Spatial Reorientation Research Articles

Mechanistic insights into primary biotransformation of diethyl phthalate in earthworm and significant SOD inhibitory effect of esterolytic products

Phthalic acid esters (PAEs) are used as plasticizer or modifier in artificially-manufactured products. Though the rapid biotransformation of phthalates in microbes and plants have been well documented, it is less studied yet in terrestrial animals, e.g. earthworm. In this study, the major biotransformation of diethyl phthalate (DEP) in Eisenia fetida was illustrated using in vitro incubation of earthworm crude enzymes. DEP could be substantially biotransformed into phthalate monoester (MEP) and a small amount of phthalic acid (PA) through esterolysis, which was verified to be driven by endogenous carboxylesterase. Despite the inferior contribution, the oxidation of DEP might also occur under the initiated electron transfer by NADPH coenzyme. The dominant metabolite MEP showed a higher inhibition of superoxide dismutase (SOD) activity than DEP with EC50 of 0.0082 ± 0.0016 mmol/L, so the higher ecological risks of MEP would be marked. The inhibition effect of PA was validated to be even stronger than MEP though it was slightly generated. The direct binding interaction with SOD was proved to be an important molecular event for regulation of SOD activity. Besides the static quenching mechanism, the caused conformational changes including despiralization of α-helix and spatial reorientation of tryptophan were spectrally believed to affect binding and underlie inhibition efficiency of SOD activity.

An adaptive cue selection model of allocentric spatial reorientation.

After becoming disoriented, an organism must use the local environment to reorient and recover vectors to important locations. A new theory, adaptive combination, suggests that the information from different spatial cues is combined with Bayesian efficiency during reorientation. To test this further, we modified the standard reorientation paradigm to be more amenable to Bayesian cue combination analyses while still requiring reorientation in an allocentric (i.e., world-based, not egocentric) frame. Twelve adults and 20 children at ages 5 to 7 years old were asked to recall locations in a virtual environment after a disorientation. Results were not consistent with adaptive combination. Instead, they are consistent with the use of the most useful (nearest) single landmark in isolation. We term this adaptive selection. Experiment 2 suggests that adults also use the adaptive selection method when they are not disoriented but are still required to use a local allocentric frame. This suggests that the process of recalling a location in the allocentric frame is typically guided by the single most useful landmark rather than a Bayesian combination of landmarks. These results illustrate that there can be important limits to Bayesian theories of the cognition, particularly for complex tasks such as allocentric recall.

Separation of geometric and featural information in children’s spatial representation: Evidence from a model selection task

Previous studies in spatial reorientation have found that young children rely mainly on geometric shapes for reorientation and sometimes ignore features in the environment. Theoretical interpretations of children’s reorientation performance are usually attributed to children’s spatial representation of their surrounding environments. The geometric module theory states that featural information is represented separately from geometric shape in young children’s reorientation, whereas the adaptive combination model depicts an integral representation. Reorientation tasks, however, require the recognition of a specific location, and thus how the whole environment is represented remains unknown. The current study, using a model selection task, explored young children’s representation of the whole surrounding environment. A total of 75 children aged 3–5 years participated in the study. In each trial, children observed a large enclosure and were then asked to choose the corresponding model from two small models. The geometric shapes of the enclosure (rectangle vs. rhombus) and the types of distractors (shape distraction vs. feature position distraction) varied. Results showed that all three age groups performed above the chance level in the shape distraction conditions. Children had more difficulty with the feature position distraction conditions than with the shape distraction conditions. When the distractor shared the feature but at an inappropriate position, children’s performance was significantly poorer, especially in the rhombic enclosure. The results provide evidence that young children may represent featural cues separately from geometric shapes.

