Light Of Specific Wavelength Research Articles

Photodynamic Therapy for Colorectal Cancer: An Update and a Look to the Future.

This review provides an update on the current state of photodynamic therapy (PDT) for colorectal cancer (CRC) and explores potential future directions in this field. PDT has emerged as a promising minimally invasive treatment modality that utilizes photosensitizers and specific light wavelengths to induce cell death in targeted tumor tissues. In recent years, significant progress has been made in understanding the underlying mechanisms, optimizing treatment protocols, and improving the efficacy of PDT for CRC. This article highlights key advancements in PDT techniques, including novel photosensitizers, light sources, and delivery methods. Furthermore, it discusses ongoing research efforts and potential future directions, such as combination therapies and nanotechnology-based approaches. By elucidating the current landscape and providing insights into future directions, this review aims to guide researchers and clinicians in harnessing the full potential of PDT for the effective management of CRC.

Modulation of Antioxidant Compounds in Fruits of Citrus reticulata Blanco Using Postharvest LED Irradiation.

Phlegrean mandarin fruits are already known for health-promoting properties due to the high concentration of phytochemicals in peel, pulp, and seed. Biotic and abiotic factors, including light, may modulate their biosynthesis, metabolism, and accumulation. In this context, light-emitting diodes (LED) have recently been applied to control nutritional traits, ripening process, senescence, fruit shelf-life, and pathogenic microbial spoilage of fruits. This study investigated the effect of the seven-day exposure of Phlegrean mandarin fruits to two LED regimes, white (W) and red-blue (RB), to test the possibility that the storage under specific light wavelengths may be used as green preservation technology that enhances fruit phytochemical properties. To pursue this aim, the antioxidant activity and polyphenolic profile of the pulp and peel of mandarins under W and RB light regimes were evaluated and compared with Control fruits not exposed to LED treatment. Our results indicated that storage under W and RB treatments modulates the antioxidant content in pulp and peel differently. Compared to W, the RB regime increases the ascorbic acid, flavonoid, anthocyanin, and carotenoid concentrations, while the polyphenol profile analysis reveals that the number of important phytochemicals, i.e., quercetin rutinoside, chlorogenic acid, sinensetin, and rutin, are higher under W. The overall data demonstrated that postharvest LED irradiation is a valid tool for modifying fruit phytochemical properties, which also boosts specific bioactive compounds.

Sustainable polylactide materials with the function of blocking a specific wavelength of light based on aloe-emodin

Polylactide, a biodegradable polymer, can alleviate white pollution, but the use of polylactide in food packaging is limited by high transmittance to light with a specific wavelength, UV (185–400 nm) and short-wavelength visible (400–500 nm) light. Herein, the polylactide end-capped with renewable light absorber aloe-emodin (PLA-En), is blended with commercial polylactide (PLA) to fabricate the polylactide film with the function of blocking light with a specific wavelength, PLA/PLA-En film. Only 40 % of light around 287 and 430 nm transmits through PLA/PLA-En film incorporating 3 mass% of PLA-En, while the film still maintains good mechanical properties and high transparency more than 90 % at 660 nm because of the good compatibility with PLA. The PLA/PLA-En film exhibits stable light-blocking properties under light irradiation and anti-solvent migration under the immersion of fat simulant. Almost no PLA-En migrated out of the film with the molecular weight of PLA-En only 2.89 × 104 g/mol. Compared with PLA film and commercial PE plastic wrap, the designed PLA/PLA-En film exhibits a better preservative effect on riboflavin and milk for inhibiting the production of 1O2. This study offers a green strategy for developing UV and short-wavelength light protective food package film based on renewable resource.

Near-ultraviolet irradiation to stimulate unmodified polyether ether ketone to achieve stable and sustainable antibacterial activity

