A large NaI(Tl) detection system was developed to address the increasing demand for high detection efficiency and high energy resolution detectors across various applications. Through Geant4 simulation and experimental research, critical characteristics of the detector prototype were examined, encompassing energy response, energy nonlinearity, and energy resolution. Given the substantial impact of variations in Tl concentration and the complex process of photon propagation on the detection performance of large NaI(Tl) crystals, the detection efficiency and energy resolution with the radioactive source placed at different incident spots were studied to evaluate its spatial response uniformity. The results demonstrate that the detector's energy response range spans from 50 keV to 3 MeV with an energy nonlinearity of 1.5%. The developed large NaI(Tl) detection system achieved an overall energy resolution of 7.93%, along with excellent uniformity in its resolution and detection efficiency, satisfying the application requirements for large dimensions and high detection efficiency.