Nocardioides furvisabuli sp. nov., isolated from black sand

Abstract

A Gram-positive, rod-shaped, yellow-pigmented actinomycete, designated strain SBS-26(T), was isolated from a sample of black sand from Samyang Beach on Jeju Island (Republic of Korea) and was subjected to polyphasic characterization to unravel its taxonomic status. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism belongs to the genus Nocardioides but forms a distinct branch at the base of a Nocardioides ganghwensis-Nocardioides oleivorans cluster. The 16S rRNA gene sequence of strain SBS-26(T) showed the highest levels of similarity to those of N. ganghwensis JC2055(T) (97.7 %) and N. oleivorans DSM 16090(T) (97.6 %). The levels of 16S rRNA gene sequence similarity between strain SBS-26(T) and other members of the genera Nocardioides and Marmoricola were in the range 93.0-96.2 %. The following chemotaxonomic characteristics support the phylogenetic association of strain SBS-26(T) with members of the genus Nocardioides: ll-diaminopimelic acid as the principal diamino acid of the peptidoglycan, MK-8(H(4)) as the major menaquinone, iso-C(16 : 0) as the predominant fatty acid and a DNA G+C content of 69.1 mol%. The polar lipids contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain SBS-26(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides furvisabuli sp. nov. is proposed. The type strain is strain SBS-26(T) (=JCM 13813(T)=NRRL B-24465(T)).