Specific Intervals Research Articles

Simulating the induction welding process of thermoplastic composite materials for aircraft structures

Purpose This paper aims to use two different numerical approaches to simulate the induction welding process for a hybrid thermoplastic material, and the results have been validated experimentally. Design/methodology/approach The first approach used a numerical model that combines electromagnetism, heat transfer and solid mechanics in the same numerical environment using Hexagon Marc software. Simultaneously, a computationally efficient approach combined steady-state electromagnetism results at specific intervals in the Ansys EM suite with heat transfer and solid mechanics in Ansys Workbench. Findings The results from both numerical approaches showed a strong correlation with the experimental findings. Originality/value The current research offers valuable insights into enhancing induction welding procedures within the aerospace industry, as well as across broader industrial applications. The synergistic combination of numerical simulations and experimental validation served as a robust framework for future research endeavors aimed at enhancing the efficiency, reliability and quality of thermoplastic welding techniques.

Mucosal epithelial homeostasis: Reference intervals for skin, gill lamellae and filament for Atlantic salmon and other fish species.

Mucosal barriers are gatekeepers of health and exhibit homeostatic variation in relation to habitat and disease. Mucosal Mapping technology provides an in-depth examination of the dynamic mucous cells (MCs) in fish mucosal barriers on tangential sections, about 90° from the view of traditional histology. The method was originally developed and standardized in academia prior to the establishment of QuantiDoc AS to apply mucosal mapping, now trademarked as Veribarr™ for the analysis of skin, gills and gastrointestinal tracts. Veribarr™ uses design-based stereology for the selection and measurement of cell area (size) (μm2), the volumetric density of MCs in the epithelium (MCD, amount of the epithelia occupied by MCs, in %) and the calculated abundance of the MCs (barrier status or defence activity). MC production was mapped across the skin and gill epithelia in 12 species, discovering that gills consistently have two distinct groups of MCs, one on the lamellae where MCs are few and small and one on the filament where MCs are larger and more abundant. MCs were usually much larger in the skin than in the gills, with the latter requiring fewer and smaller cells for adequate respiration. The difference observed between MCs in gill lamella and gill filament is likely a result of functional demands. In addition, our findings also highlight a variation in the mucosal parameters between the species skin, which cannot be explained by the weight differences, and a potential link between MC distribution and species-specific lifestyles in the gill lamella. This diversity necessitates the development of species and tissue site-specific reference intervals for mucosal health evaluation. Mucosal bivariate reference intervals were developed for MC production, including size (trophy) and calculated defence activity (plasia) in the skin and gills of Atlantic salmon, to contrast new measurements against historical data patterns. The application of mucosal reference intervals demonstrates that stress from parasites and treatments can manifest as changes in mucosal architecture, as evidenced by MC hypertrophy and hyperplasia within the gill lamellae. These reference intervals also facilitate comparisons with wild Atlantic salmon, revealing a somewhat higher MC level in farmed salmon gill lamellae. These findings suggest that MC hyperplasia and hypertrophy in the gills are stress/environmental responses in aquaculture. They also advocate for developing specific mucosal bivariate homeostatic reference intervals in aquaculture to improve fish health and welfare across all farmed species.

Dynamics of measurement-induced state transitions in superconducting qubits

We have investigated temporal fluctuation of superconducting qubits via the time-resolved measurement for an IBM Quantum system. We found that the qubit error rate abruptly changes during specific time intervals. Each high error state persists for several tens of seconds and exhibits an on-off behavior. The observed temporal instability can be attributed to qubit transitions induced by a measurement stimulus. Resonant transition between fluctuating dressed states of the qubits coupled with high-frequency resonators can be responsible for the error-rate change.

Enhancing EEG-Based MI-BCIs with Class-Specific and Subject-Specific Features Detected by Neural Manifold Analysis.