ObjectivesThis study aims to investigate the cytotoxicity and sustainable antibacterial activity of unmodified PEEK under specific wavelength light treatment (365 nm), and its antibacterial mechanism was also preliminarily discussed. MethodsA near-ultraviolet source with a wavelength of 365 nm and a power of 5 W were selected. The irradiation time was 30 min, and the distance was 100 mm. A water contact angle tester was used to characterize the surface of the PEEK after 1–15 light treatments. MC3TC-E1 cells were used to evaluate the cytotoxicity of the materials under light treatment. Five kinds of common oral bacteria were detected in vitro, and antibacterial efficiency was determined by colony-forming unit (CFU) and scanning electron microscope (SEM). The antibacterial mechanism of PEEK under light was preliminarily discussed by spectrophotometry. The membrane rupture of Staphylococcus aureus and Escherichia coli was detected by lactate dehydrogenase. Staphylococcus aureus and Staphylococcus mutans were selected for the cyclic antibacterial test. Statistical analysis was performed by one-way analysis of variance and Tukey multiple range test. A significance level of 0.05 was considered (α = 0.05). ResultsThe results of the cell experiment showed that PEEK had no cytotoxicity (P > 0.05). CFU results showed that PEEK had an obvious antibacterial effect on Staphylococcus aureus, Staphylococcus mutans, Staphylococcus gordonii and Staphylococcus sanguis, but had no antibacterial effect on Escherichia coli (P < 0.05). The SEM results also verified the above antibacterial effect. The existence of singlet oxygen was confirmed by spectrophotometry. Meanwhile, the rupture of Staphylococcus aureus membrane was verified by lactate dehydrogenase assay. The water contact angle of the PEEK surface did not change significantly after 15 cycles of light treatment. Cyclic antibacterial experiments showed that the antibacterial effect was sustainable. ConclusionsThis study showed that PEEK has good cytocompatibility with stable and sustainable antibacterial properties under near-ultraviolet. It provides a new idea to solve the non-antibacterial property of PEEK, and also provides a theoretical basis for its further application in dentistry.

New Highly Fluorescent Water Soluble Imidazolium-Perylenediimides: Synthesis and Cellular Response.

The synthesis and characterization of two new water soluble 2,6-bis(imidazolylmethyl)-4-methylphenoxy-containing perylenediimides, PDI-1 and PDI-2, are described. These compounds demonstrate a high fluorescence quantum yield in water and were investigated as potential photosensitizers for generating reactive oxygen species with applications in anticancer activities. The HeLa cell line (VPH18) was used to evaluate their efficacy. Fluorescence microscopy was employed to confirm the successful internalization of PDI-1 and PDI-2, while confocal microscopy revealed the specific locations of both PDIs within the lysosomes and mitochondria. In vitro studies were conducted to evaluate the anticancer activity of PDI-1 and PDI-2. Remarkably, these photosensitizers demonstrated a significant ability to selectively eliminate cancer cells when exposed to a specific light wavelength. The water solubility, high fluorescence quantum yield, and selective cytotoxicity of these PDIs toward cancer cells highlight their potential as effective agents for targeted photodynamic therapy. In conclusion, the findings presented here provide a strong foundation for the future exploration and optimization of PDI-1 and PDI-2 as effective photosensitizers in photodynamic therapy, potentially leading to improved treatment strategies for cancer patients.

Temperature measurements on facial skin surface as evaluated by infrared thermal cam-era and pyrometer following physiotherapeutic light treatments

Any clinical procedure in dentistry, especially one that involves a breach of tissue integrity, carries the risk of complications, which can occur in any speciality. These include: postopera-tive wound pain, tissue swelling, bleeding, redness, elevated temperature, trismus, decreased sensation as a result of nerve damage. Postoperative patient care aims to minimise the risk of complications and to treat those which have developed. To this end, we can resort to physical therapy, one of the modalities of which is light therapy, using electromagnetic wave ranges of red, infrared, yellow and ultraviolet light. Yet, it remains unclear which wavelength should be used to treat any specific disease entity and which form of therapeutic light should be used in the rehabilitation of a specific complication following dental procedures? In this study, we used the Cason CA380 infrared digital pyrometer with a laser pointer and the Fluke Ti 400 thermal imaging camera. On the basis of the tests and statistical analysis, it can be concluded that the application of light significantly increases the temperature of the irradiated facial skin surface. Irrespective of the type of light used, each was associated with an increase in temper-ature. A more pronounced increase in temperature on the facial skin surface after a given ap-plication suggests that the effect of light therapy is shallow, which is relevant to the choice of a specific light wavelength to be applied in a particular disease entity or dental complication.