Study on active response of superoxide dismutase and relevant binding interaction with bioaccumulated phthalates and key metabolites in Eisenia fetida

Phthalic acid esters (PAEs) are a group of widespread persistent organic pollutants in the environment. Though the harmful effect of PAEs including activity inhibition of superoxide dismutase (SOD) to arouse oxidative stress were well documented, the deep insights into mechanisms that are relevant with SOD activity are still lacking. By 7d-cultivation of Eisenia fetida in artificially-polluted soil, the different active responses of SOD in earthworm were shown to PAE congeners. Despite the less bioaccumulation and bioavailability, the di-butyl phthalate (DBP) etc. structurally coupled with longer ester-chains appeared more effective to trigger the up-regulation and then the slight decline of SOD activity. Given the remarkable biotransformation especially for short-chain PAEs, the SOD activity response in earthworm should be regarded as joint effect with their metabolites, e.g. monophthalates (MAEs) and phthalic acid (PA). The in vitro SOD activity was shown with the obvious inhibition of 21.31% by DBP, 88.93% by MBP, and 58.57% by PA respectively when the concentrations were elevated up to 0.03 mM. The SOD activity inhibition confirmed the molecular binding with pollutants as an essential event besides the biological regulation for activity. The binding interaction was thermodynamically exothermic, spontaneous and strengthened primarily by Van der Waals force and hydrogen bonds, and was spectrally diagnosed with the conformational changes including diminution of α-helix content and spatial reorientation of fluorophore tryptophan. As coherently illustrated with the larger fluorescence quenching constants (3.65*104–4.47*104/mol) than DBP, the metabolites should be the priority concern due to stronger activity inhibition and toxicological risks.

Directed Flow of Beta Band Communication During Reorienting of Attention Within the Dorsal Attention Network.

Background: The endogenous allocation of spatial attention to selected environmental stimuli is controlled by prefrontal (frontal eye fields [FEFs]) and parietal (superior parietal lobe [SPL] and intraparietal sulcus [IPS]) regions belonging to the dorsal attention network (DAN) with a subdivision in subsystems devoted to reorienting (or shifting) of attention between locations (SPL) or maintaining attention at contralateral versus ipsilateral locations (ventral IPS [vIPS]). Although previous studies suggested a leading role of prefrontal regions over parietal sites in orienting attention, the spectral signature of communication flow within the DAN for different attention processes is still debated. Methods: We used the directed transfer function (DTF) on magnetoencephalography (MEG) data to examine the causal interaction between prefrontal and parietal regions of the DAN when subjects shifted versus maintained attention to a stream of cued visual stimuli. Results: In the beta band, we found that shift versus stay cues induced stronger connectivity (DTF values) from right FEF to right SPL, in the early phase of reorienting. Conversely, when considering stay versus shift cues, an increase of DTF values and stronger directionality was observed between bilateral vIPS and from right vIPS to FEF. Similar analyses carried out in theta, alpha, and gamma showed no significant frontoparietal increases of DTF for shift versus stay cues, whereas the stay-related increase of DTF observed in beta between ventral parietal areas was preserved in the alpha band. Conclusions: These findings suggest that control processes in DAN regions (in particular between FEF and SPL) can be associated to a beta frequency channel during shift of attention. Impact statement In the present study, we compared the reorienting response to novel stimuli with respect to maintaining response. Results provided new insights into understanding the neural mechanisms of control attention processes by identifying the frequency-specific causal interactions between frontal and parietal regions belonging to the dorsal attention network supporting spatial reorienting response.

Tau Pathology Profile Across a Parietal-Hippocampal Brain Network Is Associated With Spatial Reorientation Learning and Memory Performance in the 3xTg-AD Mouse.