This paper presents an innovative approach leveraging Neuronal Manifold Analysis of EEG data to identify specific time intervals for feature extraction, effectively capturing both class-specific and subject-specific characteristics. Different pipelines were constructed and employed to extract distinctive features within these intervals, specifically for motor imagery (MI) tasks. The methodology was validated using the Graz Competition IV datasets 2A (four-class) and 2B (two-class) motor imagery classification, demonstrating an improvement in classification accuracy that surpasses state-of-the-art algorithms designed for MI tasks. A multi-dimensional feature space, constructed using NMA, was built to detect intervals that capture these critical characteristics, which led to significantly enhanced classification accuracy, especially for individuals with initially poor classification performance. These findings highlight the robustness of this method and its potential to improve classification performance in EEG-based MI-BCI systems.

Evaluation of the Effect of Urea and Nanourea on Growth Pattern of Rainfed Bt Cotton (Gossypium hirsutum L.)

A field experiment was conducted to evaluate the effect of different levels of nitrogen through Nanourea and commercial urea with different time of nitrogen application on Bt Cotton (Gossypium hirsutum L.) Fifteen treatment combinations consisting of five different levels of nitrogen through nano urea and commercial urea such as (125% of RDN through nano urea, 100% of through nano urea, 75% RDN through nano urea, 100% RDN through commercial urea and control plot (No nitrogen)) were applied in three split applications of nitrogen at 30,60,90 DAS (40%,40%,20%), 30,60,90,120 DAS (25%,25%,25%,25%), 30,60,90,120 DAS (30%, 30%,20%,20%) were evaluated in split plot design with three replications. The highest absolute growth rates (AGR) for both plant height and dry matter in the context of application of nitrogen through commercial urea were observed during specific growth intervals: 61-90 days after sowing (DAS) for plant height (1.350 cm day-1 plant-1) and 91-120 DAS for dry matter (2.374 g day-1 plant-1). Similarly, when considering split application of nitrogen, the application of nitrogen at 30,60,90 DAS (40%,40%,20%) showed the highest AGR for plant height (1.054 cm day-1 plant-1) during 61-90 DAS and for dry matter (2.158 g day-1 plant-1) during 91-120 DAS. Furthermore, various growth parameters such as CGR, RGR, NAR, and LAI also exhibited their maximum values under both different levels of nitrogen and the mentioned split application of nitrogen.

Effect of Co-Diet Supplementation on Biodegradation of Polyethylene by Galleria mellonella L. (Lepidoptera: Pyralidae).

Pollution coming from plastic polymers, particularly polyethylene (PE), poses a serious threat to both humans and animals. The biodegradation of plastics facilitated by insects is a crucial and eco-friendly approach that can be employed to combat this global concern. Recently, the larvae of the greater wax moth Galleria mellonella (L.) have been recognized as avid 'plastivores'. The current study was aimed at evaluating the feeding efficiency of G. mellonella larvae on PEs of various densities with a co-diet supplementation of wheat germ + honey and beeswax. The results reveal that maximum PE consumption (9.98 ± 1.25 mg) was recorded in the case of 1.0 mm thick PE after a 24 h interval; however, the same scenario also achieved the greatest reduction in larval weight (27.79 ± 2.02 mg). A significant reduction in PE mass (5.87 ± 1.44 mg) was also observed in 1.0 mm PE when fed beeswax; however, the larvae experienced minimal weight loss (9.59 ± 3.81 mg). The larvae exhibited a higher PE consumption in 1.0 mm PE, indicating that the lower the density of PE, the greater the consumed area. Moreover, the biodegradation levels were notably higher within the 24 h interval. In conclusion, these findings suggest that the density of PEs and the supplementation of the co-diet have an impact on PE biodegradation. Additionally, the utilization of G. mellonella for the biodegradation of PE proves effective when combined with beeswax, resulting in minimal weight loss of the larvae. Our findings offer initial insights into how Galleria mellonella larvae biodegrade polyethylene (PE) of four different densities, along with co-diet supplementation. This approach helps us evaluate how varying densities affect degradation rates and provides a better understanding of the larvae's capabilities. Additionally, our observations at three specific time intervals (24, 48, and 72 h) allow us to identify the time required for achieving degradation rates. Through examining these time points, our method offers valuable insights into the initial phases of plastic consumption and biodegradation.