Determination of the Electrical Parameters of Iodine-Doped Polymer Solar Cells at the Macro- and Nanoscale for Indoor Applications

In this work, macro- and nanodiagnostic procedures for working, third-generation photovoltaic devices based on a modified polymer:fullerene (P3HT:PCBM) absorber were conducted using atomic force microscopy (AFM) and impedance spectroscopy (IS) equipment. All experiments were performed both in the dark and under irradiation with a specific light wavelength. Photoactive Kelvin probe force microscopy (p-KPFM) and impedance spectroscopy (p-IS) experiments were conducted on half- and whole-solar cell devices. Based on the p-KPFM measurements, the surface potential (SP) and surface photovoltage (SPV) on top of the active layer at the micro/nanoscale were estimated for various light wavelengths (red, green, blue, and white). For light in the red spectrum range, which was associated with an optical absorption edge and acceptor states that occurred in the band gap of the P3HT material after doping the donor polymer with iodine, the SPV was measured at levels of 183 mV, 199 mV, and 187 mV for the samples with 0%, 5% and 10% iodine doping, respectively. In addition, a macroscale investigation enabling the determination of the electrical parameters of the studied organic solar cells (OSCs) was carried out using p-IS. Based on the data obtained during p-IS experiments, it was possible to propose a series electrical equivalent circuit to define and describe the charge transfer phenomenon in the OSCs. Estimations of data obtained from the fitting of the experimental results of p-IS under white light allowed us to evaluate the average diffusion time of electric charges at 8.15 µs, 16.66 µs, and 24.15 µs as a function of organic layer thickness for the device without doping and with 5% and 10% iodine doping. In this study, we demonstrated that correlating information obtained at the macro- and nanoscale enabled a better understanding of the electrical charge distribution of OSCs for indoor applications.

A Novel Photopharmacological Tool: Dual-Step Luminescence for Biological Tissue Penetration of Light and the Selective Activation of Photodrugs.

Conventional pharmacology lacks spatial and temporal selectivity in terms of drug action. This leads to unwanted side effects, such as damage to healthy cells, as well as other less obvious effects, such as environmental toxicity and the acquisition of resistance to drugs, especially antibiotics, by pathogenic microorganisms. Photopharmacology, based on the selective activation of drugs by light, can contribute to alleviating this serious problem. However, many of these photodrugs are activated by light in the UV-visible spectral range, which does not propagate through biological tissues. In this article, to overcome this problem, we propose a dual-spectral conversion technique, which simultaneously makes use of up-conversion (using rare earth elements) and down-shifting (using organic materials) techniques in order to modify the spectrum of light. Near-infrared light (980 nm), which penetrates tissue fairly well, can provide a "remote control" for drug activation. Once near-IR light is inside the body, it is up-converted to the UV-visible spectral range. Subsequently, this radiation is down-shifted in order to accurately adjust to the excitation wavelengths of light which can selectively activate hypothetical and specific photodrugs. In summary, this article presents, for the first time, a "dual tunable light source" which can penetrate into the human body and deliver light of specific wavelengths; thus, it can overcome one of the main limitations of photopharmacology. It opens up promising possibilities for the moving of photodrugs from the laboratory to the clinic.

Polarization Entanglement from Parametric Down-conversion with an LED Pump

Spontaneous parametric down-conversion (SPDC) is a reliable platform for entanglement generation. Routinely, a coherent laser beam is an essential prerequisite for pumping the nonlinear crystal. Here we break this barrier to generate polarization-entangled photon pairs by using a commercial light-emitting diode (LED) source to serve as the pump beam. This effect is counterintuitive, as the LED source is of extremely low spatial coherence, which is transferred during the down-conversion process to the biphoton wave function. However, the type-II phase matching condition naturally filters the specific frequency and wavelength of LED light exclusively to participate in SPDC such that localized polarization Bell states can be generated, regardless of the global incoherence over the full transverse plane. In our experiment, we characterize the degree of LED light-induced polarization entanglement in the standard framework of the violation of Bell's inequality. We achieve the Bell value $S=2.33\ifmmode\pm\else\textpm\fi{}0.097$, obviously surpassing the classical bound $S=2$ and thus witnessing the quantum entanglement. Our work can be extended to prepare polarization entanglement by using other natural light sources, such as sunlight and biolight, which holds promise for electricity-free quantum communications in outer space.

