bulk

Application of UV Spectrophotometric Methods for Simultaneous Estimation of Emtricitabine and Tenofovir Alafenamide Fumarate in Bulk

Innovative spectrophotometric methods for simultaneous estimation of the novel two-drug combination: Sacubitril/Valsartan through two manipulation approaches and a comparative statistical study

Different innovative spectrophotometric methods were introduced for the first time for simultaneous quantification of sacubitril/valsartan in their binary mixture and in their combined dosage form without prior separation through two manipulation approaches. These approaches were developed and based either on two wavelength selection in zero-order absorption spectra namely; dual wavelength method (DWL) at 226nm and 275nm for valsartan, induced dual wavelength method (IDW) at 226nm and 254nm for sacubitril and advanced absorbance subtraction (AAS) based on their iso-absorptive point at 246nm (λiso) and 261nm (sacubitril shows equal absorbance values at the two selected wavelengths) or on ratio spectra using their normalized spectra namely; ratio difference spectrophotometric method (RD) at 225nm and 264nm for both of them in their ratio spectra, first derivative of ratio spectra (DR1) at 232nm for valsartan and 239nm for sacubitril and mean centering of ratio spectra (MCR) at 260nm for both of them. Both sacubitril and valsartan showed linearity upon application of these methods in the range of 2.5–25.0μg/mL. The developed spectrophotmetric methods were successfully applied to the analysis of their combined tablet dosage form ENTRESTO™. The adopted spectrophotometric methods were also validated according to ICH guidelines. The results obtained from the proposed methods were statistically compared to a reported HPLC method using Student t-test, F-test and a comparative study was also developed with one-way ANOVA, showing no statistical difference in accordance to precision and accuracy.

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LEVOSULPIRIDE AND RABEPRAZOLE SODIUM: A NEW ANALYTICAL Q-ABSORBANCE RATIO APPROACH

Objective: The aim of the study was to develop a precise, accurate, and rapid ultraviolet spectrophotometric method for simultaneous estimation of levosulpiride (LEVO) and rabeprazole sodium (RBS) in the binary mixture and to validate the method as per ICH guidelines.Method: Estimation of LEVO and RBS was performed by Q-absorbance method. Analysis was performed using the ratio of absorbance at two selected wavelengths, one at iso-absorptive point and other is the absorbance maxima of any one of the components. Single scan spectrum and the overlain spectrum conclude that absorbance maxima of LEVO and RBS are 228 and 291.8 nm, respectively, with a coinciding iso-absorptive point at 255 nm. Method uses a ratio of absorbance for assay at 255 and the second wavelength is 291.8 nm, λmax for RBS. It is also applicable at 228 nm, as the second wavelength.Results: Linearity of LEVO and RBS was found to be 25-125 and 4-36 μg/ml, respectively. The accuracy of the LEVO and RBS was found 99.26% and 99.51%, respectively and Sandell’s sensitivity ranged between 0.0238 and 0.594 μg/cm2. Assay of LEVO (75 mg) and RBS (20 mg) in capsule dosage form was found 99.5% and 98.69% w/w, respectively.Conclusions: The developed method for the estimation of LEVO and RBS in binary mixture were found to be simple, accurate, robust, and reproducible. No interference of excipients and the degraded product was found during the estimation. Therefore, the method can be successfully applied for routine quality control analysis.RBS) in the binary mixture and to validate the method as per ICH guidelines.Method: Estimation of LEVO and RBS was performed by Q-absorbance method. Analysis was performed using the ratio of absorbance at two selected wavelengths, one at iso-absorptive point and other is the absorbance maxima of any one of the components. Single scan spectrum and the overlain spectrum conclude that absorbance maxima of LEVO and RBS are 228 and 291.8 nm respectively with a coinciding iso-absorptive point at 255 nm. Method uses ratio of absorbance for assay at 255 and the second wavelength is 291.8 nm, λmax for RBS. It is also applicable at 228 nm, as the second wavelength.Results: Linearity of LEVO and RBS were found to be 25-125and 4-36 μg/ml respectively. Accuracy of the LEVO and RBS was found 99.26 and 99.51% respectively and Sandell’s sensitivity ranged between 0.0238 and 0.594 𝜇g/cm2. Assay of LEVO (75 mg) and RBS (20 mg) in capsule dosage form was found 99.5 and 98.69% w/w respectively.Conclusions: The developed method for the estimation of LEVO and RBS in binary mixture were found to be simple, accurate, robust and reproducible. No interference of excipients and the degraded product were found during the estimation. Therefore the method can be successfully applied for routine quality control analysis.