A case series on small incision cataract surgery + PMMA IOL with primary posterior capsulorrhexis: A safe and cheaper alternative in management of pediatric cataracts

Pediatric cataracts, a significant cause of childhood blindness, present pronounced challenges in low-income countries where conventional phacoemulsification with hydrophobic foldable IOLs may be economically unfeasible. In resource-limited settings, the conventional method of employing phacoemulsification with hydrophobic foldable IOLs is often impractical. This case series explores an economically pragmatic alternative, centering on Small-incision cataract surgery (SICS) paired with rigid polymethyl methacrylate (PMMA) intraocular lenses (IOLs). This study delves into the visual outcomes of SICS with in-the-bag PMMA IOLs, Primary Posterior Capsulorrhexis (PPC), and anterior vitrectomy to mitigate Posterior Capsule Opacification (PCO) formation in the Visual Axis (VA). The evaluation, conducted at specific postoperative intervals spanning from Post-op Day 01 to the 6th month, provides a comprehensive understanding of the efficacy and safety of this approach. Findings underscore PMMA IOLs as both durable and cost-effective substitutes, with an emphasis on SICS with PPC as a reliable technique for cataract removal. This research delivers a practical solution to enhance visual outcomes, tackling the distinct challenges confronted in resource-limited settings and contributing to the broader global endeavor to alleviate preventable childhood blindness.

PSIX-23 Evaluation of number of goats grazing grass-based pasture for estimation of methane emission using GreenFeed system

Abstract The objective of this study was to evaluate the number of goats in pasture grazing on methane (CH4) emissions estimates using the GreenFeed system (GFS). The study involved growing meat goats, approximately 1.2 yr old and body weight (BW) of 23.9 ± 0.63 kg, over seven 2-wk periods. In periods 1, 2, and 3, 24 goats were used (Treatment 1: G-24), while periods 4 and 5 involved 18 goats (Treatment 2: G-18), and periods 6 and 7 used 12 goats (Treatment 3: G-12). The pasture area was divided into three paddocks for grazing, except during gas measurements in a stationary respiration calorimetry system (SCS) room for 2 d, where goats were fed fresh forage from the same pastureland. The GFS was programmed to dispense pellets and measured gas at specific intervals of the day (i.e., at 0700-0800 h, 1300-1400 h, 1900-2000 h, and 0100-0200 h), provided 120 g/d of concentrate pellets to attract goats for gas measurements. Statistical analysis using mixed model procedures of SAS included SCS data from preceding and succeeding periods as covariates for GFS gas data analysis. Daily dry matter (DM) intake was greater (P < 0.001) in G-12 compared with G-24 and G-18, but when expressed g/kg BW, intakes were similar (P = 0.081) across treatments. Conversely, digested DM and gross energy intakes were greater (P < 0.001) in G-24 than in G-18 and G-12 (494, 509, and 577 g/d and SE = 21.3 and 7.91, 8.10, and 9.20 MJ/d and SE = 0.359 for G-24, G-18, and G-12, respectively). Daily CH4 production was unaffected (P = 0.254) by treatment (26.6, 25.2, and 26.1 g/d; SE = 0.819), despite variations in daily GFS visits by goats (3.26, 2.61, and 2.53 visits/d and SE = 0.106 for G-24, G-18, and G-12, respectively; P < 0.001). However, CH4 emissions in g/kg DM intake or digestible DM intake differed among treatments, with G-18 intermediate between G-24 and G-12 (35.6, 33.4, and 32.0 g/kg DM intake, SE = 1.02, P = 0.024; 54.1, 51.2, and 48.9 g/kg digestible DM intake, SE = 1.71, and P < 0.001 for G-24, G-18, and G-12, respectively). Carbon dioxide production, expressed in g/d and g/kg DM intake, was greater in G-12 and G-18 compared with G-24. Heat production ranked greatest in G-12, followed by G-18 and G-24. Regardless of treatment, CH4 emissions in g/d, g/kg DM intake or g/kg digestible DM intake were greater in GFS than in SCS (25.5 and 9.56 g/d, 33.8 and 14.4 g/kg DM intake, and 51.2 and 23.8 g/kg digestible DM intake for GFS and SCS, respectively; P < 0.001). In conclusion, differences in CH4 emissions in g/kg DM intake and digestible DM intake among treatments suggests that number of animals in grazing pasture may influence CH4 emission estimates in goats.

