Species Of Nocardioides Research Articles

Nocardioides hankookensis sp. nov., isolated from soil

A Gram-positive, non-motile, rod-shaped bacterial strain, DS-30(T), was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-30(T) grew optimally at pH 6.0-7.0 and 25 degrees C in the presence of 0-0.5 % (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-30(T) fell within the genus Nocardioides, forming a coherent cluster with the type strains of Nocardioides aquiterrae and Nocardioides pyridinolyticus, with which it exhibited 16S rRNA gene sequence similarity values of 98.1 and 98.3 %, respectively. Strain DS-30(T) exhibited 16S rRNA gene sequence similarity values below 96.8 % with respect to the type strains of the other Nocardioides species. The chemotaxonomic properties of strain DS-30(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16 : 0), C(17 : 1)omega8c and C(18 : 1)omega9c. The DNA G+C content was 71.3 mol%. Strain DS-30(T) exhibited DNA-DNA relatedness levels of 19 and 22 % with respect to the type strains of N. aquiterrae and N. pyridinolyticus, respectively, and could be differentiated from these species with reference to a number of phenotypic characteristics. On the basis of the data obtained, strain DS-30(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides hankookensis sp. nov. is proposed. The type strain is DS-30(T) (=KCTC 19246(T) =CCUG 54522(T)).

Nocardioides halotolerans sp. nov., isolated from soil on Bigeum Island, Korea

A strictly aerobic, Gram-positive, motile, coccoid-shaped, halotolerant actinobacterium (10% NaCl, w/v), designated MSL-23 T, was isolated from a soil sample on Bigeum Island, Korea. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Nocardioides, with the highest sequence similarity (95.63%) being to Nocardioides kribbensis KCTC 19038 T. The major menaquinone was MK-8(H 4), and the predominant cellular fatty acids were i-C 16:0, ai-C 17:0, C 18:1 ω9c and 10-methyl-C 16:0. The DNA G+C content was 69.7 mol%. The 16S rRNA gene sequence of strain MSL 23 T and its chemotaxonomic properties showed it to be unique in the genus Nocardioides. Phenotypic characteristics distinguished strain MSL-23 T from other Nocardioides species. On the basis of the phenotypic, chemotaxonomic and phylogenetic data strain MSL-23 T represents a novel species, for which the name Nocardioides halotolerans sp. nov. is proposed, with MSL-23 T (=KCTC 19274 T=DSM 19273 T) as the type strain.

Nocardioides hwasunensis sp. nov.

Two novel actinomycete strains, designated HFW-18 and HFW-21(T), were isolated from a water sample around Hwasun Beach on the coast of Jeju Island, Republic of Korea. The cells of the organisms were aerobic, Gram-positive, non-motile rods. The temperature and initial pH ranges for growth were 4-37 degrees C and pH 5.1-9.1, respectively. They had LL-diaminopimelic acid in their cell-wall peptidoglycan, MK-8(H(4)) as the major menaquinone and DNA G+C contents of 71.1-72.2 mol%. The cellular fatty acids consisted of straight-chain saturated, branched, monounsaturated and 10-methyl fatty acids, with a major component of iso-C(16:0). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The isolates were identical in terms of their 16S rRNA gene sequences and BOX-PCR DNA fingerprints. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates occupied distinct positions within the radiation of the genus Nocardioides. According to 16S rRNA gene sequence similarity, the closest relatives were Nocardioides ganghwensis JC2055(T) (97.4%), Nocardioides oleivorans DSM 16090(T) (97.3%) and Nocardioides furvisabuli SBS-26(T) (97.0%). Levels of 16S rRNA gene sequence similarity between the isolates and other members of the genus Nocardioides were in the range 91.8-95.3%. On the basis of the phenotypic and molecular genetic data presented here, the isolates represent members of a novel species of the genus Nocardioides. The name Nocardioides hwasunensis sp. nov. is proposed, and the type strain is HFW-21(T) (=KCTC 19197(T)=DSM 18584(T)).

Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera)

A Gram-positive, rod-shaped or coccoid, yellow-pigmented bacterial strain, D287(T), was isolated from the water flea Daphnia cucullata (Crustacea: Cladocera) collected from Lake Balaton in Hungary. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons revealed that the strain represented a distinct lineage within the cluster of the genera Nocardioides and Marmoricola. The following characteristics were consistent with the affiliation of strain D287(T) to the genus Nocardioides: peptidoglycan based on LL-2,6-diaminopimelic acid, MK-8(H(4)) as the major menaquinone, iso-C(16:0) as the predominant cellular fatty acid, the presence of phosphatidylglycerol and diphosphatidylglycerol and a DNA G+C content of 69.9 mol%. Owing to characteristic differences in physiological traits and levels of 16S rRNA gene sequence similarity to its phylogenetically closest neighbours that were below 97%, strain D287(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daphniae sp. nov. is proposed. The type strain is D287(T) (=DSM 18664(T)=CCM 7403(T)).