Simultaneous Equation Spectrophotometric Methods for Simultaneous Estimation of Formoterol Fumarate Dihydrate and Fluticasone Propionate in Pure Drug and Pharmaceutical formulations.

Simultaneous Equation Spectrophotometric Methods for Simultaneous Estimation of Formoterol Fumarate Dihydrate and Fluticasone Propionate in Pure Drug and Pharmaceutical formulations.

Development and Validation of UV Spectrophotometric Methods for Simultaneous Estimation of Amlodipine besylate and Theophylline

The present study describes simple, specific, sensitive, rapid and economical U.V. spectrophotometric methods for simultaneous estimation of amlodipine besylate and theophylline. The absorbance maxima of the drug were found to be239 nm and 272 nm respectively. Method I is based on formation and solving of simultaneous equation known as Vierodt's method. Method II is based on principle of Q-analysis known as absorbance ratio method. Beer's law obeyed in concentration range of 5–30 μg/ml having regression line equation y = 0.041x + 0.017 and y = 0.064x + 0.052 with correlation coefficient 0.999 and.0.997 of amlodipine besylate and theophylline respectively. Different parameter such as linearity, precision, LOD and LOQ, slope and standard deviation were used for validation in both method.

Development and validation of UV spectrophotometric method for Simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs

A new, simple, accurate and sensitive UV-spectrophotometric absorption correction method has been developed for simultaneous determination of Metformin HCl and Empagliflozin in bulk utilizing concept of standard addition. The method is based upon determination of Empagliflozin at 224 nm & Metformin HCl at 230 nm methanol as a solvent. Overlay spectra of both drugs shows absorbance at 227 nm. Linearity was observed in range of 10–50 μg/ml and 1–3 μg/ml for Metformin HCl and Empagliflozin respectively. The correlation coefficient value was found to near to 1. All methods were statistically validated as per ICH guidelines.

Validated Simultaneous UV Spectrophotometric Methods for estimation of Tramadol Hydrochloride and ketorolac Tromethamine in bulk and marketed Capsule Formulation

The present study describes new, simple, rapid and novel spectrophotometric methods for simultaneous estimation of Tramadol Hydrochloride and Ketorolac Tromethamine combination was analysed by simultaneous equation method (Method B) and First order derivative spectroscopic method (Method C). Method A involved measurement of absorbance at two wavelengths, 271 nm and 246 nm, λmax of tromethamine respectively. For method B, wavelengths 331 nm (for determination of Ketorolac tromethamine) and 271 nm were used. For method C, wavelengths 339.2 nm (Zero crossing of Tramadol hydrochloride) and 229.4 nm (Zero crossing of Ketorolac tromethamine) were used for determination of Ketorolac tromethamine and Tramadol hydrochloride respectively. Beer's law was obeyed in concentration range of 5–50 μg/ml and 2–20 μg/ml for Tramadol hydrochloride and Ketorolac tromethamine respectively by all the methods. Method C was found to be more simple and sensitive. The proposed methods are pharmaceutical formulations due to their specificity, rapidity, simplicity and accuracy. These methods validated for linearity, accuracy and precision as per ICH guidelines.

Ultraviolet spectrophotometric method: Not possible for the simultaneous estimation of active constituents in Phyllanthus amarus

Objective: Our objective was to develop and validate UV spectrophotometric method for simultaneous estimation of phyllanthin, hypophyllanthin, and niranthin in Phyllanthus amarus. P. amarus is highly valued in the treatment of liver ailments and has been shown to posses antihepatitis B virus surface antigen activity. Several analytical methods including high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) are reported for respective phytoconstituents. However, there is no simultaneous estimation of phyllanthin, hypophyllanthin, and niranthin, active constituent of P. amarus . Hence, attempts have been made to develop UV spectrophotometric method because of the simplicity and economical advantage of this technique over other analytical techniques as HPTLC, HPLC, etc. Materials and Methods: Standard solution of phyllanthin, hypophyllanthin, and niranthin were prepared in methanol and scanned under a Shimadzu UV spectrophotomer, under full UV (ultraviolet) range (200-400 nm) to get the spectra. Result: After some experiments, it was found that it is impossible to quantify phyllanthin, hypophyllanthin, and niranthin simultaneously by UV spectrophotometric method. This was evident from the UV spectra as they were overlapping with each other. Conclusion: Same class of drugs may have almost the same functional groups, and gradient reverse-phase liquid chromatography and HPTLC will be more useful to separate such complicated mixtures. Therefore, we propose to develop either HPLC or HPTLC method for simultaneous estimation of these compounds.