In early Alzheimer's disease (AD) spatial navigation is one of the first impairments to emerge; however, the precise cause of this impairment is unclear. Previously, we showed that, in a mouse model of tau and amyloid beta (Aβ) aggregation, getting lost represents, at least in part, a failure to use distal cues to get oriented in space and that impaired parietal-hippocampal network level plasticity during sleep may underlie this spatial disorientation. However, the relationship between tau and amyloid beta aggregation in this brain network and impaired spatial orientation has not been assessed. Therefore, we used several approaches, including canonical correlation analysis and independent components analysis tools, to examine the relationship between pathology profile across the parietal-hippocampal brain network and spatial reorientation learning and memory performance. We found that consistent with the exclusive impairment in 3xTg-AD 6-month female mice, only 6-month female mice had an ICA identified pattern of tau pathology across the parietal-hippocampal network that were positively correlated with behavior. Specifically, a higher density of pTau positive cells predicted worse spatial learning and memory. Surprisingly, despite a lack of impairment relative to controls, 3-month female, as well as 6- and 12- month male mice all had patterns of tau pathology across the parietal-hippocampal brain network that are predictive of spatial learning and memory performance. However, the direction of the effect was opposite, a negative correlation, meaning that a higher density of pTau positive cells predicted better performance. Finally, there were not significant group or region differences in M78 density at any of the ages examined and ICA analyses were not able to identify any patterns of 6E10 staining across brain regions that were significant predictors of behavioral performance. Thus, the pattern of pTau staining across the parietal-hippocampal network is a strong predictor of spatial learning and memory performance, even for mice with low levels of tau accumulation and intact spatial re-orientation learning and memory. This suggests that AD may cause spatial disorientation as a result of early tau accumulation in the parietal-hippocampal network.

Mobile brain/body imaging of landmark‐based navigation with high‐density EEG

Coupling behavioral measures and brain imaging in naturalistic, ecological conditions is key to comprehend the neural bases of spatial navigation. This highly integrative function encompasses sensorimotor, cognitive, and executive processes that jointly mediate active exploration and spatial learning. However, most neuroimaging approaches in humans are based on static, motion‐constrained paradigms and they do not account for all these processes, in particular multisensory integration. Following the Mobile Brain/Body Imaging approach, we aimed to explore the cortical correlates of landmark‐based navigation in actively behaving young adults, solving a Y‐maze task in immersive virtual reality. EEG analysis identified a set of brain areas matching state‐of‐the‐art brain imaging literature of landmark‐based navigation. Spatial behavior in mobile conditions additionally involved sensorimotor areas related to motor execution and proprioception usually overlooked in static fMRI paradigms. Expectedly, we located a cortical source in or near the posterior cingulate, in line with the engagement of the retrosplenial complex in spatial reorientation. Consistent with its role in visuo‐spatial processing and coding, we observed an alpha‐power desynchronization while participants gathered visual information. We also hypothesized behavior‐dependent modulations of the cortical signal during navigation. Despite finding few differences between the encoding and retrieval phases of the task, we identified transient time–frequency patterns attributed, for instance, to attentional demand, as reflected in the alpha/gamma range, or memory workload in the delta/theta range. We confirmed that combining mobile high‐density EEG and biometric measures can help unravel the brain structures and the neural modulations subtending ecological landmark‐based navigation.

Quo vadis, international long-distance railway services? Evidence from Central Europe

International railway passenger services have seen considerable changes in Europe over the past few decades. Using international railway connections within and reaching outside the V4 countries (the Czech Republic, Hungary, Poland, Slovakia) in 1990–2019 as a sample, our paper explores trends and patterns in that matter in Central Europe. We have found that the characteristic focus on relatively very frequent short or medium-distance connections using the best equipped railway corridors – paralleled by a considerable reduction of the remaining services – has also been clearly in evidence in this part of the world. However, trends and patterns in Central Europe are not necessarily a mirror image of what has occurred in Western Europe. For instance, although a considerable shortening of travel times has indeed been observed, it has been achieved – characteristically – thanks to the improved permeability of state borders after the enlargement of the EU in 2004, with only moderate improvements to infrastructure being made. Central Europe therefore seems to be the prime example of an area in which contextual, political and geopolitical factors are the keys to understanding the dynamics of changes in transport systems. On the other hand, evidence from this region may give rise to some reflections on the possible roles for the European long-distance rail transport in the future. To begin with, our results show 800 km to be the threshold beyond which the competitive position of rail becomes significantly lower. Secondly, competitive pressure from innovative newcomers (Regiojet, LeoExpress, Arriva) clearly makes national incumbents more flexible and pro-active. Thirdly, the noticeable spatial re-orientation of international railway connections from the V4 countries towards Vienna and Munich (at the expense of Berlin) that we have observed is partly related to the appeal of these cities as multimodal gates with excellent locations within European transport networks. It would therefore seem that the railways in this part of Europe have been attempting to become an integral part of the international multi-modal long-distance transport system.