Nocardioides marinisabuli sp. nov., a novel actinobacterium isolated from beach sand

The taxonomic position of a novel actinobacterium (designated SBS-12(T)), which was isolated from beach sand on Jeju Island, Republic of Korea, was determined following a polyphasic taxonomic characterization. Cells of the organism were Gram-positive, catalase-positive, oxidase-negative and non-motile rods. The organism contained ll-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H(4)) as predominant menaquinone, and had a polar lipid profile containing phosphatidylglycerol and phosphatidylinositol, iso-C(16 : 0) as the major fatty acid and a DNA G+C content of 73.1 mol%. A neighbour-joining tree based on 16S rRNA gene sequences showed that the organism occupies a distinct phylogenetic position within the radiation including representatives of the family Nocardioidaceae. High levels of 16S rRNA gene sequence similarity were found with Nocardioides kribbensis (97.2 %), Nocardioides aquiterrae (96.9 %), Nocardioides pyridinolyticus (96.6 %) and Nocardioides aquaticus (96.3 %). The combination of morphological and chemotaxonomic characteristics was consistent with classification in the genus Nocardioides, but the isolate can be differentiated from all Nocardioides species with validly published names by a range of physiological properties. The name Nocardioides marinisabuli sp. nov. is proposed for this novel organism, with the type strain SBS-12(T) (=JBRI 2003(T)=KCCM 42681(T)=DSM 18965(T)).

Nocardioides terrigena sp. nov., isolated from soil

A Gram-positive, rod- or coccoid-shaped bacterial strain, DS-17T, was isolated from a soil in Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-17T grew optimally at around pH 8.0 and 30 degrees C in the presence of 0.5-1.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-17T belonged to the genus Nocardioides. The chemotaxonomic properties of strain DS-17T were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, MK-8(H4) was the predominant menaquinone and iso-C16:0, C17:1omega8c and C17:0 were the major fatty acids. The DNA G+C content was 71.5 mol%. Strain DS-17T exhibited 16S rRNA gene sequence similarity values of 94.5-96.9% to the type strains of recognized Nocardioides species. Strain DS-17T could be distinguished from recognized Nocardioides species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DS-17T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides terrigena sp. nov. is proposed. The type strain is DS-17T (=KCTC 19217T=JCM 14582T).

Nocardioides panacihumi sp. nov., isolated from soil of a ginseng field

A novel bacterial strain designated Gsoil 616(T) was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was Gram-positive, strictly aerobic, non-motile, non-spore-forming and rod- or coccoid-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Nocardioides in the family Nocardioidaceae but was clearly separated from established species of this genus. The 16S rRNA gene sequence similarities between strain Gsoil 616(T) and the type strains of Nocardioides species with validly published names ranged from 91.8 to 96.1 %. The G+C content of the genomic DNA was 73 mol%. Phenotypic and chemotaxonomic data [major menaquinone MK-8(H(4)) and major fatty acid iso-C(16 : 0)] supported the affiliation of strain Gsoil 616(T) to the genus Nocardioides. However, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolate from other Nocardioides species. Therefore, strain Gsoil 616(T) represented a novel species within the genus Nocardioides, for which the name Nocardioides panacihumi sp. nov. is proposed. The type strain is Gsoil 616(T) (=KCTC 19187(T) =DSM 18660(T)).

Nocardioides marinus sp. nov.

A Gram-positive, slightly halophilic, rod-shaped bacterium, designated CL-DD14(T), was isolated from seawater of the East Sea, Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CL-DD14(T) belongs to the genus Nocardioides. Levels of 16S rRNA gene sequence similarity between strain CL-DD14(T) and the type strains of recognized species of the genus Nocardioides were low (94.1-97.1 %). Strain CL-DD14 (T) grew over the pH range 6-9 and temperature range 10-40 degrees C. The strain grew at NaCl concentrations of 0.5-8 % (w/v) with optimum growth at 1-3 % and no growth was observed after 3 weeks on nutrient agar without any salts. It contained ll-diaminopimelic acid as the diamino acid in the cell wall. The major isoprenoid quinone was MK-8(H(4)) and the major cellular fatty acids were iso-C(16 : 0) and 10-methyl C(17 : 0). The DNA G+C content was 72.9 mol%. On the basis of phenotypic and phylogenetic data, strain CL-DD14(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides marinus sp. nov. is proposed. The type strain is CL-DD14(T) (=KCCM 42321(T)=DSM 18248(T)).