Design and preparation of high color rendering red inorganic pigment via red light emission phosphor

In this paper, a new method to improve the color rendering performance of inorganic red pigment by adding phosphor is proposed. The optimal mixing ratios of (Ca,Sr)AlSiN3:Eu2+, (Sr,Ba)2Si5N8:Eu2+ and NaMgPO4:Eu2+ phosphors in the γ-Ce2S3 red material are 20%, 10% and 5%, respectively. At the optimal mixing ratios; L* increased by 12.9%, 3.5% and 3%, separately; a* increased by 4.3%, 4% and 1.6% severally; C* increased by 7.9%, 4.3% and 1.5% in turn. Of course, the different properties of the phosphors themselves make the mixing ratios different. Subsequently, the mechanism of pigment fluorescent materials is further investigated by using Chromatometer, SEM, Fluorescence Photometer, UV–vis Spectrophotometer and Fluorescence Microscope. The results shows that, under the irradiation of sunlight, the fluorescent materials in the fluorescent pigments are excited by the light of specific wavelengths in the sunlight, producing an asymmetric broad-band emission in the visible red region. The light emitted as a second light source is diffusely reflected by the pigment particles, which plays a superimposed effect and finally improves the color rendering ability of the red γ-Ce2S3 pigment that originally relies only on the reflected sunlight energy.

Evaluation of a photodynamic therapy agent using a canine prostate cancer model.

Male dogs can develop spontaneous prostate cancer, which is similar physiologically to human disease. Recently, Tweedle and coworkers have developed an orthotopic canine prostate model allowing implanted tumors and therapeutic agents to be tested in a more translational large animal model. We used the canine model to evaluate prostate-specific membrane antigen (PSMA)-targeted gold nanoparticles as a theranostic approach for fluorescence (FL) imaging and photodynamic therapy (PDT) of early stage prostate cancer. Dogs (four in total) were immunosuppressed with a cyclosporine-based immunosuppressant regimen and their prostate glands were injected with Ace-1-hPSMA cells using transabdominal ultrasound (US) guidance. Intraprostatic tumors grew in 4-5 weeks and were monitored by ultrasound (US). When tumors reached an appropriate size, dogs were injected intravenously (iv) with PSMA-targeted nano agents (AuNPs-Pc158) and underwent surgery 24 h later to expose the prostate tumors for FL imaging and PDT. Ex vivo FL imaging and histopathological studies were performed to confirm PDT efficacy. All dogs had tumor growth in the prostate gland as revealed by US. Twenty-four hours after injection of PSMA-targeted nano agents (AuNPs-Pc158), the tumors were imaged using a Curadel FL imaging device. While normal prostate tissue had minimal fluorescent signal, the prostate tumors had significantly increased FL. PDT wasactivated by irradiating specific fluorescent tumor areas with laser light (672 nm). PDT bleached the FL signal, while fluorescent signals from the other unexposed tumor tissues were unaffected. Histological analysis of tumors and adjacent prostate revealed that PDT damaged the irradiated areas to a depth of 1-2 mms with the presence of necrosis, hemorrhage, secondary inflammation, and occasional focal thrombosis. The nonirradiated areas showed no visible damages by PDT. We have successfully established a PSMA-expressing canine orthotopic prostate tumor model and used the model to evaluate the PSMA-targeted nano agents (AuNPs-Pc158) in the application of FL imaging and PDT. It was demonstrated that the nano agents allowed visualization of the cancer cells and enabled their destruction when they were irradiated with a specific wavelength of light.

Zero-field bandwidth stable, reflection bandwidth controllable polymer- stabilised cholesteric liquid crystals enabled by dynamic covalent boronic ester bonds

ABSTRACT Here, an electrically controllable reflection bandwidth and zero-field bandwidth stable PSCLC is judiciously designed and prepared through integrating dynamic covalent boronic ester bonds into the main-chain polymer network. The modulating effect of dynamic covalent bonding on polymer networks can transform the polymer networks from an elastically deformed network to a plastically deformed network by changing the parameters, such as specific wavelengths of light or temperature. Based on the plastic deformation of the network, the zero-field broadband reflection stable and multiple adjustable reflection bandgaps are realised. This work provides new insights into smart and energy-efficient windows.

Wafer-scale hysteresis-free plasmonic hydrogen sensors based on Pd–Au alloy nanoarrays

The prospective market of hydrogen energy has dramatically boosted the demand for accurate and stable detection of ambient hydrogen. In this work, we fabricated wafer-scale plasmonic hydrogen sensors consisting of periodically distributed Pd–Au alloy nanodiscs by holographic lithography and other semiconductor-compatible techniques, thus obtaining reliable and low-cost sensing devices. Light transmittance measurements at specific light wavelength close to the plasmonic absorption peak position are conducted to investigate the hydrogen sensing performance on account that optical signals afford spark-free operation and high tolerance. Upon alloying Pd with appropriate content of Au, the hysteresis between hydrogen absorption and desorption curves is completely suppressed which greatly improves the sensing accuracy throughout the hydrogen pressure range of 102–105 Pa. Moreover, the precisely defined Pd–Au alloy nanodiscs provide satisfactory stability and device-to-device reproducibility. This work demonstrates an attractive route into practical applications of hydrogen detection for the facile fabrication method and satisfactory performance.