Structural Damage Detection under Ambient Excitation Using Symbolic Three-Order Square Matrix Formed by Specific-Interval-Sampled Time-Domain Signals.

In the structural health monitoring of vibration systems, varying excitation always affects the accuracy of damage identification. The proposed symbolic three-order square matrix damage detection method with the matrix norm as a damage indicator can solve the difficult problem of damage identification under ambient excitation. The new sampling pattern extracts data from signals in the time domain at specific intervals based on the structural properties with the help of the autocorrelation coefficient. Then, the data extracted are converted into symbols and arranged into a three-order square matrix, and the Frobenius norm of the matrix is used for structural damage identification as a reliable damage indicator. In this process, the transmissibility function is employed to eliminate the effects of varying excitation. First, the method was verified by a cracked simply supported beam-a simulated Abaqus model. Then, a wooden truss bridge in the laboratory and an actual engineering scenario under ambient excitation together demonstrated the effectiveness and accuracy of the damage identification method and proved the proposed method to be robust to different types of damage under ambient excitation. Compared with other related methods, this method is more intuitive and efficient.

Enhancing data-driven-based state of health estimation for diverse battery applications through effective feature construction

Data-driven-based State of Health (SOH) estimation methods are popular for their ability to avoid considering the battery's complex aging mechanisms. However, aging features extracted from specific voltage intervals and the SOH estimation models established under specific conditions may no longer be applicable to other battery applications. This work first reveals the necessary conditions for constructing effective aging features in a partial voltage interval, and specifically proposes an applicable method for determining the voltage interval of constructing aging features based on the incremental capacity curve and the voltage curve's shape. The proposed method can be adapted to different battery applications and is suitable for partial charging conditions. Given the different aging patterns present in different battery types and applications, a feature self-scaling -based online learning strategy is developed to reduce the model's reliance on aging datasets and enable it to adapt to real-time battery aging patterns. The proposed methods are validated through both an actual aging experiment and three publicly available aging datasets, showcasing their effectiveness in extending the applicability of the proposed SOH estimation methods for diverse battery applications.

Haemodynamic effect of dexmedetomidine during paediatric kidney transplantation.

Dexmedetomidine is increasingly used for its ability to stabilise haemodynamic status during general anaesthesia. However, there is currently no data on paediatric kidney transplant recipients (pKTR). This study investigates the haemodynamic impact of dexmedetomidine administered perioperatively in pKTR. From 2019 to 2023, a retrospective study was conducted at Nantes University Hospital involving all pKTR under 18 years of age. The study compared intraoperative haemodynamic parameters between patients administered dexmedetomidine during kidney transplantation (DEX group) and those who did not receive it (no-DEX group). Mean arterial pressure (MAP) and heart rate (HR) were monitored throughout the duration of anaesthesia and compared. Graft function was assessed based on creatinine levels and glomerular filtration rate (GFR) at specific intervals. The perioperative use of fluids and vasoactive drugs, as well as their administration within 24 h post-surgery, were analysed. Thirty-eight patients were enrolled, 10 in the DEX group and 28 in the no-DEX group. Intraoperative HR was similar between the groups; however, MAP was higher in the DEX group (mean difference 9, standard deviation (SD, 1-11) mmHg, p = 0.039). No differences were found regarding the use of fluid and vasoactive drug therapy between groups. GFR at 1 month post-transplantation was significantly elevated in the DEX group (p = 0.009). pKTR receiving intraoperative dexmedetomidine exhibited higher perioperative MAP compared to those not administered dexmedetomidine. Additionally, the DEX group demonstrated superior graft function at 1 month. The direct impact of dexmedetomidine on immediate postoperative graft function in pTKR warrants further investigation in a prospective multicentre randomised study.

Serum Interleukin-6 as an Early Indicator of Trauma Complications.

