Recirculating aquaculture, using closed water supply installations, has become increasingly important in recent decades as a most promising technology for integrating the production of fish farming and crop production. One of the most characteristic examples of combining two industrial technologies is aquaponics. This artificial biological system (IBS) allows the two subsystems included in it, fish farming and crop production to complement each other. Food not consumed by fish and excrement are a nutrient medium for plants, which in turn purify the habitat from substances harmful to fish, which is especially important in conditions of water recycling. In order to determine the quantitative and qualitative potential of the forage base of organisms on the second link of the trophic chain of IBS created on the basis of aquaculture in a closed water supply installation, an experiment was conducted, the results of which helped to determine the composition and number of organisms promising for inclusion in the technological regime of further joint cultivation. Methods and devices have been developed to remove from the fish-water tank the undissolved sediment formed during the cultivation of fish for the purpose of studying it, the results of the experiment have been analyzed and formalized, a method for converting the resulting ichthyogenic sediment into the feed potential of more complex IBS has been proposed. The results of a quantitative study of the sediment indicate that more than 15% of the feed used passed into production waste. The specific composition of the sediment, in comparison with the feed used, underwent the following changes: in terms of gross energy, the changes amounted to only 14.6%, the lipid content decreased the most, which amounted to 59.6%, the fiber content increased significantly (by 162.4%). The changes in protein were 21.4%. In general, in terms of specific composition and energy value, the collected and analyzed sediment can be considered as a feed potential for many animals occupying a lower stage of the trophic pyramid. The obtained results will allow determining the composition and calculating the number of organisms promising for inclusion in the technological modes of the integrated technologies.