Development and Validation of UV-Spectroscopic Method for Estimation of Niacin in Bulk and Pharmaceutical Dosage Form

Validated UV-Visible Spectrophotometric method for simultaneous estimation of Cefixime and Moxifloxacin in Pharmaceutical Dosage FormS S Pekamwar, T M Kalyankar, B V Tambe, S J Wadher

Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Olmesartan Medoxomil and Chlorthalidone in Bulk and Tablet

Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Olmesartan Medoxomil and Chlorthalidone in Bulk and Tablet

Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Metformin and Glipizide in Tablet Dosage Form

A simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Metformin and Glipizide. The method involved estimation of Metformin and Glipizide by simultaneous equation at 272nm and 232nm respectively in their solution in water. This method was validated with respect to linearity, accuracy, precision, LOD and LOQ. Beer’s law obeyed in the concentration range of 5-25μg/ml and 20-50μg/ml for Metformin and Glipizide respectively with the correlation coefficient of above 0.99. Limit of detection and quantification values were determined to be 0.214μg/ml and 0.649μg/ml for Metformin and 0.608μg/ml and 1.854μg/ml for Glipizide respectively. Mean recovery of Metformin and Glipizide were found to be in the range of 98-102% signifies the accuracy of the method. The method was found to be precise as %RSD was less than 2.

Validation and application of Vierordt's spectrophotometric method for simultaneous estimation of tamoxifen/coenzyme Q10 in their binary mixture and pharmaceutical dosage forms

For the sake of improving patient compliance and sustainability of chemotherapy healthcare system, both TC and CoQ10 were formulated as solid lipid nanoparticles (SLNs). The study was focused on establishing and validating a simple and reproducible spectrophotometric method for simultaneous determination of TC and CoQ10 in their binary mixture or pharmaceutical dosage forms. A new method based on simultaneous estimation of drug mixture without prior separation was developed. Validation parameters were checked with International Conference on Harmonization (ICH) guidelines. The accuracy and reproducibility of proposed method was statistically compared to HPLC. The TC and CoQ10 were quantified at absorptivity wavelengths of 236 nm and 275 nm, respectively. Calibration curves obeyed Beer's law in range of 2–14 µg/ml with a correlation coefficient (R2) of 0.999 in both methanol and simplified simulated intestinal fluid (SSIF). The %means recovery of TC and CoQ10 in pure state or binary mixture at various concentration levels were all around 100%. The low values of SD and %RSD (<2%) confirm high precision and accuracy of the proposed method. Formulated SLNs showed different %means recovery in range 81–92% for TC and 32–59% for CoQ10. The data obtained by applying simultaneous Vierordt's equations showed no statistical significance in comparison to HPLC. Vierordt's method was successfully applied as a simple, accurate, precise, and economical analysis method for estimating TC and CoQ10 concentrations in pure state, binary mixture and pharmaceutical dosage forms.

Validated spectrophotometric method for simultaneous estimation of rosuvastatin calcium and aspirin in tablet dosage forms

The present work was aimed at method development and validation for simultaneous estimation of Rosuvastatin Calcium and Aspirin by UV-Spectrophotometric method in pharmaceutical dosage form. In UV method 0.1N NaoH as solvent and ?max of Rosuvastatin Calcium and Aspirin were found to be 232 nm and 222 nm respectively. Concentration ranges were found to be 4-20µg/mL for both drugs. The R2 values were found to be 0.996 and 0.999 for Rosuvastatin Calcium and Aspirin respectively. The method was validated statistically and by recovery studies. Percentage Assay and Recovery were found to be 95-105% for Rosuvastatin Calcium and Aspirin. LOD and LOQ ranges were found to be 0.177 and 0.539µg/mL and 0.298 and 0.903µg/mL for Rosuvastatin Calcium and Aspirin respectively. This method was validated using ICH guidelines. Key words: Aspirin, Rosuvastatin, UV-Spectrophotometric method, Simultaneous equation, Validation

Validated First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Lansoprazole and Aspirin in Tablet Dosage Forms

The present work was aimed at method development and validation for simultaneous estimation of Lansoprazole and Aspirin by UV-Spectrophotometric method in pharmaceutical dosage form. In UV method Methanol as solvent and λmax of Lansoprazole and Aspirin were found to be 232nm and 222nm respectively. Concentration ranges were found to be 4–20μg/mL for both drugs. The R2 values were found to be 0.996 and 0.999 for Lansoprazole and Aspirin respectively. The method was validated statistically and by recovery studies. Percentage Assay and Recovery were found to be 95–105% for Lansoprazole and Aspirin. LOD and LOQ ranges were found to be 0.177 and 0.539μg/mL and 0.298 and 0.903μg/mL for Lansoprazole and Aspirin repectively. This method was validated using ICH guidelines.