Trauma is a major global health issue, associated with high mortality and complications like inapparent hypoxia, fat embolism syndrome (FES), sepsis, and multiple organ dysfunction syndrome (MODS). Early identification of high-risk patients is crucial but challenging. Serum interleukin-6 (IL-6), a key inflammatory cytokine, has shown potential as a biomarker for predicting adverse outcomes in trauma. IL-6 levels typically increase rapidly following trauma, peaking within 12 to 24 hours. Despite its potential role, there is limited research on the effectiveness of IL-6 as an early marker for trauma-related complications. This study aims to assess whether monitoring serum IL-6 levels at specific intervals after trauma can aid in early risk assessment and predict the development of these complications. This prospective observational cohort study at North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences (NEIGRIHMS) included 119 trauma patients aged 19-65 years, admitted within 12 hours of injury. Venous blood samples (5 mL each) were collected at 12 and 24 hours for IL-6 and C-reactive protein (CRP) analysis. Injury severity score (ISS) was assessed for all the patients upon arrival to the emergency department at NEIGRIHMS and was categorized as mild, moderate, severe, and very severe. Inapparent hypoxia, FES, sepsis, and MODS were assessed using pulse oximetry, Gurd's criteria, quick sequential organ failure assessment (qSOFA) score, and Marshall's multiple organ dysfunction score, respectively. Among the participants, 21.85% developed complications; primarily inapparent hypoxia. Serum IL-6 levels were significantly elevated in individuals with complications at both 12 hours (p < 0.001) and 24 hours (p < 0.001) post-trauma. At the 12-hour mark, serum IL-6 demonstrated a sensitivity of 92.3% and a specificity of 78.5%, with a cut-off value of 37.26 pg/mL. By 24 hours, the sensitivity increased to 96.2% and the specificity to 87.1%, with a cut-off value of 55.08 pg/mL. Patients with MODS had the highest IL-6 levels, with medians of 270.87 pg/mL at 12 hours and 826.10 pg/mL at 24 hours. A strong correlation was observed between serum IL-6 at 24 hours and the ISS(rs = 0.725, p < 0.001). At 12 hours, there was a moderate correlation between serum IL-6 and CRP (rs = 0.488, p < 0.001). By 24 hours, this correlation strengthened to a strong level (rs = 0.749, p < 0.001). The significant association of serum IL-6 levels with both ISSand CRP highlights its potential role in assessing trauma severity. The high sensitivity and specificity of IL-6 at the 24-hour make it a valuable biomarker for the early detection of trauma-related complications.

Quantifying canine tissue stiffness change patterns using shear-wave elastography: implications for time of death estimation

ABSTRACT This study investigates the applicability and reproducibility of shear-wave elastography (SWE) in detecting changes in tissue stiffness of canine carcasses. SWE was performed on six canines by two operators. The target organs were liver, spleen, kidney, and thigh muscles. SWE was performed ante-mortem and up to 48-hours post-mortem at specific intervals. The Mann-Whitney U-test and Spearman correlation coefficient showed insignificant correlation between gender and weight with shear-wave velocity (SWV) in ante-mortem scans. The Kruskal-Wallis H-test showed significant differences in SWV for all organs. Intraclass correlation coefficient (ICC) showed excellent correlation for all target organs except for kidneys. Pairwise comparison showed a similar SWV trend for abdominal organs. Accuracy metrics were calculated for determining the post-mortem interval using SWV values. The liver had the highest accuracy (0.69–0.83) across different post-mortem interval thresholds within the cut-off values of ≥2.0 m/s, 1.8–2.0 m/s, <1.8 m/s indicating ≤6 hours post-mortem, 12–18 hours post-mortem and >18 hours post-mortem. The spleen, kidney and thigh muscle demonstrated moderate-to-poor accuracy within the same cut-off parameters. Post-mortem SWV changes in canine solid organs, and soft tissues, particularly the liver, demonstrate a reproducible pattern, which may have value in determining the post-mortem interval.

The effects of acute aerobic exercise on appetite-regulating parameters and energy intake in males with obesity.