Nocardioides insulae sp. nov., isolated from soil

A Gram-positive, rod-shaped or coccoid bacterial strain, DS-51(T), was isolated from a soil in Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-51(T) grew optimally at pH 8.0 and 30 degrees C without NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-51(T) forms a distinct line of descent within the radiation enclosed by the genus Nocardioides. The chemotaxonomic properties of strain DS-51(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, MK-8(H(4)) was the predominant menaquinone and iso-C(16 : 0) was the major fatty acid. The DNA G+C content was 71.1 mol%. The 16S rRNA gene sequence of strain DS-51(T) had similarity levels of 92.5-95.1 % with the sequences of the type strains of Nocardioides species. Strain DS-51(T) could be distinguished from other Nocardioides species by differences in some phenotypic characteristics. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain DS-51(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides insulae sp. nov. is proposed. The type strain is DS-51(T) (=KCTC 19180(T)=DSM 17944(T)).

Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima Island, Japan

A short coccoid- or rod-shaped, non-motile actinomycete strain RC825T was isolated from lichen in Izu-Oshima Island, Japan. A polyphasic study was undertaken to establish the taxonomic position of this strain. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain RC825T forms an evolutionary lineage within the radiation of the genus Nocardioides. In particular, it forms a coherent cluster with Nocardioides oleivorans and Nocardioides ganghwensis with high sequence similarity of 98 and 97.5%, respectively. However, DNA–DNA hybridization experiment demonstrated that strain RC825T is distinct from its closest phylogenetic neighbors, N. oleivorans and N. ganghwensis, with 44 and 39% relatedness, respectively. The cell-wall peptidoglycan of strain RC825T contained LL-diaminopimelic acid, indicating wall chemotype I. The predominant menaquinone was MK-8(H4). Strain RC825T had a cellular fatty acid profile containing straight-chain, branched, unsaturated and 10-methyl fatty acids, and the major fatty acid was iso-C16:0. The DNA G+C content of the strain was 74 mol%. On the basis of both phenotypic and phylogenetic evidence, the strain is separated from previously described Nocardioides species, and should be assigned to represent a novel species of the genus Nocardioides, for which the name Nocardioides exalbidus sp. nov. is proposed. The type strain is strain RC825T (=IAM 15416T=CCTCC AA206016T).

Nocardioides furvisabuli sp. nov., isolated from black sand

A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain SBS-26(T), was isolated from a sample of black sand from Samyang Beach on Jeju Island (Republic of Korea) and was subjected to polyphasic characterization to unravel its taxonomic status. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism belongs to the genus Nocardioides but forms a distinct branch at the base of a Nocardioides ganghwensis-Nocardioides oleivorans cluster. The 16S rRNA gene sequence of strain SBS-26(T) showed the highest levels of similarity to those of N. ganghwensis JC2055(T) (97.7 %) and N. oleivorans DSM 16090(T) (97.6 %). The levels of 16S rRNA gene sequence similarity between strain SBS-26(T) and other members of the genera Nocardioides and Marmoricola were in the range 93.0-96.2 %. The following chemotaxonomic characteristics support the phylogenetic association of strain SBS-26(T) with members of the genus Nocardioides: ll-diaminopimelic acid as the principal diamino acid of the peptidoglycan, MK-8(H(4)) as the major menaquinone, iso-C(16 : 0) as the predominant fatty acid and a DNA G+C content of 69.1 mol%. The polar lipids contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain SBS-26(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides furvisabuli sp. nov. is proposed. The type strain is strain SBS-26(T) (=JCM 13813(T)=NRRL B-24465(T)).

Novel alkalitolerant Nocardioides species isolated from an alkaline soil in Korea

Novel alkalitolerant Nocardioides species isolated from an alkaline soil in Korea

Nocardioides lentus sp. nov., isolated from an alkaline soil

Gram-positive, rod- or coccoid-shaped bacterial strains KSL-17T, KSL-18 and KSL-19 were isolated from an alkaline soil in Korea. They were subjected to analysis using polyphasic taxonomy. Strains KSL-17T, KSL-18 and KSL-19 grew optimally at pH 8.0 and 28 degrees C and in the presence of 0.5 % (w/v) NaCl. They were characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C(16 : 0) as the major fatty acid. Their DNA G+C contents were in the range 74.6-74.8 mol%. Strains KSL-17T, KSL-18 and KSL-19 were identical in terms of their 16S rRNA gene sequences and exhibited a mean level of DNA-DNA relatedness of 85-90 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains KSL-17T, KSL-18 and KSL-19 formed a distinct phylogenetic lineage within the genus Nocardioides. The levels of 16S rRNA gene sequence similarity between the three strains and the type strains of Nocardioides species were in the range 92.6-95.2 %. On the basis of phenotypic, genetic and phylogenetic data, strains KSL-17T (= KCTC 19039T = DSM 16315T), KSL-18 and KSL-19 should be classified as members of a novel species of the genus Nocardioides, for which the name Nocardioides lentus sp. nov. is proposed.

