Use of scientific information in evidence-based decision-making is critically important in addressing coastal and ocean management concerns. In an ecosystem-based management context, ensuring that the “right” information (reliable) is available can be particularly challenging as the information often resides in different organizations with different management mandates. Many governmental and intergovernmental organizations have used a range of approaches, including technical advisory committees and working groups, to facilitate multidisciplinary input into the development of appropriate policies and management practices. This study examined the roles of multiple stakeholders participating in technical working groups that assist in decision-making for marine fisheries management in Belize, a coastal country in Central America. Through interviews with members of three working groups – the Spawning Aggregation Working Group, the Managed Access Working Group, and the National Hicatee Conservation and Monitoring Network – and decision-makers in the Belize Fisheries Department, information production processes and pathways for information uptake into policy were investigated. Major characteristics of communication at the science-policy interface associated with each working group were revealed. Important communication enablers and barriers were identified related to the operation of the working groups, such as membership commitment and resource availability, which influenced knowledge exchange within and outside the groups. Based on the results, a set of requirements for the creation and operation of effective working groups was formulated with regard to requisite inputs, the operational processes, and the types and uses of the information outputs of the groups. These requirements serve as a foundation for development of indicators of the effectiveness of working groups in environmental management contexts that support communication and decision-making at science-policy interfaces.