Validated UV-Visible Spectrophotometric method for simultaneous estimation of Cefixime and Moxifloxacin in Pharmaceutical Dosage Form

Article history: A simple, accurate, sensitive, economical and reliable first order derivative spectrophotometric method was developed and validated for the estimation of cefixime and moxifloxacin in pharmaceutical dosage form. The optimum conditions for the analysis of the drugs were established. First order derivative method was developed for quantification of cefixime and moxifloxacin. Spectrum was obtained by dissolving cefixime and moxifloxacin in methanol and water (60:40 v/v); wavelength selected was 260 nm for cefixime and 316 nm for moxifloxacin. The Beer's law was obeyed in the concentration range of 2-12 μg/ml. Results of tablet analysis showed percent relative standard deviation (% RSD) in the range of 0.1576 to 0.2183 for cefixime and moxifloxacin which indicate repeatability of the method respectively. Recoveries do not differ significantly from 100% which show there was no interference from the common excipient used in tablet formulation indicating accuracy and reliability of the method. The method was validated as per ICH guideline and found to be accurate, precise and rugged. It was also validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit

Spectrophotometric method for simultaneous estimation of atazanavir sulfate and ritonavir in tablet dosage form

The Drug Development and Therapeutics (DDT) is an open access peer-reviewed journal committed to publishing high-quality articles in the field of Drug synthesis and development and Therapeutics from industry, Clinicians and Academia.

Development and Validation of Derivative Spectrophotometric Method for Simultaneous Estimation of Lornoxicam and Eperisone in their Synthetic Mixture

A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Lornoxicam (LXM) and Eperisone (EPE) by employing first order derivative zero crossing method in methanol. The first order derivative absorption at 264.5 nm (zero cross point of LXM) was used for quantification of EPE and 254 nm (zero cross point of EPE) for quantification of LXM. The linearity was established over the concentration range of 2–24 μg/ml with correlation coefficient 0.9987 and 0.997, respectively. The mean % recoveries were found to be in the range of 99.69 and 99.13 for LXM and EPE respectively. The developed method has been validated as per ICH guidelines and successfully applied to the estimation of LXM and EPE in their Synthetic Mixture.

Method Development and Validation of Dual Wavelength UV Spectrophotometric Method for Simultaneous Estimation of Paracetamol and Caffeine in Combined Dosage Form by Internal Standard Method

Method Development and Validation of Dual Wavelength UV Spectrophotometric Method for Simultaneous Estimation of Paracetamol and Caffeine in Combined Dosage Form by Internal Standard Method

Development and validation of RP-HPLC, HPTLC and UV-visible spectrophotometric methods for simultaneous estimation of alprazolam and propranolol hydrochloride in their combined dosage form

Three accurate, sensitive and reproducible methods are described for the quantitative determination of alprazolam (ALP) and propranolol hydrochloride (PNL) in their combined dosage form. The first method involves an RP-HPLC separation on the C18 column using acetonitrile-25 mM ammonium acetate buffer and 0.2% triethylamine (pH of buffer adjusted to 4 with glacial acetic acid) in the ratio of 35: 65 (v/v) as mobile phase. Symmetrical peaks with good separation, ALP at 9.3 min and PNL at 3.5 min, were achieved. Quantification was done with photo diode array detection at 255 nm over the concentration ranges of 0.5–50 and 10–250 μg/mL for ALP and PNL, respectively. The second method is based on the separation of drugs by HPTLC using chloroform-methanol-ammonia 7: 0.8: 0.1 (v/v/v) as mobile phase. Quantification was achieved using UV detection at 248 nm over the concentration range of 100–600 ng/spot and 5–30 μg/spot for ALP and PNL, respectively. The third method involves dual wavelength UV-visible spectrophotometric method. It is based on the determination of PNL at 319.4 nm using its absorptivity value and ALP at 258.2 nm after deduction of absorbance due to PNL. Quantification was achieved over the concentration range of 1–40 and 80–200 μg/mL for ALP and PNL, respectively. All methods were validated according to ICH guidelines and successively applied to marketed pharmaceutical formulation, and the results of all three methods were compared statistically as well. No interference from the tablet excipients was found.

Spectrophotometric Method For Simultaneous Estimation of Montelukast Sodium and Ebastine in Bulk and Their Combined Tablet Dosage Form

Spectrophotometric Method For Simultaneous Estimation of Montelukast Sodium and Ebastine in Bulk and Their Combined Tablet Dosage Form