空间再定向中的线索利用

空间再定向中的线索利用

Supplemental Material for An Adaptive Cue Selection Model of Allocentric Spatial Reorientation

Supplemental Material for An Adaptive Cue Selection Model of Allocentric Spatial Reorientation

Do Older and Young Adults Learn to Integrate Geometry While Navigating in an Environment of a Serious Game?

We evaluated the outcomes of an intervention using a serious game designed to be played on iPads for improving spatial reorientation by training users to integrate geometry of the environment, instead of relying solely on featural cues. Using data logged online through a clinical study of using this game, the effect of training among 16 older adults (69.3 ± 6.4 years, 4 males), who played the game repeatedly (self-administered) over a period of 8 weeks, was investigated. The game contains a hexagonal room with 3 objects, textured walls, and grids on the floor, which are removed one by one as the participant played the game. In each level, the room also rotates such that the viewpoint of the user is different from that of the previous level. Participants cannot play a higher level unless they make no mistake during the trials of the lower test level. In addition to data of older adults available from that clinical trial, we recruited 16 young adults (27.3 ± 5.6 years, 4 males) to play the game for 5 sessions and compared their results with those of the older adults. We evaluated the error type made in each test level and the scores for each session among older adults. Further, we compared the frequency of each error type between young and older adults during the test levels that a landmark adjacent to the target was removed over the first 5 sessions. The results of older adults’ performance suggest they learned to make fewer mistakes over the sessions. Also, both young and older adults learned to integrate the geometrical cues rather than relying on the landmark cue adjacent to the target to find the target. Overall, the results indicate the designed hexagonal room game can enhance spatial cognition among all age groups of adults.

Large-scale brain networks underlying non-spatial attention updating: Towards understanding the function of the temporoparietal junction

The temporoparietal junction (TPJ) and related areas are activated when a target stimulus appears at unexpected locations in Posner's spatial-cueing paradigm, and also when deviant stimuli are presented within a series of standard events in oddball paradigms. This type of activation corresponds to the ventral attention network (VAN), for regions defined on the basis of the spatial task. However, involvement of the VAN in object-based updating of attention has rarely been examined. In the present study, we used functional magnetic resonance imaging to investigate brain responses to (i) invalid targets after category-cueing and (ii) neutrally cued targets deviating in category from the background series of pictures. Bilateral TPJ activation was observed in response to invalidly cued targets, as compared to neutrally cued targets. Reference to the main large-scale brain networks showed that peaks of this activation located in the angular gyrus and inferior parietal lobule belonged to the default mode (DMN) and fronto-parietal networks (FPN), respectively. We found that VAN regions were involved only for simple detection activity. We conclude that spatial and non-spatial reorienting of attention rely on different network underpinnings. Our data suggest that DMN and FPN activity may support the ability to disengage from contextually irrelevant information.

Features of setting and strength gain of the foam concrete massif

The paper presents a study of the regularities of the processes occurring in the matrix and aerated concrete mass at the initial time after laying and later, during the strength gain. The colloidal properties that appear in the foam concrete mix begin at the stage of mixing the components containing binder and aggregate particles, and are activated by the mixer blades. In this case, the mixture is transformed by mass transfer of the matrix material onto the foam grid and a new spatial reorientation of the bubbles under conditions of physical and mechanical effects on the thin-film structure of the foam. In technology, it should be taken into account that the processes occurring in the foam concrete mixture can be attributed to two opposite actions occurring simultaneously. First, in a freshly laid foam concrete mixture, as in any thermodynamically unstable system, sedimentation and coalescence of air pores begins, which is especially manifested at forcedly high water-solid ratios. This can lead, if the technology is not followed, to complete stratification of the mixture. Secondly, in the cement slurry, the setting and further formation of the cement stone occurs. A necessary technological condition for product quality is compliance with the optimal water-solid ratio of the mixture and ensuring the beginning of the setting of the foam concrete mass before its stratification.It was also experimentally determined that an increase in the hardening duration of foamcement systems for more than 28 days is impractical, since it was revealed that an increase in the strength of foam concrete is similar to the same process in structural concrete. It is quite possible to achieve the strength required for foam concrete according to GOST 25485-89 within 28 days and even earlier due to the high technological discipline and the use of modern plasticizing additives.