Nocardioides oleivorans sp. nov., a novel crude-oil-degrading bacterium

The crude-oil-degrading strain BAS3(T) represents a novel Nocardioides species, according to a taxonomic study. The 16S rRNA gene sequence of strain BAS3(T) was most similar to that of Nocardioides ganghwensis (IMSNU 14028(T); 99% similarity), but the DNA-DNA relatedness to this type strain was only 32%. The physiological properties of strain BAS3(T) differ from those of N. ganghwensis (IMSNU 14028(T)) and other species of Nocardioides. The diamino acid in the cell-wall peptidoglycan of strain BAS3(T) is LL-diaminopimelic acid and the major menaquinone is MK-8(H(4)). The name Nocardioides oleivorans sp. nov. is proposed for the novel Nocardioides species, since its type strain, BAS3(T) (=DSM 16090(T)=NCIMB 14004(T)), is able to degrade crude oil.

Nocardioides kribbensis sp. nov., isolated from an alkaline soil

Three Gram-positive, rod- or coccoid-shaped bacterial strains, KSL-2(T), KSL-5 and KSL-6, were isolated from an alkaline soil in Korea and subjected to a polyphasic taxonomical analysis. These isolates grew optimally at pH 9.0 and 30 degrees C. They were characterized chemotaxonomically as having cell wall peptidoglycan based on ll-2,6-diaminopimelic acid, MK-8(H(4)) as the predominant menaquinone and iso-C(16:0) as the major fatty acid. The DNA G+C content of the isolates was 73-74 mol%. Strains KSL-2(T), KSL-5 and KSL-6 were identical in their 16S rRNA gene sequences and exhibited DNA-DNA relatedness values of 88-93%. Phylogenetic trees based on 16S rRNA gene sequences showed that the three isolates fell within the evolutionary radiation encompassed by the genus Nocardioides. Levels of 16S rRNA gene sequence similarity between the three strains and the type strains of Nocardioides species ranged from 93.6% (with Nocardioides albus) to 97.2% (with Nocardioides aquiterrae). DNA-DNA relatedness levels between the three isolates and N. aquiterrae CJ-14(T) were 8-15%. On the basis of phenotypic, phylogenetic and genetic data, strains KSL-2(T), KSL-5 and KSL-6 were classified in the genus Nocardioides as members of a novel species for which the name Nocardioides kribbensis sp. nov. is proposed, with KSL-2(T) (=KCTC 19038(T)=DSM 16314(T)) as the type strain.

Nocardioides alkalitolerans sp. nov., isolated from an alkaline serpentinite soil in Korea

Four Gram-positive, rod- or coccus-shaped bacterial strains, KSL-1(T), KSL-9, KSL-10 and KSL-12, were isolated from an alkaline serpentinite soil in Korea, and their taxonomic positions were investigated in a polyphasic study. The four strains exhibited no difference in their 16S rRNA gene sequences. Phylogenetic analyses based on 16S rRNA gene sequences showed that the four strains were phylogenetically affiliated to the genus Nocardioides. The four strains had cell-wall peptidoglycan based on ll-diaminopimelic acid as the diamino acid, indicating wall chemotype I. The predominant menaquinone detected in the four strains was MK-8(H(4)). The major fatty acid components were iso-C(16 : 0), 10-methyl-C(18 : 0), C(18 : 1)omega9c and C(17 : 1)omega6c. The DNA G+C contents were 72.4-73.6 mol%. The four strains exhibited 16S rRNA gene sequence similarity levels of 94.0-96.3 % to the type strains of Nocardioides species with validly published names. DNA-DNA relatedness levels between the four strains were 85-91 %. On the basis of phenotypic properties, phylogenetic distinctiveness and genotypic relatedness, strains KSL-1(T), KSL-9, KSL-10 and KSL-12 were classified in the genus Nocardioides as members of a novel species, Nocardioides alkalitolerans sp. nov. The type strain is strain KSL-1(T) (=KCTC 19037(T)=DSM 16699(T)).

