Mercury concentrations and yields in the Yukon River are the highest of the world's six largest panarctic drainages. Permafrost thaw has been implicated as the main driver of these high values. Alternative sources include mercury released from glacial melt and erosion, atmospheric mercury pollution, or surface mining. To determine the summer source and speciation of mercury across the Yukon River basin within Canada, we sampled water from 12 tributaries and the mainstem during July 2021. The total (unfiltered) mercury concentration in the glacier-fed White River was 57 ng/L, >10 times higher than all other sampled tributaries. The White River's high total mercury concentrations were driven by suspended sediment and persisted ∼300 km downstream of glacierized headwaters. Total mercury concentrations were lowest (typically <2 ng/L) in tributaries downstream of still-water landscape features (e.g., lakes and settling ponds), suggesting these features are effective sinks for sediment-bound mercury. Low total mercury concentrations (∼2 ng/L) were also observed in five tributaries across diverse thawing permafrost landscapes. These results suggest that glacial erosion and meltwater transport, not permafrost, drive enhanced exports of mercury with suspended sediment. Mercury exports may decline as glacial watersheds pass peak water. Other factors, including mercury released from permafrost thaw, are minor components at present.