Species Artibeus Research Articles

A unique parotid gland in Hart's little fruit bat, Enchisthenes hartii.

Hart's little fruit bat, Enchisthenes hartii, is uncommon and, although it has been the subject of recent molecular genetic studies, is little known biologically. Because chiropteran salivary glands vary interspecifically in ways that reflect evolutionary history and ecology, we examined the parotid gland in E. hartii to ascertain the extent to which it resembles homologous glands in species to which this bat presumably is related. The parotid glands were prepared for electron microscopic examination by conventional means. The parotid gland of E. hartii is structurally unique among all previously studied species of bats (> 230 species examined) and other mammals. In contrast to the same gland in other mammals, the parotid gland in E. hartii lacks secretory endpieces. In their place, there is a type of striated duct. Thus, in this species single secretory elements consist of (proceeding in the direction of salivary flow): striated duct--intercalated duct--and a conventionally located striated duct. The proximal ducts possess microvillus-lined intercellular canaliculi, whereas the walls of the distal ducts include occasional dark cells. Some small serous-like granules may be present in the intercalated duct cells. The function(s) and biological role of the unique parotid gland in E. hartii are unknown. Nevertheless, the presence of two sets of striated ducts provides two separate glandular components seemingly capable of electrolyte transport. This might be of adaptive significance in enabling this species to make use of tropical nutrient resources that otherwise would be unavailable. The uniqueness of its parotid glands lends support to the current hypothesis that E. hartii should be classified as a monotypic genus rather than as a species of Artibeus, whose members it resembles morphometrically.

Ultrastructure of the parotid salivary glands in seven species of fruit bats in the genus Artibeus.

In previous studies, we determined that the submandibular glands of five species of Neotropical fruit bats in the genus Artibeus had seromucous granules in their demilune cells with substructures that varied interspecifically in accordance with systematic relationships. Moreover, the striated ducts in these frugivores exhibited structural modifications that apparently are related to the consumption of a diet rich in potassium, but deficient in sodium. We now turn our attention to the parotid gland in a large number of species in this genus to determine if it follows the same structural pattern as does the submandibular gland. Members of seven different species of Artibeus were live-trapped in various Neotropical locations. The parotid glands were extirpated from euthanized bats, fixed in the field, and prepared for electron microscopic examination by conventional means. The parotid glands in all seven species were virtually identical in morphology. The acinar cells (determined to be seromucous on the basis of ultrastructural criteria) contain large numbers of what appear to be vacuoles, but which are a type of secretory granule. These granules have an electron-lucent matrix and may contain one or several circular membranous profiles arranged either concentrically or in a random array. These granules appear to form by progressive dilatation of the termini of Golgi saccules, with the nascent granules finally severing their connection with the Golgi apparatus. Many of the internal membranous profiles are formed simply by invaginations of the limiting membrane of the granule; others may result from indentation of the limiting membrane by protrusions from adjacent granules; the source of multiple internal membranes in certain granules is unclear. The exocytosis of these granules results in the acinar and intercalated duct lumina being filled with an abundance of membranous material. Such extruded membranes are present in some striated ducts, but not in others, suggesting that they are degraded during passage through the duct system. The striated ducts are of conventional appearance, lacking the frondose processes that are prominent in the submandibular glands of Artibeus. The parotid gland in Artibeus shows none of the interspecific ultrastructural variability that characterizes the submandibular gland in bats of this genus. The seromucous acinar cells secrete granules that release phospholipids as well as glycoconjugates into the saliva. Based on the lack of frondose processes with their sodium-transporting portasomes, the striated ducts of the parotid gland are less concerned with electrolyte homeostasis than are those in the submandibular gland.

Venezuelan encephalitis virus infection in neotropical bats. I. Natural infection in a Guatemalan enzootic focus.

A serological survey of 939 Neotropical bats of 22 species from an enzootic focus of Venezuelan encephalitis (VE) virus on the Pacific lowlands of Guatemala during 1971--1975 revealed VE virus specific antibodies in seven species, three belonging to the genus Artibeus. VE virus was isolated from the blood of one Uroderma bilobatum. Antibody frequency was considerably lower in bats than in terrestrial mammals, and tended to vary within any given species from locality to locality and from year to year. At the village of La Avellana where VE virus was most active, antibody rates in the two best-sampled Artibeus species were 0.14 in 1971, 0.11 in 1972, 0.03 in 1973, and 0.11 in 1975. The positive rate of 0.10 over all years at La Avellana for the genus Artibeus suggests that these and possibly other bats regularly are infected by VE virus, and may possibly serve as alternate hosts to maintain virus circulation if most terrestrial animals become immune.