Nocardioides aromaticivorans sp. nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments

Seven strains of dibenzofuran (DF)-degrading bacteria isolated from dioxin-polluted environments were characterized. These isolates were able to grow with dibenzofuran as the sole carbon and energy source. During the growth with dibenzofuran, they produced a soluble yellow metabolite that exhibited a unique pH-dependent shift of absorption maxima. Dibenzo- p-dioxin and biphenyl were also degraded with pigment production. The isolates were strictly aerobic and chemoorganotrophic and had Gram-positive, nonmotile, rod-shaped cells. Chemotaxonomic analyses showed that cells contained L, L-diaminopimeric acid in the peptidoglycan, branched-chain fatty acids as major fatty acids, and menaquinone MK-8(H 4) as the sole respiratory quinone. The G+C content of the DNA of the isolates ranged from 72.0 to 72.4 mol%. The 16S rRNA gene sequences of the isolates were very similar to each other (⩾99.8%). The phylogenetic analysis showed that the isolates formed a cluster with species of the genus Nocardioides with Nocardioides simplex and Nocardioides nitrophenolicus as their nearest neighbors. DNA–DNA hybridization studies showed that the isolates showed a hybridization level of less than 55% to any tested species of the genus Nocardioides. Based on these data, Nocardioides aromaticivorans sp. nov. is proposed for the new DF-degrading isolates. The type strain is strain H-1 (IAM 14992, JCM 11674, DSM 15131).

Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment.

A strictly aerobic, non-motile, rod-shaped actinomycete strain, named JC2055T, was isolated from a sediment sample of getbol, the tidal flat of Korea. 16S rDNA analysis revealed that the getbol isolate belonged to the genus Nocardioides with the highest sequence similarity to Nocardioides aquiterrae GW-9T (95.5%). The major menaquinone was MK-8(H4) and the predominant cellular fatty acids were iso-16 : 0 and 17 : 1omega8c. Tuberculostearic acid was absent. The G+C content of DNA was 72 mol%. Based on the morphological, physiological, biochemical and chemotaxonomical data presented in this study, strain JC2055T can be readily differentiated from other validly named Nocardioides species. The name Nocardioides ganghwensis sp. nov. is proposed for the isolate. The type strain is JC2055T (=IMSNU 14028T=KCTC 9920T=JCM 12124T).

Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea.

A bacterial strain, GW-9T, which was isolated from groundwater in Korea, was subjected to a polyphasic taxonomic study using phenotypic characterization and phylogenetic and genetic methods. Phylogenetic analysis based on 16S rDNA sequences showed that strain GW-9T forms an evolutionary lineage within the radiation enclosing Nocardioides species and, in particular, a coherent cluster with Nocardioides pyridinolyticus. The cell-wall peptidoglycan type of strain GW-9T was based on LL-diaminopimelic acid as the diamino acid, indicating wall chemotype I. The predominant menaquinone was MK-8(H4). Strain GW-9T had a cellular fatty acid profile containing straight-chain, branched, unsaturated and 10-methyl fatty acids. The major fatty acid was iso-C(16:0). The DNA G+C content of strain GW-9T was 73 mol%. The 16S rDNA of strain GW-9T was 99.2% similar to that of the type strain of Nocardioides pyridinolyticus and 94.9-96.0% similar to sequences of the type strains of other Nocardioides species. Differences in phenotypic characteristics and genetic distinctiveness indicate that strain GW-9T is separate from previously described Nocardioides species. Therefore, on the basis of the data presented, a novel species of the genus Nocardioides, Nocardioides aquiterrae sp. nov., is proposed. The type strain is strain GW-9T (=KCCM 41647T=JCM 11813T).

Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium.

A p-nitrophenol-degrading bacterial strain was isolated from industrial wastewater. This strain (NSP41T) was identified as a member of the genus Nocardioides from chemotaxonomic characterizations and phylogenetic inference based on 16S rDNA sequence analysis. The G + C content is 71.4 mol%. The diamino acid in the cell-wall peptidoglycan is LL-diaminopimelic acid. The predominant menaquinone is MK-8(H4). The cellular fatty acid profile is similar to those of Nocardioides species. 16S rDNA sequence analysis show that strain NSP41T is the most related to Nocardioides simplex strains with a level of nucleotide similarity of 98.6%. The levels of 16S rDNA similarity between strain NSP41T and other Nocardioides species ranged from 93.8 to 95.1%. This organism is distinguishable from some other Nocardioides species as well as N. simplex strains by DNA-DNA relatedness data. This organism is different from N. simplex strains in some phenotypic characteristics. Therefore, on the basis of the data presented, a new species of the genus Nocardioides, Nocardioides nitrophenolicus, is proposed. The type strain of the new species is strain NSP41T (= KCTC 0457BPT).