Backgazing: Reverse Time in Modernist Culture

Backgazing: Reverse Time in Modernist Culture

Impaired Hippocampal-Cortical Interactions during Sleep in a Mouse Model of Alzheimer’s Disease

Spatial learning is impaired in humans with preclinical Alzheimer's disease (AD). We reported similar impairments in 3xTg-AD mice learning a spatial reorientation task. Memory reactivation during sleep is critical for learning-related plasticity, and memory consolidation is correlated with hippocampal sharp wave ripple (SWR) density, cortical delta waves (DWs), cortical spindles, and the temporal coupling of these events-postulated as physiological substrates for memory consolidation. Further, hippocampal-cortical discoordination is prevalent in individuals with AD. Thus, we hypothesized that impaired memory consolidation mechanisms in hippocampal-cortical networks could account for spatial memory deficits. We assessed sleep architecture, SWR-DW dynamics, and memory reactivation in a mouse model of tauopathy and amyloidosis implanted with a recording array targeting isocortex and hippocampus. Mice underwent daily recording sessions of rest-task-rest while learning the spatial reorientation task. We assessed memory reactivation by matching activity patterns from the approach to the unmarked reward zone to patterns during slow-wave sleep (SWS). AD mice had more SWS, but reduced SWR density. The increased SWS compensated for reduced SWR density so there was no reduction in SWR number. In control mice, spindles were phase-coupled with DWs, and hippocampal SWR-cortical DW coupling was strengthened in post-task sleep and was correlated with performance on the spatial reorientation task the following day. However, in AD mice, SWR-DW and spindle-DW coupling were impaired. Thus, reduced SWR-DW coupling may cause impaired learning in AD, and spindle-DW coupling during short rest-task-rest sessions may serve as a biomarker for early AD-related changes in these brain dynamics.

The geometry as an eyed fish feels it in spontaneous and rewarded spatial reorientation tasks

Disoriented human beings and animals, the latter both sighted and blind, are able to use spatial geometric information (metric and sense properties) to guide their reorientation behaviour in a rectangular environment. Here we aimed to investigate reorientation spatial skills in three fish species (Danio rerio, Xenotoca eiseni, Carassius auratus) in an attempt to discover the possible involvement of extra-visual senses during geometric navigation. We observed the fish’s behaviour under different experimental procedures (spontaneous social cued task and rewarded exit task), providing them different temporal opportunities to experience the environmental shape (no experience, short and prolonged experience). Results showed that by using spontaneous social cued memory tasks, fishes were not able to take advantage of extra-visual senses to encode the spatial geometry, neither allowing them short time-periods of environmental exploration. Contrariwise, by using a reference memory procedure, during the rewarded exit tasks, thus providing a prolonged extra-visual experience, fishes solved the geometric task, showing also differences in terms of learning times among species.

Spatial reorientation with a geometric array of auditory cues.

A visuocentric bias has dominated the literature on spatial navigation and reorientation. Studies on visually accessed environments indicate that, during reorientation, human and non-human animals encode the geometric shape of the environment, even if this information is unnecessary and insufficient for the task. In an attempt to extend our limited knowledge on the similarities and differences between visual and non-visual navigation, here we examined whether the same phenomenon would be observed during auditory-guided reorientation. Provided with a rectangular array of four distinct auditory landmarks, blindfolded, sighted participants had to learn the location of a target object situated on a panel of an octagonal arena. Subsequent test trials were administered to understand how the task was acquired. Crucially, in a condition in which the auditory cues were indistinguishable (same sound sample), participants could still identify the correct target location, suggesting that the rectangular array of auditory landmarks was encoded as a geometric configuration. This is the first evidence of incidental encoding of geometric information with auditory cues and, consistent with the theory of functional equivalence, it supports the generalisation of mechanisms of spatial learning across encoding modalities.