To investigate the effects of moderate-intensity aerobic exercise on appetite control parameters, appetite perceptions, and energy intake in sedentary males with obesity. Eleven males with obesity (body fat percentage 36.5 ± 2.5%, body mass index 35.3 ± 4.2 kg/m2, V̇O2peak 29 ± 3.1 mL·kg-1·min-1) completed two experimental sessions: (1) no exercise (CTRL) and (2) 60 min of moderate-intensity cycling exercise at 60% V̇O2peak (MICT) in a crossover design. Blood analysis included growth differentiation factor 15 (GDF-15), total ghrelin, peptide tyrosine tyrosine3-36 (PYY3-36), total glucagon-like peptide-1 (GLP-1), insulin, and glucose, as well as subjective appetite perceptions were measured in specific intervals. A standard breakfast at 0 h and an ad libitum meal postexercise was provided. GDF-15 (95% confidence interval [CI]: [2.48-27.28] ng/L, p = 0.021) increased immediately following MICT compared to CTRL. However, there were no differences for PYY3-36 (p = 0.480, ), total ghrelin (p = 0.646, ), and total GLP-1 (p = 0.451, ) between sessions. Appetite perceptions (95% CI: [(-20.38)-(-6.16)] mm, p = 0.001) were suppressed following MICT though energy intake was not different between the sessions (95% CI: [(-1904.9)-928.1] kJ, p = 0.480). Sixty minutes of MICT increased GDF-15 while suppressing appetite perceptions in individuals with obesity. There was no energy compensation postexercise.

Introducing a novel fragility index for assessing financial stability amid asset bubble episodes

This paper is devoted to the development of an innovative fragility index designed to capture comprehensively the dynamics of financial stability during periods characterized by asset bubbles. Utilizing a multifaceted methodological framework, the study begins by identifying bubble occurrences and calculating the delta CoVaR systemic risk metric. Building on established macroeconomic methodologies, we then propose an indicator to quantify the impact of financial bubbles on the stability of an emerging market. Specifically, we construct two coincident indicators based on daily observations of financial stability for the twenty most liquid Romanian companies. The first indicator is derived from the delta CoVaR values of these companies, while the second is computed using the residuals of a model that employs the same financial stability metric as the dependent variable, with a binary variable representing the presence of asset bubbles as the explanatory factor. This second coincident indicator tracks financial stability in the absence of bubble effects. The disparity between these two indicators forms the basis for the creation of an index, termed the “bubble fragility index,” which measures the overall susceptibility of financial stability to asset bubbles. In the context of the Romanian market, this study demonstrates that periods marked by asset bubbles are associated with elevated systemic risks. Our findings indicate that the presence of bubbles significantly intensifies financial risk factors, creating conditions conducive to severe market corrections and economic downturns. We identify specific intervals during which fragility reaches peak levels, including June to September 2018, December 2018 to March 2019, March 2020, and March 2022.

Renormalon-based resummation of Bjorken polarised sum rule in holomorphic QCD

Approximate knowledge of the renormalon structure of the Bjorken polarised sum rule (BSR) Γ‾1p−n(Q2) leads to the corresponding BSR characteristic function that allows us to evaluate the leading-twist part of BSR. In our previous work [1], this evaluation (resummation) was performed using perturbative QCD (pQCD) coupling a(Q2)≡αs(Q2)/π in specific renormalisation schemes. In the present paper, we continue this work, by using instead holomorphic couplings [a(Q2)↦A(Q2)] that have no Landau singularities and thus require, in contrast to the pQCD case, no regularisation of the resummation formula. The D=2 and D=4 terms are included in the Operator Product Expansion (OPE) of inelastic BSR, and fits are performed to the available experimental data in a specific interval (Qmin2,Qmax2) where Qmax2=4.74GeV2. We needed relatively high Qmin2≈1.7GeV2 in the pQCD case since the pQCD coupling a(Q2) has Landau singularities at Q2≲1GeV2. Now, when holomorphic (AQCD) couplings A(Q2) are used, no such problems occur: for the 3δAQCD and 2δAQCD variants the preferred values are Qmin2≈0.6GeV2. The preferred values of αs in general cannot be unambiguously extracted, due to large uncertainties of the experimental BSR data. At a fixed value of αsMS‾(MZ2), the values of the D=2 and D=4 residue parameters are determined in all cases, with the corresponding uncertainties.