Engineering nanoenzymes integrating Iron-based metal organic frameworks with Pt nanoparticles for enhanced Photodynamic-Ferroptosis therapy

Photodynamic therapy (PDT), as a promising strategy in cancer treatment that utilizes photosensitizers (PSs) to produce reactive oxygen species, has been widely used for eliminating cancer cells under specific wavelength light irradiation. However, the low aqueous solubility of PSs and special tumor microenvironments (TME), such as high glutathione (GSH) and tumor hypoxia remain challenges towards PDT for hypoxic tumor treatment. To address these problems, we constructed a novel nanoenzyme for enhanced PDT-ferroptosis therapy by integrating small Pt nanoparticles (Pt NPs) and near-infrared photosensitizer CyI into iron-based metal organic frameworks (MOFs). In addition, hyaluronic acid was adhered to the surface of the nanoenzymes to enhance the targeting ability. In this design, MOFs act not only as a delivery vector for PSs, but also a ferroptosis inducer. Pt NPs stabilized by MOFs were functioned as an oxygen (O2) generator by catalyzing hydrogen peroxide into O2 to relieve tumor hypoxia and increase singlet oxygen generation. In vitro and in vivo results demonstrated that under laser irradiation, this nanoenzyme could effectively relive the tumor hypoxia and decrease the level of GSH, resulting in enhanced PDT-ferroptosis therapy against hypoxic tumor. The proposed nanoenzymes represent an important advance in altering TME for improved clinical PDT-ferroptosis therapy, as well as their potential as effective theranostic agents for hypoxic tumors.

Faint Echo Extraction from ALB Waveforms Using a Point Cloud Semantic Segmentation Model

As an active remote sensing technology, airborne LIDAR can work at all times while emitting specific wavelengths of laser light that can penetrate seawater. Airborne LIDAR bathymetry (ALB) records an object’s full return waveform, including the water surface, water column, seafloor, and the objects on it. Due to the seawater’s absorption and scattering and the seafloor’s reflectivity effect, the seafloor’s amplitude of seafloor echoes varies greatly. Seafloor echoes with low signal-to-noise ratios are not easily detected using waveform processing methods, which can lead to insufficient seafloor topography depth and incomplete seafloor topography coverage. To extract faint seafloor echoes, we proposed a depth extraction method based on the PointConv deep learning model, called FWConv. The method assumed that spatially adjacent echoes were correlated. We converted all the spatially adjacent multi-frame waveforms into a point cloud. Each point represented a bin value in the waveform, and the points’ properties contained spatial coordinates and the amplitude in the waveform. In the semantic segmentation of these point clouds using deep learning models, we considered not only each centroid’s amplitude, but also its neighboring points’ distance and amplitude. This enriched the centroids’ features and allowed the model to better discriminate between background noise and seafloor echoes. The results showed that FWConv could extract faint seafloor echoes in the experimental area and was not easily affected by noise, and that the correctness reached 99.82%. The number of point clouds increased by 158%, and the seafloor elevation accuracy reached 0.20 m concerning the multibeam echo sounder data.

An Update on Recent Advances of Photodynamic Therapy for Primary Cutaneous Lymphomas.

Primary cutaneous lymphomas are rare non-Hodgkin lymphomas consisting of heterogeneous disease entities. Photodynamic therapy (PDT) utilizing photosensitizers irradiated with a specific wavelength of light in the presence of oxygen exerts promising anti-tumor effects on non-melanoma skin cancer, yet its application in primary cutaneous lymphomas remains less recognized. Despite many in vitro data showing PDT could effectively kill lymphoma cells, clinical evidence of PDT against primary cutaneous lymphomas is limited. Recently, a phase 3 "FLASH" randomized clinical trial demonstrated the efficacy of topical hypericin PDT for early-stage cutaneous T-cell lymphoma. An update on recent advances of photodynamic therapy in primary cutaneous lymphomas is provided.