For humans navigating without vision, navigation depends upon the layout of mechanically contacted ground surfaces.

Navigation can be haptically guided. In specific, tissue deformations arising from both limb motions during locomotion (i.e., gait patterns) and mechanical interactions between the limbs and the environment can convey information, detected by the haptic perceptual system, about how the body is moving relative to the environment. Here, we test hypotheses concerning the properties of mechanically contacted environments relevant to navigation of this kind. We studied blindfolded participants implicitly learning to perceive their location within environments that were physically encountered via walking on, stepping on, and probing ground surfaces with a cane. Environments were straight-line paths with elevated sections where the path either narrowed or remained the same width. We formed hypotheses concerning how these two environments would affect spatial updating and reorientation processes. In the constant pathwidth environment, homing task accuracy was higher and a manipulation of the elevated surface, to be either unchanged or (unbeknown to participants) shortened, biased the performance. This was consistent with our hypothesis of a metric recalibration scaled to elevated surface extent. In the narrowing pathwidth environment, elevated surface shortening did not bias performance. This supported our hypothesis of positional recalibration resulting from contact with the leading edge of the elevated surface. We discuss why certain environmental properties, such as path-narrowing, have significance for how one becomes implicitly oriented the surrounding environment.

The role of learning and environmental geometryin landmark-based spatial reorientationof fish (Xenotoca eiseni).

Disoriented animals and humans use both the environmental geometry and visual landmarks to guide their spatial behavior. Although there is a broad consensus on the use of environmental geometry across various species of vertebrates, the nature of disoriented landmark-use has been greatly debated in the field. In particular, the discrepancy in performance under spontaneous choice conditions (sometimes called "working memory" task) and training over time ("reference memory" task) has raised questions about the task-dependent dissociability of mechanisms underlying the use of landmarks. Until now, this issue has not been directly addressed, due to the inclusion of environmental geometry in most disoriented navigation paradigms. In the present study, therefore, we placed our focus on landmark-based navigation in fish (Xenotoca eiseni), an animal model that has provided fruitful research in spatial reorientation. We began with a test of spontaneous navigation by geometry and landmarks (Experiment 1), showing a preference for the correct corner, even in the absence of reinforced training. We then proceeded to test landmarks without the influence of informative geometry through the use of square environments (Experiment 2-4), varying the numerosity of present landmarks, the distance of landmarks from the target corner, and the type of task (i.e., spontaneous cued memory or reference memory). We found marked differences in landmark-use in the absence of environmental geometry. In the spontaneous memory task, visual landmarks acquired perceptive salience (and attracted the fish) but without serving as a spatial cue to location when they were distal from the target. Across learning in the reference memory task, the fish overcame these effects and gradually improved in their performance, although they were still biased to learn visual landmarks near the target (i.e., as beacons). We discuss these results in relation to the existing literature on dissociable mechanisms of spatial learning.

Spatial reorientation of the carriage of a CNC lathe when reversing

The process of machining on CNC machines of parts of a complex profile with multi-pass shaping schemes is accompanied by multiple changes in the direction of movement of the working bodies (reverse) during cutting and at idle. In this case, the formation of processing errors associated with the spatial reorientation of the carriage during reversal is possible, the cause of which is the mismatch of the vector of the traction force acting along the axis of the lead screw and the resulting resistance forces associated with cutting forces and friction in the guides. Based on the analysis of the forces acting on the carriage, a design and mathematical model is proposed for guides with prismatic and flat guides. Approbation of the proposed calculation method showed a good level of agreement between the calculated and experimental results. The regularities of the influence of the cutting forces, the location of the center of gravity of the support group, its mass and speed after the reverse on the value of the spatial reorientation have been established.