Need for Community Specific Reference Intervals in Pulmonary Function Tests

Need for Community Specific Reference Intervals in Pulmonary Function Tests

Evolving trends in liver transplantation eligibility: A shift toward inclusivity for older adults at a Canadian Transplant Center

Background: The surge of end-stage liver disease among older individuals challenges traditional age-based criteria for liver transplantation (LT), historically capped at 65 years. Our Canadian center shifted away from using chronologic age as an absolute refusal criterion since 2019, enabling those aged 65 years and older to seek LT. This study aimed to investigate temporal trends in the transplant care cascade for patients aged 65 and older at our center, pre- and post-clinical shift. Methods: A retrospective study in a single Canadian transplant center reviewed LT referrals between 2015 and 2023, analyzing proportions of patients aged 65 and above at each stage. Specific intervals, 2015–2018 and 2019–2023, were defined for pre- and post-comparisons. Results: Among the 1,007 LT referrals, 11% (n = 110) were patients aged ≥65 years, with 74% ( n = 81) of them being referred after 2019. From 2015 to 2023, older patient proportions increased at all stages of the transplant care cascade: referrals (7.4% to 12.6%), evaluations (7.6% to 11.4%), waitlisting (5.6% to 15.4%), and transplantations (5.8% to 17.5%). Post-clinical shift, the proportion of older patients referred nearly doubled (7.5% vs 13.7%; p &lt; 0.05), with a similar increase in transplants (5.7% vs. 11.5%; p &lt; 0.05). Conclusions: Removing the age cap increased older patient engagement in the LT care cascade. This emphasizes the crucial role of actively promoting awareness of evolving LT eligibility criteria. Concerted efforts should focus on improving transplantation accessibility in older patients, ensuring age alone does not impede the process.

Comparative genomics and evolutionary analysis of dengue virus strains circulating in Pakistan.

Dengue fever virus (DENV) poses a significant public health risk in tropical and subtropical regions across the world. Although the dengue fever virus (DENV) exhibits significant genetic diversity and has the potential to evolve, there is a lack of comprehensive research on the comparative genomics and evolutionary dynamics of the virus in Pakistan. Phylogenetic analysis demonstrated the circulation of all four dengue virus serotypes (DENV-1, -2, -3, and -4) with prevalent genotypes III and V within DENV-1, cosmopolitan genotype within DENV-2, genotype III within DENV-3, and genotype I within DENV-4 during 2006-2014. Based on the complete envelope region, genome-wide residue signature and genetic diversity indicate that there is a high level of genetic diversity among DENV-1 strains, while DENV-3 strains exhibit the least genetic diversity. Comparative analysis of all four DENV serotypes revealed that certain codons in DENV-2 and -4 were subject to strong purifying selection, while a few codon sites in the envelope region showed evidence of positive selection. These findings provided valuable insights into the comparative genomics and evolutionary pattern of DENV strains reported from Pakistan. Whether those characteristics conferred a fitness advantage to DENV-1 genotypes within a specific geography and time interval warrants further investigations. The findings of the current study will contribute to tracking disease dynamics, understanding virus transmission and evolution, and formulating effective disease control strategies.

Structural Improvement in Crane Boom Rest

Crane boom rests are critical components that endure both static and dynamic loads, leading to deformation and a shortened lifespan. This paper addresses these issues by proposing a redesigned model with enhanced stiffness and load-bearing capacity. The new design incorporates gussets and Styrene-Butadiene Rubber (SBR) sheets. A series of trials were conducted to analyze various stiffener configurations, with results showing a significant reduction in deformation. The optimal configuration, consisting of three L-shaped stiffeners placed at specific intervals, reduced deformation from 17.2 mm to 2.2 mm. Additionally , the integration of SBR rubber sheets further minimized deformation to 0.15 mm. These modifications demonstrate the effectiveness of the proposed improvements in enhancing the durability and performance of crane boom rests. The findings provide a robust solution for extending the operational life of crane boom rests under dynamic loading conditions. Future research can explore different rubber materials and stiffener designs to further enhance structural integrity and load-bearing capacity.