Photodynamic therapy in dentistry: A literature review

Photodynamic therapy is a relatively new non-invasive treatment modality that involves photosensitizers, specific wavelengths of light, and the generation of singlet oxygen and reactive oxygen species. Applications of photodynamic therapy in dentistry are growing rapidly and is slowly reaching all specialties. The present literature review aims to discuss the basic mechanism of photodynamic therapy, its application in various branches of dentistry and its limitations.

Managing leukoplakia with diode laser: A report of two cases

Oral leukoplakia is the most common precancerous lesion of the oral mucosa. WHO defined it as a lesion which has a white patch or plaque on the oral mucosa that cannot be removed by scraping and cannot be classified clinically or microscopically as another disease process. Management of oral leukoplakia should begin with elimination of predisposing habit. There are currently different approaches in the management of Leukoplakia such as scalpel surgery, systemic carotenoids and antioxidants, electro cauterization, cryotherapy and Lasers. Even though on-surgical options are preferred choice of treatment, it takes time to achieve complete regression of lesion and sometimes may not achieve complete resolution. On the other hand, conventional surgical techniques are invasive and cause trauma to the patient. Excision of precancerous oral lesions using LASER offers comparative advantages over traditional scalpel excision. Laser is an acronym for Light Amplification by stimulated emission of radiation. It is surgical technique which excises the targeted tissue using emission of a monochromatic light of specific wavelength. Because of this property, laser excision of the tissue results in complete bloodless field of surgery, reduced chances of post-surgical inflammation and infections and hence better treatment compliance of the patients towards the surgery. This is one of the important goals for management of oral potentially malignant disorders. We present two case reports of homogeneous leukoplakia treated using diode lasers of wavelength 810nm with follow-up. Both cases did not report any post-operative discomfort or inflammation. The aim of this case report is to highlight the importance of laser excision with minimal complications and better treatment outcomes.

5-aminolevulinic acid-loaded dissolving microneedle array for photodynamic therapy of rheumatoid arthritis on rats

Photodynamic therapy (PDT) is a noninvasive technique that can be used to treat rheumatoid arthritis (RA) by irradiating photosensitizers with specific wavelengths of light to generate reactive oxygen species (ROS), thus leading to targeted cell necrosis. However, efficient delivery of photosensitizers with low side effects is a key issue. We developed a 5-aminolevulinic acid-loaded dissolving microneedle array (5-ALA@DMNA) that can locally and efficiently deliver photosensitizers for RA treatment by PDT. 5-ALA@DMNA was fabricated through a two-step molding process, which was characterized. The effects of 5-ALA-mediated PDT on RA fibroblast-like synoviocytes (RA-FLs) were investigated via in vitro experiments. Adjuvant arthritis rat models were established to evaluate the therapeutic effect of 5-ALA@DMNA-mediated PDT on RA. The results showed that 5-ALA@DMNA could penetrate the skin barrier and efficiently deliver photosensitizers. 5-ALA-mediated PDT can significantly inhibit the migration ability and selectively induce apoptosis of RA-FLs. Moreover, 5-ALA-mediated PDT had a significant therapeutic effect on rats with adjuvant arthritis, which may be related to the upregulation of interleukin (IL)− 4 and IL-10 and downregulation of TNF-α, IL-6, and IL-17. Thus, 5-ALA@DMNA-mediated PDT may be a potential therapy for RA.

The anti-inflammatory effects of photobiomodulation are mediated by cytokines: Evidence from a mouse model of inflammation.

There is an urgent need for therapeutic approaches that can prevent or limit neuroinflammatory processes and prevent neuronal degeneration. Photobiomodulation (PBM), the therapeutic use of specific wavelengths of light, is a safe approach shown to have anti-inflammatory effects. The current study was aimed at evaluating the effects of PBM on LPS-induced peripheral and central inflammation in mice to assess its potential as an anti-inflammatory treatment. Daily, 30-min treatment of mice with red/NIR light (RL) or RL with a 40 Hz gamma frequency flicker for 10 days prior to LPS challenge showed anti-inflammatory effects in the brain and systemically. PBM downregulated LPS induction of key proinflammatory cytokines associated with inflammasome activation, IL-1β and IL-18, and upregulated the anti-inflammatory cytokine, IL-10. RL provided robust anti-inflammatory effects, and the addition of gamma flicker potentiated these effects. Overall, these results demonstrate the potential of PBM as an anti-inflammatory treatment that acts through cytokine expression modulation.